Nutritional Profile of Amaranth Grain Varieties Grown in Kenya

Amaranth is an indigenous plant known for its leafy vegetables and grain. Efforts are increasingly being put forward toward increasing production and utilization of the crop. However there are gaps in knowledge and technology on nutritional diversity of different varieties grown in Kenya. The objective of this study was to finger print the nutritional diversity of grains from four (A. hypochondriacus, A albus, A. cruentus, and A. hybridus) different varieties grown in Kenya. Field evaluation was done by planting the varieties in the University farm. The composition of the grains was determined using recognized standard methods. There was no significant difference (P?0.05) in composition of raw amaranth grain varieties. On average, amaranth grains were found to be rich in proteins 15.8%, lipids 7.5%, carbohydrate 66.0%, ash 3.3% and fiber 6.9%. The profile of amino acids and the specific content did not vary among different varieties. The highest amino acid on average was glutamic acid 7.2 (g/16 g of N) followed by aspartic acid 1.7 (g/16 g of N) and threonine 1.3 (g/16 g of N). Lysine occurred in appreciable amounts. The oil extracted from amaranth grain contained mainly unsaturated fatty acids. The predominant acids in the oil were oleic, linoleic and palmitic. Total unsaturated acids ranged from 76.2% to 77.6% and saturated fatty acids 22.4% to 22.8%. Linolenic acid was present in low concentration. The grains contain a high amount of iron (18.2 mg/100g), manganese (6.1 mg/100g) and zinc (3.8 mg/100g) among other minerals. The average level of anti-nutrients in grains were; phytate (254mg/100g), tannic acid (164mg/100g) and oxalate (194mg/100g) which are within levels that can be tolerated by the body system. This indicates that amaranth grain could be one of the pathways towards solving the macro - and micronutrient deficiencies experienced in Sub- Sahara Africa. Keywords: Amaranth grain varieties, Nutritional diversity

Physical and Cooking Properties of Two Varieties of Bio-Fortified Common Beans (Phaseolus Vulgaris. L) Grown in DR Congo

Common bean (Phaseolus vulgaris L.) is the most widely grown edible legume species in DR Congo but nevertheless, different varieties vary with respect to their physical, chemical aspects and cooking properties which affect the consumer acceptability. This study evaluated the physical properties and cooking time of two varieties of bio-fortified common beans (HM21_7 and Namulenga), destined for both direct consumption and processing. The parameters assessed included the dimensions, the soaking characteristics, the density characteristics, Colour and the Hardness after cooking. The beans were soaked in distilled water and in 0.025N Na2CO3 solution for 16h then subjected to different test as mention above. The data collected were subjected to Analysis of Variance (ANOVA) using Statistix version 8.1 software. Treatment means were separated using LSD at 0.05 probability level.  HM21_7 was larger in size and 100 seeds weight with (36.27±1.33 g) compared to Namulenga (32.77±0.55 g). The sphericity was (54.4% and 58.9%), surface area was (127 mm2 and 118 mm2), aspect ratio was (0.49 and 0.55) and volume was (147 mm2 and 142 mm2) for both HM21_7 and Namulenga respectively. The soaking solutions had a significant effect on the hydration coefficient, swelling coefficient. Soaking the beans in Na2CO3 increased significantly the conductivity and the leached solutes for both the varieties. Namulenga variety had the higher bulk density (0.81 g/ml) compared to HM21_7 (0.77 g/ml). The porosity varied significantly among the two varieties. A significant different (P<0.01) was observed in the colour of the beans after soaking. Beans soaked in distilled water were lighter due to greater pigment leaching. On the other hand, beans soaked in Na2CO3 solution for 16 h showed darker colour. Soaking in sodium carbonate solution prior to cooking significantly reduced the hardness of the beans compared to the unsoaked beans and beans soaked in distilled water. According to the characteristics studied, the Namulenga variety is a good option for both processing and domestic use. Keywords: Physical properties, Hardness, Bio-fortified beans

Impact of Storage Conditions on the Physical Properties and Cooking Characteristics of Two Bean Varieties Grown in Kenya

Common beans are highly nutritious and widely consumed in Kenya. Storage of common beans under adverse conditions of high temperature and high humidity renders them susceptible to the hard-to-cook (HTC) defect. This results in increased cooking time, fuel and water use which has a negative effect on acceptability and utilization of beans. The objective of this study was to determine effects of storage temperature and relative humidity on development of the HTC defect in Rose coco and Red Kidney bean varieties. Bean samples were obtained from Kenya Agricultural and Livestock Research Organization (K ALRO) - Thika. The beans were stored at varying temperature (25ºC, 35˚ C and 45˚ C) and relative humidity (RH=75% and 83%) combinations. Apart from beans stored at 25ºC/75%, samples from each treatment condition were sampled after every two months and analyzed for physical properties. Soaking pretreatments in deionized water, sodium carbonate and calcium chloride solutions were carried out to determine their effect on the cooking time. There was a significant increase in conductivity and leached solutes paralleled by decreasing hydration and swelling coefficients with increasing storage time under all the storage conditions. Characteristic dimensions and one hundred seed weight were not significantly different among the bean varieties under various storage conditions. Moisture uptake reduced by 19% for Rose coco and 23% for Red kidney under 35˚C/83%  storage whereas 45˚C/75% had a 29% reduction for Rose coco and 39% reduction for Red kidney over the 6 months storage period. Cooking time increased for all the bean varieties with increasing storage time, the most pronounced increase (100%) being observed at 45˚C and 75% RH. Across the 6 months storage period, Na2CO3 soaking pretreatment reduced the cooking time by 75% for Rose coco and 70% for Red kidney in comparison to beans cooked without prior soaking. Based on the results of this study, it was concluded that storage under high temperature and relative humidity conditions accelerated the development of the HTC defect in beans resulting in changes in physical and cooking properties. Keywords: hard-to-cook, storage, physical properties, cooking qualit

Seasonal Patterns of Herbage Accumulation Dynamics in Marandu Palisadegrass Subjected to Intensities of Continuous Stocking Management

It is relatively well reported in the literature that pastures can have similar forage net accumulation when managed with contrasting structures. However, we hypothesized that the patterns of forage accumulation dynamics of pastures managed at different canopy heights is dependent on environmental conditions. The experimental treatments were four canopy heights (10, 20, 30, and 40 cm), allocated to experimental units according to a randomized complete block design with four replicates and evaluated throughout four contrasting environmental seasons (Summer, Autumn, Winter-Early Spring, and Late Spring). Under favourable growing conditions greater forage accumulation was observed in pastures maintained taller; on the contrary, under more stressful conditions, net forage accumulation rate reduced as canopy height increased. Such patterns of responses were related to compensations between tiller population density and tissue flows during summer and late spring and the reduced capacity of taller canopies to compensate lower population with greater growth rates during autumn and winter-early spring. Pastures subjected to intensities of continuous stocking management change their patterns of forage growth as they transitioned from favourable to more abiotic stressful conditions suggesting that warm-season perennial grasses demand seasonal adjustments in grazing heights in order to maximize herbage production

