UrbanDig Project: sport practices and artistic interventions for co-creating urban space

The 'UrbanDig Project' has been created by the non-for-profit organisation Ohi Paizoume, based in Athens, Greece. Behind the project lies the motivation to tackle the need for civic engagement and interaction with the urban space (either open/public spaces or private buildings), while suggesting that this cannot be a sterilized process but an ongoing, continuously unfinished “reporting from the front” of the (un-)measurable, the (in-)visible, the (un-)real. The organisation puts at the heart of its work the creation of site-specific content that forms the foundation of community programs researching the cultural capital of a neighbourhood, bringing people from various ages, backgrounds and disciplines (including arts and sport) together. Although primarily an arts-based practice, sport is among the key methodological tools used in the research and civic engagement process, while there is a strong sports-related component in the site specific performances that take place at the end of each project as a means of celebration of the collective effort and the creation of new/ enhanced narratives for each neighbourhood’s storytelling. In this paper we aim to elaborate how sport and arts can sharply intervene and contribute to co-producing urban space and wellbeing. To illustrate these points, we are using the example of Urban Dig Project, Xouthou Street, an intervention in the urban space of Greece

A large sample analysis of European rivers on seasonal river flow correlation and its physical drivers

The geophysical and hydrological processes governing river flow formation exhibit persistence at several timescales, which may manifest itself with the presence of positive seasonal correlation of streamflow at several different time lags. We investigate here how persistence propagates along subsequent seasons and affects low and high flows. We define the high-flow season (HFS) and the low-flow season (LFS) as the 3-month and the 1-month periods which usually exhibit the higher and lower river flows, respectively. A dataset of 224 rivers from six European countries spanning more than 50 years of daily flow data is exploited. We compute the lagged seasonal correlation between selected river flow signatures, in HFS and LFS, and the average river flow in the antecedent months. Signatures are peak and average river flow for HFS and LFS, respectively. We investigate the links between seasonal streamflow correlation and various physiographic catchment characteristics and hydro-climatic properties. We find persistence to be more intense for LFS signatures than HFS. To exploit the seasonal correlation in the frequency estimation of high and low flows, we fit a bi-variate meta-Gaussian probability distribution to the selected flow signatures and average flow in the antecedent months in order to condition the distribution of high and low flows in the HFS and LFS, respectively, upon river flow observations in the previous months. The benefit of the suggested methodology is demonstrated by updating the frequency distribution of high and low flows one season in advance in a real-world case. Our findings suggest that there is a traceable physical basis for river memory which, in turn, can be statistically assimilated into high- and low-flow frequency estimation to reduce uncertainty and improve predictions for technical purposes

Motives and barriers to development of organic olive production

Environmental and health problems caused by conventional agriculture lead many farmers to organic agriculture, a system that has been rapidly developed worldwide. However, the adoption of organic farming is not an easy decision and it carries with it several barriers. Different interrelated factors are usually at play, rather than one decisive single motive, concerning financial, environmental, health issues and personal beliefs and values. This study aims to indentify the factors that encourage olive growers to adopt organic practices and the reasons that discourage them to continue. Moreover, it aims to answer the question whether the farmers would continue to grow in organic way, even if the compensatory payments will be reduced? The research is conducted in Lesvos Island, in Greece and a structured questionnaire was distributed to 69 olive growers. Results illustrate that financial factors are especially important incentives for conversion. On the other hand, institutional aspects are the most important problems that organic farmers face during production process. Finally, 53.6% of interviewed farmers express their intention of reversion from organic agriculture, due to variable problems. Results could help to better understand motives and barriers that lie behind farmers’ choices, in order to support the potential for the development of organic olive production

A stochastic model for the hourly solar radiation process for application in renewable resources management

Since the beginning of the 21st century, the scientific community has made huge leaps to exploit renewable energy sources, with solar radiation being one of the most important. However, the variability of solar radiation has a significant impact on solar energy conversion systems, such as in photovoltaic systems, characterized by a fast and non-linear response to incident solar radiation. The performance prediction of these systems is typically based on hourly or daily data because those are usually available at these time scales. The aim of this work is to investigate the stochastic nature and time evolution of the solar radiation process for daily and hourly scale, with the ultimate goal of creating a new cyclostationary stochastic model capable of reproducing the dependence structure and the marginal distribution of hourly solar radiation via the clearness index KT.</p

