Digital transformation in the footwear industry : assessing the potential of IoT on portuguese SMEs in the footwear industry

In the last few years, manufacturing companies, from the most diverse industries and sectors, have been investing in industry 4.0 technologies. One of these technologies is the Internet of Things (IoT), which has been revolutionising industries, namely manufacturing, healthcare, energy, retail, and agriculture. Manufacturing firms are adopting IoT not only to improve quality, production and reduce costs but also to enhance the logistic chain and improve customer experience. However, very little progress has been made in the Portuguese footwear industry towards the implementation of the Internet of Things, particularly in the SME segment. This dissertation has analysed the potential operational and production advantages of IoT, the associated economic impact, as well as the limitations of this technology and the challenges for its implementation in Portuguese footwear SMEs. Finally, this dissertation aims to present an adoption roadmap. The results provided positive indicators of operational and productivity gains translated into cost reduction in work insurance, energy, travelling, acquisition of raw materials and decrease of inventory units, and production waste. Nevertheless, the associated potential of each IoT use case is dependent on the capacity of Portuguese footwear SMEs to invest and acquire the necessary technical resources. The main barriers to the adoption of IoT derive from the limitations of the technology and the specific challenges associated to the Portuguese footwear SMEs economic and technical framework. Nonetheless, the technological advances, the cross collaboration among the different footwear stakeholders, and a well-defined implementation strategy can overcome these barriers and bring operational and economic benefits.A Internet of Things (IoT), uma tecnologia da indústria 4.0, tem revolucionado indústrias como a da manufactura, saúde, energia, retalho e agricultura. As empresas de manufactura estão a adoptar a IoT não apenas para melhorar a qualidade dos productos, eficiência produtiva, e reduzir custos, mas também para aprimorar a cadeia logística e melhorar a experiência do cliente. Contudo, poucos foram os progressos feitos referentes à IoT na indústria do calçado Português, principalmente no segmento das PMEs. A seguinte dissertação analisou as potenciais vantagens operacionais e productivas da IoT e os seus impactos económicos, bem como as limitações da tecnologia e os desafios de implementação para as PME do calçado Português. Por último, é apresentado um plano de implementação da tecnologia. Subsequentemente os resultados mostraram indicadores favoráveis de ganhos operacionais e produtivos, que se traduzem na redução de custos com seguros de trabalho, inventário, desperdícios de produção, custos de manutenção, encargos com deslocações, custos de energia e custos de aquisição de matérias-primas. Contudo, o potencial de cada caso de uso de IoT depende da capacidade das PMEs do calçado Português para fazer o investimento financeiro e adquirir os recursos técnicos necessários. As principais barreiras à adopção da IoT derivam das limitações da própria tecnologia e dos desafios relativos ao quadro económico e técnico das PMEs Portuguesas do calçado. Não obstante, os avanços tecnológicos, a colaboração entre os diferentes intervenientes e uma estratégia de implementação bem definida podem superar estas barreiras e garantir a adopção e trazer ganhos operacionais e económicos

Is gut microbiota the key?

Funding: This study was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT—Fundação para a Ciência e a Tecnologia within CINTESIS, R&D Unit (reference UID/IC/4255/2013) and CHRC (UIDB/04923/2020 and UIDP/04923/2020). This study was also supported by Emilio Peres grant from the Portuguese Society of Diabetology.The Mediterranean diet (MD) has been recommended for type 2 diabetes (T2D) treatment. The impact of diet in shaping the gut microbiota is well known, particularly for MD. However, the link between MD and diabetes outcome improvement is not completely clear. This study aims to evaluate the role of microbiota modulation by a nonpharmacological intervention in patients with T2D. In this 12-week single-arm pilot study, nine participants received individual nutritional counseling sessions promoting MD. Gut microbiota, biochemical parameters, body composition, and blood pressure were assessed at baseline, 4 weeks, and 12 weeks after the intervention. Adherence to MD [assessed by Mediterranean Diet Adherence Screener (MEDAS) score] increased after the intervention. Bacterial richness increased after 4 weeks of intervention and was negatively correlated with fasting glucose levels and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR). Prevotella to Bacteroides ratio also increased after 4 weeks. In contrast, glycated haemoglobin (HbA1c) and HOMA-IR were only decreased at the end of study. Alkaline phosphatase activity was assessed in fecal samples and was negatively correlated with HbA1c and positively correlated with bacterial diversity. The results of this study reinforce that MD adherence results in a better glycemic control in subjects with T2D. Changes in gut bacterial richness caused by MD adherence may be relevant in mediating the metabolic impact of this dietary intervention.publishersversionpublishe

