Biosignal Telemetry

Import 22/07/2015Práce se zabývá problematikou bezdrátového přenosu změřeného EKG signálu do počítače. K přenosu dat je použita bezdrátová komunikace standardu IEEE 802.15.4. To znamená, že pracuje ve frekvenčním pásmu 2,4 GHz, přesněji v rozsahu 2405 – 2480 MHz. Bezdrátová komunikace byla realizována moduly JN5148 001 M00 od firmy NXP, a to kvůli jejich nízké spotřebě (ve vysílacím módu 17,5 mA). Moduly jsou naprogramovány tak, aby fungovaly pouze jako bezdrátová sériová linka. Ke snímání EKG signálu byl použit modul EMI12 vyráběný firmou NXP. EMI12 má spotřebu do 90 mA. Může snímat až 12 svodů s různou vzorkovací frekvencí (100, 200, 500 a 1000 Hz). Dále lze hardwarově nastavit přenosovou rychlost na 38 400, 115 200, 230 400 nebo 921 600 bit/sec. Navržený software dává uživateli na výběr ze zobrazování tří, šesti, nebo dvanácti svodů. Veškeré naměřené hodnoty mohou být uloženy ve formátu .csv.This work deals with the issue of measured wireless ECG signal transfer to computer. To data transmission is used wireless communication based on IEEE 802.15.4 standard. It means it works on 2,4 GHz frequency band, precisely in range from 2405 to 2480 MHz. Wireless communication were implemented by modules JN5148 001 M00 from NXP company, because of low consumption (in a transmitting mode 17,5 mA). Modules are programmed to work only as wireless serial port. To read ECG signal, module EMI12 was used. It was developed by Corscince company. EMI12’s consumption is maximally 90 mA. It may read up to 12 leads with different sampling rate (100, 200, 500 and 1000 Hz). Furthermore, the baud rate could be mechanically set on 38 400, 115 200, 230 400 or 921 600 bit / sec. Created software provides user a choice of showing three, six, or twelve leads. All measured values can be saved as .csv file.450 - Katedra kybernetiky a biomedicínského inženýrstvívýborn

Measurement and Visualization of the Pathological Changes in the Dermis

Import 23/08/2017Práce se zabývá měřením tkáňových abnormalit v podkoží. K měření se využívají silové kapacitní senzory od firmy SingleTact. Ty snímají odchylky v tuhosti tkáně, které mohou být způsobeny podkožními lézemi, jako jsou například podkožní nádory. Jedná se o lineárně uspořádanou sadu senzorů snímajících sílu, jež je vyvolána působením sondy na vyšetřovanou oblast. Odchylky hodnot pod jednotlivými senzory jsou zaznamenávány do grafu, ze kterého lze jednoduše vyčíst, pod jakým senzorem změna nastala a její velikost. Tento způsob měření by měl sloužit jako levné orientační měření sloužící k zjišťování podkožních nádorů.This work deals with measurement of subcutaneous abnormalities. For the measurement, the capacitive tactile sensors from company SingleTact are used. Those are detecting differences in tissue stiffness, which could be sign of presence of some subcutaneous lesions, e.g. subcutaneous tumors. To be specific, there are three linearly oriented sensors detecting reaction force, which is invoked by probe touching investigated area. Force differences under every sensor are recorded to the graphs, from which the location and size of the force difference could be easily read. This type of measurement should provide cheap, tentative mean of subcutaneous tumor detection.450 - Katedra kybernetiky a biomedicínského inženýrstvívýborn

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

<p>Abstract</p> <p>Background</p> <p>The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates.</p> <p>Methods</p> <p>A multi-species indirect immunosorbent assay (iELISA) using <it>Brucella </it>S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (<it>Sus scrofa</it>), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs.</p> <p>Results</p> <p>Mean apparent prevalence below 0.5% was identified in chamois (<it>Rupicapra pyrenaica</it>), Iberian wild goat (<it>Capra pyrenaica</it>), and red deer (<it>Cervus elaphus</it>). Roe deer (<it>Capreolus capreolus</it>), fallow deer (<it>Dama dama</it>), mouflon (<it>Ovis aries</it>) and Barbary sheep (<it>Ammotragus lervia</it>) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating <it>B. abortus </it>biovar 1 and <it>B. melitensis </it>biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as <it>B. suis </it>biovar 2. DNA polymorphisms were similar to those found in domestic pigs.</p> <p>Conclusions</p> <p>In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.</p

Fitcentrum - malý a střední podnik

Import 20/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Ekonomická fakulta. Katedra (713) tělesné výchovy a sport

Projekt kulturní akce

Import 20/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Ekonomická fakulta. Katedra (115) management

FURTHER EVIDENCE ON THE EFFECTIVENESS OF THE CALIFORNIA LAND CONSERVATION ACT

The effectiveness of use-value assessment provided under the California Land Conservation Act in maintaining open space and in deterring the conversion of prime agricultural land to urban uses is evaluated in the San Joaquin subbasin for the period 1958-1974. Results from the prime land resource use-stock model indicate that the Act is a significant policy variable exhibiting the intended effects upon idle and agricultural land stocks, but that it is ineffective in controlling the growth of urban stocks on prime land

Mechanical thrombectomy in acute stroke : five years of experience in Poland

Objectives: Mechanical thrombectomy (MT) is not reimbursed by the Polish public health system. We present a description of 5 years of experience with MT in acute stroke in Comprehensive Stroke Centers (CSCs) in Poland. Methods and results: We retrospectively analyzed the results of a structured questionnaire from 23 out of 25 identified CSCs and 22 data sets that include 61 clinical, radiological and outcome measures. Results: Most of the CSCs (74%) were founded at University Hospitals and most (65.2%) work round the clock. In 78.3% of them, the working teams are composed of neurologists and neuro-radiologists. All CSCs perform CT and angio-CT before MT. In total 586 patients were subjected to MT and data from 531 of them were analyzed. Mean time laps from stroke onset to groin puncture was 250 99 min. 90.3% of the studied patients had MT within 6 h from stroke onset; 59.3% of them were treated with IV rt-PA prior to MT; 15.1% had IA rt-PA during MT and 4.7% - emergent stenting of a large vessel. M1 of MCA was occluded in 47.8% of cases. The Solitaire device was used in 53% of cases. Successful recanalization (TICI2b–TICI3) was achieved in 64.6% of cases and 53.4% of patients did not experience hemorrhagic transformation. Clinical improvement on discharge was noticed in 53.7% of cases, futile recanalization - in 30.7%, mRS of 0–2 - in 31.4% and mRS of 6 in 22% of cases. Conclusion: Our results can help harmonize standards for MT in Poland according to international guideline

A novel epileptic encephalopathy mutation in KCNB1 disrupts Kv2.1 ion selectivity, expression, and localization

The epileptic encephalopathies are a group of highly heterogeneous genetic disorders. The majority of disease-causing mutations alter genes encoding voltage-gated ion channels, neurotransmitter receptors, or synaptic proteins. We have identified a novel de novo pathogenic K(+) channel variant in an idiopathic epileptic encephalopathy family. Here, we report the effects of this mutation on channel function and heterologous expression in cell lines. We present a case report of infantile epileptic encephalopathy in a young girl, and trio-exome sequencing to determine the genetic etiology of her disorder. The patient was heterozygous for a de novo missense variant in the coding region of the KCNB1 gene, c.1133T>C. The variant encodes a V378A mutation in the α subunit of the Kv2.1 voltage-gated K(+) channel, which is expressed at high levels in central neurons and is an important regulator of neuronal excitability. We found that expression of the V378A variant results in voltage-activated currents that are sensitive to the selective Kv2 channel blocker guangxitoxin-1E. These voltage-activated Kv2.1 V378A currents were nonselective among monovalent cations. Striking cell background–dependent differences in expression and subcellular localization of the V378A mutation were observed in heterologous cells. Further, coexpression of V378A subunits and wild-type Kv2.1 subunits reciprocally affects their respective trafficking characteristics. A recent study reported epileptic encephalopathy-linked missense variants that render Kv2.1 a tonically activated, nonselective cation channel that is not voltage activated. Our findings strengthen the correlation between mutations that result in loss of Kv2.1 ion selectivity and development of epileptic encephalopathy. However, the strong voltage sensitivity of currents from the V378A mutant indicates that the loss of voltage-sensitive gating seen in all other reported disease mutants is not required for an epileptic encephalopathy phenotype. In addition to electrophysiological differences, we suggest that defects in expression and subcellular localization of Kv2.1 V378A channels could contribute to the pathophysiology of this KCNB1 variant