Solanum incanum extract enhances wound healing and tissue regeneration in burn mice model

This study was conducted to evaluate the topical efficacy of Solanum incanum for the treatment of partial-thickness burn in mice model. Mice were treated with topical ointment of S. incanum three times daily for 14 days. The wound healing was observed through wound contraction and histological parameters. The group treated with S. incanum ointment showed 81% reduction in wound area as compared to negative control where wound area reduced to 22%. The histological analysis further confirmed that ointment favors the tissue regeneration and reepithelization thus heal wound rapidly as com-pared to other groups. In conclusion, S. incanum extract enhances wound healing and tissue regeneration

Splenocyte microvesicles are pro-senescent endothelial effectors : impact of age and protection by EPA/DHA 6/1, an optimized formulation of nutritional eicosapentaenoic and docosahexaenoic polyunsaturated fatty acids

L’âge conduit à une senescence progressive des cellules endothéliales (ECs) favorisant une dysfonction endothéliale et vasculaire, souvent associée aux maladies cardiovasculaires. Nous avons étudié son impact sur l’émission de microvésicules (SMVs) par les leucocytes isolés de la rate de rats jeunes, d’âge moyen, ou vieux (J, MA,V) et caractérisé leurs propriétés pro-sénescentes sur des EC primaires d’artères coronaires porcines. Avec l’âge, le stress oxydant s’accumule dans la rate et l’émission de SMVs augmente. Seules les SMVs de MA et O induisent une sénescence des ECs prématurée avec une activité Senescence-Associated-β-galactosidase et un stress oxydant augmentés, la surexpression des marqueurs p53, p21, p16, un phénotype pro-inflammatoire et pro-arthérogène et une sous-expression de la eNOS. Un gavage de 7 jours avec des omega-3 EPA:DHA 6:1 mais pas EPA:DHA 1:1, réduit le stress oxydant splénique des MA et des O, et la vésiculation. EPA:DHA 6:1 abolit le caractère pro-sénescent des SMVs de MA, probablement via la réduction du stress oxydant et en prévenant l’activation du système angiotensine local.Ageing is associated with progressive endothelial senescence favoring endothelial and vascular dysfunction, often associated with cardiovascular diseases. We investigated in young, middle-aged and old rats (Y, MA, O) the impact of ageing on the shedding of spleen-derived leukocytes microvesicles (SMVs) and measured their pro-senescent effects in porcine primary coronary artery endothelial cells (ECs). Oxidative stress accumulates in spleen tissue and SMVs shedding increases with age. SMVs from MA, O but not Y rats induced premature endothelial senescence, with increased Senescence-Associated-β-galactosidase activity and up-regulated p53, p21, p16. SMVs shifted ECs towards a pro-inflammatory and pro-atherothrombotic phenotype with increased endothelial oxidative stress and down-regulated eNOS. Short-term intake of omega-3 EPA:DHA 6:1 but not EPA:DHA 1:1 reduced age-related oxidative stress and SMVs shedding in MA and O spleen tissues, and abolished SMVs-induced premature endothelial senescence in MA, most probably by reducing oxidative stress and preventing the activation of the local angiotensin system

Les microvésicules splénocytaires effecteurs de la sénescence endothéliale : impact de l'âge et protection par apport nutritionnel d'une formule optimisée d'acides gras poly-insaturés eicosapentaénoïque et docosahexaénoïque, EPA/DHA 6/1

Ageing is associated with progressive endothelial senescence favoring endothelial and vascular dysfunction, often associated with cardiovascular diseases. We investigated in young, middle-aged and old rats (Y, MA, O) the impact of ageing on the shedding of spleen-derived leukocytes microvesicles (SMVs) and measured their pro-senescent effects in porcine primary coronary artery endothelial cells (ECs). Oxidative stress accumulates in spleen tissue and SMVs shedding increases with age. SMVs from MA, O but not Y rats induced premature endothelial senescence, with increased Senescence-Associated-β-galactosidase activity and up-regulated p53, p21, p16. SMVs shifted ECs towards a pro-inflammatory and pro-atherothrombotic phenotype with increased endothelial oxidative stress and down-regulated eNOS. Short-term intake of omega-3 EPA:DHA 6:1 but not EPA:DHA 1:1 reduced age-related oxidative stress and SMVs shedding in MA and O spleen tissues, and abolished SMVs-induced premature endothelial senescence in MA, most probably by reducing oxidative stress and preventing the activation of the local angiotensin system.L’âge conduit à une senescence progressive des cellules endothéliales (ECs) favorisant une dysfonction endothéliale et vasculaire, souvent associée aux maladies cardiovasculaires. Nous avons étudié son impact sur l’émission de microvésicules (SMVs) par les leucocytes isolés de la rate de rats jeunes, d’âge moyen, ou vieux (J, MA,V) et caractérisé leurs propriétés pro-sénescentes sur des EC primaires d’artères coronaires porcines. Avec l’âge, le stress oxydant s’accumule dans la rate et l’émission de SMVs augmente. Seules les SMVs de MA et O induisent une sénescence des ECs prématurée avec une activité Senescence-Associated-β-galactosidase et un stress oxydant augmentés, la surexpression des marqueurs p53, p21, p16, un phénotype pro-inflammatoire et pro-arthérogène et une sous-expression de la eNOS. Un gavage de 7 jours avec des omega-3 EPA:DHA 6:1 mais pas EPA:DHA 1:1, réduit le stress oxydant splénique des MA et des O, et la vésiculation. EPA:DHA 6:1 abolit le caractère pro-sénescent des SMVs de MA, probablement via la réduction du stress oxydant et en prévenant l’activation du système angiotensine local

