Gruppe als pastoraler Handlungsort

Menschen sind ihr Leben lang auf Beziehungen und Bezugsgruppen angewiesen. Das Zweite Vatikanum und die Würzburger Synode haben den Bedeutungsgewinn von Gruppen innerhalb der Pastoral befördert. In der Folge konnten die Potenziale von Gruppen gut genutzt werden. Insbesondere wurden Gruppen als Räume erschlossen, in denen Gesellschaft verstanden und verändert werden konnte. Manchmal schätzte man die Möglichkeiten von Gruppen aber allzu euphorisch ein. Die gesamte Entwicklung ist rückblickend als Teil des „Psychobooms“ der 1970er- und 80er-Jahre einzuordnen. Heute sind Gruppenangebote aufgrund der Individualisierung der Gesellschaft und der Verplanung der Menschen weniger gefragt. Die Coronakrise hat diesen Rückgang verstärkt, aber die Ursachen lagen vor der Pandemie. Die Gruppe wird als Handlungsort erhalten bleiben, aber es ist wichtig, kritischer als bisher auf entsprechende Angebote zu schauen. Was wir heute brauchen, sind effektive Schutzmechanismen gegen Ideologisierung, Missbrauch und Gewalt. Wo dies gelingt, kann die Zugehörigkeit zu Gruppen eine passgenaue Lösung gegen die um sich greifende Einsamkeit sein. People depend on relationships and reference groups throughout their lives. The Second Vatican Council and the Würzburg Synod have promoted the increased importance of groups within pastoral ministry. As a result, the potentials of group have been well utilised. In particular, groups were opened up as spaces in which society could be understood and changed. Sometimes, however, the possibilities of groups were overly euphoric. In retrospect, the whole development can be classified as part of the "psychoboom" of the 1970s and 80s. Today, due to the individualisation of society and the scheduling of people, group offers are less in demand. The corona crisis has intensified this decline, but the causes predate the pandemic. The group will remain as a place of action, but it is important to look more critically than before at appropriate offers. What we need today are effective protective mechanisms against ideologization, abuse and violence. Where this succeeds, belonging to groups can be a fitting solution against the rampant loneliness

Cash or Non-Cash? Unveiling Ideators' Incentive Preferences in Crowdsourcing Contests

Even though research has repeatedly shown that non-cash incentives can be effective, cash incentives are the de facto standard in crowdsourcing contests. In this multi-study research, we quantify ideators' preferences for non-cash incentives and investigate how allowing ideators to self-select their preferred incentive -- offering ideators a choice between cash and non-cash incentives -- affects their creative performance. We further explore whether the market context of the organization hosting the contest -- social (non-profit) or monetary (for-profit) -- moderates incentive preferences and their effectiveness. We find that individuals exhibit heterogeneous incentive preferences and often prefer non-cash incentives, even in for-profit contexts. Offering ideators a choice of incentives can enhance creative performance. Market context moderates the effect of incentives, such that ideators who receive non-cash incentives in for-profit contexts tend to exert less effort. We show that heterogeneity of ideators' preferences (and the ability to satisfy diverse preferences with suitably diverse incentive options) is a critical boundary condition to realizing benefits from offering ideators a choice of incentives. We provide managers with guidance to design effective incentives by improving incentive-preference fit for ideators.Comment: Journal of Management Information Systems, forthcoming 202

Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering

Seaweeds emerge as promising third-generation renewable for sustainable bioproduction. In the present work, we valorized brown seaweed to produce l-lysine, the world's leading feed amino acid, using Corynebacterium glutamicum, which was streamlined by systems metabolic engineering. The mutant C. glutamicum SEA-1 served as a starting point for development because it produced small amounts of l-lysine from mannitol, a major seaweed sugar, because of the deletion of its arabitol repressor AtlR and its engineered l-lysine pathway. Starting from SEA-1, we systematically optimized the microbe to redirect excess NADH, formed on the sugar alcohol, towards NADPH, required for l-lysine synthesis. The mannitol dehydrogenase variant MtlD D75A, inspired by 3D protein homology modelling, partly generated NADPH during the oxidation of mannitol to fructose, leading to a 70% increased l-lysine yield in strain SEA-2C. Several rounds of strain engineering further increased NADPH supply and l-lysine production. The best strain, SEA-7, overexpressed the membrane-bound transhydrogenase pntAB together with codon-optimized gapN, encoding NADPH-dependent glyceraldehyde 3-phosphate dehydrogenase, and mak, encoding fructokinase. In a fed-batch process, SEA-7 produced 76 g L-1l-lysine from mannitol at a yield of 0.26 mol mol-1 and a maximum productivity of 2.1 g L-1 h-1. Finally, SEA-7 was integrated into seaweed valorization cascades. Aqua-cultured Laminaria digitata, a major seaweed for commercial alginate, was extracted and hydrolyzed enzymatically, followed by recovery and clean-up of pure alginate gum. The residual sugar-based mixture was converted to l-lysine at a yield of 0.27 C-mol C-mol-1 using SEA-7. Second, stems of the wild-harvested seaweed Durvillaea antarctica, obtained as waste during commercial processing of the blades for human consumption, were extracted using acid treatment. Fermentation of the hydrolysate using SEA-7 provided l-lysine at a yield of 0.40 C-mol C-mol-1. Our findings enable improvement of the efficiency of seaweed biorefineries using tailor-made C. glutamicum strains

Probing Teichoic Acid Genetics with Bioactive Molecules Reveals New Interactions among Diverse Processes in Bacterial Cell Wall Biogenesis

