Candida carriage in the alimentary tract of liver transplant candidates

Thirty randomly selected patients with advanced chronic liver disease, which had been evaluated for possible liver transplantation, were sampled endoscopically at 7 alimentary tract locations to assess the frequency and amount of Candida carriage. Eightyone percent (127/156) of the samples obtained contained Candida and 53% (82/156) yielded high counts (> 300 CFU/ml). The most predominant Candida species isolated at each site was Candida albicana, which accounted for 103 (64%) of the 160 fungal isolates. The other Candida species isolated included C tropicalis 30 (19%), C krusei 16 (10%), and C glabrata 11 (7%), Although the number of sites at which yeast was present and the quantities of yeast at each site varied widely among the patients studied, 100% of the patients had Candida in at least one site of the gastrointestinal tract. Eighty-six percent (24/28) of the duodenal aspirates contained Candida and 50% (14/28) of the duodenal samples contained greater than 300 CFU/ml. A positive culture from the stomach was a reliable predictor of the presence of Candida in the duodenum (P=0.0001), but a positive culture at no other site readily predicted the presence of Candida at yet another site. Importantly, there was no correlation between the presence or absence of Candida in either oral or rectal swabs and colonization at other anatomic sites within the gastrointestinal tract, These findings are important in liver transplantation, particularly in those cases in which the bowel has been opened to create a choledochojejunostomy anastomosis. The operative attempts to reduce gastrointestinal fungal carriage using oral antifungal agents may be justified before liver transplantation in an effort to lower the risk of posttransplantation fungal infections, particularly in those patients expected to have a Roux-en-Y choledochojejunostomy biliary reconstruction. © 1994 by Williams and Wilkins

Bronchoscopy for Removal of Aspirated Tracheobronchial Foreign Bodies at Kenyatta National Hospital, in Kenya

Background: This study was aimed at defining the presentation, morbidity and mortality following inhalation and subsequent removal of  tracheobronchial foreign body using a rigid bronchoscope.Methods: This was a retrospective, cross-sectional study undertaken in the Ear Nose and Throat-Head and Neck Surgery Unit of Kenyatta National Hospital. The study population consisted of patients with brochoscopically proven tracheobronchial foreign body. Case notes of 107 consecutive admissions with brochoscopically verified tracheobronchial foreign bodieswere analyzed for age, sex, time interval between inhalation and removal, duration of stay in hospital, complications and mortality.Results: The vast majority of patients were below 3 years of age and 63% of them presented within 3 days after onset of symptoms. The right bronchus was the most frequent destination of inhaled foreign bodies (50.5%) followed by the left bronchus (17.8%) and carina (14.2%) inthat order. The turnover of patients with inhaled foreign bodies was high with 86.9% being discharged within 6 days after surgery. The bronchoscopic procedure was uneventful in 76.6% of patients. Repeat bronchoscopy was performed in three patients with one undergoing eventual thoracotomy.Intraoperative cardiac arrest occurred in 5 cases (4.6%) with successful resuscitation in 2 and mortality in three (2.7%) % cases.Conclusion: Bronchoscopic removal of inhaled foreign bodies remains one of the riskiest otolaryngologic emergencies in Kenyatta National Hospital partly because of involvement of the life sustaining airway and partly because of lack of ideal equipment and adequate expertise. The need for hands on experience and close cooperation and familiarity between theanaesthesiologist and the bonchoscopist is emphasized

Use of indigenous knowledge in the management of field and storage pests around Lake Victoria basin in Tanzania

