Mapping the Differential Distribution of Glycosaminoglycans in the Adult Human Retina, Choroid, and Sclera

The authors provide a detailed analysis of the compartmentalization in the human retina of glycosaminoglycans (GAGs), by both chain type and sulfation pattern. This reference map provides a basis for understanding the functional regulation of GAG-binding proteins in health and disease processes

Development of a microtiter plate-based glycosaminoglycan array for the investigation of glycosaminoglycan-protein interactions

The interactions of glycosaminoglycans (GAGs) with proteins underlie a wide range of important biological processes. However, the study of such binding reactions has been hampered by the lack of a simple frontline analysis technique. Previously, we have reported that cold plasma polymerization can be used to coat microtiter plate surfaces with allyl amine to which GAGs (e.g. heparin) can be non-covalently immobilized retaining their ability to interact with proteins. Here we have assessed the capabilities of surface coats derived from different ratios of allyl amine and octadiene (100:0 to 0:100) to support the binding of diverse GAGs (e.g. chondroitin-4-sulfate, dermatan sulfate, heparin preparations and hyaluronan) in a functionally active state. The Link module from TSG-6 was used as a probe to determine the level of functional binding because of its broad (and unique) specificity for both sulfated and non-sulfated GAGs. All of the GAGs tested could bind this domain following their immobilization, although there were clear differences in their protein-binding activities depending on the surface chemistry to which they were adsorbed. On the basis of these experiments 100% allyl amine was chosen for the generation of a microtiter plate-based “sugar array”; X-ray photoelectron spectroscopy revealed that similar relative amounts of chondroitin-4-sulfate, dermatan sulfate and heparin (including two selectively de-sulfated derivatives) were immobilized onto this surface. Analysis of four unrelated proteins (i.e. TSG-6, complement factor H, fibrillin-1 and versican) illustrated the utility of this array to determine the GAG-binding profile and specificity for a particular target protein

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Not AvailableAlthough oil-sardine fishery is a major fishery on the west coast, it is a minor fishery in some places on the east coast. Information about Sardinella longiceps (valenciennes) on the east coast is scanty and the total catch landed at Parangipettai was about 79.95 tonnes from October 1985 to September 1986. Oil-sardine fishery was dominant in Parangipettai during July 1986 to September .86 amounting to about 70.5 tonnes. The length ranged from 102 to 103 mm in total length and nearly 60% of the catch comprised of fish above 160 mm In length. The size at first maturity was 156 mm for females and 158.5 mm for males and spawning was found to occur from July 1986 to September'86. A comprehensive study of the occurrence and biology of oil-sardine, S longiceps on the east coast Is needed to assess the resource potential.Not Availabl