Utilização de terapia fotodinâmica e óleos essenciais no controle In Vitro de Acinetobacter Baumannii e Pseudomonas Aeruginosa

O aumento da incidência de bactérias resistentes às múltiplas classes de antimicrobianos, principalmente Gram-negativas de prevalência nosocomial, têm se mostrado como grande ameaça à saúde pública internacional. O desenvolvimento de terapias alternativas que não induzem resistência bacteriana se mostra necessária e promissora. Este estudo objetivou avaliar a ação de extratos naturais derivados de plantas e terapia fotodinâmica no controle Pseudomonas aeruginosa e Acinetobacter baumannii cultivadas in vitro, através da determinação de concentrações inibitórias e bactericidas mínimas, e quantificação de unidades formadoras de colônias após exposição dos respectivos agentes, variando em tempo e concentração. Evidenciou-se ação dos extratos de limão taiti, tangerina e laranja amarga em diferentes concentrações. Neste estudo, a tangerina (Citrus tangerina) causou efeito inibitório e bactericida com menor quantidade em ambas as bactérias quando comparada aos outros extratos, porém, com maior concentração necessária na A. baumannii quando comparada à P. aeruginosa. Em relação à terapia fotodinâmica, foi evidenciada redução expressiva na quantidade de bactérias existentes no ambiente exposto, principalmente por maior quantidade de tempo, porem, sem garantir efeito inativação total de tais bactérias. A atividade antimicrobinada evidenciada no estudo, de ambas as terapias, demonstra a possibilidade do desenvolvimento de agentes adjuvantes no controle de replicação bacteriana com baixo custo e boa eficácia, sem risco de resistência bacteriana adicional

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Diretriz da SBC sobre Diagnóstico e Tratamento de Pacientes com Cardiomiopatia da Doença de Chagas – 2023

Note: These guidelines are for information purposes and should not replace the clinical judgment of a physician, who must ultimately determine the appropriate treatment for each patient

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved