Bubbles on Manifolds with a U(1) Isometry

We investigate the construction of five-dimensional, three-charge supergravity solutions that only have a rotational U(1) isometry. We show that such solutions can be obtained as warped compactifications with a singular ambi-polar hyper-Kahler base space and singular warp factors. We show that the complete solution is regular around the critical surface of the ambi-polar base. We illustrate this by presenting the explicit form of the most general supersymmetric solutions that can be obtained from an Atiyah-Hitchin base space and its ambi-polar generalizations. We make a parallel analysis using an ambi-polar generalization of the Eguchi-Hanson base space metric. We also show how the bubbling procedure applied to the ambi-polar Eguchi-Hanson metric can convert it to a global AdS_2xS^3 compactification.Comment: 33 pages, 5 figures, LaTeX; references adde

Nano-Immunodetection and Quantification of Mycobacteria in Metalworking Fluids

The accurate detection and enumeration of mycobacteria in metalworking fluids (MWFs) is imperative from an environmental protection and occupational health perspective. We report here on a comparison of the labeling efficiency of nano-immunomagnetic particles (NIMP) and free antibody (FAb) to detect mycobacteria in semisynthetic MWF by using both traditional visualization analysis and cluster analysis aided visualization analysis (CAAVA). The NIMP labeling method involved coating nanometer-scale magnetic particles with Protein A, and oriented conjugation of polyclonal antibodies specific to Mycobacterium spp. The FAb labeling method is modified from the traditional immunofluorescence (IF) method for more efficient detection of mycobacteria in a model MWF. The labeling efficiency of NIMP and FAb were 7.2 ± 4.6 and 16.3 ± 5.5%, and the specificity 85.0 ± 6.1 and 88.1 ± 10.5%, respectively, based on traditional visualization analysis. Based on CAAVA analysis, the labeling efficiency of NIMP and FAb increased to 12.4 ± 1.6 and 20.5 ± 3.9%, and the specificity to 97.8 ± 3.2 and 98.5 ± 2.5%, respectively. A linear relationship of FCM counts and seeded concentrations was observed over four orders of magnitude (R 2 ≤ 0.99) in pure and ternary cultures. The results strongly support the applicability of either FAb or NIMP coupled with CAAVA and flow cytometry for rapid detection and enumeration of mycobacteria in complex matrices.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/63114/1/ees.2007.24.58.pd

Exact operator bosonization of finite number of fermions in one space dimension

We derive an exact operator bosonization of a finite number of fermions in one space dimension. The fermions can be interacting or noninteracting and can have an arbitrary hamiltonian, as long as there is a countable basis of states in the Hilbert space. In the bosonized theory the finiteness of the number of fermions appears as an ultraviolet cut-off. We discuss implications of this for the bosonized theory. We also discuss applications of our bosonization to one-dimensional fermion systems dual to (sectors of) string theory such as LLM geometries and c=1 matrix model.Comment: 47 pages, 1 figure; (v2) typos correcte

Minisuperspace Quantization of "Bubbling AdS" and Free Fermion Droplets

We quantize the space of 1/2 BPS configurations of Type IIB SUGRA found by Lin, Lunin and Maldacena (hep-th/0409174), directly in supergravity. We use the Crnkovic-Witten-Zuckerman covariant quantization method to write down the expression for the symplectic structure on this entire space of solutions. We find the symplectic form explicitly around AdS_5 x S^5 and obtain a U(1) Kac-Moody algebra, in precise agreement with the quantization of a system of N free fermions in a harmonic oscillator potential, as expected from AdS/CFT. As a cross check, we also perform the quantization around AdS_5 x S^5 by another method, using the known spectrum of physical perturbations around this background and find precise agreement with our previous calculation.Comment: 22 Pages + 2 Appendices, JHEP3; v3: explanation of factor 2 mismatch added, references reordered, published versio

Human post-mortem synapse proteome integrity screening for proteomic studies of postsynaptic complexes

Background Synapses are fundamental components of brain circuits and are disrupted in over 100 neurological and psychiatric diseases. The synapse proteome is physically organized into multiprotein complexes and polygenic mutations converge on postsynaptic complexes in schizophrenia, autism and intellectual disability. Directly characterising human synapses and their multiprotein complexes from post-mortem tissue is essential to understanding disease mechanisms. However, multiprotein complexes have not been directly isolated from human synapses and the feasibility of their isolation from post-mortem tissue is unknown. Results Here we establish a screening assay and criteria to identify post-mortem brain samples containing well-preserved synapse proteomes, revealing that neocortex samples are best preserved. We also develop a rapid method for the isolation of synapse proteomes from human brain, allowing large numbers of post-mortem samples to be processed in a short time frame. We perform the first purification and proteomic mass spectrometry analysis of MAGUK Associated Signalling Complexes (MASC) from neurosurgical and post-mortem tissue and find genetic evidence for their involvement in over seventy human brain diseases. Conclusions We have demonstrated that synaptic proteome integrity can be rapidly assessed from human post-mortem brain samples prior to its analysis with sophisticated proteomic methods. We have also shown that proteomics of synapse multiprotein complexes from well preserved post-mortem tissue is possible, obtaining structures highly similar to those isolated from biopsy tissue. Finally we have shown that MASC from human synapses are involved with over seventy brain disorders. These findings should have wide application in understanding the synaptic basis of psychiatric and other mental disorders

School-based prevention for adolescent Internet addiction: prevention is the key. A systematic literature review

Adolescents’ media use represents a normative need for information, communication, recreation and functionality, yet problematic Internet use has increased. Given the arguably alarming prevalence rates worldwide and the increasingly problematic use of gaming and social media, the need for an integration of prevention efforts appears to be timely. The aim of this systematic literature review is (i) to identify school-based prevention programmes or protocols for Internet Addiction targeting adolescents within the school context and to examine the programmes’ effectiveness, and (ii) to highlight strengths, limitations, and best practices to inform the design of new initiatives, by capitalizing on these studies’ recommendations. The findings of the reviewed studies to date presented mixed outcomes and are in need of further empirical evidence. The current review identified the following needs to be addressed in future designs to: (i) define the clinical status of Internet Addiction more precisely, (ii) use more current psychometrically robust assessment tools for the measurement of effectiveness (based on the most recent empirical developments), (iii) reconsider the main outcome of Internet time reduction as it appears to be problematic, (iv) build methodologically sound evidence-based prevention programmes, (v) focus on skill enhancement and the use of protective and harm-reducing factors, and (vi) include IA as one of the risk behaviours in multi-risk behaviour interventions. These appear to be crucial factors in addressing future research designs and the formulation of new prevention initiatives. Validated findings could then inform promising strategies for IA and gaming prevention in public policy and education

New insights into the genetic etiology of Alzheimer's disease and related dementias

Characterization of the genetic landscape of Alzheimer's disease (AD) and related dementias (ADD) provides a unique opportunity for a better understanding of the associated pathophysiological processes. We performed a two-stage genome-wide association study totaling 111,326 clinically diagnosed/'proxy' AD cases and 677,663 controls. We found 75 risk loci, of which 42 were new at the time of analysis. Pathway enrichment analyses confirmed the involvement of amyloid/tau pathways and highlighted microglia implication. Gene prioritization in the new loci identified 31 genes that were suggestive of new genetically associated processes, including the tumor necrosis factor alpha pathway through the linear ubiquitin chain assembly complex. We also built a new genetic risk score associated with the risk of future AD/dementia or progression from mild cognitive impairment to AD/dementia. The improvement in prediction led to a 1.6- to 1.9-fold increase in AD risk from the lowest to the highest decile, in addition to effects of age and the APOE ε4 allele