Rapid generation of human B-cell lymphomas via combined expression of Myc and Bcl2 and their use as a preclinical model for biological therapies

Although numerous mouse models of B-cell malignancy have been developed via the enforced expression of defined oncogenic lesions, the feasibility of generating lineage-defined human B-cell malignancies using mice reconstituted with modified human hematopoietic stem cells (HSCs) remains unclear. In fact, whether human cells can be transformed as readily as murine cells by simple oncogene combinations is a subject of considerable debate. Here, we describe the development of humanized mouse model of MYC/BCL2-driven ‘double-hit’ lymphoma. By engrafting human HSCs transduced with the oncogene combination into immunodeficient mice, we generate a fatal B malignancy with complete penetrance. This humanized-MYC/BCL2-model (hMB) accurately recapitulates the histopathological and clinical aspects of steroid-, chemotherapy- and rituximab-resistant human ‘double-hit’ lymphomas that involve the MYC and BCL2 loci. Notably, this model can serve as a platform for the evaluation of antibody-based therapeutics. As a proof of principle, we used this model to show that the anti-CD52 antibody alemtuzumab effectively eliminates lymphoma cells from the spleen, liver and peripheral blood, but not from the brain. The hMB humanized mouse model underscores the synergy of MYC and BCL2 in ‘double-hit’ lymphomas in human patients. Additionally, our findings highlight the utility of humanized mouse models in interrogating therapeutic approaches, particularly human-specific monoclonal antibodies.Kathy and Curt Marble Cancer Research FundSingapore-MIT Alliance for Research and TechnologyNational Institutes of Health (U.S.) (Grant R01-CA128803)Virginia and Daniel K. Ludwig Graduate FellowshipNational Institute of General Medical Sciences (U.S.) (Medical Scientist Training Program Grant T32GM007753)MIT School of Science (Cancer Research Fellowship

Quality of life assessment in companion animals: what, why, who, when and how

Quality of life is a commonly used phrase in veterinary medicine. It describes a complex evaluation that may be difficult for animals to perform, and the phrase “happiness” may be a more crude but useful approximation. Quality of life assessments should ideally be an integral part of our decision making, and should encompass evaluation of aspects of a pet’s life beyond just its health. Assessments should aim both to evaluate an animal’s quality of life, and to look for ways in which it might be improved. This article will discuss the challenges of assessing quality of life in companion animals, and will review the range of different methods available for assessment of quality of life in cats and dogs

Membrane-Bound IL-21 Promotes Sustained Ex Vivo Proliferation of Human Natural Killer Cells

NK cells have therapeutic potential for a wide variety of human malignancies. However, because NK cells expand poorly in vitro, have limited life spans in vivo, and represent a small fraction of peripheral white blood cells, obtaining sufficient cell numbers is the major obstacle for NK-cell immunotherapy. Genetically-engineered artificial antigen-presenting cells (aAPCs) expressing membrane-bound IL-15 (mbIL15) have been used to propagate clinical-grade NK cells for human trials of adoptive immunotherapy, but ex vivo proliferation has been limited by telomere shortening. We developed K562-based aAPCs with membrane-bound IL-21 (mbIL21) and assessed their ability to support human NK-cell proliferation. In contrast to mbIL15, mbIL21-expressing aAPCs promoted log-phase NK cell expansion without evidence of senescence for up to 6 weeks of culture. By day 21, parallel expansion of NK cells from 22 donors demonstrated a mean 47,967-fold expansion (median 31,747) when co-cultured with aAPCs expressing mbIL21 compared to 825-fold expansion (median 325) with mbIL15. Despite the significant increase in proliferation, mbIL21-expanded NK cells also showed a significant increase in telomere length compared to freshly obtained NK cells, suggesting a possible mechanism for their sustained proliferation. NK cells expanded with mbIL21 were similar in phenotype and cytotoxicity to those expanded with mbIL15, with retained donor KIR repertoires and high expression of NCRs, CD16, and NKG2D, but had superior cytokine secretion. The mbIL21-expanded NK cells showed increased transcription of the activating receptor CD160, but otherwise had remarkably similar mRNA expression profiles of the 96 genes assessed. mbIL21-expanded NK cells had significant cytotoxicity against all tumor cell lines tested, retained responsiveness to inhibitory KIR ligands, and demonstrated enhanced killing via antibody-dependent cell cytotoxicity. Thus, aAPCs expressing mbIL21 promote improved proliferation of human NK cells with longer telomeres and less senescence, supporting their clinical use in propagating NK cells for adoptive immunotherapy