SummaryThe bacterial cell wall has been a celebrated target for antibiotics and holds real promise for the discovery of new antibacterial chemical matter. In addition to peptidoglycan, the walls of Gram-positive bacteria contain large amounts of the polymer teichoic acid, covalently attached to peptidoglycan. Recently, wall teichoic acid was shown to be essential to the proper morphology of Bacillus subtilis and an important virulence factor for Staphylococcus aureus. Additionally, recent studies have shown that the dispensability of genes encoding teichoic acid biosynthetic enzymes is paradoxical and complex. Here, we report on the discovery of a promoter (PywaC), which is sensitive to lesions in teichoic acid synthesis. Exploiting this promoter through a chemical-genetic approach, we revealed surprising interactions among undecaprenol, peptidoglycan, and teichoic acid biosynthesis that help explain the complexity of teichoic acid gene dispensability. Furthermore, the new reporter assay represents an exciting avenue for the discovery of antibacterial molecules

Multisensor hyperspectral imaging approach for the microchemical analysis of ultramarine blue pigments

This work presents a multisensor hyperspectral approach for the characterization of ultramarine blue, a valuable historical pigment, at the microscopic scale combining the information of four analytical techniques at the elemental and molecular levels. The hyperspectral images collected were combined in a single hypercube, where the pixels of the various spectral components are aligned on top of each other. Selected spectral descriptors have been defined to reduce data dimensionality before applying unsupervised chemometric data analysis approaches. Lazurite, responsible for the blue color of the pigment, was detected as the major mineral phase present in synthetic and good quality pigments. Impurities like pyrite were detected in lower quality samples, although the clear identification of other mineral phases with silicate basis was more difficult. There is no correlation between the spatial distribution of the bands arising in the Raman spectra of natural samples in the region 1200–1850 cm− 1 and any of the transition metals or rare earth elements (REE). With this information, the previous hypothesis (based on bulk analysis) attributing these bands to luminescence emissions due to impurities of these elements must be revised. We propose the consideration of CO2 molecules trapped in the cages of the aluminosilicate structure of sodalite-type. Additionally, correlation between certain Raman features and the combined presence of Ca, P, and REE, in particular Nd, was detected for the lowest quality pigment. Our results highlight the usefulness of fusing chemical images obtained via different imaging techniques to obtain relevant information on chemical structure and properties.Authors thank Research project BIA2017-87131-R from the Ministry of Economy and Competitiveness for financial support. M. Gonzalez‐Cabrera also acknowledges the Ministry of Education, Culture and Sports for a mobility Grant during her doctorate studies (FPU15/03119 fellowship). The authors also thank Hans Lohninger (Epina ImageLab) for technical support and Kremer Pigmente for the information about the commercial pigments

Cannabinoid Receptor 2 Signaling Does Not Modulate Atherogenesis in Mice

BACKGROUND:Strong evidence supports a protective role of the cannabinoid receptor 2 (CB(2)) in inflammation and atherosclerosis. However, direct proof of its involvement in lesion formation is lacking. Therefore, the present study aimed to characterize the role of the CB(2) receptor in Murine atherogenesis. METHODS AND FINDINGS:Low density lipoprotein receptor-deficient (LDLR(-/-)) mice subjected to intraperitoneal injections of the selective CB(2) receptor agonist JWH-133 or vehicle three times per week consumed high cholesterol diet (HCD) for 16 weeks. Surprisingly, intimal lesion size did not differ between both groups in sections of the aortic roots and arches, suggesting that CB(2) activation does not modulate atherogenesis in vivo. Plaque content of lipids, macrophages, smooth muscle cells, T cells, and collagen were also similar between both groups. Moreover, CB(2) (-/-)/LDLR(-/-) mice developed lesions of similar size containing more macrophages and lipids but similar amounts of smooth muscle cells and collagen fibers compared with CB(2) (+/+)/LDLR(-/-) controls. While JWH-133 treatment reduced intraperitoneal macrophage accumulation in thioglycollate-elicited peritonitis, neither genetic deficiency nor pharmacologic activation of the CB(2) receptor altered inflammatory cytokine expression in vivo or inflammatory cell adhesion in the flow chamber in vitro. CONCLUSION:Our study demonstrates that both activation and deletion of the CB(2) receptor do not relevantly modulate atherogenesis in mice. Our data do not challenge the multiple reports involving CB(2) in other inflammatory processes. However, in the context of atherosclerosis, CB(2) does not appear to be a suitable therapeutic target for reduction of the atherosclerotic plaque

Azimuthal anisotropy of charged jet production in root s(NN)=2.76 TeV Pb-Pb collisions

We present measurements of the azimuthal dependence of charged jet production in central and semi-central root s(NN) = 2.76 TeV Pb-Pb collisions with respect to the second harmonic event plane, quantified as nu(ch)(2) (jet). Jet finding is performed employing the anti-k(T) algorithm with a resolution parameter R = 0.2 using charged tracks from the ALICE tracking system. The contribution of the azimuthal anisotropy of the underlying event is taken into account event-by-event. The remaining (statistical) region-to-region fluctuations are removed on an ensemble basis by unfolding the jet spectra for different event plane orientations independently. Significant non-zero nu(ch)(2) (jet) is observed in semi-central collisions (30-50% centrality) for 20 <p(T)(ch) (jet) <90 GeV/c. The azimuthal dependence of the charged jet production is similar to the dependence observed for jets comprising both charged and neutral fragments, and compatible with measurements of the nu(2) of single charged particles at high p(T). Good agreement between the data and predictions from JEWEL, an event generator simulating parton shower evolution in the presence of a dense QCD medium, is found in semi-central collisions. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Peer reviewe