Menstrual cycle-associated modulations in neuromuscular function and fatigability of the knee extensors in eumenorrheic women

Sex hormone concentrations of eumenorrheic women typically fluctuate across the menstrual cycle and can affect neural function such that estrogen has neuroexcitatory effects, and progesterone induces inhibition. However, the effects of these changes on corticospinal and intracortical circuitry and the motor performance of the knee extensors are unknown. The present two-part investigation aimed to 1) determine the measurement error of an exercise task, transcranial magnetic stimulation (TMS)-, and motor nerve stimulation (MNS)-derived responses in women ingesting a monophasic oral contraceptive pill (hormonally-constant) and 2) investigate whether these measures were modulated by menstrual cycle phase (MCP), by examining them before and after an intermittent isometric fatiguing task (60% of maximal voluntary contraction, MVC) with the knee extensors until task failure in eumenorrheic women on days 2, 14, and 21 of the menstrual cycle. The repeatability of neuromuscular measures at baseline and fatigability ranged between moderate and excellent in women taking the oral contraceptive pill. MVC was not affected by MCP (P = 0.790). Voluntary activation (MNS and TMS) peaked on day 14 (P = 0.007 and 0.008, respectively). Whereas corticospinal excitability was unchanged, short-interval intracortical inhibition was greatest on day 21 compared with days 14 and 2 (P < 0.001). Additionally, time to task failure was longer on day 21 than on both days 14 and 2 (24 and 36%, respectively, P = 0.030). The observed changes were larger than the associated measurement errors. These data demonstrate that neuromuscular function and fatigability of the knee extensors vary across the menstrual cycle and may influence exercise performance involving locomotor muscles. NEW & NOTEWORTHY The present two-part study first demonstrated the repeatability of transcranial magnetic stimulation- and electrical motor nerve stimulation-evoked variables in a hormonally constant female population. Subsequently, it was demonstrated that the eumenorrheic menstrual cycle affects neuromuscular function. Changing concentrations of neuroactive hormones corresponded to greater voluntary activation on day 14, greater intracortical inhibition on day 21, and lowest fatigability on day 21. These alterations of knee extensor neuromuscular function have implications for locomotor activities

Technology Learning Curves for Energy Policy Support

The European Commission's Joint Research Centre and the Energy Research Centre of the Netherlands (ECN) organised an expert workshop on 'Learning Curves for Policy Support' in Amsterdam on 8 March 2012. It aimed to assess the challenges in the application of the two-factor learning curve, or alternative solutions in supporting policy decision making in the framework of the European Strategic Energy Technology Plan, and explored options for improvement. The workshop gathered distinguished experts in the field of scientific research on learning curves and policy researchers from the European Commission and ECN to assess the challenges in the application of the two-factor-learning curve, or alternative solutions in supporting policy decision making, and to provide options for improvement. This paper forms the summary of outcomes from the workshop. Due to the very different nature of the One-Factor-Learning concept and the Two-Factor-Learning concept, these are discussed in separate parts. In each of these parts the context and the methodology are introduced, methodological and data challenges are described and the problems associated with the application of the concept in models is discussed.JRC.F.6-Energy systems evaluatio

RESCUR : surfing the waves - a resilience curriculum for early years and primary schools - a teacher's guide

This book is based on a European Project, RESCUR, financed by the EU LLP Comenius Programme, together with the six Universities taking part in the project, namely the University of Malta (coordinator), University of Crete, Greece, University of Lisbon, Portugal, University of Pavia, Italy, Orebro University, Sweden, and the University of Zagreb, Croatia. The Maltese version of this book is also available in this section.RESCUR Surfing The Waves is a resilience programme for early years and primary schools in Europe developed by six European universities The curriculum seeks to empower vulnerable children at risk of early school leaving, absenteeism, disengagement, bullying, social exclusion and marginalisation through a universal, whole school approach. Amongst its special features, it includes story telling making use of two specially created animal characters, mindfulness activities at the beginning of each session, ready made activities and resources for the classroom teacher, interactive multisensory activities, learners portfolio, take home activities, teacher and self assessment checklists for each theme and subtheme, finger and cloth puppets, theme posters, and activity sheets. The activities are experiential, spiral, developmental, inclusive and make use of the SAFE approach. The programme consists of a Teachers Guide, a Parents Guide, and three manuals of activities and resources for Early Years, Early Primary Years and Late Primary Years respectively. It is available in hard and soft copies and available in English and six other languages (Croatian, Greek, Italian, Maltese, Portugese and Swedish). The Maltese version of this book is also available in this section.peer-reviewe

