Crimes of Obedience: Toward Social Psychology of Authority and Responsibility

A Review of Crimes of Obedience: Toward Social Psychology of Authority and Responsibility by Herbert C. Kelman and V. Lee Hamilto

Elastic biodegradable starch/ethylene-co-vinyl alcohol fibre-mesh scaffolds for tissue engineering applications

The fabrication of a biomaterial scaffold, with adequate physical and structural properties for tissue engineering applications, is reported. A blend of starch with ethylene-vinyl alcohol (50/50 w/w, SEVA-C) is used to produce 3D fibre-mesh scaffolds by wet-spinning. The scaffolds are characterized in terms of morphology, porosity, interconnectivity, and pore size, using scanning electron microscopy (SEM) and microcomputed tomography (μCT). The degradation behavior, as well as the mechanical properties of the scaffolds, is investigated in presence of alpha-amylase enzyme at physiological concentration. Scaffolds with porosities ranging from 43 to 52%, interconnectivity of ∼70.5% and pore size between 118 and 159 μm, can be fabricated using the proposed methodology. The scaffolds exhibit an elastic behavior in the wet state with a compressive modulus of 7.96±0.32 MPa. Degradation studies show that SEVA-C scaffolds are susceptible to enzymatic degradation by alpha-amylase, confirmed by the increase of weight loss (40% of weight loss after 12 weeks) and presence of degradation products (reducing sugars) in solution. The diameter of SEVA-C scaffolds decreases with degradation time, increasing the overall porosity, interconnectivity and pore size. In vitro cell studies with human osteosarcoma cell line (SaOs-2) showed a nontoxic and cytocompatible behavior of the developed fibre mesh scaffolds. The positive cellular response, together with structural and degradable properties, suggests that 3D SEVA-C fibre-meshes may be good candidates as tissue engineering scaffolds. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40504. Copyright © 2014 Wiley Periodicals, Inc.This work was supported by national funds through the Portuguese Foundation for Science and Technology under the scope of the project PTDC/CTM/67560/2006 and by the European Regional Development Fund (ERDF) through the Operational Competitiveness Programme “COMPETE” (FCOMP-01-0124-FEDER-007148)

Paying for performance : an analysis of output-based aid in education

Over the last decade, growing attention has been paid to emerging mechanisms for results-based financing (RBF) that seek to address the perceived shortcomings of traditional development aid and financing and improve its effectiveness through placing an emphasis on results. In 2003 the World Bank introduced the concept of output-based aid (OBA) through the Global Partnership on OBA (GPOBA) that now includes a consortium of international multilateral and bilateral donors, and in 2012 the World Bank developed a new RBF tool known as program-for-results (PforR). With OBA, service delivery is typically contracted out by the entity providing the public funds to a service provider (for example, a private enterprise, public utility, non-government organization (NGO), or community-based organization), and payments are linked to the achievement of pre-defined service performance or outputs (GPOBA). Mechanisms through which OBA may lead to improved results include: increased transparency (though explicitly linking subsidies to targets), increased accountability of service providers (as funds are disbursed only upon reaching targets), greater private sector engagement, greater opportunities for the service provider to be innovative and efficient (given autonomy provided to service providers), and improvements in monitoring systems. In 2014-2015, results for development institute (R4D) conducted a scoping study on the potential for OBA in education for GPOBA. This report provides the consolidated findings from the study which included: (i) a literature review of RBF schemes in education, (ii) a landscaping and analysis of existing OBA projects in education, and (iii) recommendations for applying OBA in the education sector moving forward

Differential effects of five types of antipathogenic plant peptides on model membranes

he effects of five antipathogenic plant peptides, wheat α-thionin, potato PTH1 defensin, barley LTP2 lipid transfer protein, and potato tuber DL1 and DL2 defensins, have been tested against phospholipid vesicles (liposomes). Wheat thionin very actively induces aggregation and leakage of negatively charged vesicles. LTP2 displays the same activities, although to a limited extent. Under certain conditions PTH1 and DL2 induce vesicle aggregation, but not leakage. Potato defensin DL1 failed to show any effect on liposomes. The same peptides have been assayed against a plant pathogenic bacterium, both the membrane-active and -inactive compounds having efficient antibacterial actio

The Solution Structures of Two Human IgG1 Antibodies Show Conformational Stability and Accommodate Their C1q and FcγR Ligands.

The human IgG1 antibody subclass shows distinct properties compared with the IgG2, IgG3, and IgG4 subclasses and is the most exploited subclass in therapeutic antibodies. It is the most abundant subclass, has a half-life as long as that of IgG2 and IgG4, binds the FcγR receptor, and activates complement. There is limited structural information on full-length human IgG1 because of the challenges of crystallization. To rectify this, we have studied the solution structures of two human IgG1 6a and 19a monoclonal antibodies in different buffers at different temperatures. Analytical ultracentrifugation showed that both antibodies were predominantly monomeric, with sedimentation coefficients s20,w (0) of 6.3-6.4 S. Only a minor dimer peak was observed, and the amount was not dependent on buffer conditions. Solution scattering showed that the x-ray radius of gyration Rg increased with salt concentration, whereas the neutron Rg values remained unchanged with temperature. The x-ray and neutron distance distribution curves P(r) revealed two peaks, M1 and M2, whose positions were unchanged in different buffers to indicate conformational stability. Constrained atomistic scattering modeling revealed predominantly asymmetric solution structures for both antibodies with extended hinge structures. Both structures were similar to the only known crystal structure of full-length human IgG1. The Fab conformations in both structures were suitably positioned to permit the Fc region to bind readily to its FcγR and C1q ligands without steric clashes, unlike human IgG4. Our molecular models for human IgG1 explain its immune activities, and we discuss its stability and function for therapeutic applications

Structure of complement C3(H2O) revealed by quantitative cross-linking/mass spectrometry and modelling:QCLMS and modelling reveals structure of C3(H2O)

The slow but spontaneous and ubiquitous formation of C3(H(2)O), the hydrolytic and conformationally rearranged product of C3, initiates antibody-independent activation of the complement system that is a key first line of antimicrobial defense. The structure of C3(H(2)O) has not been determined. Here we subjected C3(H(2)O) to quantitative cross-linking/mass spectrometry (QCLMS). This revealed details of the structural differences and similarities between C3(H(2)O) and C3, as well as between C3(H(2)O) and its pivotal proteolytic cleavage product, C3b, which shares functionally similarity with C3(H(2)O). Considered in combination with the crystal structures of C3 and C3b, the QCMLS data suggest that C3(H(2)O) generation is accompanied by the migration of the thioester-containing domain of C3 from one end of the molecule to the other. This creates a stable C3b-like platform able to bind the zymogen, factor B, or the regulator, factor H. Integration of available crystallographic and QCLMS data allowed the determination of a 3D model of the C3(H(2)O) domain architecture. The unique arrangement of domains thus observed in C3(H(2)O), which retains the anaphylatoxin domain (that is excised when C3 is enzymatically activated to C3b), can be used to rationalize observed differences between C3(H(2)O) and C3b in terms of complement activation and regulation

Aid and Poverty

SUMMARY The volume of aid received by the less developed countries, with some exceptions, is small in relation to the existing scale of poverty in those countries. Aid makes a significant but incremental contribution to available national resources. What aid can or cannot do for the poor has to be viewed and assessed within the context of overall national policies and priorities towards poverty elimination. In that context, it can be said that aid has made a smaller impact on poverty than one would expect. The reasons for this are varied and complex, and point to shortcomings in both donor and recipient policies. The aid experience, however, is by no means wholly negative. Particular forms of aid can make a positive contribution to a reduction in poverty, where national policies are directed towards the same objectives. More important for the future, perhaps, are the lessons that can be learnt from the aid experience about the do's and don'ts of aid policy. SOMMAIRE L'aide et la pauvreté Le volume de l'aide reçu par les pays moins développés, avec quelques exceptions, est petit en rapport avec la pauvreté dans ces pays. L'aide contribue substantiellement mais d'une manière incrémentale aux resources nationales disponibles de ces pays. Le potentiel de l'aide doit être évalué dans le contexte globale des politiques nationales et des priorités envers l'élimination de la pauvreté. Dans ce contexte, on peut dire que l'influence de l'aide sur la pauvreté a été plus petite de ce qu'on aurait prévu. Les raisons pour ceci sont diverses et complexes, et soulignent des défauts dans les politiques des donneurs, aussi bien que dans celles des pays recevants. Cependant, l'expérience de l'aide n'est d'aucune manière entièrement négative. Certaines formes d'aide peuvent réduire la pauvreté, lá où les politiques nationales sont formulées dans le même but. Plus important pour l'avenir, peut?être, sont les leçons de l'expérience de l'aide en ce qui concerne ce qu'on peut faire, et ce qu'on ne devrait pas faire en matière de politique de l'aide. RESUMEN Ayuda y Pobreza Con algunas excepciones, el volumen de la ayuda recibida por los países menos desarrollados es pequeño en relación a la escala de pobreza existente en esos países. La ayuda implica una contribución significativa y adicional a los recursos nacionales existentes. Lo que la ayuda puede y no puede hacer en favor de los pobres, debe considerarse y evaluarse en el contexto del conjunto de las políticas y prioridades nacionales de erradicación de la pobreza. En este contexto puede decirse que el impacto de la ayuda sobre la pobreza, es menor del que podría esperarse. Las razones son variadas y complejas y se refieren a deficiencias en las políticas de donantes y receptores. No obstante, la experiencia en ayuda está lejos de ser totalmente negativa. Determinadas formas de ayuda pueden realizar una contribución positiva en la reducción de la pobreza, en los casos en que las políticas nacionales están dirigidas a los mismos objetivos. Probablemente lo más importante para el futuro, son las lecciones que la experiencia en ayuda proporciona acerca de lo que debería y no debería involucrar la política de ayuda

A New Approach to the Allocation of Aid among Developing Countries: Is the USA Different from the Rest?

This paper attempts to explain the factors that determine the geographical allocation of foreign aid. Its novelty is that it develops a rigorous theoretical model and conducts the corresponding empirical investigations based on a large panel dataset. We run regressions for different major donors (United States, Canada, France, Italy, Japan, United Kingdom, and multilateral organizations) with the explicit objective of establishing whether the United States, in light of its geopolitical hegemony, behaves differently from others. We find that all the donors respond to recipient need in their allocation of aid, but that the United States puts less emphasis on this than the other donors with the exception of Japan. We also find that the United States puts more emphasis on donor–recipient linkages than do the other donors suggesting that the United States attaches greater importance to issues of donor interest, for example, geopolitical, commercial, and other links with specific recipients