6 research outputs found
Salivary Metabolites in Patients with Mucopolysaccharidosis
Objective: To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material and Methods:The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 7:30 and 9:00 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4ºC, and the supernatants (500µl) were stored at -80ºC until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively. Results: Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring. Conclusion: The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls
Salivary Metabolites in Patients with Mucopolysaccharidosis
Objective: To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material and Methods:The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 7:30 and 9:00 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4ºC, and the supernatants (500µl) were stored at -80ºC until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively. Results: Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring. Conclusion: The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls
Salivary Metabolite Fingerprint of Type 1 Diabetes in Young Children
Metabolomics
is an important tool for the evaluation of the human
condition, in both health or disease. This study analyzed the salivary
components of type I diabetic children (DM1) under six years of age,
to assess oral health related to diabetes control, as well as metabolite
profiling using NMR. Partial least squared discriminant analysis (PLS-DA)
was used to compare healthy (HG) and uncontrolled DM1 subjects that
demonstrated a separation between the groups with classificatory performance
of ACC = 0.80, R<sup>2</sup> = 0.92, Q<sup>2</sup> = 0.02 and for
DM1 children with glycemia >200 mg/dL of ACC = 0.74, R<sup>2</sup> = 0.91, Q<sup>2</sup> = 0.06. The metabolites that mostly contributed
to the distinction between the groups in the loading factor were acetate,
n-acetyl-sugar, lactate, and sugar. The univariate analysis showed
a decreased salivary concentration of succinic acid and increased
levels of lactate, acetate, and sucrose in uncontrolled and DM1 children
with glycemia >200 mg/dL. The present study demonstrates that the
salivary profile of DM1 differs from that of HG children. It appears
that diabetes status control has an important effect on the salivary
composition
The relationship between unspecific s-IgA and dental caries: A systematic review and meta-analysis
Mother-to-child transmission of Streptococcus mutans: A systematic review and meta-analysis
Characterisation of microbial attack on archaeological bone
As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved