215 research outputs found
Effect of anti-glycosphingolipid monoclonal antibodies in pathogenic fungal growth and differentiation. Characterization of monoclonal antibody MEST-3 directed to Manpα1→3Manpα1→2IPC
<p>Abstract</p> <p>Background</p> <p>Studies carried out during the 1990's demonstrated the presence of fungal glycoinositol phosphorylceramides (GIPCs) with unique structures, some of them showed reactivity with sera of patients with histoplasmosis, paracoccidioidomycosis or aspergillosis. It was also observed that fungal GIPCs were able to inhibit T lymphocyte proliferation "in vitro", and studies regarding the importance of these molecules to fungal survival showed that many species of fungi are vulnerable to inhibitors of sphingolipid biosynthesis.</p> <p>Results</p> <p>In this paper, we describe a detailed characterization of an IgG2a monoclonal antibody (mAb), termed MEST-3, directed to the <it>Paracoccidioides brasiliensis </it>glycolipid antigen Pb-2 (Man<it>p</it>α1→3Man<it>p</it>α1→2IPC). mAb MEST-3 also recognizes GIPCs bearing the same structure in other fungi. Studies performed on fungal cultures clearly showed the strong inhibitory activity of MEST-3 on differentiation and colony formation of <it>Paracoccidioides brasiliensis</it>, <it>Histoplasma capsulatum </it>and <it>Sporothrix schenckii</it>. Similar inhibitory results were observed when these fungi where incubated with a different mAb, which recognizes GIPCs bearing terminal residues of β-D-galactofuranose linked to mannose (mAb MEST-1). On the other hand, mAb MEST-2 specifically directed to fungal glucosylceramide (GlcCer) was able to promote only a weak inhibition on fungal differentiation and colony formation.</p> <p>Conclusions</p> <p>These results strongly suggest that mAbs directed to specific glycosphingolipids are able to interfere on fungal growth and differentiation. Thus, studies on surface distribution of GIPCs in yeast and mycelium forms of fungi may yield valuable information regarding the relevance of glycosphingolipids in processes of fungal growth, morphological transition and infectivity.</p
Hematological parameters, liver integrity and growth of Nile tilapia fingerlings fed diets supplemented with propolis extract
Aim of study: To assess the effects of propolis extract supplementation in diets for Nile tilapia (Oreochromis niloticus) fingerlings on growth performance, hematological and histological parameters.
Area of study: The study was carried out in Paraná (Brazil).
Material and methods: The experimental design was based on six treatments including the control diet and propolis supplementation (2%, 4%, 6%, 8% and 10% in the diet). Three hundred Nile tilapia fingerlings, with an initial weight of 0.61± 0.02 g, were distributed in 30 plastic mesh hapas (0.15 m3 each) arranged in a concrete tank of 25 m3 of water volume. The duration of the experimental period was 90 days.
Main results: The increasing levels of propolis did not influence the growth performance and proximate composition of fishes. Red blood cells and hematological indices were not affected by propolis supplementation. However, total leukocytes and thrombocytes were higher in fish fed on propolis diets, being significant in fish fed 2% and 8%, and 6% and 8% supplemented diets groups, respectively.
Research highlights: The supplementation of propolis alcoholic extract in the range of 2, 4, 6 and 8% in the diet for Nile tilapia fingerlings promote healthier fish with increased immunity in the evaluated culture conditions
Disruption of the glucosylceramide biosynthetic pathway in Aspergillus nidulans and Aspergillus fumigatus by inhibitors of UDP-Glc : ceramide glucosyltransferase strongly affects spore germination, cell cycle, and hyphal growth
The opportunistic mycopathogen Aspergillus fumigatus expresses both glucosylceramide and galactosylceramide (GlcCer and GalCer), but their functional significance in Aspergillus species is unknown. We here identified and characterized a GlcCer from Aspergillus nidulans, a non-pathogenic model fungus. Involvement of GlcCer in fungal development was tested on both species using a family of compounds known to inhibit GlcCer synthase in mammals. Two analogs, D-threo-1-phenyl-2-palmitoyl-3-pyrrolidinopropanol (P4) and D-threo-3',4'-ethylenedioxy-P4, strongly inhibited germination and hyphal growth. Neutral lipids from A. fumigatus cultured in the presence of these inhibitors displayed a significantly reduced GlcCer/GalCer ratio. These results suggest that synthesis of GlcCer is essential for normal development of A. fumigatus and A. nidulans. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.Univ New Hampshire, Dept Chem, Durham, NH 03824 USAUniv Georgia, Dept Bot, Athens, GA 30602 USAUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, BrazilUniv Michigan, Med Ctr, Dept Internal Med, Div Nephrol, Ann Arbor, MI 48109 USAUniv Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USAUniv Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USAUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, BrazilWeb of Scienc
Estimulação do enraizamento de estacas de seringueira (Hevea brasiliensis Muell. Arg.) pela aplicação de reguladores vegetais
Este ensaio foi conduzido com o objetivo de verificar o efeito do ácido indolbutírico (lBA), ácido naftalenacético (NAA), ácido indolilacético (IAA) e ácido succínico-2,2-dimetilhidrazida (SADH) no enraizamento de estacas de plantas jovens de seringueira. As estacas utilizadas foram retiradas da parte inferior das plantas e tiveram suas bases imersas por 1 hora em água, ou em soluções de IBA, NAA, IAA e SADH 2500 ppm. Os resultados obtidos 77 dias após o plantio mostraram que IBA promoveu maior porcentagem de enraizamento com relação ao controle. Porém, em relação ao brotamento das estacas, o tratamento com SADH mostrou-se superior ao controle. Os tratamentos com NAA e IAA revelaram resultados inferiores ao controle em relação ao número de estacas vivas, estacas com calos e estacas com brotações.This experiment was carried out to evaluate the effect of IBA, NAA, IAA and SADH on rooting of stem cuttings of Hevea brasilien sis. The stem cuttings were taken from the base of 18 months old plants, and then immersed in water or IBA, NAA, IAA and SADH solutions at concentration of 2500 ppm, by 1 hour.Pllants treated with IBA showed higher percentage of rooting compared with check treatment. SADH promoted increase in bud break. NAA and IAA reduced the number of cuttings alive, budded and with callus formation
Ação de estimulantes vegetais no desenvolvimento de pepino (Cucumis sativus L.)