Systematic Analysis of FKBP Inducible Degradation Domain Tagging Strategies for the Human Malaria Parasite Plasmodium falciparum

Targeted regulation of protein levels is an important tool to gain insights into the role of proteins essential to cell function and development. In recent years, a method based on mutated forms of the human FKBP12 has been established and used to great effect in various cell types to explore protein function. The mutated FKBP protein, referred to as destabilization domain (DD) tag when fused with a native protein at the N- or C-terminus targets the protein for proteosomal degradation. Regulated expression is achieved via addition of a compound, Shld-1, that stabilizes the protein and prevents degradation. A limited number of studies have used this system to provide powerful insight into protein function in the human malaria parasite Plasmodium falciparum. In order to better understand the DD inducible system in P. falciparum, we studied the effect of Shld-1 on parasite growth, demonstrating that although development is not impaired, it is delayed, requiring the appropriate controls for phenotype interpretation. We explored the quantified regulation of reporter Green Fluorescent Protein (GFP) and luciferase constructs fused to three DD variants in parasite cells either via transient or stable transfection. The regulation obtained with the original FKBP derived DD domain was compared to two triple mutants DD24 and DD29, which had been described to provide better regulation for C-terminal tagging in other cell types. When cloned to the C-terminal of reporter proteins, DD24 provided the strongest regulation allowing reporter activity to be reduced to lower levels than DD and to restore the activity of stabilised proteins to higher levels than DD29. Importantly, DD24 has not previously been applied to regulate proteins in P. falciparum. The possibility of regulating an exported protein was addressed by targeting the Ring-Infected Erythrocyte Surface Antigen (RESA) at its C-terminus. The tagged protein demonstrated an important modulation of its expression.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)National Council for Scientific and Technological Development (CNPq)National Council for Scientific and Technological Development (CNPq)National Health and Medical Research Council of AustraliaNational Health and Medical Research Council of Australi

Contemporary Tendencies in Mediation

Editors: Humberto dalla Bernardina de Pinho, Juliana Loss de AndradePresentation / Humberto Dalla Bernardina de Pinho, Juliana Loss de Andrade. -- Mediation in England / Neil Andrews. -- Un Reto para la Mediación: el Diseño de su Código Deontológico / Nuria Belloso Martín. -- Alternative Dispute Resolution and Aboriginal-Crown Reconciliation in Canada / Roshan Danesh, Jessica Dickson. -- A False ‘Prince Charming’ Keeps ‘Sleeping Beauty’ in a Coma: On Voluntary Mediation Being the True Oxymoron of Dispute Resolution Policy / Giuseppe De Palo. -- Programa de Derivación Judicial en Puerto Rico Desde la Perspectiva de la Mediación / Jacqueline N. Font-Guzmán. -- Mediation in Switzerland / Isabelle Hering. -- Reconocimiento y eficacia de los acuerdos de mediación mercantil internacional / Juliana Loss de Andrade. -- The Uses of Mediation / Lela P. Love, Joseph B. Stulberg. -- Multi-Dimensional Mediation / Paul E. Mason. -- “Italy Is Doing It – Should We Be?” Civil and Commercial Mediation in Italy / Giovanni Matteucci. -- Limites dos Meios Alternativos de Conflito / José Marinho Paulo Junior. -- New Perspectives of Civil and Commercial Mediation in Brazil / Humberto Dalla Bernardina de Pinho. -- Practical Impacts of Theoretical Lenses / Elton Simoes, Andrea Maia. -- Development and Resistance in South Europe Justice Systems to Restorative Justice / Helena Soleto Muño

Macaque models of human infectious disease.

