Lifespan extension without fertility reduction following dietary addition of the autophagy activator Torin1 in Drosophila melanogaster

Autophagy is a highly conserved mechanism for cellular repair that becomes progressively down-regulated during normal ageing. Hence, manipulations that activate autophagy could increase lifespan. Previous reports show that manipulations to the autophagy pathway can result in longevity extension in yeast, flies, worms and mammals. Under standard nutrition, autophagy is inhibited by the nutrient sensing kinase Target of Rapamycin (TOR). Therefore, manipulations of TOR that increase autophagy may offer a mechanism for extending lifespan. Ideally, such manipulations should be specific and minimise off-target effects, and it is important to discover additional methods for ‘clean’ lifespan manipulation. Here we report an initial study into the effect of up-regulating autophagy on lifespan and fertility in Drosophila melanogaster by dietary addition of Torin1. Activation of autophagy using this selective TOR inhibitor was associated with significantly increased lifespan in both sexes. Torin1 induced a dose-dependent increase in lifespan in once-mated females. There was no evidence of a trade-off between longevity and fecundity or fertility. Torin1-fed females exhibited significantly elevated fecundity, but also elevated egg infertility, resulting in no net change in overall fertility. This supports the idea that lifespan can be extended without trade-offs in fertility and suggest that Torin1 may be a useful tool with which to pursue anti-ageing research

The Development and Validation of a Reliable Alternate Form for Raven's Standard Progressive Matrices

The Ravens Advanced Progressive Matrices (APM) is a widely used measure of general intelligence (g), both across settings and cultures. Due to its lengthy 40-min administration time, several researchers have developed short-form scales, yet these forms typically yield a significantly lower reliability. This article describes the creation of an 18-item short form (APM-18) and its validation in three samples of Southwestern U.S. university students (total N = 633). The APM-18 shows similar psychometric properties to both the previously published 36-item long form and 12-item short form, but retains a reliability estimate closer to the original APM. This, plus the shorter administration time (25 min) relative to the complete APM (40-60 min), makes it useful for time-constrained or mass-testing situations.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]