Histopathological and ultrastructural aspects of mice lungs experimentally infected with dengue virus serotype 2

Submitted by Sandra Infurna ([email protected]) on 2020-03-10T12:29:17Z No. of bitstreams: 1 OrtrudBarth_DebraBarreto_etal_IOC_2007.pdf: 6204757 bytes, checksum: 46827ce79812b88ea66bc558554d3d3a (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2020-03-10T12:35:33Z (GMT) No. of bitstreams: 1 OrtrudBarth_DebraBarreto_etal_IOC_2007.pdf: 6204757 bytes, checksum: 46827ce79812b88ea66bc558554d3d3a (MD5)Made available in DSpace on 2020-03-10T12:35:33Z (GMT). No. of bitstreams: 1 OrtrudBarth_DebraBarreto_etal_IOC_2007.pdf: 6204757 bytes, checksum: 46827ce79812b88ea66bc558554d3d3a (MD5) Previous issue date: 2007Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Histological and ultrastructural alterations in lung tissue of BALB/c mice infected with dengue virus serotype 2 (non-neuroadapted), by intraperitoneal and intravenous routes were analyzed. Lung tissues were processed following the standard techniques for photonic and electron transmission microscopies. Histopathological and ultrastructural studies showed interstitial pneumonia, characterized by the presence of mononuclear cells. In the mouse model, the dengue virus serotype 2 seems to led to a transient inflammatory process without extensive damage to the interalveolar septa, but caused focal alterations of the blood-exchange barrier. Endothelial cells of blood capillaries exhibited phyllopodia suggesting activation by presence of dengue virus. Morphometrical analysis of mast cells showed an expressive increase of the number of these cells in peribronchiolar spaces and adjacent areas to the interalveolar septa. Alveolar macrophages showed particles dengue virus-like inside rough endoplasmic reticulum and Golgi complex, suggesting viral replication. The tissue alterations observed in our experimental model were similar to the observed in human cases of dengue fever and dengue hemorrhagic fever. Our results show that BALB/c mice are permissive host for dengue virus serotype 2 replication and therefore provides an useful model to study of morphological aspects of dengue virus infection

Two Lineages of Dengue Virus Type 2, Brazil

Our results suggest the circulation of genetically different DENV-2 in Brazil and that these viruses may have a role in severity of dengue diseases. These fi ndings can help to further understand the complex dynamic pathogenic profi le of dengue viruses and their circulation in dengue-endemic regions.Submitted by Sandra Infurna ([email protected]) on 2017-10-19T12:29:42Z No. of bitstreams: 1 jmg_araujo_etal_IOC_2010.pdf: 90948 bytes, checksum: becf560ca2fc3c395d59080bbdfcaf2c (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-10-19T12:45:14Z (GMT) No. of bitstreams: 1 jmg_araujo_etal_IOC_2010.pdf: 90948 bytes, checksum: becf560ca2fc3c395d59080bbdfcaf2c (MD5)Made available in DSpace on 2017-10-19T12:45:14Z (GMT). No. of bitstreams: 1 jmg_araujo_etal_IOC_2010.pdf: 90948 bytes, checksum: becf560ca2fc3c395d59080bbdfcaf2c (MD5) Previous issue date: 2010Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, BrasilUniversidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil

Borboletas da Mata Atlântica do Estado do Rio de Janeiro: Pieridae (Lepidoptera)

Forty seven taxa of Pieridae (46 species) are recorded from the state of Rio de Janeiro, including their municipalities of occurrence. These records come from papers containing lists of species, entomological collections, unpublished field surveys and personal observations of the authors.São apresentados quarenta e sete táxons (46 espécies) de Pieridae (Lepidoptera) registrados para o Estado do Rio de Janeiro, com seus municípios de ocorrência. O trabalho teve como base publicações de listas de espécies, dados de coleções entomológicas, levantamentos de campo não publicados e observações pessoais dos autores

Long-term thermal sensitivity of Earth’s tropical forests

The sensitivity of tropical forest carbon to climate is a key uncertainty in predicting global climate change. Although short-term drying and warming are known to affect forests, it is unknown if such effects translate into long-term responses. Here, we analyze 590 permanent plots measured across the tropics to derive the equilibrium climate controls on forest carbon. Maximum temperature is the most important predictor of aboveground biomass (−9.1 megagrams of carbon per hectare per degree Celsius), primarily by reducing woody productivity, and has a greater impact per °C in the hottest forests (>32.2°C). Our results nevertheless reveal greater thermal resilience than observations of short-term variation imply. To realize the long-term climate adaptation potential of tropical forests requires both protecting them and stabilizing Earth’s climate

Combined fit to the spectrum and composition data measured by the Pierre Auger Observatory including magnetic horizon effects

The measurements by the Pierre Auger Observatory of the energy spectrum and mass composition of cosmic rays can be interpreted assuming the presence of two extragalactic source populations, one dominating the flux at energies above a few EeV and the other below. To fit the data ignoring magnetic field effects, the high-energy population needs to accelerate a mixture of nuclei with very hard spectra, at odds with the approximate E$^{-2}$ shape expected from diffusive shock acceleration. The presence of turbulent extragalactic magnetic fields in the region between the closest sources and the Earth can significantly modify the observed CR spectrum with respect to that emitted by the sources, reducing the flux of low-rigidity particles that reach the Earth. We here take into account this magnetic horizon effect in the combined fit of the spectrum and shower depth distributions, exploring the possibility that a spectrum for the high-energy population sources with a shape closer to E$^{-2}$ be able to explain the observations

Studies of the mass composition of cosmic rays and proton-proton interaction cross-sections at ultra-high energies with the Pierre Auger Observatory

In this work, we present an estimate of the cosmic-ray mass composition from the distributions of the depth of the shower maximum (Xmax) measured by the fluorescence detector of the Pierre Auger Observatory. We discuss the sensitivity of the mass composition measurements to the uncertainties in the properties of the hadronic interactions, particularly in the predictions of the particle interaction cross-sections. For this purpose, we adjust the fractions of cosmic-ray mass groups to fit the data with Xmax distributions from air shower simulations. We modify the proton-proton cross-sections at ultra-high energies, and the corresponding air shower simulations with rescaled nucleus-air cross-sections are obtained via Glauber theory. We compare the energy-dependent composition of ultra-high-energy cosmic rays obtained for the different extrapolations of the proton-proton cross-sections from low-energy accelerator data