Splenocyte microvesicles are pro-senescent endothelial effectors : impact of age and protection by EPA/DHA 6/1, an optimized formulation of nutritional eicosapentaenoic and docosahexaenoic polyunsaturated fatty acids

L’âge conduit à une senescence progressive des cellules endothéliales (ECs) favorisant une dysfonction endothéliale et vasculaire, souvent associée aux maladies cardiovasculaires. Nous avons étudié son impact sur l’émission de microvésicules (SMVs) par les leucocytes isolés de la rate de rats jeunes, d’âge moyen, ou vieux (J, MA,V) et caractérisé leurs propriétés pro-sénescentes sur des EC primaires d’artères coronaires porcines. Avec l’âge, le stress oxydant s’accumule dans la rate et l’émission de SMVs augmente. Seules les SMVs de MA et O induisent une sénescence des ECs prématurée avec une activité Senescence-Associated-β-galactosidase et un stress oxydant augmentés, la surexpression des marqueurs p53, p21, p16, un phénotype pro-inflammatoire et pro-arthérogène et une sous-expression de la eNOS. Un gavage de 7 jours avec des omega-3 EPA:DHA 6:1 mais pas EPA:DHA 1:1, réduit le stress oxydant splénique des MA et des O, et la vésiculation. EPA:DHA 6:1 abolit le caractère pro-sénescent des SMVs de MA, probablement via la réduction du stress oxydant et en prévenant l’activation du système angiotensine local.Ageing is associated with progressive endothelial senescence favoring endothelial and vascular dysfunction, often associated with cardiovascular diseases. We investigated in young, middle-aged and old rats (Y, MA, O) the impact of ageing on the shedding of spleen-derived leukocytes microvesicles (SMVs) and measured their pro-senescent effects in porcine primary coronary artery endothelial cells (ECs). Oxidative stress accumulates in spleen tissue and SMVs shedding increases with age. SMVs from MA, O but not Y rats induced premature endothelial senescence, with increased Senescence-Associated-β-galactosidase activity and up-regulated p53, p21, p16. SMVs shifted ECs towards a pro-inflammatory and pro-atherothrombotic phenotype with increased endothelial oxidative stress and down-regulated eNOS. Short-term intake of omega-3 EPA:DHA 6:1 but not EPA:DHA 1:1 reduced age-related oxidative stress and SMVs shedding in MA and O spleen tissues, and abolished SMVs-induced premature endothelial senescence in MA, most probably by reducing oxidative stress and preventing the activation of the local angiotensin system

Genome wide analysis of recurrent parent genome recovery in different backcross populations for blast resistance through SSR markers in rice

Marker-assisted backcross breeding technique has been widely applied for incorporation of blast resistance genes into rice varieties. The monitoring of resistance genes along with recovery of recurrent parent is an essential aspect for reducing the donor genome content in backcross population. Current research was conducted to determine the recovery of recurrent parent in each backcross population of rice. Malaysian high yielding but blast susceptible rice variety MR219 was taken as recurrent parent while PongsuSeribu 2 was donor parent. Microsatellite markers commonly called as Simple sequence repeat markers were used to estimate recovery of recurrent parent genome i.e. MR219 in early generation of backcross population. A total of 300 microsatellite markerswere randomly applied on 12 rice chromosomes. 72 SSR markers found distinct and clear polymorphic between the parent PongsuSeribu 2 and MR219. These 72 polymorphic markers were utilized to analyze the recovery of plants in further subsequent generations. The background recovery ranged from 73-93.9% and 79- 96.3% in BC1F1 and BC2F1 generation. The proportion of recurrent parent genome increased and donor genome content decreases after every backcross generation. The recovery of recurrent parent genome content ranged from 94-97.1% in the selected improved advance lines of BC2F2 generation. In the advance lines, the average percent for the proportion of recurrent parent genome was 96.16%. Marker-assisted backcrossing efficiently accelerated the recurrent parent genome recovery within few backcrosses, reduced the backcross generation and saved plenty of time to intogress the genes against blast disease. The present results will be helpful for rice breeders for selecting true blast resistant lines along with maximum resemblance with recurrent parent

Thrombin Induces Angiotensin II-Mediated Senescence in Atrial Endothelial Cells: Impact on Pro-Remodeling Patterns

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Ageing enhances the shedding of splenocyte microvesicles with endothelial pro-senescent effect that is prevented by a short-term intake of omega-3 PUFA EPA:DHA 6:1

International audienceAgeing enhances the shedding of splenocyte microvesicles with endothelial pro-senescent effect that is prevented by a short-term intake of omega-3 PUFA EPA:DHA 6:1