Stoquasticity in circuit QED

We analyze whether circuit-QED Hamiltonians are stoquastic focusing on systems of coupled flux qubits: we show that scalable sign-problem free path integral Monte Carlo simulations can typically be performed for such systems. Despite this, we corroborate the recent finding [arXiv:1903.06139] that an effective, non-stoquastic qubit Hamiltonian can emerge in a system of capacitively coupled flux qubits. We find that if the capacitive coupling is sufficiently small, this non-stoquasticity of the effective qubit Hamiltonian can be avoided if we perform a canonical transformation prior to projecting onto an effective qubit Hamiltonian. Our results shed light on the power of circuit-QED Hamiltonians for the use of quantum adiabatic computation and the subtlety of finding a representation which cures the sign problem in these system

Homological Quantum Rotor Codes: Logical Qubits from Torsion

We formally define homological quantum rotor codes which use multiple quantum rotors to encode logical information. These codes generalize homological or CSS quantum codes for qubits or qudits, as well as linear oscillator codes which encode logical oscillators. Unlike for qubits or oscillators, homological quantum rotor codes allow one to encode both logical rotors and logical qudits, depending on the homology of the underlying chain complex. In particular, such a code based on the chain complex obtained from tessellating the real projective plane or a M\"{o}bius strip encodes a qubit. We discuss the distance scaling for such codes which can be more subtle than in the qubit case due to the concept of logical operator spreading by continuous stabilizer phase-shifts. We give constructions of homological quantum rotor codes based on 2D and 3D manifolds as well as products of chain complexes. Superconducting devices being composed of islands with integer Cooper pair charges could form a natural hardware platform for realizing these codes: we show that the $0$-$\pi$-qubit as well as Kitaev's current-mirror qubit -- also known as the M\"{o}bius strip qubit -- are indeed small examples of such codes and discuss possible extensions.Comment: 47 pages, 10 figures, 2 table

Analisi della variabilità genetica in una popolazione ovina di razza massese e studio di associazione con parametri di qualità del latte

RIASSUNTO Il presente lavoro ha come obiettivo la ricerca di associazioni tra marcatori microsatellite e la composizione chimica del latte. È stato condotto uno studio su 68 pecore di razza Massese provenienti da un allevamento toscano. A partire da latte fresco, sono stati valutati: composizione chimica standard, contenuto in caseina e frazioni caseiniche, proteine seriche, pH e parametri reologici. I capi sono stati sottoposti a genotipizzazione impiegando 17 marcatori microsatellite; sono, quindi, stati calcolati i valori di similarità genetica tra individui ed alcuni parametri genetici classici. Il numero medio di alleli per locus è risultato pari a 7,18 e l’eterozigosità osservata presentava variazioni tra 0,403 e 0,867 (media 0,677). La similarità genetica tra individui era 0,460. Cinque marcatori (BM8124, CSN3, BM1258, BMS468 e TGLA387) hanno mostrato una deviazione significativa dalle proporzioni di Hardy- Weinberg. Lo studio ha messo in evidenza alcuni microsatelliti con alleli significativamente associati a caratteri di composizione del latte (P<0,01). In particolare, la significatività più alta (P<0,001) è stata osservata per l’associazione dell’allele 2 del marcatore OIFNG con la a-lattoalbumina, dell’allele 10 del marcatore BL4 con il livello di immunoglobuline, dell’allele 2 del marcatore BMC1009 con il contenuto di grasso e dell’allele 9 del marcatore ILSTS42 con il parametro a30. Sono necessari ulteriori approfondimenti al fine di convalidare i risultati preliminari ottenuti nel presente lavoro e si rende necessaria l’estensione dello studio ad un numero maggiore di soggetti e la tipizzazione di un numero maggiore di loci, principalmente sui cromosomi 3 e 20 dove mappano i marcatori risultati più interessanti nella presente analisi. SUMMARY Current research aims to establish statistical associations between DNA microsatellites and milk chemical composition. Atrial was carried out on 68 Massese ewes reared in a farm of the Tuscany. The following parameters were evaluated on fresh milk: standard chemical composition, casein and its fractions, whey proteins, pH and rheological parameters. Animals were genotyped at 17 microsatellite loci. Genetic similarities among individuals and classical genetic parameters were evaluated. For each locus, average values of considered parameters were calculated in all the subjects carrying a given allele; these were compared statistically with the average values of subjects not carrying the allele, and the significance of the difference was estimated. The average number of alleles per locus resulted 7.18 and the observed heterozygosity ranged from 0.403 to 0.867 (0.677 medium value). The genetic similarity among individuals was 0.460. Five markers pointed out a significant deviation from the Hardy-Weinberg proportions (BM8124, CSN3, BM1258, BMS468 and TGLA387). The study revealed several microsatellites with alleles significantly linked to milk composition traits (P<0.01). In particular the highest significance (P<0.001) has been found for the allele 2 of OIFNG marker with a-lactoalbumin, for the allele 10 of the BL4 marker with immunoglobulins, for the allele 2 of BMC1009 with fat, and for allele 9 of ILSTS42 with a30. Further analyses are needed to validate these preliminary results, in particular increasing the number of subjects and of typed loci above all on the chromosomes 3 and 20 where the more interesting markers map

