Comparison of neutron scattering and DFM capacitance instruments in measuring soil water evaporation

Soil water evaporation is an important parameter that needs to be accurately measured for the design of water-efficient agricultural systems. With this study, the abilities of the DFM capacitance probes and a neutron water meter (NWM) to measure evaporation from the soil surface were compared. Measured evaporation was compared to the control values measured with mini-lysimeters. Calibration of DFM capacitance probes and the NWM was done in the laboratory using the topsoil of a Bainsvlei soil form. Field measurements of soil water content were done on the same Bainsvlei soil. Calibration results indicated a good correspondence (r2 = 0.99) between the measured values and known volumetric soil water contents. There was no significant difference (p = 95%) between the DFM evaporation measurements and the control, whereas the NWM and control differed significantly. It was concluded that the DFM capacitance probe is a better tool than the NWM in measuring evaporation from the topsoil surface.Keywords: neutron water meter, capacitance probes, evaporation, soil wetnes

Review of ‘plant available water’ aspects of water use efficiency under irrigated and dryland conditions

This review provides an overview of Water Research Commission (WRC)-funded research over the past 36 years. A total of 28 WRC reports have been consulted, 13 of these compiled by the University of the Free State, 4 by the University of Fort Hare, and the remainder mainly by the ARC-Institute for Soil Climate and Water. This work has resulted in extensive capacity building in this field – numerous technical assistants and 58 researchers have been involved, of which 23 are still active in research.The focus on the water flow processes in the soil-plant-atmosphere continuum (SPAC), with particular emphasis on processes in the soil, has greatly enhanced understanding of the system, thereby enabling the formulation of a quantitative model relating the water supply from a layered soil profile to water demand; the formulation of logical quantitative definitions for crop-ecotope specific upper and lower limits of available water; the identification of the harmful rootzone development effects of compacted layers in fine sandy soils caused by cultivation, and amelioration procedures to prevent these effects; and management strategies to combat excessive water losses by deep drainage. The explanation of the way in which SPAC is expressed in the landscape in the form of the ecotope has been beneficial with regard to the extrapolation of studies on particular SPACs to the large number of ecotopes where detailed studies have not been possible. Valuable results are reported regarding rainfall and runoff management strategies. Longer fallow periods and deficit irrigation on certain crop ecotopes improved rainfall use efficiency. On semi-arid ecotopes with high-drought-risk clay and duplex soils and high runoff losses, in-field rainwater harvesting (IRWH), designed specifically for subsistence farmers, resulted in maize and sunflower yield increases of between 30% and 50% compared to yields obtained with conventional tillage. An indication of the level of understanding of the relevant processes that has been achieved is demonstrated by their quantitative description in mathematical and empirical models: BEWAB for irrigation, SWAMP mainly for dryland cropping, and CYP-SA for IRWH. Five important related research and development needs are identified. The WRC has played, and continues to play, an important role in commissioning and funding research on water utilisation in agriculture and has clearly made an excellent contribution to the progress made in addressing the needs and requirements of subsistence, emergent and dryland farmers in South Africa.Keywords: BEWAB, SWAMP, CYP-SA, in-field rainwater harvesting, dryland ecotope, irrigatio

Investigating the biological properties of carbohydrate derived fulvic acid (CHD-FA) as a potential novel therapy for the management of oral biofilm infections.

Background: A number of oral diseases, including periodontitis, derive from microbial biofilms and are associated with increased antimicrobial resistance. Despite the widespread use of mouthwashes being used as adjunctive measures to control these biofilms, their prolonged use is not recommended due to various side effects. Therefore, alternative broad-spectrum antimicrobials that minimise these effects are highly sought after. Carbohydrate derived fulvic acid (CHD-FA) is an organic acid which has previously demonstrated to be microbiocidal against Candida albicans biofilms, therefore, the aims of this study were to evaluate the antibacterial activity of CHD-FA against orally derived biofilms and to investigate adjunctive biological effects.<p></p> Methods: Minimum inhibitory concentrations were evaluated for CHD-FA and chlorhexidine (CHX) against a range of oral bacteria using standardised microdilution testing for planktonic and sessile. Scanning electron microscopy was also employed to visualise changes in oral biofilms after antimicrobial treatment. Cytotoxicity of these compounds was assessed against oral epithelial cells, and the effect of CHD-FA on host inflammatory markers was assessed by measuring mRNA and protein expression.<p></p> Results: CHD-FA was highly active against all of the oral bacteria tested, including Porphyromonas gingivalis, with a sessile minimum inhibitory concentration of 0.5%. This concentration was shown to kill multi-species biofilms by approximately 90%, levels comparable to that of chlorhexidine (CHX). In a mammalian cell culture model, pretreatment of epithelial cells with buffered CHD-FA was shown to significantly down-regulate key inflammatory mediators, including interleukin-8 (IL-8), after stimulation with a multi-species biofilm.<p></p> Conclusions: Overall, CHD-FA was shown to possess broad-spectrum antibacterial activity, with a supplementary function of being able to down-regulate inflammation. These properties offer an attractive spectrum of function from a naturally derived compound, which could be used as an alternative topical treatment strategy for oral biofilm diseases. Further studies in vitro and in vivo are required to determine the precise mechanism by which CHD-FA modulates the host immune response.<p></p&gt

The Present and Future Role of Insect-Resistant Genetically Modified Maize in IPM

Commercial, genetically-modified (GM) maize was first planted in the United States (USA, 1996) and Canada (1997) but now is grown in 13 countries on a total of over 35 million hectares (\u3e24% of area worldwide). The first GM maize plants produced a Cry protein derived from the soil bacteriumBacillus thuringiensis (Bt), which made them resistant to European corn borer and other lepidopteran maize pests. New GM maize hybrids not only have resistance to lepidopteran pests but some have resistance to coleopteran pests and tolerance to specific herbicides. Growers are attracted to the Btmaize hybrids for their convenience and because of yield protection, reduced need for chemical insecticides, and improved grain quality. Yet, most growers worldwide still rely on traditional integrated pest management (IPM) methods to control maize pests. They must weigh the appeal of buying insect protection “in the bag” against questions regarding economics, environmental safety, and insect resistance management (IRM). Traditional management of maize insects and the opportunities and challenges presented by GM maize are considered as they relate to current and future insect-resistant products. Four countries, two that currently have commercialize Bt maize (USA and Spain) and two that do not (China and Kenya), are highlighted. As with other insect management tactics (e.g., insecticide use or tillage), GM maize should not be considered inherently compatible or incompatible with IPM. Rather, the effect of GM insect-resistance on maize IPM likely depends on how the technology is developed and used

Genome-wide association and transcriptome studies identify target genes and risk loci for breast cancer

Genome-wide association studies (GWAS) have identified more than 170 breast cancer susceptibility loci. Here we hypothesize that some risk-associated variants might act in non-breast tissues, specifically adipose tissue and immune cells from blood and spleen. Using expression quantitative trait loci (eQTL) reported in these tissues, we identify 26 previously unreported, likely target genes of overall breast cancer risk variants, and 17 for estrogen receptor (ER)-negative breast cancer, several with a known immune function. We determine the directional effect of gene expression on disease risk measured based on single and multiple eQTL. In addition, using a gene-based test of association that considers eQTL from multiple tissues, we identify seven (and four) regions with variants associated with overall (and ER-negative) breast cancer risk, which were not reported in previous GWAS. Further investigation of the function of the implicated genes in breast and immune cells may provide insights into the etiology of breast cancer.Peer reviewe

Action to protect the independence and integrity of global health research

Storeng KT, Abimbola S, Balabanova D, et al. Action to protect the independence and integrity of global health research. BMJ GLOBAL HEALTH. 2019;4(3): e001746