New insights on Neolithic food and mobility patterns of Mediterranean coastal populations

The aims of this research are to explore the diet, mobility, social organization, and environmental exploitation patterns of early Mediterranean farmers, particularly the role of marine and plant resources in these foodways. In addition, this work strives to document possible gendered patterns of behavior linked to the neolithization of this ecologically rich area. To achieve this, a set of multiproxy analyses (isotopic analyses, dental calculus, microremains analysis, ancient DNA) were performed on an exceptional deposit (n = 61) of human remains from the Les Bréguières site (France), dating to the transition of the sixth to the fifth millennium BCE. Materials and Methods: The samples used in this study were excavated from the Les Bréguières site (Mougins, Alpes-Maritimes, France), located along the southeastern Mediterranean coastline of France. Stable isotope analyses (C, N) on bone collagen (17 coxal bones, 35 craniofacial elements) were performed as a means to infer protein intake during tissue development. Sulfur isotope ratios were used as indicators of geographical and environmental points of origin. The study of ancient dental calculus helped document the consumption of plants. Strontium isotope analysis on tooth enamel (n = 56) was conducted to infer human provenance and territorial mobility. Finally, ancient DNA analysis was performed to study maternal versus paternal diversity within this Neolithic group (n = 30). Results: Stable isotope ratios for human bones range from −20.3 to −18.1¿ for C, from 8.9 to 11.1¿ for N and from 6.4 to 15¿ for S. Domestic animal data range from −22.0 to −20.2¿ for C, from 4.1 to 6.9¿ for N, and from 10.2 to 12.5¿ for S. Human enamel 87Sr/86Sr range from 0.7081 to 0.7102, slightly wider than the animal range (between 0.7087 and 0.7096). Starch and phytolith microremains were recovered as well as other types of remains (e.g., hairs, diatoms, fungal spores). Starch grains include Triticeae type and phytolith includes dicotyledons and monocot types as panicoid grasses. Mitochondrial DNA characterized eight different maternal lineages: H1, H3, HV (5.26%), J (10.53%), J1, K, T (5.2%), and U5 (10.53%) but no sample yielded reproducible Y chromosome SNPs, preventing paternal lineage characterization. Discussion: Carbon and nitrogen stable isotope ratios indicate a consumption of protein by humans mainly focused on terrestrial animals and possible exploitation of marine resources for one male and one undetermined adult. Sulfur stable isotope ratios allowed distinguishing groups with different geographical origins, including two females possibly more exposed to the sea spray effect. While strontium isotope data do not indicate different origins for the individuals, mitochondrial lineage diversity from petrous bone DNA suggests the burial includes genetically differentiated groups or a group practicing patrilocality. Moreover, the diversity of plant microremains recorded in dental calculus provide the first evidence that the groups of Les Bréguières consumed a wide breadth of plant foods (as cereals and wild taxa) that required access to diverse environments. This transdisciplinary research paves the way for new perspectives and highlights the relevance for novel research of contexts (whether recently discovered or in museum collections) excavated near shorelines, due to the richness of the biodiversity and the wide range of edible resources available

Investigating mitochondrial DNA relationships in Neolithic Western Europe through serial coalescent simulations

Recent ancient DNA studies on European Neolithic human populations have provided persuasive evidence of a major migration of farmers originating from the Aegean, accompanied by sporadic hunter-gatherer admixture into early Neolithic populations, but increasing toward the Late Neolithic. In this context, ancient mitochondrial DNA data collected from the Neolithic necropolis of Gurgy (Paris Basin, France), the largest mitochondrial DNA sample obtained from a single archeological site for the Early/Middle Neolithic period, indicate little differentiation from farmers associated to both the Danubian and Mediterranean Neolithic migration routes, as well as from Western European hunter-gatherers. To test whether this pattern of differentiation could arise in a single unstructured population by genetic drift alone, we used serial coalescent simulations. We explore female effective population size parameter combinations at the time of the colonization of Europe 45000 years ago and the most recent of the Neolithic samples analyzed in this study 5900 years ago, and identify conditions under which population panmixia between hunter-gatherers/Early-Middle Neolithic farmers and Gurgy cannot be rejected. In relation to other studies on the current debate of the origins of Europeans, these results suggest increasing hunter-gatherer admixture into farmers' group migrating farther west in Europe.European Journal of Human Genetics advance online publication, 28 December 2016; doi:10.1038/ejhg.2016.180

Les individus du dépôt multiple néolithique du site de Pontcharaud, Clermont-Ferrand (fouilles 1986) : guerriers ou morts d’accompagnement ?

Le site de Pontcharaud 2 (4300-3900 cal. BCE), fouillé en 1986, a livré 68 individus issus de 56 structures parmi lesquelles un dépôt multiple exceptionnel de par le nombre de sujets qui le compose (5 adultes et 2 immatures) et la présence d’une pointe de flèche dans la vertèbre de l’un d’entre eux. Cette découverte a été abordée dans plusieurs publications sans qu’il y ait consensus quant à l’interprétation qui peut en être donnée. Or, la série ostéologique de Pontcharaud 2 bénéficie depuis..

