Fermi surface, possible unconventional fermions, and unusually robust resistive critical fields in the chiral-structured superconductor AuBe

The noncentrosymmetric superconductor (NCS) AuBe is investigated using a variety of thermodynamic and resistive probes in magnetic fields of up to 65~T and temperatures down to 0.3~K. Despite the polycrystalline nature of the samples, the observation of a complex series of de Haas-van Alphen (dHvA) oscillations has allowed the calculated bandstructure for AuBe to be validated. This permits a variety of BCS parameters describing the superconductivity to be estimated, despite the complexity of the measured Fermi surface. In addition, AuBe displays a nonstandard field dependence of the phase of dHvA oscillations associated with a band thought to host unconventional fermions in this chiral lattice. This result demonstrates the power of the dHvA effect to establish the properties of a single band despite the presence of other electronic bands with a larger density of states, even in polycrystalline samples. In common with several other NCSs, we find that the resistive upper critical field exceeds that measured by heat capacity and magnetization by a considerable factor. We suggest that our data exclude mechanisms for such an effect associated with disorder, implying that topologically protected superconducting surface states may be involved

Discovery of Small-Molecule Enhancers of Reactive Oxygen Species That are Nontoxic or Cause Genotype-Selective Cell Death

Elevation of reactive oxygen species (ROS) levels has been observed in many cancer cells relative to nontransformed cells, and recent reports have suggested that small-molecule enhancers of ROS may selectively kill cancer cells in various in vitro and in vivo models. We used a high-throughput screening approach to identify several hundred small-molecule enhancers of ROS in a human osteosarcoma cell line. A minority of these compounds diminished the viability of cancer cell lines, indicating that ROS elevation by small molecules is insufficient to induce death of cancer cell lines. Three chemical probes (BRD5459, BRD56491, BRD9092) are highlighted that most strongly elevate markers of oxidative stress without causing cell death and may be of use in a variety of cellular settings. For example, combining nontoxic ROS-enhancing probes with nontoxic doses of l-buthionine sulfoximine, an inhibitor of glutathione synthesis previously studied in cancer patients, led to potent cell death in more than 20 cases, suggesting that even nontoxic ROS-enhancing treatments may warrant exploration in combination strategies. Additionally, a few ROS-enhancing compounds that contain sites of electrophilicity, including piperlongumine, show selective toxicity for transformed cells over nontransformed cells in an engineered cell-line model of tumorigenesis. These studies suggest that cancer cell lines are more resilient to chemically induced increases in ROS levels than previously thought and highlight electrophilicity as a property that may be more closely associated with cancer-selective cell death than ROS elevation.Chemistry and Chemical Biolog

A Small-Molecule Probe of the Histone Methyltransferase G9a Induces Cellular Senescence in Pancreatic Adenocarcinoma

Post-translational modifications of histones alter chromatin structure and play key roles in gene expression and specification of cell states. Small molecules that target chromatin-modifying enzymes selectively are useful as probes and have promise as therapeutics, although very few are currently available. G9a (also named euchromatin histone methyltransferase 2 (EHMT2)) catalyzes methylation of lysine 9 on histone H3 (H3K9), a modification linked to aberrant silencing of tumor-suppressor genes, among others. Here, we report the discovery of a novel histone methyltransferase inhibitor, BRD4770. This compound reduced cellular levels of di- and trimethylated H3K9 without inducing apoptosis, induced senescence, and inhibited both anchorage-dependent and -independent proliferation in the pancreatic cancer cell line PANC-1. ATM-pathway activation, caused by either genetic or small-molecule inhibition of G9a, may mediate BRD4770-induced cell senescence. BRD4770 may be a useful tool to study G9a and its role in senescence and cancer cell biology.Chemistry and Chemical Biolog

A large-scale examination of the effectiveness of anonymous marking in reducing group performance differences in higher education assessment

The present research aims to more fully explore the issues of performance differences in higher education assessment, particularly in the context of a common measure taken to address them. The rationale for the study is that, while performance differences in written examinations are relatively well researched, few studies have examined the efficacy of anonymous marking in reducing these performance differences, particularly in modern student populations. By examining a large archive (N = 30674) of assessment data spanning a twelve-year period, the relationship between assessment marks and factors such as ethnic group, gender and socio-environmental background was investigated. In particular, analysis focused on the impact that the implementation of anonymous marking for assessment of written examinations and coursework has had on the magnitude of mean score differences between demographic groups of students. While group differences were found to be pervasive in higher education assessment, these differences were observed to be relatively small in practical terms. Further, it appears that the introduction of anonymous marking has had a negligible effect in reducing them. The implications of these results are discussed, focusing on two issues, firstly a defence of examinations as a fair and legitimate form of assessment in Higher Education, and, secondly, a call for the re-examination of the efficacy of anonymous marking in reducing group performance differences

Constraints on massive star formation: Cygnus OB2 was always an association

We examine substructure and mass segregation in the massive OB association Cygnus OB2 to better understand its initial conditions. Using a well-understood Chandra X-ray selected sample of young stars, we find that Cyg OB2 exhibits considerable physical substructure and has no evidence for mass segregation, both indications that the association is not dynamically evolved. Combined with previous kinematical studies we conclude that Cyg OB2 is dynamically very young, and what we observe now is very close to its initial conditions: Cyg OB2 formed as a highly substructured, unbound association with a low volume density (<100 stars pc−3). This is inconsistent with the idea that all stars form in dense, compact clusters. The massive stars in Cyg OB2 show no evidence for having formed particularly close to one another, nor in regions of higher than average density. Since Cyg OB2 contains stars as massive as ∼100 M⊙, this result suggests that very massive stars can be born in relatively low-density environments. This would imply that massive stars in Cyg OB2 did not form by competitive accretion, or by mergers

"They go for gender first"

There have been many recent media reports about the online harassment of women journalists working in technology, particularly the video gaming industry. However, little research has focused on this aspect, by looking at specific occupations, or analysing the implications for women and society. This paper is a feminist study of the experiences of sexist abuse of a sample of women journalists writing about technology. It is a commentary on the results of a questionnaire-based study of 102 women (and their approximately 300 comments) that work in what has emerged as one of the frontlines of the struggle for gender equality. The research looks at the extent of the abuse, the harm it causes and how women are reacting to it. Most of the participants have experienced abuse, many have changed their working practices and some have disguised their identity to avoid it. An examination of their comments suggests that sexist abuse is now often normalised, alongside a new kind of "invisible" feminism. It also reveals a mood of defiance and an appetite for radical change to address the problems of exclusion and loss of identity. Overall, results indicate that the abuse is damaging women’s lives and impacting journalism and society in a negative way

A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation

Many important cellular processes are regulated by reaction-diffusion (RD) of molecules that takes place both in the cytoplasm and on the membrane. To model and analyze such multicompartmental processes, we developed a lattice-based Monte Carlo method, Spatiocyte that supports RD in volume and surface compartments at single molecule resolution. Stochasticity in RD and the excluded volume effect brought by intracellular molecular crowding, both of which can significantly affect RD and thus, cellular processes, are also supported. We verified the method by comparing simulation results of diffusion, irreversible and reversible reactions with the predicted analytical and best available numerical solutions. Moreover, to directly compare the localization patterns of molecules in fluorescence microscopy images with simulation, we devised a visualization method that mimics the microphotography process by showing the trajectory of simulated molecules averaged according to the camera exposure time. In the rod-shaped bacterium _Escherichia coli_, the division site is suppressed at the cell poles by periodic pole-to-pole oscillations of the Min proteins (MinC, MinD and MinE) arising from carefully orchestrated RD in both cytoplasm and membrane compartments. Using Spatiocyte we could model and reproduce the _in vivo_ MinDE localization dynamics by accounting for the established properties of MinE. Our results suggest that the MinE ring, which is essential in preventing polar septation, is largely composed of MinE that is transiently attached to the membrane independently after recruited by MinD. Overall, Spatiocyte allows simulation and visualization of complex spatial and reaction-diffusion mediated cellular processes in volumes and surfaces. As we showed, it can potentially provide mechanistic insights otherwise difficult to obtain experimentally

Spatio-temporal Models of Lymphangiogenesis in Wound Healing

Several studies suggest that one possible cause of impaired wound healing is failed or insufficient lymphangiogenesis, that is the formation of new lymphatic capillaries. Although many mathematical models have been developed to describe the formation of blood capillaries (angiogenesis), very few have been proposed for the regeneration of the lymphatic network. Lymphangiogenesis is a markedly different process from angiogenesis, occurring at different times and in response to different chemical stimuli. Two main hypotheses have been proposed: 1) lymphatic capillaries sprout from existing interrupted ones at the edge of the wound in analogy to the blood angiogenesis case; 2) lymphatic endothelial cells first pool in the wound region following the lymph flow and then, once sufficiently populated, start to form a network. Here we present two PDE models describing lymphangiogenesis according to these two different hypotheses. Further, we include the effect of advection due to interstitial flow and lymph flow coming from open capillaries. The variables represent different cell densities and growth factor concentrations, and where possible the parameters are estimated from biological data. The models are then solved numerically and the results are compared with the available biological literature.Comment: 29 pages, 9 Figures, 6 Tables (39 figure files in total

ISO-LWS two colour diagram of young stellar objects

We present a [60--100] vs. [100--170] $\mu$m two colour diagram for a sample of 61 young stellar objects (YSOs) observed with the Long Wavelength Spectrometer (LWS) on-board the Infrared Space Observatory (ISO). The sample consists of 17 Class 0 sources, 15 Class I, 9 Bright Class I ($L_{\rm bol}>10^4 L_\odot$), 20 Class II (14 Herbig Ae/Be stars and 6 T Tauri stars). We find that each class occupies a well defined region in our diagram with colour temperatures increasing from Class 0 to Class II. Therefore the [60--100] vs. [100--170] two colours diagram is a powerful and simple tool to derive from future (e.g. with the Herschel Space Observatory) photometric surveys the evolutionary status of YSOs. The advantage over other tools already developed is that photometry at other wavelengths is not required: three flux measurements are enough to derive the evolutionary status of a source. As an example we use the colours of the YSO IRAS 18148$-$0440 to classify it as Class I. The main limitation of this work is the low spatial resolution of the LWS which, for some objects, causes a high uncertainty in the measured fluxes due to the background emission or to the source confusion inside the LWS beam.Comment: 7 pages, 2 figures, requires mn2e document class. Accepted by MNRA

VLTI/AMBER spectro-interferometry of the Herbig Be star MWC 297 with spectral resolution 12 000

Circumstellar disks and outflows play a fundamental role in star formation. Infrared spectro-interferometry allows the inner accretion-ejection region to be resolved. We measured interferometric visibilities, wavelength-differential phases, and closure phases of MWC 297 with a spectral resolution of 12000. To interpret our MWC 297 observations, we employed disk-wind models. The measured continuum visibilities confirm previous results that the continuum-emitting region of MWC 297 is remarkably compact. We derive a continuum ring-fit radius of ~2.2 mas (~0.56 AU at a distance of 250 pc), which is ~5.4 times smaller than the 3 AU dust sublimation radius expected for silicate grains (in the absence of radiation-shielding material). The strongly wavelength-dependent and asymmetric Br gamma-emitting region is more extended (~2.7 times) than the continuum-emitting region. At the center of the Br gamma line, we derive a Gaussian fit radius of ~6.3 mas HWHM (~1.6 AU). To interpret the observations, we employ a magneto-centrifugally driven disk-wind model consisting of an accretion disk, which emits the observed continuum radiation, and a disk wind, which emits the Br gamma line. The calculated wavelength-dependent model intensity distributions and Br gamma line profiles are compared with the observations (i.e., K-band spectrum, visibilities, differential phases, and closure phases). The closest fitting model predicts a continuum-emitting disk with an inner radius of ~0.3 AU and a disk wind ejection region with an inner radius of ~0.5 AU (~17.5 stellar radii). We obtain a disk-wind half-opening angle (the angle between the rotation axis and the innermost streamline of the disk wind) of ~80 degrees, which is larger than in T Tau models, and a disk inclination angle of ~20 degrees (i.e., almost pole-on).Comment: Astron. & Astrophys. accepte