Macaques have served as models for more than 70 human infectious diseases of diverse etiologies, including a multitude of agents-bacteria, viruses, fungi, parasites, prions. The remarkable diversity of human infectious diseases that have been modeled in the macaque includes global, childhood, and tropical diseases as well as newly emergent, sexually transmitted, oncogenic, degenerative neurologic, potential bioterrorism, and miscellaneous other diseases. Historically, macaques played a major role in establishing the etiology of yellow fever, polio, and prion diseases. With rare exceptions (Chagas disease, bartonellosis), all of the infectious diseases in this review are of Old World origin. Perhaps most surprising is the large number of tropical (16), newly emergent (7), and bioterrorism diseases (9) that have been modeled in macaques. Many of these human diseases (e.g., AIDS, hepatitis E, bartonellosis) are a consequence of zoonotic infection. However, infectious agents of certain diseases, including measles and tuberculosis, can sometimes go both ways, and thus several human pathogens are threats to nonhuman primates including macaques. Through experimental studies in macaques, researchers have gained insight into pathogenic mechanisms and novel treatment and vaccine approaches for many human infectious diseases, most notably acquired immunodeficiency syndrome (AIDS), which is caused by infection with human immunodeficiency virus (HIV). Other infectious agents for which macaques have been a uniquely valuable resource for biomedical research, and particularly vaccinology, include influenza virus, paramyxoviruses, flaviviruses, arenaviruses, hepatitis E virus, papillomavirus, smallpox virus, Mycobacteria, Bacillus anthracis, Helicobacter pylori, Yersinia pestis, and Plasmodium species. This review summarizes the extensive past and present research on macaque models of human infectious disease

Kurikul otpornosti za rane godine i osnovnu školu u Europi: Poboljšavanje kvalitetnog obrazovanja

About twenty percent of school children experience social, emotional and behaviour problems during the course of any given year and may need the use of mental health services. The number may rise to up to fifty percent amongst children coming from socio-economically disadvantaged areas and from vulnerable communities. The economic crisis which Europe is undergoing at the moment has exacerbated the risks among those already facing disadvantages such as unemployment of young people and new families, increasing poverty and social disadvantage for the whole communities and regions. These challenges underline the need to equip children from an early age with the requisite skills to help them overcome the challenges and obstacles they are set to face in such circumstances while providing healthy and protective contexts which promote their health and well-being. This paper describes the development of a resilience curriculum for children in early years and primary schools in Europe with the aim of enhancing quality education for all children, including the most vulnerable ones. It presents and discusses the curriculum framework developed from the existing literature, including the key principles, processes and themes underlying the curriculum.Oko dvadeset posto školske djece ima socijalne, emocionalne i druge probleme u ponašanju zbog čega bi im mogla zatrebati pomoć stručnjaka za mentalno zdravlje. Taj bi se postotak mogao povećati i do pedeset posto u djece lošijeg socioekonomskog statusa ili djece koja dolaze iz posebno osjetljivih zajednica. Ekonomska kriza kojom je Europa trenutno pogođena dodatno je povećala rizik među onima koji su već od prije bili suočeni s problemima poput nezaposlenosti mladih pojedinaca i obitelji, povećavanja siromaštva i socijalne nesigurnosti cijelih zajednica i regija. Ti izazovi povećavaju potrebu da se djeci već od rane dobi pruže potrebne vještine koje će im pomoći da prevladaju izazove i prepreke s kojima se u tim okolnostima suočavaju, pružajući im u isto vrijeme zdrav i zaštitnički kontekst koji će promicati njihovo zdravlje i dobrobit. U ovom je radu opisan razvoj kurikula otpornosti za djecu predškolske i osnovnoškolske dobi u Europi s ciljem poboljšanja kvalitetnog obrazovanja sve djece, uključujući i najranjivije skupine. U radu se raspravlja o okviru kurikula razvijenog iz postojeće literature, uključujući ključna načela, procese i teme koje su mu u podlozi