Agriculture in Lake Victoria basin (LVB) in Tanzania is predominantly subsistence and is characterised by perennial food deficits, cyclic famines and poverty prompted largely by unreliable rainfall patterns, declining soil fertility and food grains pests and diseases. The pest problem is more pronounced as farmers are yet to fully integrate synthetic pesticides into their insect pest management systems due to subsistence nature of production and high poverty levels that make them rely on indigenous knowledge (IK) systems to meet their needs. The survey was conducted to document farmers’ IK on management of key field and storage insect pests in Magu and Misungwi districts in the LVB, Tanzania. Major crops grown were maize, rice, sorghum, finger millet, bean, groundnut, cowpea, green gram, brassicas, chicken pea, cassava, sweet potato, cotton and vegetables. Crops were mainly infested by Busseola fusca (Lepidoptera: Noctuidae), Spodoptera spp (Lepidoptera: Noctuidae), Agrotis spp (Lepidoptera: Noctuidae), Maruca vitrata (Lepidoptera: Crambidae), Rhopalosiphum maidis (Homoptera: Aphididae), Aphis fabae (Hemiptera: Aphididae), and grasshoppers in field and Stophilus spp (Coleoptera: Curculionidae), Prostephanus truncates (Coleoptera: Bostrichidae), Tribolium spp (Coleoptera: Tenebrionidae), Bruchus rufimanus (Coleoptera; Bruchidae), Rhyzopertha dominica (Coleoptera: Bostrichidae) and rodents on storage. IK based control methods used by farmers ranged from animal by-products (cow’s urine and dung), plant parts (Azadirachta indica (Meliaceae),Tephrosia vogelii (Fabaceae), Tamarindus indica (Fabaceae), Aloe spp (Asphodelaceae), red pepper, Capsicum spp (Solanaceae), Nicotiana tabasum (Solanaceae) to ash (general and specific) in the field. They also used neem, Chenopodium opulifolium (Chenopodiaceae), Ocimum suave (Labiatae), Senna siamea (Fabaceae or Caesalpinioideae), tobacco and Eucalyptus spp (Myrtaceae) and plant by-products (rice husks, ash from rice husks and red maize cobs and general ash) to control storage pests. Most of these products were used together with one or two others in different formulation mixtures. However, the formulations had variable amount taken during preparation, crop/ crop product treated, preparation times, modes and rates of application. Research is needed to unveil the amount for mixing, appropriate treatment, and application rate to ensure optimum concentration for specific pest. To ensure quality and safety, biosafety and quality studies are required for quality assessment of resulting product for human health. For understanding of active compounds in the formulations, chemical composition analysis of properly prepared solutions is required. Key words: Field and storage pests, indigenous knowledge, Tanzania, botanical formulation, Lake Victoria basin

Fourier Growth of Structured ??-Polynomials and Applications

We analyze the Fourier growth, i.e. the L? Fourier weight at level k (denoted L_{1,k}), of various well-studied classes of "structured" m F?-polynomials. This study is motivated by applications in pseudorandomness, in particular recent results and conjectures due to [Chattopadhyay et al., 2019; Chattopadhyay et al., 2019; Eshan Chattopadhyay et al., 2020] which show that upper bounds on Fourier growth (even at level k = 2) give unconditional pseudorandom generators. Our main structural results on Fourier growth are as follows: - We show that any symmetric degree-d m F?-polynomial p has L_{1,k}(p) ? Pr [p = 1] ? O(d)^k. This quadratically strengthens an earlier bound that was implicit in [Omer Reingold et al., 2013]. - We show that any read-? degree-d m F?-polynomial p has L_{1,k}(p) ? Pr [p = 1] ? (k ? d)^{O(k)}. - We establish a composition theorem which gives L_{1,k} bounds on disjoint compositions of functions that are closed under restrictions and admit L_{1,k} bounds. Finally, we apply the above structural results to obtain new unconditional pseudorandom generators and new correlation bounds for various classes of m F?-polynomials

Effect of an Injury Awareness Education Program on Risk-Taking Behaviors and Injuries in Juvenile Justice Offenders: A Retrospective Cohort Study

Background Risk-taking behavior is a leading cause of injury and death amongst young people. Methodology and Principal Findings This was a retrospective cohort study on the effectiveness of a 1-day youth injury awareness education program (Prevent Alcohol and Risk-related Trauma in Youth, P.A.R.T.Y.) program in reducing risk taking behaviors and injuries of juvenille justice offenders in Western Australia. Of the 3659 juvenile justice offenders convicted by the court magistrates between 2006 and 2010, 225 were referred to the P.A.R.T.Y. education program. In a before and after survey of these 225 participants, a significant proportion of them stated that they were more receptive to modifying their risk-taking behavior (21% before vs. 57% after). Using data from the Western Australia Police and Department of Health, the incidence of subsequent offences and injuries of all juvenile justice offenders was assessed. The incidence of subsequent traffic or violence-related offences was significantly lower for those who had attended the program compared to those who did not (3.6% vs. 26.8%; absolute risk reduction [ARR] = 23.2%, 95% confidence interval [CI] 19.9%–25.8%; number needed to benefit = 4.3, 95%CI 3.9–5.1; p = 0.001), as were injuries leading to hospitalization (0% vs. 1.6% including 0.2% fatality; ARR = 1.6%, 95%CI 1.2%–2.1%) and alcohol or drug-related offences (0% vs. 2.4%; ARR 2.4%, 95%CI 1.9%–2.9%). In the multivariate analysis, only P.A.R.T.Y. education program attendance (odds ratio [OR] 0.10, 95%CI 0.05–0.21) and a higher socioeconomic background (OR 0.97 per decile increment in Index of Relative Socioeconomic Advantage and Disadvantage, 95%CI 0.93–0.99) were associated with a lower risk of subsequent traffic or violence-related offences. Significance Participation in an injury education program involving real-life trauma scenarios was associated with a reduced subsequent risk of committing violence- or traffic-related offences, injuries, and death for juvenille justice offenders

Soft excess in the quiescent Be/X-ray pulsar RX J0812.4-3114

We report a 72 ks XMM-Newton observation of the Be/X-ray pulsar (BeXRP) RX J0812.4-3114 in quiescence (L-X approximate to 1.6 x 10(33) erg s(-1)). Intriguingly, we find a two-component spectrum, with a hard power-law (Gamma approximate to 1.5) and a soft blackbody-like excess below keV. The blackbody component is consistent in kT with a prior quiescent Chandra observation reported by Tsygankov et al. and has an inferred blackbody radius of;----10 km, consistent with emission from the entire neutron star (NS) surface. There is also mild evidence for an absorption line at approximate to and/or approximate to 1.4 keV. The hard component shows pulsations at P approximate to 31.908 s (pulsed fraction 0.84 +/- 0.10), agreeing with the pulse period seen previously in outbursts, but 110 pulsations were found in the soft excess (pulsed fraction less than or similar to 31 per cent). We conclude that the pulsed hard component suggests low-level accretion on to the NS poles, while the soft excess seems to originate from the entire NS surface. Wc speculate that, in quiescence, the source switches between a soft, thermal-dominated state (when the propeller effect is at work) and a relatively hard state with low-level accretion, and use the propeller cut-off to estimate the ma.gnetic field of the system to be less than or similar to 8.4 x 10(11)G. We compare the quiescent thermal L-X predicted by the standard deep crustal heating model to our observations and find that RX J0812.4-31.14 has a high thermal L-X, at or above the prediction for minimum cooling mechanisms. This suggests that RX J0812.4-3114 either contains a relatively low-mass NS with minimum cooling, or that the system may be young enough that the NS has not fully cooled from the supernova explosion

Measurement of Scintillation and Ionization Yield and Scintillation Pulse Shape from Nuclear Recoils in Liquid Argon

We have measured the scintillation and ionization yield of recoiling nuclei in liquid argon as a function of applied electric field by exposing a dual-phase liquid argon time projection chamber (LAr-TPC) to a low energy pulsed narrow band neutron beam produced at the Notre Dame Institute for Structure and Nuclear Astrophysics. Liquid scintillation counters were arranged to detect and identify neutrons scattered in the TPC and to select the energy of the recoiling nuclei. We report measurements of the scintillation yields for nuclear recoils with energies from 10.3 to 57.3 keV and for median applied electric fields from 0 to 970 V/cm. For the ionization yields, we report measurements from 16.9 to 57.3 keV and for electric fields from 96.4 to 486 V/cm. We also report the observation of an anticorrelation between scintillation and ionization from nuclear recoils, which is similar to the anticorrelation between scintillation and ionization from electron recoils. Assuming that the energy loss partitions into excitons and ion pairs from $^{83m}$Kr internal conversion electrons is comparable to that from $^{207}$Bi conversion electrons, we obtained the numbers of excitons ($N_{ex}$) and ion pairs ($N_i$) and their ratio ($N_{ex}/N_i$) produced by nuclear recoils from 16.9 to 57.3 keV. Motivated by arguments suggesting direction sensitivity in LAr-TPC signals due to columnar recombination, a comparison of the light and charge yield of recoils parallel and perpendicular to the applied electric field is presented for the first time.Comment: v2 to reflect published versio

Definition of the σW regulon of Bacillus subtilis in the absence of stress

Bacteria employ extracytoplasmic function (ECF) sigma factors for their responses to environmental stresses. Despite intensive research, the molecular dissection of ECF sigma factor regulons has remained a major challenge due to overlaps in the ECF sigma factor-regulated genes and the stimuli that activate the different ECF sigma factors. Here we have employed tiling arrays to single out the ECF σW regulon of the Gram-positive bacterium Bacillus subtilis from the overlapping ECF σX, σY, and σM regulons. For this purpose, we profiled the transcriptome of a B. subtilis sigW mutant under non-stress conditions to select candidate genes that are strictly σW-regulated. Under these conditions, σW exhibits a basal level of activity. Subsequently, we verified the σW-dependency of candidate genes by comparing their transcript profiles to transcriptome data obtained with the parental B. subtilis strain 168 grown under 104 different conditions, including relevant stress conditions, such as salt shock. In addition, we investigated the transcriptomes of rasP or prsW mutant strains that lack the proteases involved in the degradation of the σW anti-sigma factor RsiW and subsequent activation of the σW-regulon. Taken together, our studies identify 89 genes as being strictly σW-regulated, including several genes for non-coding RNAs. The effects of rasP or prsW mutations on the expression of σW-dependent genes were relatively mild, which implies that σW-dependent transcription under non-stress conditions is not strictly related to RasP and PrsW. Lastly, we show that the pleiotropic phenotype of rasP mutant cells, which have defects in competence development, protein secretion and membrane protein production, is not mirrored in the transcript profile of these cells. This implies that RasP is not only important for transcriptional regulation via σW, but that this membrane protease also exerts other important post-transcriptional regulatory functions

Genotyping Performance Assessment of Whole Genome Amplified DNA with Respect to Multiplexing Level of Assay and Its Period of Storage

Whole genome amplification can faithfully amplify genomic DNA (gDNA) with minimal bias and substantial genome coverage. Whole genome amplified DNA (wgaDNA) has been tested to be workable for high-throughput genotyping arrays. However, issues about whether wgaDNA would decrease genotyping performance at increasing multiplexing levels and whether the storage period of wgaDNA would reduce genotyping performance have not been examined. Using the Sequenom MassARRAY iPLEX Gold assays, we investigated 174 single nucleotide polymorphisms for 3 groups of matched samples: group 1 of 20 gDNA samples, group 2 of 20 freshly prepared wgaDNA samples, and group 3 of 20 stored wgaDNA samples that had been kept frozen at −70°C for 18 months. MassARRAY is a medium-throughput genotyping platform with reaction chemistry different from those of high-throughput genotyping arrays. The results showed that genotyping performance (efficiency and accuracy) of freshly prepared wgaDNA was similar to that of gDNA at various multiplexing levels (17-plex, 21-plex, 28-plex and 36-plex) of the MassARRAY assays. However, compared with gDNA or freshly prepared wgaDNA, stored wgaDNA was found to give diminished genotyping performance (efficiency and accuracy) due to potentially inferior quality. Consequently, no matter whether gDNA or wgaDNA was used, better genotyping efficiency would tend to have better genotyping accuracy