511 research outputs found
Rapid mechanical stimulation of inner-ear hair cells by photonic pressure
Hair cells, the receptors of the inner ear, detect sounds by transducing mechanical vibrations into electrical signals. From the top surface of each hair cell protrudes a mechanical antenna, the hair bundle, which the cell uses to detect and amplify auditory stimuli, thus sharpening frequency selectivity and providing a broad dynamic range. Current methods for mechanically stimulating hair bundles are too slow to encompass the frequency range of mammalian hearing and are plagued by inconsistencies. To overcome these challenges, we have developed a method to move individual hair bundles with photonic force. This technique uses an optical fiber whose tip is tapered to a diameter of a few micrometers and endowed with a ball lens to minimize divergence of the light beam. Here we describe the fabrication, characterization, and application of this optical system and demonstrate the rapid application of photonic force to vestibular and cochlear hair cells
Lipid bilayer mediates ion-channel cooperativity in a model of hair-cell mechanotransduction
Mechanoelectrical transduction in the inner ear is a biophysical process underlying the senses of hearing and balance. The key players involved in this process are mechanosensitive ion channels. They are located in the stereocilia of hair cells and opened by the tension in specialized molecular springs, the tip links, connecting adjacent stereocilia. When channels open, the tip links relax, reducing the hair-bundle stiffness. This gating compliance makes hair cells especially sensitive to small stimuli. The classical explanation for the gating compliance is that the conformational rearrangement of a single channel directly shortens the tip link. However, to reconcile theoretical models based on this mechanism with experimental data, an unrealistically large structural change of the channel is required. Experimental evidence indicates that each tip link is a dimeric molecule, associated on average with two channels at its lower end. It also indicates that the lipid bilayer modulates channel gating, although it is not clear how. Here, we design and analyze a model of mechanotransduction where each tip link attaches to two channels, mobile within the membrane. Their states and positions are coupled by membrane-mediated elastic forces arising from the interaction between the channels' hydrophobic cores and that of the lipid bilayer. This coupling induces cooperative opening and closing of the channels. The model reproduces the main properties of hair-cell mechanotransduction using only realistic parameters constrained by experimental evidence. This work provides an insight into the fundamental role that membrane-mediated ion-channel cooperativity can play in sensory physiology
Can Larvae of Forest Click Beetles (Coleoptera: Elateridae) Feed on Live Plant Roots?
Simple SummaryDetailed natural history information is still lacking for many species of soil-dwelling invertebrates. We asked whether the larvae (wireworms) of two click beetle species, which are most abundant in European boreal forests, feed on live roots of forest plants. The weight of root pieces of downy birch, Scots pine, Norway spruce and wavy hair-grass, offered to wireworms in a laboratory experiment, did not decrease, indicating that these larvae did not consume live root tissues. Therefore, Athous subfuscus and Dalopius marginatus should be excluded from the lists of forest pests damaging tree roots.The life histories of many soil-dwelling invertebrates remain poorly studied. The larvae of two click beetle species, Athous subfuscus and Dalopius marginatus, which are most abundant in European boreal forests, are both classified as omnivorous and are included in lists of root-damaging pests. Nevertheless, we are not aware of any direct proof of their ability (or inability) to consume plant roots. In this study, we asked whether these larvae actually feed on the roots of forest plants in the absence of other food sources. Live roots of boreal forest plants, including trees (Betula pubescens, Picea abies and Pinus sylvestris) and grass (Deschampsia flexuosa), were offered to click beetle larvae in a two-month microcosm experiment. The weight of roots placed in vials with the wireworms did not decrease, indicating that the larvae of these click beetle species did not feed on live roots, even in the absence of other food sources. This suggests that the feeding niches of A. subfuscus and D. marginatus larvae are narrower than previously thought and do not include live plant tissues. Therefore, these click beetle species should be excluded from the lists of forest pests damaging tree roots
Forces between clustered stereocilia minimize friction in the ear on a subnanometre scale
The detection of sound begins when energy derived from acoustic stimuli
deflects the hair bundles atop hair cells. As hair bundles move, the viscous
friction between stereocilia and the surrounding liquid poses a fundamental
challenge to the ear's high sensitivity and sharp frequency selectivity. Part
of the solution to this problem lies in the active process that uses energy for
frequency-selective sound amplification. Here we demonstrate that a
complementary part involves the fluid-structure interaction between the liquid
within the hair bundle and the stereocilia. Using force measurement on a
dynamically scaled model, finite-element analysis, analytical estimation of
hydrodynamic forces, stochastic simulation and high-resolution interferometric
measurement of hair bundles, we characterize the origin and magnitude of the
forces between individual stereocilia during small hair-bundle deflections. We
find that the close apposition of stereocilia effectively immobilizes the
liquid between them, which reduces the drag and suppresses the relative
squeezing but not the sliding mode of stereociliary motion. The obliquely
oriented tip links couple the mechanotransduction channels to this least
dissipative coherent mode, whereas the elastic horizontal top connectors
stabilize the structure, further reducing the drag. As measured from the
distortion products associated with channel gating at physiological stimulation
amplitudes of tens of nanometres, the balance of forces in a hair bundle
permits a relative mode of motion between adjacent stereocilia that encompasses
only a fraction of a nanometre. A combination of high-resolution experiments
and detailed numerical modelling of fluid-structure interactions reveals the
physical principles behind the basic structural features of hair bundles and
shows quantitatively how these organelles are adapted to the needs of sensitive
mechanotransduction.Comment: 21 pages, including 3 figures. For supplementary information, please
see the online version of the article at http://www.nature.com/natur
Background studies for the EDELWEISS dark matter experiment
The EDELWEISS-II collaboration has completed a direct search for WIMP dark
matter using cryogenic Ge detectors (400 g each) and 384 kgdays of
effective exposure. A cross-section of pb is excluded at
90% C.L. for a WIMP mass of 85 GeV. The next phase, EDELWEISS-III, aims to
probe spin-independent WIMP-nucleon cross-sections down to a few
pb. We present here the study of gamma and neutron background
coming from radioactive decays in the set-up and shielding materials. We have
carried out Monte Carlo simulations for the completed EDELWEISS-II setup with
GEANT4 and normalised the expected background rates to the measured
radioactivity levels (or their upper limits) of all materials and components.
The expected gamma-ray event rate in EDELWEISS-II at 20-200 keV agrees with the
observed rate of 82 events/kg/day within the uncertainties in the measured
concentrations. The calculated neutron rate from radioactivity of 1.0-3.1
events (90% C.L.) at 20-200 keV in the EDELWEISS-II data together with the
expected upper limit on the misidentified gamma-ray events (), surface
betas (), and muon-induced neutrons (), do not contradict 5
observed events in nuclear recoil band. We have then extended the simulation
framework to the EDELWEISS-III configuration with 800 g crystals, better
material purity and additional neutron shielding inside the cryostat. The
gamma-ray and neutron backgrounds in 24 kg fiducial mass of EDELWEISS-III have
been calculated as 14-44 events/kg/day and 0.7-1.4 events per year,
respectively. The results of the background studies performed in the present
work have helped to select better purity components and improve shielding in
EDELWEISS-III to further reduce the expected rate of background events in the
next phase of the experiment.Comment: 15 pages, 9 figures, to be published in Astroparticle Physic
Bistability in the actin cortex
Multi-color fluorescence imaging experiments of wave forming Dictyostelium cells have revealed that actin waves separate two domains of the cell cortex that differ in their actin structure and phosphoinositide composition. We propose a bistable model of actin dynamics to account for these experimental observation. The model is based on the simplifying assumption that the actin cytoskeleton is composed of two distinct network types, a dendritic and a bundled network. The two structurally different states that were observed in experiments correspond to the stable fixed points in the bistable regime of this model. Each fixed point is dominated by one of the two network types. The experimentally observed actin waves can be considered as trigger waves that propagate transitions between the two stable fixed points
Crystal Structure of HIV-1 gp41 Including Both Fusion Peptide and Membrane Proximal External Regions
The HIV-1 envelope glycoprotein (Env) composed of the receptor binding domain gp120 and the fusion protein subunit gp41 catalyzes virus entry and is a major target for therapeutic intervention and for neutralizing antibodies. Env interactions with cellular receptors trigger refolding of gp41, which induces close apposition of viral and cellular membranes leading to membrane fusion. The energy released during refolding is used to overcome the kinetic barrier and drives the fusion reaction. Here, we report the crystal structure at 2 Å resolution of the complete extracellular domain of gp41 lacking the fusion peptide and the cystein-linked loop. Both the fusion peptide proximal region (FPPR) and the membrane proximal external region (MPER) form helical extensions from the gp41 six-helical bundle core structure. The lack of regular coiled-coil interactions within FPPR and MPER splay this end of the structure apart while positioning the fusion peptide towards the outside of the six-helical bundle and exposing conserved hydrophobic MPER residues. Unexpectedly, the section of the MPER, which is juxtaposed to the transmembrane region (TMR), bends in a 90°-angle sideward positioning three aromatic side chains per monomer for membrane insertion. We calculate that this structural motif might facilitate the generation of membrane curvature on the viral membrane. The presence of FPPR and MPER increases the melting temperature of gp41 significantly in comparison to the core structure of gp41. Thus, our data indicate that the ordered assembly of FPPR and MPER beyond the core contributes energy to the membrane fusion reaction. Furthermore, we provide the first structural evidence that part of MPER will be membrane inserted within trimeric gp41. We propose that this framework has important implications for membrane bending on the viral membrane, which is required for fusion and could provide a platform for epitope and lipid bilayer recognition for broadly neutralizing gp41 antibodies
Production of phi mesons at mid-rapidity in sqrt(s_NN) = 200 GeV Au+Au collisions at RHIC
We present the first results of meson production in the K^+K^- decay channel
from Au+Au collisions at sqrt(s_NN) = 200 GeV as measured at mid-rapidity by
the PHENIX detector at RHIC. Precision resonance centroid and width values are
extracted as a function of collision centrality. No significant variation from
the PDG accepted values is observed. The transverse mass spectra are fitted
with a linear exponential function for which the derived inverse slope
parameter is seen to be constant as a function of centrality. These data are
also fitted by a hydrodynamic model with the result that the freeze-out
temperature and the expansion velocity values are consistent with the values
previously derived from fitting single hadron inclusive data. As a function of
transverse momentum the collisions scaled peripheral.to.central yield ratio RCP
for the is comparable to that of pions rather than that of protons. This result
lends support to theoretical models which distinguish between baryons and
mesons instead of particle mass for explaining the anomalous proton yield.Comment: 326 authors, 24 pages text, 23 figures, 6 tables, RevTeX 4. To be
submitted to Physical Review C as a regular article. Plain text data tables
for the points plotted in figures for this and previous PHENIX publications
are (or will be) publicly available at http://www.phenix.bnl.gov/papers.htm
Performance of the CMS Cathode Strip Chambers with Cosmic Rays
The Cathode Strip Chambers (CSCs) constitute the primary muon tracking device
in the CMS endcaps. Their performance has been evaluated using data taken
during a cosmic ray run in fall 2008. Measured noise levels are low, with the
number of noisy channels well below 1%. Coordinate resolution was measured for
all types of chambers, and fall in the range 47 microns to 243 microns. The
efficiencies for local charged track triggers, for hit and for segments
reconstruction were measured, and are above 99%. The timing resolution per
layer is approximately 5 ns
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