A photometric and kinematic study of the stars and interstellar medium in the central two kpc of NGC 3379

HST images of NGC 3379 show that the V and I luminosity profiles in the inner 13 arcsec of this E1 galaxy are represented by two different components: a stellar bulge following a Sersic Law with exponent n = 2.36, and a central core (r < 0.7 arcsec) with a characteristic "cuspy" profile. Subtraction of the underlying stellar component represented by the fitted Sersic profile revealed the presence of a small (r ~ 105 pc) dust disk of about 150 solar masses, oriented at PA = 125 degrees and inclined ~ 77 degrees with respect to the line of sight. The same absorption structure is detected in the color-index (V-I) image. The stellar rotation in the inner 20 arcsec is well represented by a parametric planar disk model, inclined ~ 26 degrees relative to the plane of the sky, and apparent major axis along PA ~ 67 degrees. The gas velocity curves in the inner 5 arcsec show a steep gradient, indicating that the gas rotates much faster than the stars, although in the same direction. The velocity field of the gaseous system, however, is not consistent with the simple model of Keplerian rotation sustained by the large (7 x 10E9 solar masses within a radius of ~ 90 pc) central mass implied by the maximum velocity observed, but the available data precludes a more detailed analysis.Comment: 23 pages, LaTeX(aaspp4.sty), 9 figures included. Figs. 1 and 5 are colour plates. Accepted for publication in The Astrophysical Journal (part 1

Specifications for an inductively coupled plasma simultaneous multielement analysis system

Specifications are presented for those items of hardware, software, and overall system performance which are of known importance in atomic emission spectroscopy--simultaneous multielement analyses (ICP-SMA) instrument systems. Particular attention is directed to the specified data reporting and quality assurance features which are required for improving the validity and interpretability of the analytical results, and the specified background correction procedures which are necessary for accurate quantitative determinations near the limit of detection. The specification package was prepared to specify and purchase an ICP-SMA system which will provide maximum value and utility for the simultaneous determination of major, minor and trace quantities of the elements in a wide variety of sample materials. Modifications of the analytical line array specified here may be appropriate for other, more specific, analytical tasks. Budgetary realities may necessitate additional, appropriate modifications. It is anticipated, however, that the specification package presented here will have general utility as a guide in the preparation of ICP-SMA procurement packages for other laboratories and other analytical applications

Primary structure and spectroscopic studies of Neurospora copper metallothionein.

When Neurospora crassa is grown in the presence of Cu(II) ions, it accumulates the metal with the concomitant synthesis of a low molecular weight copper-binding protein. The molecule binds 6 g-atom of copper per mole protein (Mr = 2200) and shows a striking sequence homology to the zinc- and cadmium-binding vertebrate metallothioneins. Absorption, circular dichroism, and electron paramagnetic resonance spectroscopy of Neurospora metallothionein indicate the copper to be bound to cysteinyl residues as a Cu(I)-thiolate complex of the polymeric mu-thiolate structure [Cu(I)6RS7]-. This metal-binding mode is also in agreement with the unusual luminescence of the protein. Spectral perturbation studies with HgCl2 and p-(chloromercuri)benzoate suggest that the 6 Cu(I)ions are coordinated to the seven cysteinyl residues in the form of a single metal cluster. Neurospora apometallothionein is also capable of binding in vivo group IIB metal ions [Zn(II), Cd(II), and Hg(II)] as well as paramagnetic Co(II) ions with an overall metal-to-protein stoichiometry of 3. The spectroscopic properties of the fully substituted forms are indicative of a distorted tetrahedral coordination. However, metal titration of the apoprotein shows the third metal ion to be differently coordinated than the other two metal ions. This difference can be explained by the presence of only seven cysteine residues in Neurospora metallothionein as opposed to nine cysteine residues in the three-metal cluster of the mammalian metallothioneins

The origin of the Narrow Line Region of Mrk 3: an overpressured jet cocoon

We have obtained HST FOC long-slit optical spectroscopy of the Narrow Line Region of the Seyfert 2 galaxy Mrk 3. In the region cospatial with the radio-jet the velocity field is highly perturbed and shows two velocity systems separated by as much as 1700 km/s. We interpret this to be the consequence of the rapid expansion of a cocoon of hot gas, shocked and heated by the radio-emitting outflow, which compresses and accelerates the ambient gas. The NLR itself is essentially a cylindrical shell expanding supersonically. From the size and velocity of the expanding region, we derive an upper limit to the radio-source age, ~ 2 E42 erg/s required to inflate the cocoon and estimate that the jet minimum advance speed is 3 E-3 pc per year. The total kinetic energy of the high velocity NLR gas can be estimated as ~6 E54 erg, comparable to the total energy carried by the jet over its lifetime and this quantitatively supports the idea that the NLR gas is accelerated by the jet. If the advance speed of Mrk 3 is representative of the Seyfert population then these sources must also be short lived and probably recurrent. The jet kinetic luminosity of Mrk 3 is between 2 and 3 orders of magnitude smaller than that derived for radio-loud AGNs with similar emission-line luminosity. On the other hand, the fraction of jet power dissipated in radio-emission is similar. We speculate that the main distinction between radio-quiet and radio-loud AGN is ascribed to a difference in jet power rather than to a different efficiency in synchrotron emission production.Comment: 13 pages, 8 figures, Astrophysical Journal in pres

