Multispectral imaging flow cytometry with spatially and spectrally resolving snapshot-mosaic cameras for the characterization and classification of bioparticles

In the development and optimization of biotechnological cultivation processes the continuous monitoring through the acquisition and interpretation of spectral and morphological properties of bioparticles are challenging. There is therefore a need for the parallel acquisition and interpretation of spatially and spectrally resolved measurements with which particles can be characterized and classified in-flow with high throughput. Therefore, in this paper we investigated the scientific and technological connectivity of standard imaging flow cytometry (IFC) with filter-on-chip based spatially and spectrally resolving snapshot-mosaic cameras for photonic sensing and control in a smart and innovative microfluidic device. For the investigations presented here we used the microalgae Haematococcus pluvialis (HP). These microalgae are used commercially to produce the antioxidant keto-carotenoid astaxanthin. Therefore, HP is relevant to practically demonstrate the usability of the developed system for Multispectral Imaging Flow Cytometry (MIFC) platform. The extension of standard IFC with snapshot-mosaic cameras and multivariate data processing is an innovative approach for the in-flow characterization and derived classification of bioparticles. Finally, the multispectral data acquisition and the therefore developed methodology is generalizable and enables further applications far beyond the here characterized population of HP cells

Bis{1-[2-(diphenyl­phosphino)­ethyl]-3-ethylimidazol-2-yl­idene}palladium(II) bis­(hexa­fluoridophosphate) acetonitrile 2.85-solvate

In the structure of the title compound, [Pd(C19H21N2P)2](PF6)2·2.85CH3CN, the two six-membered NHC-phosphane chelate rings form a distorted square-planar coordination geometry around the PdII atom, which lies 0.212 (1) Å above the coordination plane. The sum of the bond angles at PdII is 358.3°, with C—Pd—P bite angles of 84.03 (10) and 83.54 (9)°. The structure includes three acetonitrile solvent mol­ecules, one with partial site occupation and one with severe disorder, which was eventually excluded from the refinement

N-Trityl-2-(tritylsulfan­yl)aniline

The title compound, C44H35NS, is a derivative of amino­thio­phenol and possesses a protected S-triphenyl­methyl thio­ether and an N-triphenyl­methyl­amine functional group. The trityl groups show an anti orientation, with C—C—N—C and C—C—S—C torsion angles of −151.0 (3) and −105.3 (2)°, respectively. There is an intra­molecular N—H⋯S hydrogen bond

2-[2-(Benzyl­sulfan­yl)phen­yl]-1,1,3,3-tetra­methyl­guanidine

The mol­ecular structure of the title compound, C18H23N3S, shows it to be a derivative of an amino­thio­phenol possessing a tetra­methyl­guanidine group with a localized C=N double bond of 1.304 (2) Å and a protected thiol functional group as an S-benzyl thio­ether. The two aromatic ring planes make a dihedral angle of 67.69 (6)°

Jet- and Wind-Driven Ionized Outflows in the Superbubble and Star-Forming Disk of NGC 3079

HST WFPC2 images are presented that span the inner 19 kpc diameter of the edge-on spiral galaxy NGC 3079; they are combined with ground-based Halpha+[N II] Fabry-Perot spectra and VLA images of radio polarization vectors and rotation measures. Ionized gas filaments within 9 kpc diameter project ~3 kpc above the disk, with the brightest forming the 1 kpc diameter superbubble. They are often resolved into strands ~0."3 (25 pc) wide which emerge from the nuclear CO ring as five distinct gas streams with velocity gradients and dispersions of hundreds of km/s. One stream flows for 250 pc and aligns with the VLBI-scale radio jet, the other four are not connected to the jet, instead curving to the vertical 0.6 kpc above the galaxy disk, then dispersing in a spray of droplets each with ionized mass ~1000 sqrt(f) Msun (volume filling factor f > 0.003 from our data). Shredded clumps of disk gas form a similar structure in hydro models of a galaxy-scale wind. The pattern of magnetic fields and the gas kinematics also suggest a wind of mechanical luminosity 10^43 erg/s that has stagnated in the galaxy disk at radius ~800 pc, flared to larger radii with increasing height as the balancing ISM pressure reduces above the disk, and entrained dense clouds into a vortex. Total KE and momentum of the filaments are (0.4-5)x10^55 sqrt(f) ergs and (1.6-6)x10^47 sqrt(f) dyne s. A star-forming complex elsewhere in the galaxy shows a striking spray of linear filaments that extend for hundreds of parsecs.Comment: Accepted to ApJ, 31 pages, 15 figures (some color). High-fidelity figs at http://www.physics.unc.edu/~cecil/science/n3079.htm

Novel targeted therapies for eosinophilic disorders

Hypereosinophilic syndromes (HESs) are a diverse group of conditions characterized by clinical manifestations attributable to eosinophilia and eosinophilic infiltration of tissues. HESs are chronic disorders with significant morbidity and mortality. Although the availability of targeted chemotherapeutic agents, including imatinib, has improved quality of life and survival in some patients with HESs, additional agents with increased efficacy and decreased toxicity are sorely needed. The purpose of this review is to provide an overview of eosinophil biology with an emphasis on potential targets of pharmacotherapy and to provide a summary of potential eosinophil-targeting agents, including those in development, in clinical trials, or approved for other disorders

The TOP-SCOPE Survey of Planck Galactic Cold Clumps : Survey Overview and Results of an Exemplar Source, PGCC G26.53+0.17

The low dust temperatures (<14 K) of Planck Galactic cold clumps (PGCCs) make them ideal targets to probe the initial conditions and very early phase of star formation. "TOP-SCOPE" is a joint survey program targeting similar to 2000 PGCCs in J = 1-0 transitions of CO isotopologues and similar to 1000 PGCCs in 850 mu m continuum emission. The objective of the "TOP-SCOPE" survey and the joint surveys (SMT 10 m, KVN 21 m, and NRO 45 m) is to statistically study the initial conditions occurring during star formation and the evolution of molecular clouds, across a wide range of environments. The observations, data analysis, and example science cases for these surveys are introduced with an exemplar source, PGCC G26.53+0.17 (G26), which is a filamentary infrared dark cloud (IRDC). The total mass, length, and mean line mass (M/L) of the G26 filament are similar to 6200 M-circle dot, similar to 12 pc, and similar to 500 M-circle dot pc(-1), respectively. Ten massive clumps, including eight starless ones, are found along the filament. The most massive clump as a whole may still be in global collapse, while its denser part seems to be undergoing expansion owing to outflow feedback. The fragmentation in the G26 filament from cloud scale to clump scale is in agreement with gravitational fragmentation of an isothermal, nonmagnetized, and turbulent supported cylinder. A bimodal behavior in dust emissivity spectral index (beta) distribution is found in G26, suggesting grain growth along the filament. The G26 filament may be formed owing to large-scale compression flows evidenced by the temperature and velocity gradients across its natal cloud.Peer reviewe

Untersuchungen ueber S-H...S - Wasserstoffbrueckenbindungen in Thiosaeuren des Phosphors und des Kohlenstoffs

Henkel G. Untersuchungen ueber S-H..S - Wasserstoffbrueckenbindungen in Thiosaeuren des Phosphors und des Kohlenstoffs. Bielefeld; 1976