Geronimo Creek Outfall Replacement, The City of Seguin, Guadalupe County, Texas: An Intensive Cultural Resource Survey

The City of Seguin (City) is proposing to construct a new, replacement effluent outfall for the Geronimo Creek Wastewater Treatment Plant. The City also proposes to abandon the existing outfall structure and existing pipeline in place. The proposed project will involve the extension of an existing 24 inch (in) (61 centimeters [cm]) wastewater treatment plant effluent line for a length of 535 feet (ft.) (163 meters [m]). The line will terminate at a new outfall structure located on the east bank of Geronimo Creek, approximately 260 ft. (79 m) from its confluence with the Guadalupe River. Two new manholes will be constructed along the line at major bends in the line. Line depths will range from 9 to 28 ft. (2.7 to 8.5 m). A 40 ft. (12.2 m) wide permanent easement is proposed for the line, along with a 30 ft. (9.1 m) wide temporary easementfor construction purposes. The extent of the outfallstructure should be fully within the 40 ft. (12.2 m) wide easement. The area of potential effect (APE) is defined as an area measuring 535 ft. (163 m) in length, and 70 ft. (21.3 m) in width for a total of 0.85 acres, and generally follows the eastern bank of Geronimo Creek. The Texas Historical Commission (THC) reviewed the proposed development plans and determined that a cultural resource survey was necessary for this City proposed project as the area has potential for cultural resources. The City contracted with TRC Environmental Corporation (TRC) of Austin to conduct the intensive cultural resource survey of their proposed undertaking/APE. TRC archeologists consulted the THC Archeological Sites Atlas (THC Atlas), a database that contains previously documented cultural resource sites and locations of previously conducted archeological surveys, prior to the field investigations. A 1 mi. (1.6 km) radius search around the APE revealed one previously recorded cultural resource site, 41GU21. This prehistoric site is on a high terrace just west of Geronimo Creek and has been extensively disturbed during modern housing construction and is considered ineligible for inclusion in the National Register of Historic Places (NRHP). No previous cultural resources surveys have been conducted in the vicinity of the APE. Southwest of the APE and beyond the 1 mi. (1.6 km) radius was a Texas Department of Transportation survey conducted in 2007 by Moore Consulting, Inc. along Farm-to-Market road 477. No cultural resources were recorded in that linear investigation. On October 14 and 15, 2014, Mike Quigg, a TRC archeologists of the Planning, Permitting, and Licensing Practice of TRC’s office in Austin, conducted an intensive cultural resource survey along the 535 ft. (163 m) length of proposed APE. This included a 100 percent pedestrian survey, plus the mechanical excavation of four backhoe trenches (BT) within the APE to determine the presence/absence of any potential cultural resources within the APE. One deeply buried prehistoric site of potential significance was identified in two trenches (BTs 1 and 2) on the upper terrace along the APE. No cultural materials were recovered from two trenches (BTs 3 and 4) dug into the APE in the lower terrace. No historic structures were in the proposed APE. TRC recommends avoidance of the potentially significant, deep stratified prehistoric site. If the site cannot be avoided then TRC recommends further testing to assess the sites significance, and the potential to yield significant information important in prehistory, either locally, regionally or nationally as per its legal obligations under existing state guidelines. The City of Seguin reviewed the draft report, the archeological findings, and recommendations by TRC archeologists, and sought an alternate route for the proposed sewer line to avoid impact to cultural resource site 41GU168. The City and its engineers agreed to an alternate reroute satisfactory with the landowner that avoids the upper terrace The City of Seguin reviewed the draft report, the archeological findings, and recommendations by TRC archeologists, and sought an alternate route for the proposed sewer line to avoid impact to cultural resource site 41GU168. The City and its engineers agreed to an alternate reroute satisfactory with the landowner that avoids the upper terrace and the archeological site altogether. This proposed reroute successfully avoids and preserves deeply buried prehistoric site 41GU168 in place. TRC now recommends no further cultural resource investigation following the new proposed sewer line location