L’alimentation des premiers agropasteurs du Néolithique : apport de l’étude des microrestes du tartre dentaire des individus de la Roussille (Auvergne)

Le Néolithique, épisode majeur de l’histoire humaine est marqué par la domestication des espèces végétales et animales, les débuts de l’agriculture et une croissance démographique significative. Le projet ANR WomenSOFar (ANR-21-CE03-0008) vise à identifier le statut des femmes à travers la façon dont elles se déplacent, se nourrissent, travaillent et prennent soin des jeunes. Dans le cadre de ce projet de recherche, une approche novatrice et exploratoire, consistant en l’analyse des micro-re..

Étude biologique des sujets mésolithiques de l’Abri du Squelette (Les Eyzies, Dordogne, France)

L’Abri du Squelette, découvert en 1938 sur les rives de la Vézère (Dordogne, France) a livré les restes squelettiques d’au moins 3 individus qui n’ont été que récemment reconnus comme mésolithiques. La collection ostéologique est composée de vestiges recueillis lors de deux opérations archéologiques (2012, 2018) et de ceux conservés au Musée national de Préhistoire. Une étude biologique interdisciplinaire de ces restes humains a été entreprise. Les deux sujets étudiés s’inscrivent dans la va..

Cases of trisomy 21 and trisomy 18 among historic and prehistoric individuals discovered from ancient DNA

Aneuploidies, and in particular, trisomies represent the most common genetic aberrations observed in human genetics today. To explore the presence of trisomies in historic and prehistoric populations we screen nearly 10,000 ancient human individuals for the presence of three copies of any of the target autosomes. We find clear genetic evidence for six cases of trisomy 21 (Down syndrome) and one case of trisomy 18 (Edwards syndrome), and all cases are present in infant or perinatal burials. We perform comparative osteological examinations of the skeletal remains and find overlapping skeletal markers, many of which are consistent with these syndromes. Interestingly, three cases of trisomy 21, and the case of trisomy 18 were detected in two contemporaneous sites in early Iron Age Spain (800-400 BCE), potentially suggesting a higher frequency of burials of trisomy carriers in those societies. Notably, the care with which the burials were conducted, and the items found with these individuals indicate that ancient societies likely acknowledged these individuals with trisomy 18 and 21 as members of their communities, from the perspective of burial practice

Inférences sur les réseaux régionaux à partir d’arbres génétiques locaux, l’exemple du site néolithique de Gurgy 'les Noisats'

La reconstitution de la parenté génétique dans des contextes archéologiques a longtemps été impossible, mais grâce à l’optimisation récente des méthodes d’analyse de l’ADN ancien, on peut désormais reconstituer de manière fiable des généalogies génétiques, parfois importantes. L’analyse génomique approfondie du site français du Néolithique moyen de Gurgy 'les Noisats', dans le Bassin parisien, nous a permis de reconstruire deux arbres conséquents, l’un reliant 63 individus sur sept génératio..

Origin and mobility of Iron Age Gaulish groups in present-day France revealed through archaeogenomics

The Iron Age period occupies an important place in French history, as the Gauls are regularly presented as the direct ancestors of the extant French population. We documented here the genomic diversity of Iron Age communities originating from six French regions. The 49 acquired genomes permitted us to highlight an absence of discontinuity between Bronze Age and Iron Age groups in France, lending support to a cultural transition linked to progressive local economic changes rather than to a massive influx of allochthone groups. Genomic analyses revealed strong genetic homogeneity among the regional groups associated with distinct archaeological cultures. This genomic homogenisation appears to be linked to individuals’ mobility between regions as well as gene flow with neighbouring groups from England and Spain. Thus, the results globally support a common genomic legacy for the Iron Age population of modern-day France that could be linked to recurrent gene flow between culturally differentiated communities

Ten millennia of hepatitis B virus evolution

Hepatitis B virus (HBV) has been infecting humans for millennia and remains a global health problem, but its past diversity and dispersal routes are largely unknown. We generated HBV genomic data from 137 Eurasians and Native Americans dated between ~10,500 and ~400 years ago. We date the most recent common ancestor of all HBV lineages to between ~20,000 and 12,000 years ago, with the virus present in European and South American hunter-gatherers during the early Holocene. After the European Neolithic transition, Mesolithic HBV strains were replaced by a lineage likely disseminated by early farmers that prevailed throughout western Eurasia for ~4000 years, declining around the end of the 2nd millennium BCE. The only remnant of this prehistoric HBV diversity is the rare genotype G, which appears to have reemerged during the HIV pandemic

Des monuments et des hommes. La nécropole des Hauts de l’Orne à Fleury-sur-Orne (Calvados)

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