This experiment was carried out to determine the effects of Triacontanol (0.5 g/l), Ergostim (2 ml/l), Atonik (0.5 ml/l) and Agrostemmin (1.25 g/l) on growth of cucumber plants 'Hibrido Caipira AG-207'. Cucumber plants were sprayed 7 days after sowing, under greenhouse conditions. Agrostemmin reduced plant height until 20 days after sowing. Atonik reduced plant height 27 days after sowing. At 34 days after cucumber sowing, plants treated with the plant stimulants not differed in height in relation to check treatment. Agrostemmin and Ergostim increased dry matter production of the lower part of the plant. The plant stimulants does not change the dry matter production of the apical region of cucumber plants.Observou-se o efeito de quatro estimulantes vegetais no desenvolvimento de plantas de pepino 'Hibrido Caipira AG-207', em condições de casa de vegetação, tendo a semeadura sido realizada em vasos de cerâmica e as plantas pulverizadas sete dias após a semeadura, com Triacontanol (1-hidroxitriacontano) na dosagem de 0,5 g/l, Ergostim (L-cisteina e ácido fólico + izometilentramina) 2 ml/ l, Atonik (mononitroguaiacol sódico e outros compostos nitrogenados aromáticos) 0,5 ml/l e Agrostemin (alantoina + triptofano +ácido fólico + ácido glutâmico + ácido alantóico + arcialanina + outros aminoácidos) 1,25 g/l. Através dos resultados obtidos verificou-se que nenhum dos estimulantes vegetais estudados promoveu aumento em altura das plantas de pepino, embora tenham mostrado uma tendência em provocar um aumento no peso da matéria seca
Preliminary inventory of the anuran fauna of the Mata Estrela Private Natural Heritage Reserve, in the Atlantic Forest of the State of Rio Grande do Norte, Brazil
Tropical regions harbor the highest amphibian biodiversity, nevertheless, information on species composition is lacking in most areas yet these baseline data are essential to guide conservation strategies. The goal of the present study was to survey the anurans of a Private Natural Heritage Reserve named Mata Estrela, a fragment of about 26 km2 in the Northern Atlantic Forest, Brazil. The study was carried out as an active census in June 2015. We found 27 anuran species assigned to five families: Bufonidae (2 spp.), Craugastoridae (1 sp.), Hylidae (11 spp.), Leptodactylidae (12 spp.) and Phyllomedusidae (1 sp.). All species are widespread in the Atlantic forest as well as in other South American ecoregions. Descriptions of local diversity such as this one are a fundamental baseline for conservation work and are especially needed in the northern limits of the Atlantic forest, for which there is still a huge lack of biological knowledge.Asociación Herpetológica Argentin
The Fourteenth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the extended Baryon Oscillation Spectroscopic Survey and from the second phase of the Apache Point Observatory Galactic Evolution Experiment
The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in
operation since July 2014. This paper describes the second data release from
this phase, and the fourteenth from SDSS overall (making this, Data Release
Fourteen or DR14). This release makes public data taken by SDSS-IV in its first
two years of operation (July 2014-2016). Like all previous SDSS releases, DR14
is cumulative, including the most recent reductions and calibrations of all
data taken by SDSS since the first phase began operations in 2000. New in DR14
is the first public release of data from the extended Baryon Oscillation
Spectroscopic Survey (eBOSS); the first data from the second phase of the
Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2),
including stellar parameter estimates from an innovative data driven machine
learning algorithm known as "The Cannon"; and almost twice as many data cubes
from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous
release (N = 2812 in total). This paper describes the location and format of
the publicly available data from SDSS-IV surveys. We provide references to the
important technical papers describing how these data have been taken (both
targeting and observation details) and processed for scientific use. The SDSS
website (www.sdss.org) has been updated for this release, and provides links to
data downloads, as well as tutorials and examples of data use. SDSS-IV is
planning to continue to collect astronomical data until 2020, and will be
followed by SDSS-V.Comment: SDSS-IV collaboration alphabetical author data release paper. DR14
happened on 31st July 2017. 19 pages, 5 figures. Accepted by ApJS on 28th Nov
2017 (this is the "post-print" and "post-proofs" version; minor corrections
only from v1, and most of errors found in proofs corrected
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