Cultural Resource Survey of the U.S. Highway 67 Water Improvement Project, City of Presidio, Presidio County, Texas

The City of Presidio is proposing to upgrade their water distribution system, provide services to the Colonia of Las Pampas, north of Presidio, and improve the overall water system reliability to accommodate these additional demands. Following a review of the proposed undertaking, the Texas Historic Commission (THC) recommended that a cultural resource survey be performed (THC letter dated October 21, 2015). To meet its responsibilities under existing State and Federal statutes, the City contracted TRC Environmental Corporation (TRC) of Austin to conduct the necessary cultural resource survey. Subsequently, TRC archeologists submitted a Texas Antiquities Permit Application to the THC, and Antiquities Permit #7722 was issued to archeologist Benjamin G. Bury, who served as Principal Investigator. The Area of Potential Effect (APE) consists of approximately 8.18 acres and includes the existing Texas Department of Transportation (TxDOT) ROW along U.S. 67 and two 0.02 acre parcels adjacent to the ROW that are currently privately owned, but will be acquired by TxDOT as new ROW easements prior to construction. The project will be funded by the Border Environment Cooperation Commission (BECC). Given the involvement of the U. S. Environmental Protection Agency (USEPA) with the BECC, the USEPA is the lead federal agency for this effort. Fieldwork was conducted on July 26th and July 27th, 2016. The field effort consisted of a systematic pedestrian survey at 15 m (50 ft.) intervals across the APE. Cultural resources documented during the survey included one historic site (41PS1220) and three isolated core reduction flakes that were widely separated and not associated with any other cultural materials. Site 41PS1220 consists of mid-to late-20th century trash left from informal dumping along U.S. 67. The dumping ground has been graded smooth in the last 30 years. Consequently, site materials are in a secondary context. Given these disturbances, TRC recommends that site 41PS1220 is not eligible for inclusion in the National Register of Historic Places (NRHP) or qualified for nomination as a State Antiquities Landmark (SAL). Additionally, one previously recorded site (41PS346) recorded within the ROW in 1976 was not relocated during the investigation, and has likely been significantly redeposited during previous road maintenance activities. TRC recommends that the portion of site 41PS346 within the U.S. 67 ROW is not eligible for inclusion in the NRHP or qualified for nomination as a SAL. Therefore, TRC recommends the project proceed as planned

Big Hole (41TV2161): Two Stratigraphically Isolated Middle Holocene Components in Travis County, Texas Volume I