Microwave-activated gates between a fluxonium and a transmon qubit

We propose and analyze two types of microwave-activated gates between a fluxonium and a transmon qubit, namely a cross-resonance (CR) and a CPHASE gate. The large frequency difference between a transmon and a fluxonium makes the realization of a two-qubit gate challenging. For a medium-frequency fluxonium qubit, the transmon-fluxonium system allows for a cross-resonance effect mediated by the higher levels of the fluxonium over a wide range of transmon frequencies. This allows one to realize the cross-resonance gate by driving the fluxonium at the transmon frequency, mitigating typical problems of the cross-resonance gate in transmon-transmon chips related to frequency targeting and residual ZZ coupling. However, when the fundamental frequency of the fluxonium enters the low-frequency regime below 100 MHz, the cross-resonance effect decreases leading to long gate times. For this range of parameters, a fast microwave CPHASE gate can be implemented using the higher levels of the fluxonium. In both cases, we perform numerical simulations of the gate showing that a gate fidelity above 99% can be obtained with gate times between 100 and 300 ns. Next to a detailed gate analysis, we perform a study of chip yield for a surface code lattice of fluxonia and transmons interacting via the proposed cross-resonance gate. We find a much better yield as compared to a transmon-only architecture with the cross-resonance gate as native two-qubit gate

Adipokines as Possible New Predictors of Cardiovascular Diseases: A Case Control Study

Background and Aims. The secretion of several adipocytokines, such as adiponectin, retinol-binding protein 4 (RBP4), adipocyte fatty acid binding protein (aFABP), and visfatin, is altered in subjects with abdominal adiposity; these endocrine alterations could contribute to increased cardiovascular risk. The aim of the study was to assess the relationship among adiponectin, RBP4, aFABP, and visfatin, and incident cardiovascular disease. Methods and Results. A case-control study, nested within a prospective cohort, on 2945 subjects enrolled for a diabetes screening program was performed. We studied 18 patients with incident fatal or nonfatal IHD (Ischemic Heart Disease) or CVD (Cerebrovascular Disease), compared with 18 matched control subjects. Circulating adiponectin levels were significantly lower in cases of IHD with respect to controls. Circulating RBP4 levels were significantly increased in CVD and decreased in IHD with respect to controls. Circulating aFABP4 levels were significantly increased in CVD, while no difference was associated with IHD. Circulating visfatin levels were significantly lower in cases of both CVD and IHD with respect to controls, while no difference was associated with CVD. Conclusions. The present study confirms that low adiponectin is associated with increased incidents of IHD, but not CVD, and suggests, for the first time, a major effect of visfatin, aFABP, and RBP4 in the development of cardiovascular disease

Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen

Antioxidant supplementation has been proposed as a new strategy to improve the long-term preservation of semen. The aim of this study was to evaluate the effect of Maca supplementation of semen extender on quality-related canine semen parameters during cooling. Ejaculates from nine dogs were cooled for 7 days in the absence (control group) or in the presence of 10, 20 and 50 μL/mL of an aqueous extract of Maca. Sperm were evaluated for sperm viability, motility, DNA fragmentation and lipid peroxidation after 3 h, 24 h, 4 days and 7 days of storage. The addition of 10 μL/mL of Maca preserved sperm DNA and plasma membrane integrity at 3 h and increased sperm curvilinear velocity after 24 h. Treatment with 20 and 50 μL/mL of Maca increased the percentage of hyperactivated sperm after 3 h. Moreover, semen treated with 20 μL/mL of Maca decreased lipid peroxidation at 24 h. A significant reduction of sperm DNA and plasma membrane integrity as well as of kinetics parameters between 3 and 24 h of refrigerated storage with the higher concentration tested was observed. Although Maca was not able to protect canine semen with extended refrigeration storage time, it increased hyperactivation and preserved DNA integrity in short-term storage

pH dependent binding in de novo hetero bimetallic coiled coils

Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(II) and Tb(III) to the two distinct sites within this coiled coil, and offers the opportunity to “activate” metal binding sites within complex multi metallo and multi-functional designs

No evidence for cardiac dysfunction in Kif6 mutant mice.

A KIF6 variant in man has been reported to be associated with adverse cardiovascular outcomes after myocardial infarction. No clear biological or physiological data exist for Kif6. We sought to investigate the impact of a deleterious KIF6 mutation on cardiac function in mice. Kif6 mutant mice were generated and verified. Cardiac function was assessed by serial echocardiography at baseline, after ageing and after exercise. Lipid levels were also measured. No discernable adverse lipid or cardiac phenotype was detected in Kif6 mutant mice. These data suggest that dysfunction of Kif6 is linked to other more complex biological/biochemical parameters or is unlikely to be of material consequence in cardiac function

A conserved loop-wedge motif moderates reaction site search and recognition by FEN1

DNA replication and repair frequently involve intermediate two-way junction structures with overhangs, or flaps, that must be promptly removed; a task performed by the essential enzyme flap endonuclease 1 (FEN1). We demonstrate a functional relationship between two intrinsically disordered regions of the FEN1 protein, which recognise opposing sides of the junction and order in response to the requisite substrate. Our results inform a model in which short-range translocation of FEN1 on DNA facilitates search for the annealed 3′‑terminus of a primer strand, which is recognised by breaking the terminal base pair to generate a substrate with a single nucleotide 3′‑flap. This recognition event allosterically signals hydrolytic removal of the 5′-flap through reaction in the opposing junction duplex, by controlling access of the scissile phosphate diester to the active site. The recognition process relies on a highly-conserved ‘wedge’ residue located on a mobile loop that orders to bind the newly-unpaired base. The unanticipated ‘loop–wedge’ mechanism exerts control over substrate selection, rate of reaction and reaction site precision, and shares features with other enzymes that recognise irregular DNA structures. These new findings reveal how FEN1 precisely couples 3′-flap verification to function

Nature's lessons in design: nanomachines to scaffold, remodel and shape membrane compartments.

Compartmentalisation of cellular processes is fundamental to regulation of metabolism in Eukaryotic organisms and is primarily provided by membrane-bound organelles. These organelles are dynamic structures whose membrane barriers are continually shaped, remodelled and scaffolded by a rich variety of highly sophisticated protein complexes. Towards the goal of bottom-up assembly of compartmentalised protocells in synthetic biology, we believe it will be important to harness and reconstitute the membrane shaping and sculpting characteristics of natural cells. We review different in vitro membrane models and how biophysical investigations of minimal systems combined with appropriate theoretical modelling have been used to gain new insights into the intricate mechanisms of these membrane nanomachines, paying particular attention to proteins involved in membrane fusion, fission and cytoskeletal scaffolding processes. We argue that minimal machineries need to be developed and optimised for employment in artificial protocell systems rather than the complex environs of a living organism. Thus, well-characterised minimal components might be predictably combined into functional, compartmentalised protocellular materials that can be engineered for wide-ranging applications