Crescimento do capim-marandu submetido à estratégias de lotação intermitente

Grazing strategies alter sward leaf area patterns of growth, affecting herbage accumulation and utilisation. The objective of this experiment was to evaluate the growth of marandu palisadegrass (Brachiaria brizantha cv. Marandu) swards subjected to strategies of intermittent stocking. The experiment was carried out in Piracicaba, São Paulo, Brazil, from October/2004 to September/2005. Swards were grazed at 95 and 100% canopy light interception (LI) to post-grazing heights of 10 and 15 cm, following a 2 × 2 factorial arrangement with four replications in a randomised complete block design. The response variables evaluated were: crop growth rate, relative growth rate, net assimilation rate, leaf area ratio and leaf weight ratio. In early and late spring, the highest crop growth rate was recorded for treatment 95/15 (11.2 and 10.1 g m-2 day-1, respectively), along with high values of net assimilation rate (4.4 and 6.9 g m-2 day-1, respectively), leaf area ratio (0.0095 and 0.0103 m-2 g-1, respectively) and leaf weight ratio (0.56 and 0.56 g g-1, respectively). To compensate reductions in net assimilation rate plants made some morphological and physiological adjustments increasing leaf area and leaf weight ratio. Relative growth rate and net assimilation rate were 26 and 50% higher, respectively, on swards grazed at 95% than at 100% LI. In early spring treatments 100/10 and 95/15 resulted in the highest relative growth rate (0.086 and 0.059 g m-2 day-1, respectively). Treatment 95/15 resulted in the most favourable pattern of growth (crop growth rate, relative growth rate, net assimilation rate), particularly during the transition period between winter and spring.Estratégias de pastejo alteram a área foliar dos pastos, interferindo em seus padrões de crescimento e afetando o acúmulo e a utilização de forragem. O objetivo desse experimento foi avaliar o crescimento de pastos de capim-marandu (Brachiaria brizantha cv. Marandu) submetidos a estratégias de lotação intermitente. O experimento foi realizado em Piracicaba, SP, Brasil, de Outubro/2004 a Setembro/2005. Os pastejos foram realizados quando o dossel atingia 95 ou 100% de interceptação luminosa (IL) até as alturas pós-pastejo de 10 e 15 cm, seguindo um arranjo fatorial 2 × 2 com 4 repetições e um delineamento de blocos completos casualizados. Avaliaram-se a taxa de crescimento da cultura, a taxa de crescimento relativo, a taxa de assimilação líquida, a razão de área foliar e a razão de peso foliar. No início e final de primavera a maior taxa de crescimento da cultura foi registrada para o tratamento 95/15 (11.2 e 10.1 g m-2 dia-1, respectivamente), associada a altos valores de taxa de assimilação líquida (4.4 e 6.9 g m-2 dia-1, respectivamente), razão de área foliar (0.0095 e 0.0103 m-2 g-1, respectivamente) e razão de peso foliar (0.56 e 0.56 g g-1, respectivamente). Para compensar reduções em taxa de assimilação líquida a planta se ajustou morfológica e fisiologicamente aumentando a razão de área foliar e razão de peso foliar. A taxa de crescimento relativo e a taxa de assimilação líquida foram, respectivamente, 26% e 50% superiores nos pastos manejados com 95% relativamente àqueles com 100% de IL. No início de primavera os tratamentos 100/10 e 95/15 resultaram na maior taxa de crescimento relativo (0.086 e 0.059 g m-2 dia-1, respectivamente). O tratamento 95/15 resultou no padrão mais favorável de crescimento (taxas de crescimento da cultura, crescimento relativo e assimilatória líquida), particularmente durante o período de transição entre inverno e primavera.CNPqFAPES