Streptococcus pyogenes Causing Skin and Soft Tissue Infections Are Enriched in the Recently Emerged emm89 Clade 3 and Are Not Associated With Abrogation of CovRS

Although skin and soft tissue infections (SSTI) are the most common focal infections associated with invasive disease caused by Streptococcus pyogenes (Lancefield Group A streptococci - GAS), there is scarce information on the characteristics of isolates recovered from SSTI in temperate-climate regions. In this study, 320 GAS isolated from SSTI in Portugal were characterized by multiple typing methods and tested for antimicrobial susceptibility and SpeB activity. The covRS and ropB genes of isolates with no detectable SpeB activity were sequenced. The antimicrobial susceptibility profile was similar to that of previously characterized isolates from invasive infections (iGAS), presenting a decreasing trend in macrolide resistance. However, the clonal composition of SSTI between 2005 and 2009 was significantly different from that of contemporary iGAS. Overall, iGAS were associated with emm1 and emm3, while SSTI were associated with emm89, the dominant emm type among SSTI (19%). Within emm89, SSTI were only significantly associated with isolates lacking the hasABC locus, suggesting that the recently emerged emm89 clade 3 may have an increased potential to cause SSTI. Reflecting these associations between emm type and disease presentation, there were also differences in the distribution of emm clusters, sequence types, and superantigen gene profiles between SSTI and iGAS. According to the predicted ability of each emm cluster to interact with host proteins, iGAS were associated with the ability to bind fibrinogen and albumin, whereas SSTI isolates were associated with the ability to bind C4BP, IgA, and IgG. SpeB activity was absent in 79 isolates (25%), in line with the proportion previously observed among iGAS. Null covS and ropB alleles (predicted to eliminate protein function) were detected in 10 (3%) and 12 (4%) isolates, corresponding to an underrepresentation of mutations impairing CovRS function in SSTI relative to iGAS. Overall, these results indicate that the isolates responsible for SSTI are genetically distinct from those recovered from normally sterile sites, supporting a role for mutations impairing CovRS activity specifically in invasive infection and suggesting that this role relies on a differential regulation of other virulence factors besides SpeB

Streptococcus canis Are a Single Population Infecting Multiple Animal Hosts Despite the Diversity of the Universally Present M-Like Protein SCM

Streptococcus canis is an animal pathogen which occasionally causes infections in humans. The S. canis M-like protein (SCM) encoded by the scm gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of the scm gene in 188 S. canis isolates recovered from companion animals (n = 152), wild animal species (n = 20), and humans (n = 14). Multilocus sequence typing, including the first characterization of wildlife isolates, showed that the same lineages are present in all animal hosts, raising the possibility of extensive circulation between species. Whole-genome analysis revealed that emm-like genes found previously in S. canis correspond to divergent scm genes, indicating that what was previously believed to correspond to two genes is in fact the same scm locus. We designed primers allowing for the first time the successful amplification of the scm gene in all isolates. Analysis of the scm sequences identified 12 distinct types, which could be divided into two clusters: group I (76%, n = 142) and group II (24%, n = 46) sharing little sequence similarity. The predicted group I SCM showed extensive similarity with each other outside of the N-terminal hypervariable region and a conserved IgG binding domain. This domain was absent from group II SCM variants found in isolates previously thought to lack the scm gene, which also showed greater amino acid variability. Further studies are necessary to elucidate the possible host interacting partners of the group II SCM variants and their role in virulence

