Application of the wavelet transform to biomedical signals

The basic concepts and fundamentals of the wavelet signal representation were examined. The orthonormal wavelet was selected for this project after being compared to various types of wavelets. The orthonormal wavelet was chosen due to the equal time and frequency resolution exhibited in the wavelet coefficients. Programs were written in Matlab to implement the orthonormal wavelet in developing wavelet coefficients for a given signal. The programs include the conditions for an orthonormal wavelet in and which produce the wavelet filters g(n) and h(n). The wavelet filters were then incorporated into another program that applied Mallat\u27s multiresolution algorithm for a given signal. The resulting wavelet coefficients were obtained and interpreted. The orthonormal wavelet was applied to various types of biomedical signals. The wavelet transform was applied to motor evoked potentials (MEPs) created cortical magnetic stimulation. The wavelet was also applied to evoked potentials (EPs) and to various types of EKG signals. The wavelet representation exposed new ways of observing biomedical signals by bringing out details and structures not present in the original waveforms

Profound Re-Organization of Cell Surface Proteome in Equine Retinal Pigment Epithelial Cells in Response to In Vitro Culturing

The purpose of this study was to characterize the cell surface proteome of native compared to cultured equine retinal pigment epithelium (RPE) cells. The RPE plays an essential role in visual function and represents the outer blood-retinal barrier. We are investigating immunopathomechanisms of equine recurrent uveitis, an autoimmune inflammatory disease in horses leading to breakdown of the outer blood-retinal barrier and influx of autoreactive T-cells into affected horses' vitrei. Cell surface proteins of native and cultured RPE cells from eye-healthy horses were captured by biotinylation, analyzed by high resolution mass spectrometry coupled to liquid chromatography (LC MS/MS), and the most interesting candidates were validated by PCR, immunoblotting and immunocytochemistry. A total of 112 proteins were identified, of which 84% were cell surface membrane proteins. Twenty-three of these proteins were concurrently expressed by both cell states, 28 proteins exclusively by native RPE cells. Among the latter were two RPE markers with highly specialized RPE functions: cellular retinaldehyde-binding protein (CRALBP) and retinal pigment epithelium-specific protein 65kDa (RPE65). Furthermore, 61 proteins were only expressed by cultured RPE cells and absent in native cells. As we believe that initiating events, leading to the breakdown of the outer blood-retinal barrier, take place at the cell surface of RPE cells as a particularly exposed barrier structure, this differential characterization of cell surface proteomes of native and cultured equine RPE cells is a prerequisite for future studies

A multi-decade record of high quality fCO2 data in version 3 of the Surface Ocean CO2 Atlas (SOCAT)

The Surface Ocean CO2 Atlas (SOCAT) is a synthesis of quality-controlled fCO2 (fugacity of carbon dioxide) values for the global surface oceans and coastal seas with regular updates. Version 3 of SOCAT has 14.7 million fCO2 values from 3646 data sets covering the years 1957 to 2014. This latest version has an additional 4.6 million fCO2 values relative to version 2 and extends the record from 2011 to 2014. Version 3 also significantly increases the data availability for 2005 to 2013. SOCAT has an average of approximately 1.2 million surface water fCO2 values per year for the years 2006 to 2012. Quality and documentation of the data has improved. A new feature is the data set quality control (QC) flag of E for data from alternative sensors and platforms. The accuracy of surface water fCO2 has been defined for all data set QC flags. Automated range checking has been carried out for all data sets during their upload into SOCAT. The upgrade of the interactive Data Set Viewer (previously known as the Cruise Data Viewer) allows better interrogation of the SOCAT data collection and rapid creation of high-quality figures for scientific presentations. Automated data upload has been launched for version 4 and will enable more frequent SOCAT releases in the future. High-profile scientific applications of SOCAT include quantification of the ocean sink for atmospheric carbon dioxide and its long-term variation, detection of ocean acidification, as well as evaluation of coupled-climate and ocean-only biogeochemical models. Users of SOCAT data products are urged to acknowledge the contribution of data providers, as stated in the SOCAT Fair Data Use Statement. This ESSD (Earth System Science Data) “living data” publication documents the methods and data sets used for the assembly of this new version of the SOCAT data collection and compares these with those used for earlier versions of the data collection (Pfeil et al., 2013; Sabine et al., 2013; Bakker et al., 2014). Individual data set files, included in the synthesis product, can be downloaded here: doi:10.1594/PANGAEA.849770. The gridded products are available here: doi:10.3334/CDIAC/OTG.SOCAT_V3_GRID

Genome-Wide Analysis of Müller Glial Differentiation Reveals a Requirement for Notch Signaling in Postmitotic Cells to Maintain the Glial Fate

Previous studies have shown that Müller glia are closely related to retinal progenitors; these two cell types express many of the same genes and after damage to the retina, Müller glia can serve as a source for new neurons, particularly in non-mammalian vertebrates. We investigated the period of postnatal retinal development when progenitors are differentiating into Müller glia to better understand this transition. FACS purified retinal progenitors and Müller glia from various ages of Hes5-GFP mice were analyzed by Affymetrix cDNA microarrays. We found that genes known to be enriched/expressed by Müller glia steadily increase over the first three postnatal weeks, while genes associated with the mitotic cell cycle are rapidly downregulated from P0 to P7. Interestingly, progenitor genes not directly associated with the mitotic cell cycle, like the proneural genes Ascl1 and Neurog2, decline more slowly over the first 10–14 days of postnatal development, and there is a peak in Notch signaling several days after the presumptive Müller glia have been generated. To confirm that Notch signaling continues in the postmitotic Müller glia, we performed in situ hybridization, immunolocalization for the active form of Notch, and immunofluorescence for BrdU. Using genetic and pharmacological approaches, we found that sustained Notch signaling in the postmitotic Müller glia is necessary for their maturation and the stabilization of the glial identity for almost a week after the cells have exited the mitotic cell cycle

The Role of Mislocalized Phototransduction in Photoreceptor Cell Death of Retinitis Pigmentosa

Most of inherited retinal diseases such as retinitis pigmentosa (RP) cause photoreceptor cell death resulting in blindness. RP is a large family of diseases in which the photoreceptor cell death can be caused by a number of pathways. Among them, light exposure has been reported to induce photoreceptor cell death. However, the detailed mechanism by which photoreceptor cell death is caused by light exposure is unclear. In this study, we have shown that even a mild light exposure can induce ectopic phototransduction and result in the acceleration of rod photoreceptor cell death in some vertebrate models. In ovl, a zebrafish model of outer segment deficiency, photoreceptor cell death is associated with light exposure. The ovl larvae show ectopic accumulation of rhodopsin and knockdown of ectopic rhodopsin and transducin rescue rod photoreceptor cell death. However, knockdown of phosphodiesterase, the enzyme that mediates the next step of phototransduction, does not. So, ectopic phototransduction activated by light exposure, which leads to rod photoreceptor cell death, is through the action of transducin. Furthermore, we have demonstrated that forced activation of adenylyl cyclase in the inner segment leads to rod photoreceptor cell death. For further confirmation, we have also generated a transgenic fish which possesses a human rhodopsin mutation, Q344X. This fish and rd10 model mice show photoreceptor cell death caused by adenylyl cyclase. In short, our study indicates that in some RP, adenylyl cyclase is involved in photoreceptor cell death pathway; its inhibition is potentially a logical approach for a novel RP therapy

Functional insights into the infective larval stage of Anisakis simplex s.s., Anisakis pegreffii and their hybrids based on gene expression patterns

List of species and specimen used in the phylogenetic tree of Additional file 1. Code of the voucher specimen and accession number for mitochondrial gene COII (*: sequences obtained from GenBank). Labeled are the specimens selected for RNA sequencing (first number, population; second number specimen). A. simplex s.s. – A. pegreffii refers to hybrids haplotype according Abollo et al. [23]. (DOCX 47 kb

Formes de parenté artificielle dans le Haut Moyen-Age

Dans une esquisse qui commence avec la période préchrétienne et se prolonge jusqu'au IXe siècle, sont discutées les formes exemplaires de parenté artificielle auxquelles on a eu recours, dans l'Antiquité tardive et le Haut Moyen Age, pour étendre et modifier les relations de parenté naturelle. Ce faisant, on tenait compte à la fois des phénomènes d'ordre «pneumatique », comme la parenté spirituelle et la cognatio spiritualis, et des relations politiques. Dans ce dernier cas, l'étude des titres protocollaires de parenté grâce auxquels les empereurs de Constantinople se sont mis à la tête de la famille mondiale des rois, ou celle de la compa- ternitas des papes qui devait renforcer l'alliance avec les Carolingiens à partir de 784, débouchent sur des résultats particulièrement significatifs.Hauck Karl. Formes de parenté artificielle dans le Haut Moyen-Age. In: Famille et parenté dans l'Occident médiéval. Actes du colloque de Paris (6-8 juin 1974) Rome : École Française de Rome, 1977. pp. 43-47. (Publications de l'École française de Rome, 30

Formes de parenté artificielle dans le Haut Moyen-Age

Dans une esquisse qui commence avec la période préchrétienne et se prolonge jusqu'au IXe siècle, sont discutées les formes exemplaires de parenté artificielle auxquelles on a eu recours, dans l'Antiquité tardive et le Haut Moyen Age, pour étendre et modifier les relations de parenté naturelle. Ce faisant, on tenait compte à la fois des phénomènes d'ordre «pneumatique », comme la parenté spirituelle et la cognatio spiritualis, et des relations politiques. Dans ce dernier cas, l'étude des titres protocollaires de parenté grâce auxquels les empereurs de Constantinople se sont mis à la tête de la famille mondiale des rois, ou celle de la compa- ternitas des papes qui devait renforcer l'alliance avec les Carolingiens à partir de 784, débouchent sur des résultats particulièrement significatifs.Hauck Karl. Formes de parenté artificielle dans le Haut Moyen-Age. In: Famille et parenté dans l'Occident médiéval. Actes du colloque de Paris (6-8 juin 1974) Rome : École Française de Rome, 1977. pp. 43-47. (Publications de l'École française de Rome, 30