Growth of marandu palisadegrass subjected to strategies of intermittent stocking

Grazing strategies alter sward leaf area patterns of growth, affecting herbage accumulation and utilisation. The objective of this experiment was to evaluate the growth of marandu palisadegrass (Brachiaria brizantha cv. Marandu) swards subjected to strategies of intermittent stocking. The experiment was carried out in Piracicaba, São Paulo, Brazil, from October/2004 to September/2005. Swards were grazed at 95 and 100% canopy light interception (LI) to post-grazing heights of 10 and 15 cm, following a 2 × 2 factorial arrangement with four replications in a randomised complete block design. The response variables evaluated were: crop growth rate, relative growth rate, net assimilation rate, leaf area ratio and leaf weight ratio. In early and late spring, the highest crop growth rate was recorded for treatment 95/15 (11.2 and 10.1 g m-2 day-1, respectively), along with high values of net assimilation rate (4.4 and 6.9 g m-2 day-1, respectively), leaf area ratio (0.0095 and 0.0103 m-2 g-1, respectively) and leaf weight ratio (0.56 and 0.56 g g-1, respectively). To compensate reductions in net assimilation rate plants made some morphological and physiological adjustments increasing leaf area and leaf weight ratio. Relative growth rate and net assimilation rate were 26 and 50% higher, respectively, on swards grazed at 95% than at 100% LI. In early spring treatments 100/10 and 95/15 resulted in the highest relative growth rate (0.086 and 0.059 g m-2 day-1, respectively). Treatment 95/15 resulted in the most favourable pattern of growth (crop growth rate, relative growth rate, net assimilation rate), particularly during the transition period between winter and spring.Estratégias de pastejo alteram a área foliar dos pastos, interferindo em seus padrões de crescimento e afetando o acúmulo e a utilização de forragem. O objetivo desse experimento foi avaliar o crescimento de pastos de capim-marandu (Brachiaria brizantha cv. Marandu) submetidos a estratégias de lotação intermitente. O experimento foi realizado em Piracicaba, SP, Brasil, de Outubro/2004 a Setembro/2005. Os pastejos foram realizados quando o dossel atingia 95 ou 100% de interceptação luminosa (IL) até as alturas pós-pastejo de 10 e 15 cm, seguindo um arranjo fatorial 2 × 2 com 4 repetições e um delineamento de blocos completos casualizados. Avaliaram-se a taxa de crescimento da cultura, a taxa de crescimento relativo, a taxa de assimilação líquida, a razão de área foliar e a razão de peso foliar. No início e final de primavera a maior taxa de crescimento da cultura foi registrada para o tratamento 95/15 (11.2 e 10.1 g m-2 dia-1, respectivamente), associada a altos valores de taxa de assimilação líquida (4.4 e 6.9 g m-2 dia-1, respectivamente), razão de área foliar (0.0095 e 0.0103 m-2 g-1, respectivamente) e razão de peso foliar (0.56 e 0.56 g g-1, respectivamente). Para compensar reduções em taxa de assimilação líquida a planta se ajustou morfológica e fisiologicamente aumentando a razão de área foliar e razão de peso foliar. A taxa de crescimento relativo e a taxa de assimilação líquida foram, respectivamente, 26% e 50% superiores nos pastos manejados com 95% relativamente àqueles com 100% de IL. No início de primavera os tratamentos 100/10 e 95/15 resultaram na maior taxa de crescimento relativo (0.086 e 0.059 g m-2 dia-1, respectivamente). O tratamento 95/15 resultou no padrão mais favorável de crescimento (taxas de crescimento da cultura, crescimento relativo e assimilatória líquida), particularmente durante o período de transição entre inverno e primavera

Components of the leaf area index of marandu palisadegrass swards subjected to strategies of intermittent stocking