Estudo Storytelling: Pela voz e criatividade de profissionais das áreas da saúde

Contar histórias, ou partilhar uma perspetiva pessoal, é uma forma essencial de comunicação e um veículo de ligação humana. Os seres humanos são intrinsecamente narradores de histórias. As histórias são uma das formas fundamentais de dar sentido ao mundo, aprender e compreender. A leitura de narrativas - storytelling - permite, nas áreas da saúde, um efeito muito positivo. O storytelling tem provado que, através da narrativa e da associação a elementos que fortalecem a imaginação, os seus destinatários compreendem melhor a mensagem porque esta fica associada à criatividade, imaginação e a um certo humor, levando as pessoas a agirem mais facilmente. Partindo do princípio que se pretende que as pessoas compreendam melhor como gerir a sua saúde em todo o seu ciclo de vida, o storytelling consegue captar a atenção, de uma forma simples e acessível, trabalhando as memórias, a intenção de comportamento, o envolvimento e, por fim, a ação conducente a uma melhor saúde. Neste sentido, um grupo ativo de vinte profissionais das áreas da saúde juntou-se para oferecer ao público estas narrativas escritas e orais (podcast) com conteúdos sobre saúde: vacinação, medicação, exercício físico e longevidade. O objetivo deste projeto foi tratar de assuntos de saúde através da narração de histórias do dia-a-dia que podem ser contadas e passadas em vários ambientes de saúde, desde hospitais, clínicas, unidades de saúde e outros espaços. Neste âmbito, através de uma metodologia qualitativa de cocriação, foi criado um podcast que reúne, pela primeira vez, um projeto de storytelling feito por profissionais das várias áreas da saúde no âmbito da área da literacia em saúde na prática, constituído por sete histórias narradas, de acordo com os princípios do storytelling, com o intuito de contribuir para melhores resultados em saúde através da melhoria do bem-estar de quem as ouve. As narrações sobre saúde, contadas de modo a estimular a fantasia e a agradabilidade da audição, poderão ser uma ferramenta promotora de um melhor acesso, compreensão e uso da informação em saúde, que conduz a melhores tomadas de decisão e, por isso, melhores resultados em saúde. O que tem de inovação é a capacidade transformadora que os profissionais das áreas da saúde têm para desenvolver, para além das competências técnicas, as suas competências relacionais e criativas que visam melhorar a saúde das pessoas e tornar este mundo complexo da saúde mais acessível. As implicações práticas deste projeto visam estimular e incentivar outros profissionais das áreas da saúde, sociais e da educação a contribuírem com o seu talento, criatividade e imaginação a criarem narrativas que, juntando a emoção e a razão, permitam uma melhor literacia em saúde do cidadão.info:eu-repo/semantics/publishedVersio

Relatório vacinação estratégias de comunicação e literacia em saúde eficazes para população jovem

Um conjunto de duas dezenas de profissionais portugueses de várias áreas da saúde refletiu em conjunto sobre as questões que levam os jovens a não se interessarem pela vacinação contra o COVID-19 e a forma mais eficaz de se ultrapassarem estas crenças negativas e barreiras à vacinação. As preocupações destes jovens sobre os efeitos seguros da vacinação, a proteção de familiares e amigos e o regresso às atividades sociais parecem ser fatores motivadores para a ação de vacinação e proteção. Neste sentido, e reunindo o conhecimento sobre as campanhas de marketing em saúde e os conteúdos comunicativos que podem influenciar positivamente esta mobilização para a vacinação, estes profissionais apresentam neste relatório um conjunto de dados científicos, estratégicos e operacionais que podem apontar alguns caminhos. Este grupo de trabalho avaliou o perfil dos jovens (dados demográficos e psicográficos), as crenças, as necessidades, a perceção do risco e as motivações que podem ser atendidas para que se criem, com a brevidade possível, através dos recursos públicos e das autoridades sanitárias, onde se inclui a Direção-Geral da Saúde (DGS), formas de melhor promover os objetivos inequívocos: vacinar a população jovem em Portugal com a brevidade possível.info:eu-repo/semantics/publishedVersio

An Integrative View of the Phyllosphere Mycobiome of Native Rubber Trees in the Brazilian Amazon

The rubber tree, Hevea brasiliensis, is a neotropical Amazonian species. Despite its high economic value and fungi associated with native individuals, in its original area in Brazil, it has been scarcely investigated and only using culture-dependent methods. Herein, we integrated in silico approaches with novel field/experimental approaches and a case study of shotgun metagenomics and small RNA metatranscriptomics of an adult individual. Scientific literature, host fungus, and DNA databases are biased to fungal taxa, and are mainly related to rubber tree diseases and in non-native ecosystems. Metabarcoding retrieved specific phyllospheric core fungal communities of all individuals, adults, plantlets, and leaves of the same plant, unravelling hierarchical structured core mycobiomes. Basidiomycotan yeast-like fungi that display the potential to produce antifungal compounds and a complex of non-invasive ectophytic parasites (Sooty Blotch and Flyspeck fungi) co-occurred in all samples, encompassing the strictest core mycobiome. The case study of the same adult tree (previously studied using culture-dependent approach) analyzed by amplicon, shotgun metagenomics, and small RNA transcriptomics revealed a high relative abundance of insect parasite-pathogens, anaerobic fungi and a high expression of Trichoderma (a fungal genus long reported as dominant in healthy wild rubber trees), respectively. Altogether, our study unravels new and intriguing information/hypotheses of the foliar mycobiome of native H. brasiliensis, which may also occur in other native Amazonian trees

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved