Cremation practices and the creation of monument complexes: the Neolithic cremation cemetery at Forteviot, Strathearn, Perth & Kinross, Scotland, and its comparanda

Around the beginning of the 3rd millennium cal bc a cremation cemetery was established at Forteviot, central Scotland. This place went on to become one of the largest monument complexes identified in Mainland Scotland, with the construction of a palisaded enclosure, timber structures, and a series of henge monuments and other enclosures. The cemetery was established between 3080 and 2900 cal bc, probably in the 30th century cal bc, which is contemporary with the cremation cemetery at Stonehenge. Nine discrete deposits of cremated bone, representing the remains of at least 18 people, were identified. In most instances they were placed within cut features and, in one case, a series of cremation deposits was associated with a broken standing stone. This paper includes the first detailed assessment of the cremated remains at Forteviot and the features associated with the cemetery, and explores how the establishment of this cemetery may have been both a catalyst and inspiration for the elaborate monument building and prolonged acts of remembrance that occurred at this location over a period of almost 1000 years. The paper also outlines the parallels for Forteviot across Britain and, for the first time, draws together the dating evidence (including Bayesian modelling) for this major category of evidence for considering the nature of late 4th/early 3rd millennium cal bc society. The results and discussion have wide implications and resonances for contemplating the establishment and evolution of monument complexes in prehistoric Britain and beyond

Compromised barrier integrity of human feto-placental vessels from gestational diabetic pregnancies is related to downregulation of occludin expression

© 2020, The Author(s). Aims/hypothesis: Reduced occupancy of junctional occludin is a feature of human placental vessels in the diabetic milieu. However, the functional consequence of this and whether this loss is due to differential expression of occludin splice variants is not known. Our study aimed to investigate the effects of gestational diabetes mellitus (GDM), and its treatment, on endothelial junctional integrity, gene and protein expression of occludin splice variants, and potential regulation of expression by microRNAs (miRNAs). Methods: Term placentas were obtained from normal pregnancies (n = 21), and pregnancies complicated by GDM where glucose levels were controlled by diet (n = 11) or metformin (n = 6). Gene and microRNA (miRNA) expression were determined by quantitative real-time PCR; protein expression by immunoblotting; endothelial junctional occupancy by fluorescence microscopy and systematic sampling; and paracellular leakage by perfusion of placental microvascular beds with 76 Mr dextran. Transfection studies of miRNAs that target OCLN were performed in HUVECs, and the trans-endothelial electrical resistance and tracer permeability of the HUVECs were measured. Results: All three predicted OCLN gene splice variants and two occludin protein isoforms were found in human placental samples. In placental samples from diet-controlled GDM (d-GDM) pregnancies we found a lower percentage of conduit vessels showing occludin immunoreactivity (12%, p < 0.01), decreased levels of the fully functional occludin isoform-A protein (29%), and differential gene expression of OCLN variant 2 (33% decrease), variant 3 (3.3-fold increase). These changes were not seen in samples from the group with metformin-controlled GDM. In d-GDM placentas, increased numbers of conduit microvessels demonstrated extravasation of 76 Mr dextran (2.0-fold). In d-GDM expression of one of the five potential miRNAs targeting OCLN, miR-181a-5p, expression was 2.1-fold that in normal pregnancies. Experimental overexpression of miR-181a-5p in HUVECs from normal pregnancies resulted in a highly significant downregulation of OCLN variant 1 (69%) and variant 2 (46%) gene expression, with decreased trans-endothelial resistance (78%) and increase in tracer permeability (1.3-fold). Conclusions/interpretation: Downregulation of expression of OCLN variant 2 and the fully functional occludin isoform-A protein are a feature of placentas in d-GDM pregnancies. These may be behind the loss of junctional occludin and the increased extravasation of exogenous dextran observed. miR-181a-5p was in part responsible for the downregulation of occludin in placentas from d-GDM pregnancies. Induced overexpression of miR-181a-5p compromised the integrity of the endothelial barrier. Our data suggest that, despite good glucose control, the adoption of lifestyle changes alone during a GDM pregnancy may not be enough to prevent an alteration in the expression of occludin and the subsequent functional consequences in placentas and impaired vascular barrier function in offspring. [Figure not available: see fulltext.

Cremation Practices and the Creation of Monument Complexes : The Neolithic Cremation Cemetery at Forteviot, Strathearn, Perth & Kinross, Scotland, and its comparanda

This paper is the result of the hard work and contribution of many people. In particular we would like to thank the substantial contributions made to this paper by Alison Sheridan, Stephany Leach and Derek Hamilton; they also kindly read over and commented on earlier versions of this paper, but all errors remain our own. The excavations at Forteviot were the work of dozens of students from Glasgow and Aberdeen Universities and local volunteers. Dupplin Estate kindly allowed us to work at Forteviot. Fieldwork and post-excavation analysis was largely funded by Historic Environment Scotland (then Historic Scotland), and we very much benefited from the support of colleagues within the SERF Project – Ewan Campbell, Steve Driscoll and Tessa Poller. Figures 1 and 2 were prepared by Lorraine McEwan, while Figures 3 and 16 were drawn by Alison Sandison. Figures 11 to 15 were prepared by Derek Hamilton, while the drawings in Figures 7 and 8 were undertaken by Marion O’Neil. Historic Environment Scotland have provided grant aid for the publication and illustration of this paper.Peer reviewedPublisher PD

The Beaker phenomenon and the genomic transformation of northwest Europe

From around 2750 to 2500 bc, Bell Beaker pottery became widespread across western and central Europe, before it disappeared between 2200 and 1800 bc. The forces that propelled its expansion are a matter of long-standing debate, and there is support for both cultural diffusion and migration having a role in this process. Here we present genome-wide data from 400 Neolithic, Copper Age and Bronze Age Europeans, including 226 individuals associated with Beaker-complex artefacts. We detected limited genetic affinity between Beaker-complex-associated individuals from Iberia and central Europe, and thus exclude migration as an important mechanism of spread between these two regions. However, migration had a key role in the further dissemination of the Beaker complex. We document this phenomenon most clearly in Britain, where the spread of the Beaker complex introduced high levels of steppe-related ancestry and was associated with the replacement of approximately 90% of Britain’s gene pool within a few hundred years, continuing the east-to-west expansion that had brought steppe-related ancestry into central and northern Europe over the previous centuries

Going underground : an anthropological and taphonomic study of human skeletal remains from caves and rock shelters in Yorkshire

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