During April and May 2006, an archeological team from the Cultural Resources Section of the Planning, Permitting and Licensing Practice of TRC Environmental Corporation’s (TRC) Austin office conducted geoarcheological documentation and data recovery excavations at prehistoric site 41TV2161 (CSJ: 0440-06-006). Investigations were restricted to a 70 centimeter (cm) thick target zone between ca. 220 and 290 cm below surface (bs) on the western side of site 41TV2161 – the Big Hole site in eastern Travis County, Texas. This cultural investigation was necessary under the requirements of Section 106 of the National Historic Preservation Act (NHPA), the implementing regulations of 36CRF Part 800 and the Antiquities Code of Texas (Texas Natural Resource Code, Title 9, Chapter 191 as amended) to recover a sample of the significant cultural materials prior to destruction by planned construction of State Highway 130 (SH 130). The latter by a private construction firm – Lone Star Infrastructure. This necessary data recovery was for Texas Department of Transportation (TxDOT), Environmental (ENV) Affairs Division under a Scientific Services Contract No. 577XXSA003 (Work Authorization No. 57701SA003). Over the years since the original award, multiple work authorizations between TxDOT and TRC were implemented and completed towards specific aspects of the analyses and reporting. The final analyses and report were conducted under contract 57-3XXSA004 (Work Authorization 57-311SA004). All work was under Texas Antiquities Committee Permit No. 4064 issued by the Texas Historical Commission (THC) to J. Michael Quigg. Initially, an archeological crew from Hicks & Company encountered site 41TV2161 during an intensive cultural resource inventory conducted south of Pearce Lane along the planned construction zone of SH 130 in the fall of 2005. Following the initial site discovery, archeologists expanded their investigations to the west across the SH 130 right-of-way, and completed excavation of 10 backhoe trenches, 13 shovel tests, and 11 test units at site 41TV2161. The investigations encountered at least seven buried cultural features and 1,034 artifacts, some in relatively good context. The survey and testing report to TxDOT presented their findings and recommendations (Campbell et al. 2006). The ENV Affairs Division of TxDOT and the THC reviewed the initial findings and recommendations, and determined site 41TV2161 was eligible for listing on the National Register of Historic Places and as State Antiquities Landmark as the proposed roadway development was to directly impact this important site and further excavations were required. Subsequently, TRC archeologists led by Paul Matchen (Project Archeologist) and J. Michael Quigg (Principal Investigator) initiated data recovery excavations through the mechanical-removal of between 220 and 250 cm of sediment from a 30-by-40 meter (m) block area (roughly 3,000 m3). This was conducted to allow hand-excavations to start just above the deeply buried, roughly 70 cm thick targeted zone of cultural material. Mechanical stripping by Lone Star Infrastructure staff created a large hole with an irregular bottom that varied between 220 and 260 cmbs. To locate specific areas to initiate hand-excavations within the mechanically stripped area, a geophysical survey that employed ground penetrating radar (GPR) was conducted by Tiffany Osburn then with Geo-Marine in Plano, Texas. Over a dozen electronic anomalies were detected through the GPR investigation. Following processing, data filtering, and assessment, Osburn identified and ranked the anomalies for investigation. The highest ranked anomalies (1 through 8) were thought to have the greatest potential to represent cultural features. Anomalies 1 through 6 were selected and targeted through hand-excavations of 1-by-1 m units that formed continuous excavation blocks of various sizes. Blocks were designated A, B, C, D, E, and F. The type, nature, quantity, and context of encountered cultural materials in each block led the direction and expansion of each excavation block as needed. In total, TRC archeologists hand-excavated 38.5 m3 (150 m2) from a vertically narrow target zone within this deep, multicomponent and stratified prehistoric site. Hand-excavation in the two largest Blocks, B and D (51 m2 and 62 m2 respectively), revealed two vertically separate cultural components between roughly 220 and 290 cmbs. The younger component was restricted to Block B and yielded a Bell/Andice point and point base, plus a complete Big Sandy point. These points were associated with at least eight small burned rock features, one cluster of ground stone tools, limited quantities of lithic debitage, few formal chipped and ground stone tools, and a rare vertebrate faunal assemblage. Roughly 20 to 25 cm below the Bell/Andice component in Block B and across Block D was a component identified by a single corner-notched Martindale dart point. This point was associated with a scattered burned rocks, three charcoal stained hearth features, scattered animal, bird, and fish bones, mussel shells, and less than a dozen formal chipped and ground stone tools. Both identified components contained cultural materials in good stratigraphic context with high spatial integrity. Significant, both were radiocarbon dated by multiple charcoal samples to a narrow 200-year period between 5250 and 5450 B.P. during the middle Holocene. With exception of the well-preserved faunal assemblages, perishable materials were poorly preserved in the moist silty clay loam. Charcoal lacked structure and was reduced to dark stains. Microfossils (e.g., phytoliths and starch gains) were present, although in very limited numbers and deteriorated conditions. The four much smaller Blocks (A, C, E, and F) yielded various quantities of cultural material and features, but these blocks also lacked sufficient charcoal dates and diagnostic artifacts Those artifacts and samples were left unassigned and analyzed separately from the Bell/Andice and Martindale components. The two well-defined components in Blocks B and D are the focus of this technical report. The components provide very significant data towards understanding rare and poorly understood hunter-gatherer populations during late stages of the Altithermal climate period. This final report builds upon the interim report submitted to TxDOT (Quigg et al. 2007) that briefly described the methods, excavations, preliminary findings, initial results from six feasibility studies, and proposed an initial research design for data analyses. Context and integrity of the cultural materials in the two identified components was excellent. This rare circumstance combined with detailed artifact analyses, solid documentation of their ages through multiple radiocarbon dates, and multidisciplinary approach to analyses, allowed significant insights and contributions concerning the two populations involved. Results provide a greater understanding of human behaviors during a rarely identified time in Texas Prehistory. The cultural materials and various collected samples were temporarily curated at TRC’s Austin laboratory. Following completion of analyses and acceptance of this final report, the artifacts, paper records, photographs, and electronic database were permanently curated at the Center for Archaeological Studies (CAS) at Texas State University in San Marcos

GENESIS: Co-location of Geodetic Techniques in Space

Improving and homogenizing time and space reference systems on Earth and, more directly, realizing the Terrestrial Reference Frame (TRF) with an accuracy of 1mm and a long-term stability of 0.1mm/year are relevant for many scientific and societal endeavors. The knowledge of the TRF is fundamental for Earth and navigation sciences. For instance, quantifying sea level change strongly depends on an accurate determination of the geocenter motion but also of the positions of continental and island reference stations, as well as the ground stations of tracking networks. Also, numerous applications in geophysics require absolute millimeter precision from the reference frame, as for example monitoring tectonic motion or crustal deformation for predicting natural hazards. The TRF accuracy to be achieved represents the consensus of various authorities which has enunciated geodesy requirements for Earth sciences. Today we are still far from these ambitious accuracy and stability goals for the realization of the TRF. However, a combination and co-location of all four space geodetic techniques on one satellite platform can significantly contribute to achieving these goals. This is the purpose of the GENESIS mission, proposed as a component of the FutureNAV program of the European Space Agency. The GENESIS platform will be a dynamic space geodetic observatory carrying all the geodetic instruments referenced to one another through carefully calibrated space ties. The co-location of the techniques in space will solve the inconsistencies and biases between the different geodetic techniques in order to reach the TRF accuracy and stability goals endorsed by the various international authorities and the scientific community. The purpose of this white paper is to review the state-of-the-art and explain the benefits of the GENESIS mission in Earth sciences, navigation sciences and metrology.Comment: 31 pages, 9 figures, submitted to Earth, Planets and Space (EPS

Allele-Specific HLA Loss and Immune Escape in Lung Cancer Evolution

Immune evasion is a hallmark of cancer. Losing the ability to present neoantigens through human leukocyte antigen (HLA) loss may facilitate immune evasion. However, the polymorphic nature of the locus has precluded accurate HLA copy-number analysis. Here, we present loss of heterozygosity in human leukocyte antigen (LOHHLA), a computational tool to determine HLA allele-specific copy number from sequencing data. Using LOHHLA, we find that HLA LOH occurs in 40% of non-small-cell lung cancers (NSCLCs) and is associated with a high subclonal neoantigen burden, APOBEC-mediated mutagenesis, upregulation of cytolytic activity, and PD-L1 positivity. The focal nature of HLA LOH alterations, their subclonal frequencies, enrichment in metastatic sites, and occurrence as parallel events suggests that HLA LOH is an immune escape mechanism that is subject to strong microenvironmental selection pressures later in tumor evolution. Characterizing HLA LOH with LOHHLA refines neoantigen prediction and may have implications for our understanding of resistance mechanisms and immunotherapeutic approaches targeting neoantigens. Video Abstract [Figure presented] Development of the bioinformatics tool LOHHLA allows precise measurement of allele-specific HLA copy number, improves the accuracy in neoantigen prediction, and uncovers insights into how immune escape contributes to tumor evolution in non-small-cell lung cancer

Fc-Optimized Anti-CD25 Depletes Tumor-Infiltrating Regulatory T Cells and Synergizes with PD-1 Blockade to Eradicate Established Tumors

CD25 is expressed at high levels on regulatory T (Treg) cells and was initially proposed as a target for cancer immunotherapy. However, anti-CD25 antibodies have displayed limited activity against established tumors. We demonstrated that CD25 expression is largely restricted to tumor-infiltrating Treg cells in mice and humans. While existing anti-CD25 antibodies were observed to deplete Treg cells in the periphery, upregulation of the inhibitory Fc gamma receptor (FcγR) IIb at the tumor site prevented intra-tumoral Treg cell depletion, which may underlie the lack of anti-tumor activity previously observed in pre-clinical models. Use of an anti-CD25 antibody with enhanced binding to activating FcγRs led to effective depletion of tumor-infiltrating Treg cells, increased effector to Treg cell ratios, and improved control of established tumors. Combination with anti-programmed cell death protein-1 antibodies promoted complete tumor rejection, demonstrating the relevance of CD25 as a therapeutic target and promising substrate for future combination approaches in immune-oncology

Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution.

The early detection of relapse following primary surgery for non-small-cell lung cancer and the characterization of emerging subclones, which seed metastatic sites, might offer new therapeutic approaches for limiting tumour recurrence. The ability to track the evolutionary dynamics of early-stage lung cancer non-invasively in circulating tumour DNA (ctDNA) has not yet been demonstrated. Here we use a tumour-specific phylogenetic approach to profile the ctDNA of the first 100 TRACERx (Tracking Non-Small-Cell Lung Cancer Evolution Through Therapy (Rx)) study participants, including one patient who was also recruited to the PEACE (Posthumous Evaluation of Advanced Cancer Environment) post-mortem study. We identify independent predictors of ctDNA release and analyse the tumour-volume detection limit. Through blinded profiling of postoperative plasma, we observe evidence of adjuvant chemotherapy resistance and identify patients who are very likely to experience recurrence of their lung cancer. Finally, we show that phylogenetic ctDNA profiling tracks the subclonal nature of lung cancer relapse and metastasis, providing a new approach for ctDNA-driven therapeutic studies

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe

Reconstruction of signal amplitudes in the CMS electromagnetic calorimeter in the presence of overlapping proton-proton interactions

A template fitting technique for reconstructing the amplitude of signals produced by the lead tungstate crystals of the CMS electromagnetic calorimeter is described. This novel approach is designed to suppress the contribution to the signal of the increased number of out-of-time interactions per beam crossing following the reduction of the accelerator bunch spacing from 50 to 25 ns at the start of Run 2 of the LHC. Execution of the algorithm is sufficiently fast for it to be employed in the CMS high-level trigger. It is also used in the offline event reconstruction. Results obtained from simulations and from Run 2 collision data (2015-2018) demonstrate a substantial improvement in the energy resolution of the calorimeter over a range of energies extending from a few GeV to several tens of GeV.Peer reviewe

Observation of the Production of Three Massive Gauge Bosons at root s=13 TeV

The first observation is reported of the combined production of three massive gauge bosons (VVV with V = W, Z) in proton-proton collisions at a center-of-mass energy of 13 TeV. The analysis is based on a data sample recorded by the CMS experiment at the CERN LHC corresponding to an integrated luminosity of 137 fb(-1). The searches for individualWWW, WWZ, WZZ, and ZZZ production are performed in final states with three, four, five, and six leptons (electrons or muons), or with two same-sign leptons plus one or two jets. The observed (expected) significance of the combinedVVV production signal is 5.7 (5.9) standard deviations and the corresponding measured cross section relative to the standard model prediction is 1.02(-0.23)(+0.26). The significances of the individual WWW and WWZ production are 3.3 and 3.4 standard deviations, respectively. Measured production cross sections for the individual triboson processes are also reported