Efficiency of p16 and Ki-67 immunostaining for detecting premalignant cervical lesions in high risk population

Background: This study aimed to evaluate the efficiency of immunostaining with p16 and Ki-67 in cervical cytology specimens for the detection of cervical intraepithelial neoplasia (CIN) in a high risk population.Methods: This was a prospective review of 287 women who underwent pap smear, human papilloma virus (HPV) testing and colposcopy examination, respectively. There were cervical smear abnormalities in 108 women (37.6%) and 141 patients (49.1%) tested positive for HPV. Cervical biopsy revealed normal cervix in 28 patients (9.75%), cervicitis in 48 patients (16.72%), CIN1 in 178 patients (62.02%), CIN2 in 26 patients (9.05%) and CIN3 in 7 patients (2.43%).Results: Positive staining for p16 had a sensitivity of 78.2% and a specificity of 97.4% while positive staining for Ki67 had a sensitivity of 80.6% and a specificity of 57.9% for distinguishing CIN lesions in cervical cytology specimens (p=0.001 for both). Concurrent positive staining for p16 and Ki67 in cervical cytology specimens had a sensitivity of 80.6% and a specificity of 97.4% for CIN lesions (p=0.001). Positive staining for p16 had a sensitivity of 94% and a specificity of 90.6% whereas positive staining for Ki67 had a sensitivity of 97% and a specificity of 33% for differentiating CIN lesions in colposcopic biopsy specimens (p=0.001 for both). Concurrent positive staining for p16 and Ki67 in colposcopic biopsy specimens had a sensitivity of 91% and a specificity of 94% for CIN lesions (p=0.001).Conclusions: p16/Ki-67 immunostaining applied on cervical cytology specimens can screen CIN lesions with high sensitivity and specificity in a low risk population

Synthesis and evaluation of lipase inhibitory activities of substituted 1,2,4-triazole derivatives

Pancreatic lipase (PL) plays a major role in the hydrolysis of dietary triglycerides to monoglycerides and free fatty acids in the small intestine before absorption of fats. The excessive consumption of dietary fat (triglyceride) and not to utilize it for energy production can cause an increase in obesity. Obesity is one of the serious health problem in the world and leads to many diseases such as some types of cancer, heart disease, gallstones, sleep apnea, fatty liver disease, type-2 diabetes, hypertension, coronary artery disease. Therefore, lipase is the target enzyme to prevent these diseases and the inhibitors of lipase are very important molecules as drug candidate molecules. In this study, fifteen new heterocyclic compounds have been synthesized starting from 2-[3-(4-chlorobenzyl)-5-(4-chlorophenyl)-1H-1,2,4-triazol-4-yl]-acetohydrazide and their anti-lipase activities have been examined. According to in vitro inhibition studies, molecule 2e is found to be the most potent inhibitor with the lowest IC50 value. Docking studies' results have substantially supported this result and it is seen that compound 2e is one of the four molecules with the highest binding affinity. This molecule binds to the enzyme in its binding pocket by means of weak interactions with mainly Ile79, Asp80, Val260, Arg257 and His264.

Synthesis and evaluation of lipase inhibitory activities of substituted 1,2,4-triazole derivatives

1409-1417Pancreatic lipase (PL) plays a major role in the hydrolysis of dietary triglycerides to monoglycerides and free fatty acids in the small intestine before absorption of fats. The excessive consumption of dietary fat (triglyceride) and not to utilize it for energy production can cause an increase in obesity. Obesity is one of the serious health problem in the world and leads to many diseases such as some types of cancer, heart disease, gallstones, sleep apnea, fatty liver disease, type-2 diabetes, hypertension, coronary artery disease. Therefore, lipase is the target enzyme to prevent these diseases and the inhibitors of lipase are very important molecules as drug candidate molecules. In this study, fifteen new heterocyclic compounds have been synthesized starting from 2-[3-(4-chlorobenzyl)-5-(4-chlorophenyl)-1H-1,2,4-triazol-4-yl]-acetohydrazide and their anti-lipase activities have been examined. According to in vitro inhibition studies, molecule 2e is found to be the most potent inhibitor with the lowest IC50 value. Docking studies' results have substantially supported this result and it is seen that compound 2e is one of the four molecules with the highest binding affinity. This molecule binds to the enzyme in its binding pocket by means of weak interactions with mainly Ile79, Asp80, Val260, Arg257 and His264

Mentoring Relations in the Aircraft Industry: A Case Study In Turkey

The aim of this study is to evaluate the mentoring process in a private organisation which utilises a very high technology and innovation process. In this organisation, the top managers consider themselves as informal mentors who facilitate the transfer of the organisational culture and knowledge to the working personnel, especially to the engineers. From this perspective, the study investigated the relationship of perceived mentoring functions and gender of the mentees and gender of the dyad relationships in a sample of 85 white-collar employees. Furthermore, interpersonal trust was analysed as the determinant of perceived mentoring relationships. As hypothesised, interpersonal trust was found as a strong factor in mentoring relationships. However, gender of the mentees and gender of the dyad mentoring failed to make significant contributions to perceived mentoring relationships. The findings from this preliminary study suggest that the need for more in-depth research on multicultural issues in mentoring. Specifically organisations under the impact of modern technology and management need more future multicultural quantitative studies