Reverberation Mapping and the Physics of Active Galactic Nuclei

Reverberation-mapping campaigns have revolutionized our understanding of AGN. They have allowed the direct determination of the broad-line region size, enabled mapping of the gas distribution around the central black hole, and are starting to resolve the continuum source structure. This review describes the recent and successful campaigns of the International AGN Watch consortium, outlines the theoretical background of reverberation mapping and the calculation of transfer functions, and addresses the fundamental difficulties of such experiments. It shows that such large-scale experiments have resulted in a ``new BLR'' which is considerably different from the one we knew just ten years ago. We discuss in some detail the more important new results, including the luminosity-size-mass relationship for AGN, and suggest ways to proceed in the near future.Comment: Review article to appear in Astronomical Time Series, Proceedings of the Wise Observatory 25th Ann. Symposium. 24 pages including 7 figure

Orbit-Based Dynamical Models of the Sombrero Galaxy (NGC 4594)

We present axisymmetric, orbit-based models to study the central black hole, stellar mass-to-light ratio, and dark matter halo of NGC 4594 (M104, the Sombrero Galaxy). For stellar kinematics, we use published high-resolution kinematics of the central region taken with the Hubble Space Telescope, newly obtained Gemini long-slit spectra of the major axis, and integral field kinematics from the SAURON instrument. At large radii, we use globular cluster kinematics to trace the mass profile and apply extra leverage to recovering the dark matter halo parameters. We find a black hole of mass M_{\bullet}=(6.6 +/- 0.4) x 10^8 M_{\odot}, and determine the stellar M/L_I=3.4 +/- 0.05 (uncertainties are the 68% confidence band marginalized over the other parameters). Our best fit dark matter halo is a cored logarithmic model with asymptotic circular speed V_c=376 +/- 12 km/s and core radius r_c= 4.7 +/- 0.6 kpc. The fraction of dark to total mass contained within the half-light radius is 0.52. Taking the bulge and disk components into account in our calculation of \sigma_e puts NGC 4594 squarely on the M-\sigma relation. We also determine that NGC 4594 lies directly on the M-L relation.Comment: 13 pages, 10 figures, accepted for publication in Ap

Filaggrin Genotype Determines Functional and Molecular Alterations in Skin of Patients with Atopic Dermatitis and Ichthyosis Vulgaris

BACKGROUND: Several common genetic and environmental disease mechanisms are important for the pathophysiology behind atopic dermatitis (AD). Filaggrin (FLG) loss-of-function is of great significance for barrier impairment in AD and ichthyosis vulgaris (IV), which is commonly associated with AD. The molecular background is, however, complex and various clusters of genes are altered, including inflammatory and epidermal-differentiation genes. OBJECTIVE: The objective was to study whether the functional and molecular alterations in AD and IV skin depend directly on FLG loss-of-function, and whether FLG genotype determines the type of downstream molecular pathway affected. METHODS AND FINDINGS: Patients with AD/IV (n = 43) and controls (n = 15) were recruited from two Swedish outpatient clinics and a Swedish AD family material with known FLG genotype. They were clinically examined and their medical history recorded using a standardized questionnaire. Blood samples and punch biopsies were taken and trans-epidermal water loss (TEWL) and skin pH was assessed with standard techniques. In addition to FLG genotyping, the STS gene was analyzed to exclude X-linked recessive ichthyosis (XLI). Microarrays and quantitative real-time PCR were used to compare differences in gene expression depending on FLG genotype. Several different signalling pathways were altered depending on FLG genotype in patients suffering from AD or AD/IV. Disease severity, TEWL and pH follow FLG deficiency in the skin; and the number of altered genes and pathways are correlated to FLG mRNA expression. CONCLUSIONS: We emphasize further the role of FLG in skin-barrier integrity and the complex compensatory activation of signalling pathways. This involves inflammation, epidermal differentiation, lipid metabolism, cell signalling and adhesion in response to FLG-dependent skin-barrier dysfunction

NGC6240: Merger-Induced Star Formation & Gas Dynamics

We present spatially resolved integral field spectroscopic K-band data at a resolution of 0.13" (60pc) and interferometric CO(2-1) line observations of the prototypical merging system NGC6240. Despite the clear rotational signature, the stellar kinematics in the two nuclei are dominated by dispersion. We use Jeans modelling to derive the masses and the mass-to-light ratios of the nuclei. Combining the luminosities with the spatially resolved Br-gamma equivalent width shows that only 1/3 of the K-band continuum from the nuclei is associated with the most recent star forming episode; and that less than 30% of the system's bolometric luminosity and only 9% of its stellar mass is due to this starburst. The star formation properties, calculated from typical merger star formation histories, demonstrate the impact of different assumptions about the star formation history. The properties of the nuclei, and the existence of a prominent old stellar population, indicate that the nuclei are remnants of the progenitor galaxies' bulges.Comment: 18 pages, 14 figures. Accepted for publication in A&