Resumos...

A Chefia desta unidade tem a satisfação de apresentar os resumos da VII Mostra de Trabalhos de Bolsistas e Estagiários da Embrapa Informática Agropecuária, realizada de 5 a 7 de dezembro de 2011. A Mostra de Estagiários da Embrapa Informática Agropecuária tem como objetivo complementar o desenvolvimento profissional dos estagiários e bolsistas que participam do Programa de Estágio de Complementação Educacional da Embrapa, junto às equipes de pesquisa e apoio da Unidade e Instituições parceiras, Centro de Pesquisas Meteorológicas e Climáticas Aplicadas à Agricultura (Cepagri/Unicamp), Rede Nacional de Pesquisa - RNP e Escritório de Negócios de Campinas da Embrapa SNT. Além de promover a experiência de preparação de um pôster utilizando-se de metodologia científica e da apresentação oral de seu conteúdo, o evento também contribui para uma maior integração entre o estudante e seu supervisor nas atividades conjuntas de preparação para a exposição. Acreditamos ainda que ações como essas contribuam para o sucesso profissional desses estudantes. A Embrapa Informática Agropecuária parabeniza e agradece a dedicação de todos os estudantes e seus orientadores, do Comitê Local de Publicações bem como o apoio da área administrativa, especialmente o Setor de Gestão de Pessoas, da área de Comunicação e Negócios e de todos àqueles que contribuíram para que a VII Mostra de Estagiários e Bolsistas se tornasse realidade.Editores técnicos: Marcos Dias Soares, Maria Giulia Croce, Nanci Cassini Manzoti Andrade, Silvia Maria Fonseca Silveira Massruhá

Where Morphological and Molecular Classifications Meet: The Role of p53 Immunohistochemistry in the Prognosis of Low-Risk Endometrial Carcinoma (GLAMOUR Study)

Simple Summary There is a lack of literature on the role of molecular classification in patients with morphological low-risk EC. We aimed to evaluate the incidence and prognostic role of p53 mutations in this specific subgroup of patients. Our findings show that 4.9% of low-risk EC are p53abn; the OR for the recurrence of p53abn versus p53wt patients was 5.23-CI 95% 0.98-27.95, p = 0.053. No difference in OS was observed between the two groups. Recurrences were mostly local and occur two years after diagnosis. Our data might serve as a valuable tool for clinicians' everyday practice, but larger prospective studies are urgently needed.Abstract No prospective study has validated molecular classification to guide adjuvant treatment in endometrial cancer (EC), and not even retrospective data are present for patients with morphological low-risk EC. We conducted a retrospective, multicenter, observational study including 370 patients with low-risk endometrioid EC to evaluate the incidence and prognostic role of p53 abnormal expression (p53abn) in this specific subgroup. Among 370 patients, 18 had abnormal expressions of p53 (4.9%). In 13 out of 370 patients (3.6%), recurrences were observed and two were p53abn. When adjusting for median follow-up time, the odds ratio (OR) for recurrence among those with p53abn versus p53 wild type (p53wt) was 5.23-CI 95% 0.98-27.95, p = 0.053. The most common site of recurrence was the vaginal cuff (46.2%). One recurrence occurred within the first year of follow-up, and the patient exhibited p53abn. Both 1-year and 2-year DFS rates were 94.4% and 100% in the p53abn and p53wt groups, respectively. One patient died from the disease and comprised p53wt. No difference in OS was registered between the two groups; the median OS was 21.9 months (16.4-30.1). Larger multicenter studies are needed to tailor the treatment of low-risk EC patients with p53abn. Performing molecular classification on all EC patients might be cost-effective, and despite the limits of our relatively small sample, p53abn patients seem to be at greater risk of recurrence, especially locally and after two years since diagnosis

Association of osteocalcin, osteoprotegerin, and osteopontin with cardiovascular disease and retinopathy in type 2 diabetes

Background: Novel biomarkers of vascular disease in diabetes could help identify new mechanistic pathways. Osteocalcin, osteoprotegerin, and osteopontin are key molecules involved in bone and vascular calcification processes, both of which are compromised in diabetes. We aimed to evaluate possible associations of osteocalcin, osteoprotegerin, and osteopontin with cardiovascular disease (CVD) and diabetic retinopathy (DR) among people with type 2 diabetes (T2D). Materials and methods: Osteocalcin, osteoprotegerin, and osteopontin concentrations were measured at enrolment in 848 participants with T2D from the Sapienza University Mortality and Morbidity Event Rate (SUMMER) Study (ClinicalTrials.gov: NCT02311244). Logistic regression models and propensity score matching were used to assess possible associations of osteocalcin, osteoprotegerin, and osteopontin with a history of CVD and with evidence of any grade of DR adjusting for confounders. Results: Previous CVD was reported in 139 (16.4%) participants, while 144 (17.0%) had DR. After adjusting for possible confounders, osteocalcin but not osteoprotegerin or osteopontin concentrations were associated with a history of CVD (Odds Ratio [OR] and 95% CI for one standard deviation (SD) increase in osteocalcin concentrations (natural log): 1.35 (1.06-1.72), p = 0.014). Associations with prevalent DR were seen for osteoprotegerin (OR for one SD increase in osteoprotegerin concentrations (natural log): 1.25 (1.01-1.55), p = 0.047) and osteopontin (OR for one SD increase in osteopontin concentrations (natural log): 1.25 (1.02-1.53), p = 0.022), but not osteocalcin. Conclusions: In T2D, higher serum osteocalcin concentrations are associated with macrovascular complications and higher osteoprotegerin and osteopontin concentrations with microvascular complications, suggesting that these osteokines might be involved in pathways directly related to vascular disease

Programa e resumos...

Desenvolvimento e conquistas da producao integrada de frutas no Brasil; Integracao do agronegocio para exportacao: condominios rurais e consorcios; Pif no mercado interno: o desafio no atacado e varejo; A producao integrada a maca no Brasil; A adocao dos principios da PIM no Brasil; Quatro anos de producao integrada de citros no Estado da Bahia; Fruticultura sustentavel: o caso da producao integrada - Pi-manga e Pi-uva em areas de pequeno produtor do Sub-médio São Francisco - Brasil; Avaliacao da conformidade na Producao Integrada; Marco legal de la produccion integrada de frutas en Espana; Estrutura legal da producao de frutas na Italia; Uso correto de agrotoxicos; Logistica de pos-colheita na producao integrada no Brasil; Tecnologia de pos-colheita na producao integrada de melao e caju.bitstream/CNPAT-2010/9126/1/Dc-099.pdfVII SBPIF. Editores: Jorge Anderson Guimarães, Saul Dussán Sarria, Vitor Hugo de Oliveira

Practice patterns and 90-day treatment-related morbidity in early-stage cervical cancer

To evaluate the impact of the Laparoscopic Approach to Cervical Cancer (LACC) Trial on patterns of care and surgery-related morbidity in early-stage cervical cancer

Mortality and pulmonary complications in patients undergoing surgery with perioperative SARS-CoV-2 infection: an international cohort study

Background: The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on postoperative recovery needs to be understood to inform clinical decision making during and after the COVID-19 pandemic. This study reports 30-day mortality and pulmonary complication rates in patients with perioperative SARS-CoV-2 infection. Methods: This international, multicentre, cohort study at 235 hospitals in 24 countries included all patients undergoing surgery who had SARS-CoV-2 infection confirmed within 7 days before or 30 days after surgery. The primary outcome measure was 30-day postoperative mortality and was assessed in all enrolled patients. The main secondary outcome measure was pulmonary complications, defined as pneumonia, acute respiratory distress syndrome, or unexpected postoperative ventilation. Findings: This analysis includes 1128 patients who had surgery between Jan 1 and March 31, 2020, of whom 835 (74·0%) had emergency surgery and 280 (24·8%) had elective surgery. SARS-CoV-2 infection was confirmed preoperatively in 294 (26·1%) patients. 30-day mortality was 23·8% (268 of 1128). Pulmonary complications occurred in 577 (51·2%) of 1128 patients; 30-day mortality in these patients was 38·0% (219 of 577), accounting for 81·7% (219 of 268) of all deaths. In adjusted analyses, 30-day mortality was associated with male sex (odds ratio 1·75 [95% CI 1·28–2·40], p\textless0·0001), age 70 years or older versus younger than 70 years (2·30 [1·65–3·22], p\textless0·0001), American Society of Anesthesiologists grades 3–5 versus grades 1–2 (2·35 [1·57–3·53], p\textless0·0001), malignant versus benign or obstetric diagnosis (1·55 [1·01–2·39], p=0·046), emergency versus elective surgery (1·67 [1·06–2·63], p=0·026), and major versus minor surgery (1·52 [1·01–2·31], p=0·047). Interpretation: Postoperative pulmonary complications occur in half of patients with perioperative SARS-CoV-2 infection and are associated with high mortality. Thresholds for surgery during the COVID-19 pandemic should be higher than during normal practice, particularly in men aged 70 years and older. Consideration should be given for postponing non-urgent procedures and promoting non-operative treatment to delay or avoid the need for surgery. Funding: National Institute for Health Research (NIHR), Association of Coloproctology of Great Britain and Ireland, Bowel and Cancer Research, Bowel Disease Research Foundation, Association of Upper Gastrointestinal Surgeons, British Association of Surgical Oncology, British Gynaecological Cancer Society, European Society of Coloproctology, NIHR Academy, Sarcoma UK, Vascular Society for Great Britain and Ireland, and Yorkshire Cancer Research

The Dark Side of the Web-A Risk for Children and Adolescents Challenged by Isolation during the Novel Coronavirus 2019 Pandemic

In response to the global novel coronavirus disease 2019 (COVID-19) pandemic, many countries around the world adopted social isolation measures to contain the spread of the virus.1 For children and adolescents, limitations in faceto-face activities and interactions with their traditional peer groups has been a frustrating experience. After disease containment measures, which included school closures, social distancing, and home quarantine, children and adolescents faced a prolonged state of physical isolation from their peers, teachers, extended family, and community networks that affects their emotional and behavioral health.2 Parents and pediatricians are reporting signs of mental distress in children of all ages within the context of the pandemic.3 In several cases, this unexpected social isolation has paradoxically improved the psychosocial state of fearful children, and the mental health of those who have been victims of bullying.4 School function improved with distance learning and socialization may have increased using virtual connections to create a larger social group.5 However, children and adolescents who experience a prolonged state of physical isolation may look for alternative, somehow attractive or unconventional forms of socialization, available on the internet. Children may be exposed to the risks of unsupervised cyberspace exploration beyond the open web, which may lead them to areas that are usually not available to visitors. They may pass the gates of the “open” and “deep web” sections and enter into the dangerous “dark web” zones, which predominantly host unethical and criminal activities. In those shadowy corners of the worldwide web, there exist dangers ranging from identity theft and the drug trade to suicide chat rooms and child pornography.6 This commentary, authored by European Paediatric Association/Union of National European Paediatric Societies and Associations members of the working group on social pediatrics, briefly discusses the features of the dark web and its implications for children and adolescents. Our aim is to raise awareness of pediatricians and families on the growing risk of child exploitation through the web at a time when vulnerable young people face home lockdowns with potential abusers intruding on their privac

Long-term prognosis of juvenile myoclonic epilepsy: A systematic review searching for sex differences

Purpose: Juvenile myoclonic epilepsy (JME), like other forms of idiopathic generalized epilepsy, shows a marked female predominance. However, few studies have specifically addressed the role of sex in its long-term prognosis. We performed a systematic review of the literature relevant to JME prognosis, focusing on sex-based differences in prognostic factors and outcome. Methods: A comprehensive literature search of the PubMed and Scopus databases was performed, considering all articles up to April 2020 in which long-term prognosis in JME had been explored and sex differences in outcome or prognostic factors were specified. Results: We included 25 articles published between 1984 and 2020. Sex differences in epilepsy outcome were explored by 21 of the 25 studies, but only three reported different outcomes in male vs female patients. All three found female sex to be associated with a later response to antiseizure medications, worse seizure control, and a higher risk of relapse in their entire study samples, which included JME patients. Eight studies found sex-based differences in possible predictors of long-term outcome: prolonged epileptiform EEG runs and the presence of eye closure sensitivity, both more frequent in women, were factors possibly linked to a poorer prognosis, as were praxis induction and generalized EEG asymmetric changes, which instead were more common in men. Valproate use, more frequent in men, was associated with a better outcome. Conclusion: Most studies do not highlight sex differences in JME prognosis. However, some sex specificities do emerge, especially with regard to particular reflex traits and EEG abnormalities. Finally, sex may condition therapeutic choices, and thus have a possible impact on long-term outcome