Anomalous morphology in left hemisphere motor and premotor cortex of children who stutter

Stuttering is a neurodevelopmental disorder that affects the smooth flow of speech production. Stuttering onset occurs during a dynamic period of development when children first start learning to formulate sentences. Although most children grow out of stuttering naturally, ∼1% of all children develop persistent stuttering that can lead to significant psychosocial consequences throughout one’s life. To date, few studies have examined neural bases of stuttering in children who stutter, and even fewer have examined the basis for natural recovery versus persistence of stuttering. Here we report the first study to conduct surface-based analysis of the brain morphometric measures in children who stutter. We used FreeSurfer to extract cortical size and shape measures from structural MRI scans collected from the initial year of a longitudinal study involving 70 children (36 stuttering, 34 controls) in the 3–10-year range. The stuttering group was further divided into two groups: persistent and recovered, based on their later longitudinal visits that allowed determination of their eventual clinical outcome. A region of interest analysis that focused on the left hemisphere speech network and a whole-brain exploratory analysis were conducted to examine group differences and group × age interaction effects. We found that the persistent group could be differentiated from the control and recovered groups by reduced cortical thickness in left motor and lateral premotor cortical regions. The recovered group showed an age-related decrease in local gyrification in the left medial premotor cortex (supplementary motor area and and pre-supplementary motor area). These results provide strong evidence of a primary deficit in the left hemisphere speech network, specifically involving lateral premotor cortex and primary motor cortex, in persistent developmental stuttering. Results further point to a possible compensatory mechanism involving left medial premotor cortex in those who recover from childhood stuttering.This study was supported by Award Numbers R01DC011277 (SC) and R01DC007683 (FG) from the National Institute on Deafness and other Communication Disorders (NIDCD). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIDCD or the National Institutes of Health. (R01DC011277 - National Institute on Deafness and other Communication Disorders (NIDCD); R01DC007683 - National Institute on Deafness and other Communication Disorders (NIDCD))Accepted manuscrip

Egg Yolk Pigmentation as Influenced by Alfalfa Meal and Various Concentrates

This study was a continuation of testing natural and synthetic materials in the production of deeply pigmented egg yolk. Thus far ß-apo 8\u27 carotenoic acid ethyl ester (BACE) has been the most efficient pigmenter used, followed by ß-apo 8\u27 carotenal (BAC) and then various alfalfa concentrates, alfalfa or corn gluten meal and marigold meals. There has been a tendency for enhanced yolk color when the synthetic pigmenters were fed with alfalfa, suggesting synergism. This study was therefore conducted to determine if fractions of alfalfa meal with little or no xanthophyll would enhance the utilization of pure pigmenting material

Selenium Supplementation of Layer Diets Based on Milo and Soybean Meal

Previous studies with com-soy diets have shown laying hens to have improved feed efficiency when the diets were supplemented with up to 2 ppm selenium. In some cases, egg production was Improved, but in most instances feed intake was simply reduced without causing a decrease in egg numbers. A study was therefore conducted with a milo-soy diet to further evaluate this treatment

The Intestinal Roundworm Ascaris suum Releases Antimicrobial Factors Which Interfere With Bacterial Growth and Biofilm Formation

Ascariasis is a widespread soil-transmitted helminth infection caused by the intestinal roundworm Ascaris lumbricoides in humans, and the closely related Ascaris suum in pigs. Progress has been made in understanding interactions between helminths and host immune cells, but less is known concerning the interactions of parasitic nematodes and the host microbiota. As the host microbiota represents the direct environment for intestinal helminths and thus a considerable challenge, we studied nematode products, including excretory-secretory products (ESP) and body fluid (BF), of A. suum to determine their antimicrobial activities. Antimicrobial activities against gram-positive and gram-negative bacterial strains were assessed by the radial diffusion assay, while effects on biofilm formation were assessed using the crystal violet static biofilm and macrocolony assays. In addition, bacterial neutralizing activity was studied by an agglutination assay. ESP from different A. suum life stages (in vitro-hatched L3, lung-stage L3, L4, and adult) as well as BF from adult males were analyzed by mass spectrometry. Several proteins and peptides with known and predicted roles in nematode immune defense were detected in ESP and BF samples, including members of A. suum antibacterial factors (ASABF) and cecropin antimicrobial peptide families, glycosyl hydrolase enzymes such as lysozyme, as well as c-type lectin domain-containing proteins. Native, unconcentrated nematode products from intestine-dwelling L4-stage larvae and adults displayed broad-spectrum antibacterial activity. Additionally, adult A. suum ESP interfered with biofilm formation by Escherichia coli, and caused bacterial agglutination. These results indicate that A. suum uses a variety of factors with broad-spectrum antibacterial activity to affirm itself within its microbe-rich environment in the gut

Global transcript levels respond to small changes of the carbon status during progressive exhaustion of carbohydrates in Arabidopsis rosettes

The balance between the supply and utilization of carbon (C) changes continually. It has been proposed that plants respond in an acclimatory manner, modifying C utilization to minimize harmful periods of C depletion. This hypothesis predicts that signaling events are initiated by small changes in C status. We analyzed the global transcriptional response to a gradual depletion of C during the night and an extension of the night, where C becomes severely limiting from 4 h onward. The response was interpreted using published datasets for sugar, light, and circadian responses. Hundreds of C-responsive genes respond during the night and others very early in the extended night. Pathway analysis reveals that biosynthesis and cellular growth genes are repressed during the night and genes involved in catabolism are induced during the first hours of the extended night. The C response is amplified by an antagonistic interaction with the clock. Light signaling is attenuated during the 24-h light/dark cycle. A model was developed that uses the response of 22K genes during a circadian cycle and their responses to C and light to predict global transcriptional responses during diurnal cycles of wild-type and starchless pgm mutant plants and an extended night in wild-type plants. By identifying sets of genes that respond at different speeds and times during C depletion, our extended dataset and model aid the analysis of candidates for C signaling. This is illustrated for AKIN10 and four bZIP transcription factors, and sets of genes involved in trehalose signaling, protein turnover, and starch breakdown

Low abundances of heavy elements in the solar outer layers: comparisons of solar models with helioseismic inversions

Recent solar photospheric abundance analyses have led to a significant reduction of the metal abundances compared to the previous determinations. The solar models computed with standard opacities and diffusion processes using these new abundances give poor agreement with helioseismic inversions for the sound-speed profile, the surface helium abundance, and the convective zone depth. We attempt to obtain a good agreement between helioseismic inversions and solar models which present the "old" mixture in the interior and new chemical composition in the convective zone. To reach this result, we assume an undermetallic accretion at the beginning of the main sequence. We compute solar models with the Toulouse-Geneva Evolution Code, in which we simulate an undermetallic accretion in the early stages of the main sequence, in order to obtain new mixture in the outer convective zone. We compare the sound-speed profile, the convective zone depth, and the surface helium abundance with those deduced from helioseismology. The model with accretion but without any mixing process inside is in better agreement with helioseismology than the solar model with the new abundances throughout. There is, however, a spike under the convective zone which reaches 3.4%. Furthermore, the convective zone depth and the surface helium abundance are too low. Introducing undershooting below the convective zone allows us to recover the good convective zone radius and the addition of rotation-induced mixing and tachocline allows us to reconcile the surface helium abundance. But in any case the agreement of the sound-speed profile with helioseismic inference is worse than obtained with the old abundances.Comment: 5 pages, 2 figure

FUEL PERFORMANCE IMPROVEMENT PROGRAM Power-Ramp Testing and Postirradiation Examination of PCI- Resistant LWR Fuel Rod Designs