The role of catalyst acidity and shape selectivity on products from the catalytic fast pyrolysis of beech wood

The catalytic fast pyrolysis (CFP) of biomass represents an efficient integrated process to produce deoxygenated stable liquid fuels and valuable chemical products from lignocellulosic biomass. The zeolite ZSM-5 is a widely studied catalyst for the CFP process. However, its microporous structure may limit the diffusion of high molecular weight pyrolysis intermediates to its active sites. Mesoporous aluminosilicates such as Al-SBA-15 are promising materials with larger pore sizes that can overcome these diffusional limitations. Previous comparisons between mesoporous aluminosilicates and ZSM-5 for the CFP process have neglected the disproportionately high acidity of ZSM-5. In this study, an Al-SBA-15 catalyst has been synthesised with high acidity, comparable to that of a ZSM-5 catalyst with a Si:Al ratio of 15:1. The synthesised Al-SBA-15 catalyst was characterised by N2 physisorption, XRD and propylamine-TPD, and was compared to a ZSM-5 catalyst and a typical industrial equilibrium fluid catalytic cracking catalyst (e-FCC). All three catalysts were used at three different catalyst to biomass (C/B) ratios, to investigate the effect of varying concentrations of acid sites on the product distribution from the catalytic fast pyrolysis of beech wood. Interestingly, despite their dissimilar structural architectures, all three solid acid catalysts displayed similar reaction pathways towards the cracking of high molecular weight products such as levoglucosan and formation of intermediates including phenolics and furans. However, the selectivity towards the final catalytic products was dictated mainly by the structure of the catalysts. Despite their very similar surface area and acidity, the ZSM-5 exhibited high selectivity for the formation of desirable aromatic hydrocarbon products due to its shape-selective micropore structure, while Al-SBA-15 instead shifted the selectivity towards the formation of undesirable coke. The results highlighted the importance of catalyst shape-selectivity in the catalytic fast pyrolysis of biomass for the conversion of pyrolysis vapours into desirable products and the suppression of undesirable solid byproduct formation

Coupled stalagmite – Alluvial fan response to the 8.2 ka event and early Holocene palaeoclimate change in Greece

We explore the expression of early Holocene climatic change in the terrestrial Mediterranean of southern Greece. A regional palaeoclimate record from stable isotope and trace element geochemical proxies in an early Holocene (~12.4 ka to 6.7 ka) stalagmite is compared to the timing of palaeosol (entisol) development on an early Holocene alluvial fan located <100 km from the stalagmite site. Radiocarbon dated entisol development records fan abandonment surfaces, which can be coupled to the stalagmite climate signal. Variations in δ13C best record the main elements of palaeoclimatic change, more negative values indicating soil carbon input to karst groundwater under wetter conditions. The wettest conditions begin around 10.3 ka, coincident with the start of sapropel 1 deposition in the eastern Mediterranean. The widely documented northern hemisphere ‘8.2 ka event’ of cooler and drier conditions has a muted δ18O climatic signal in common with other stalagmite climate records from the wider Mediterranean. However, less negative δ13C values do record a period of episodic dryness between ~8.8 and ending at 8.2 ka. Wetter conditions re-established after 8.1 ka to the end of the record. The oldest alluvial fan entisols were developing by ~9.5 ka, and a prominent rubified entisol developed ~8.3 to 8.4 ka, indicating pedogenesis within dating error of the 8.2 ka event. The speleothem record of episodic dryness between ~8.8 and 8.2 ka, combined with other regional proxies, is consistent with the notion that precipitation patterns in Greece may have changed from predominantly winter frontal to summer convective during this period. Palaeosol formation on the alluvial fan may have been an allocyclic response to this change. It is plausible that fan-channel incision, driven by temporary development of a ‘flashier’ summer rainfall regime, isolated large areas of the fan surface allowing onset of prolonged pedogenesis there