Leaf area index is the main sward characteristic related to the processes of light interception and competition in plant communities. The objective of this experiment was to quantify and evaluate the composition of the leaf area on tillers of marandu palisadegrass (Brachiaria brizantha cv. Marandu) subjected to strategies of intermittent stocking. The experiment was carried out in Piracicaba, state of São Paulo, Brazil, from October/2004 to December/2005. Swards were grazed at 95 and 100% canopy light interception (LI) to post-grazing heights of 10 and 15 cm, following a 2 ' 2 factorial arrangement with four replications in a randomised complete block design. Estimates were made of sward leaf area index, site filling, specific leaf area and the dimensionless ratio between tiller leaf area and volume (R), as well as the relative contribution of basal and aerial tillers to these variables. In early spring, values of leaf area index and specific leaf area were low when compared to the other seasons, and swards grazed at 95% LI presented higher site filling and specific leaf area than those grazed at 100% LI. This resulted in higher tillering activity and increase in leaf area index in late spring, indicating quick recovery and early return of swards grazed at 95% LI to growing conditions. Aerial tillers corresponded to an important morphological adaptation of marandu palisadegrass to increase its competitive ability. Treatment 100/10 resulted in the highest and 95/15 in the lowest R values throughout the experiment, suggesting an allometric pattern of growth of tillers during regrowth in order to compensate low tiller population and optimise the leaf area index. Grazing management practices can benefit from this knowledge by promoting ideal sward conditions to maximise and accelerate growth.O índice de área foliar é a principal característica do dossel relacionada com os processos de interceptação e competição por luz em comunidades de plantas. O objetivo deste experimento foi quantificar e avaliar a composição da área foliar dos perfilhos em pastos de capim-marandu (Brachiaria brizantha cv. Marandu) submetidos a estratégias de lotação intermitente. O experimento foi conduzido em Piracicaba, SP, Brasil, de outubro/2004 a dezembro/2005. Os pastejos foram realizados quando o dossel atingia 95 ou 100% de interceptação luminosa (IL) até as alturas pós-pastejo de 10 e 15 cm, seguindo um arranjo fatorial 2 ' 2 com 4 repetições e um delineamento de blocos completos casualizados. Foram estimados o índice de área foliar, site filling, área foliar específica e a relação adimensional entre área foliar e volume dos perfilhos (R), além da participação relativa de perfilhos basais e aéreos na composição dessas variáveis. No início de primavera os valores de índice de área foliar e área foliar específica foram baixos, e pastos manejados com 95% de IL apresentaram maior site filling e área foliar específica que pastos manejados com 100% de IL. Isso resultou em maior perfilhamento e aumento no índice de área foliar no final de primavera, indicando rápida recuperação e retorno precoce dos pastos manejados com 95% de IL a condições de crescimento. Os perfilhos aéreos corresponderam a uma importante adaptação morfológica do capim-marandu para aumentar sua habilidade competitiva. O tratamento 100/10 resultou nos maiores e o 95/15 nos menores valores de R, sugerindo um padrão alométrico de crescimento dos perfilhos durante a rebrotação para compensar baixa densidade populacional e otimizar o índice de área foliar. Práticas de manejo do pastejo podem se beneficiar desse conhecimento propiciando condições ideais de dossel para maximização e aceleração do crescimento

Effect of Differences in Month and Location of Measurement in Estimating Prevalence and Trend of Wasting and Stunting in India in 2005-2006 and 2015-2016

Background: Child undernutrition in India remains widespread. Data from the National Family Health Survey 3 and 4 (NFHS-3 and NFHS-4) suggest that wasting prevalence has increased while stunting prevalence has declined. Objective: The objectives of this study were to do the following: ) describe wasting and stunting by month of measurement in India in children surveys, and ) test whether differences in the timing of anthropometric data collection and in states between survey years introduced bias in the comparison of estimates of wasting and stunting between NFHS-3 and NFHS-4. Methods: Data on wasting and stunting for 42,608 and 232,744 children aged \u3e5 y in the NFHS-3 and NFHS-4 survey rounds were analyzed. Differences in the prevalence of wasting and stunting by month of year and by state of residence were examined descriptively. Regression analyses were conducted to test the sensitivity of the estimate of differences in wasting and stunting prevalence across survey years to both state differences and seasonality. Results: Examination of the patterns of wasting and stunting by month of measurement and by state across survey years reveal marked variability. When both state and month were adjusted, regardless of the method used to account for sample size, there was a small negative difference from 2005-2006 to 2015-2016 in the prevalence of wasting (-0.8 ± 0.6 percentage points; = 0.2) and a negative difference in stunting prevalence (-8.3 ± 0.7 percentage points; \u3c 0.001), indicating a small bias for wasting but not for stunting in unadjusted analyses. Conclusions: State and seasonal differences may have introduced bias to the estimated difference in prevalence of wasting between the survey years but did not do so for stunting. Future data collection should be designed to maximize consistency in coverage of both time and place

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London