This report describes the power-ramp testing results from 10 fuel rods irradiated in the Halden Boiling Water Reactor (HBWR), Halden, Norway. Tne work is part of the Fuel Performance Improvement Program (FPIP), which is sponsored by the U.S. Department of Energy (DUE) and is conducted through the joint efforts of Consumers Power Company, Exxon Nuclear Company, lnc., and Pacific Northwest Laboratory. The objective of the FPlP is to identify and demonstrate fuel concepts with improved pellet-cladding interaction (PCl) behavior that will be capable of extended burnup. The postirradiation examination results obtained from one nonramped rod are also presented. The power-ramping behavior of three basic fuel rod types--rods with annular-pellet fuel, sphere-pac fuel, and dished-pellet (reference) fuel--are compared in terms of mechanisms known to promote PCl failures. The effects of graphite coating on the inside cladding surface and helium pressurization in rods witn annular fuel are also evaluated

Sugars and circadian regulation make major contributions to the global regulation of diurnal gene expression in Arabidopsis

The diurnal cycle strongly influences many plant metabolic and physiological processes. Arabidopsis thaliana rosettes were harvested six times during 12-h-light/12-h-dark treatments to investigate changes in gene expression using ATH1 arrays. Diagnostic gene sets were identified from published or in-house expression profiles of the response to light, sugar, nitrogen, and water deficit in seedlings and 4 h of darkness or illumination at ambient or compensation point [CO2]. Many sugar-responsive genes showed large diurnal expression changes, whose timing matched that of the diurnal changes of sugars. A set of circadian-regulated genes also showed large diurnal changes in expression. Comparison of published results from a free-running cycle with the diurnal changes in Columbia-0 (Col-0) and the starchless phosphoglucomutase (pgm) mutant indicated that sugars modify the expression of up to half of the clock-regulated genes. Principle component analysis identified genes that make large contributions to diurnal changes and confirmed that sugar and circadian regulation are the major inputs in Col-0 but that sugars dominate the response in pgm. Most of the changes in pgm are triggered by low sugar levels during the night rather than high levels in the light, highlighting the importance of responses to low sugar in diurnal gene regulation. We identified a set of candidate regulatory genes that show robust responses to alterations in sugar levels and change markedly during the diurnal cycle

Can a “state of the art” chemistry transport model simulate Amazonian tropospheric chemistry?

We present an evaluation of a nested high-resolution Goddard Earth Observing System (GEOS)-Chem chemistry transport model simulation of tropospheric chemistry over tropical South America. The model has been constrained with two isoprene emission inventories: (1) the canopy-scale Model of Emissions of Gases and Aerosols from Nature (MEGAN) and (2) a leaf-scale algorithm coupled to the Lund-Potsdam-Jena General Ecosystem Simulator (LPJ-GUESS) dynamic vegetation model, and the model has been run using two different chemical mechanisms that contain alternative treatments of isoprene photo-oxidation. Large differences of up to 100 Tg C yr^(−1) exist between the isoprene emissions predicted by each inventory, with MEGAN emissions generally higher. Based on our simulations we estimate that tropical South America (30–85°W, 14°N–25°S) contributes about 15–35% of total global isoprene emissions. We have quantified the model sensitivity to changes in isoprene emissions, chemistry, boundary layer mixing, and soil NO_x emissions using ground-based and airborne observations. We find GEOS-Chem has difficulty reproducing several observed chemical species; typically hydroxyl concentrations are underestimated, whilst mixing ratios of isoprene and its oxidation products are overestimated. The magnitude of model formaldehyde (HCHO) columns are most sensitive to the choice of chemical mechanism and isoprene emission inventory. We find GEOS-Chem exhibits a significant positive bias (10–100%) when compared with HCHO columns from the Scanning Imaging Absorption Spectrometer for Atmospheric Chartography (SCIAMACHY) and Ozone Monitoring Instrument (OMI) for the study year 2006. Simulations that use the more detailed chemical mechanism and/or lowest isoprene emissions provide the best agreement to the satellite data, since they result in lower-HCHO columns