A Serous Cystadenocarcinoma of the Pancreas Diagnosed With Lymph Node Metastasis

Serous cystic neoplasms of the pancreas account for 10% to 16% of all pancreatic cystic masses. Serous cystic neoplasms were evaluated as benign pancreatic masses. For all that, the first serous cystic neoplasm with malignancy criteria was described by George in 1989. Only 10 cases have been observed until today. A 53-year-old female patient presented with complaints of jaundice and abdominal pain. Her past medical history revealed pancreatic cysts during the examination for abdominal pain. Computed tomography revealed a cystic mass of approximately 8 cm in size with a solid component originating from the head of the pancreas and leading to obstruction in the bile duct. She underwent pylorus-preserving pancreaticoduodenectomy and was discharged on Day 12 due to the absence of any surgical abnormality during the postoperative follow-up. On examination of the surgical specimens, a multiloculated cystic tumor with a serous content was detected. Tumor metastasis which demonstrated a positive reaction with cytokeratin 7 and cytokeratin 19 in the celiac lymph node biopsies was detected. The tumor was found to have a histomorphologically benign appearance and was reported as a serous cystadenocarcinoma based on the desmoplastic stroma and lymph node metastasis. Cystic neoplasms of the pancreas can be followed conservatively. Malignant transformation in pancreatic serous cystadenocarcinoma should be kept in minds such as pancreatitis, bile duct obstruction, and new-onset or increased complaints during follow-up

Azimuthal anisotropy of charged jet production in root s(NN)=2.76 TeV Pb-Pb collisions

We present measurements of the azimuthal dependence of charged jet production in central and semi-central root s(NN) = 2.76 TeV Pb-Pb collisions with respect to the second harmonic event plane, quantified as nu(ch)(2) (jet). Jet finding is performed employing the anti-k(T) algorithm with a resolution parameter R = 0.2 using charged tracks from the ALICE tracking system. The contribution of the azimuthal anisotropy of the underlying event is taken into account event-by-event. The remaining (statistical) region-to-region fluctuations are removed on an ensemble basis by unfolding the jet spectra for different event plane orientations independently. Significant non-zero nu(ch)(2) (jet) is observed in semi-central collisions (30-50% centrality) for 20 <p(T)(ch) (jet) <90 GeV/c. The azimuthal dependence of the charged jet production is similar to the dependence observed for jets comprising both charged and neutral fragments, and compatible with measurements of the nu(2) of single charged particles at high p(T). Good agreement between the data and predictions from JEWEL, an event generator simulating parton shower evolution in the presence of a dense QCD medium, is found in semi-central collisions. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Peer reviewe

Pseudorapidity and transverse-momentum distributions of charged particles in proton-proton collisions at root s=13 TeV

The pseudorapidity (eta) and transverse-momentum (p(T)) distributions of charged particles produced in proton-proton collisions are measured at the centre-of-mass energy root s = 13 TeV. The pseudorapidity distribution in vertical bar eta vertical bar <1.8 is reported for inelastic events and for events with at least one charged particle in vertical bar eta vertical bar <1. The pseudorapidity density of charged particles produced in the pseudorapidity region vertical bar eta vertical bar <0.5 is 5.31 +/- 0.18 and 6.46 +/- 0.19 for the two event classes, respectively. The transverse-momentum distribution of charged particles is measured in the range 0.15 <p(T) <20 GeV/c and vertical bar eta vertical bar <0.8 for events with at least one charged particle in vertical bar eta vertical bar <1. The evolution of the transverse momentum spectra of charged particles is also investigated as a function of event multiplicity. The results are compared with calculations from PYTHIA and EPOS Monte Carlo generators. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Peer reviewe

Effect of Saliva Contamination on Microleakage of Open Sandwich Restorations

celik, Cigdem/0000-0002-5936-0196WOS:000575915100005PubMed: 33132390Objectives: The purpose of the present study was to evaluate the microleakage of conventional glass-ionomer, resin modified glass-ionomer and glass hybrid ionomer Class II open sandwich restorations with or without saliva contamination. Material and methods: Sixty extracted sound human molar teeth were used and 120 class II slot cavities were prepared in mesial and distal surfaces. The gingival margins were located 1 mm below the cementoenamel junction. All specimens were randomly divided in 4 groups (n=15): Group I: High-Viscous Glass Ionomer (Fuji IX GP) Group II: Resin Modified Glass Ionomer (Fuji II LC) Group III: Glass Hybrid Ionomer (Equia-fil Forte), Group IV: Composite Resin (G'aenial Posterior). In open sandwich restoration groups, glass ionomer materials were placed to gingival floor in 1 mm thickness and rest of the cavity was filled with resin composite. After the restorations in mesial surfaces had been performed, distal cavities were restored with the same protocol after saliva contamination. The specimens were thermo-cycled for 10000 cycles at 5 degrees C to 55 degrees C and immersed in methylene blue dye solution (% 0,5) for 24 hours. Then, they were sectioned vertically through the center of the restorations from mesial to distal surface with a water-cooled diamond saw with 1mm thickness. Subsequently, the dye penetration was evaluated with image analysis software. Data were statistically analyzed (p0.05). Additionally, there was not a statistically significant difference between the no contamination and saliva contaminated groups regardless of dental materials tested (p>0.05). Conclusion: Within the limitations of this study, in open sandwich restorations, saliva contamination did not show an adverse effect on microleakage irrespective of dental materials tested. Glass hybrid ionomers and resin modified glass ionomers showed lower microleakage scores in gingival margins compared to high-viscous glass ionomer material in no contamination groups