A Combined Approach For Private Indexing Mechanism

Private indexing is a set of approaches for analyzing research data that are similar or resemble similar ones. This is used in the database to keep track of the keys and their values. The main subject of this research is private indexing in record linkage to secure the data. Because unique personal identification numbers or social security numbers are not accessible in most countries or databases, data linkage is limited to attributes such as date of birth and names to distinguish between the number of records and the real-life entities they represent. For security reasons, the encryption of these identifiers is required. Privacy-preserving record linkage, frequently used to link private data within several databases from different companies, prevents sensitive information from being exposed to other companies. This research used a combined method to evaluate the data, using classic and new indexing methods. A combined approach is more secure than typical standard indexing in terms of privacy. Multibit tree indexing, which groups comparable data in many ways, creates a scalable tree-like structure that is both space and time flexible, as it avoids the need for redundant block structures. Because the record pair numbers to compare are the Cartesian product of both the file record numbers, the work required grows with the number of records to compare in the files. The evaluation findings of this research showed that combined method is scalable in terms of the number of databases to be linked, the database size, and the time required

AYURVEDIC GARBHA VIKAS KRAM IN CONTEXT TO EMBRYOGENESIS AND FOETAL DEVELOPMENT

Sushrut samhita is one of those Ayurvedic classic which deals with human anatomy and physiology. It gives precise description of Garbha vruddhi and vikas kram though there were no modern imaging techniques like sonography. Different Granthas state various views regarding Garbha utpatti and Garbha vriddhi. Acharyas have also mentioned Garbhini lakshana which are helpful in diagnosis of early pregnancy. Charak has mentioned signs observed in Garbhini with progression of pregnancy. Whereas modern obstetrics is one of the most cardinal branch which concerned with human reproduction. This science provide each minute detail regarding foetal growth and development. Tremendous research has been done in the field of modern obstetrics.Foetal deaths and neonatal mortality is always an issue of concern. One can get relevant references of these issues in Samhitas. Acharyas has mentioned topic of Garbha in concise form. Extensive study and discussion is essential to understand ancient concept of Garbha vikas. Review of Ayurvedic text along with modern text will help to obtain knowledge regarding this. This study is an attempt to elaborate and compare both ancient and modern concept of embryogenesis. There might be some variation when we consider both perspectives. But it is really interesting to find similarities beside a huge gap of time

TGF-β Signaling Initiated in Dendritic Cells Instructs Suppressive Effects on Th17 Differentiation at the Site of Neuroinflammation

While the role of Transforming Growth Factor β (TGF-β) as an intrinsic pathway has been well established in driving de novo differentiation of Th17 cells, no study has directly assessed the capacity of TGF-β signaling initiated within dendritic cells (DCs) to regulate Th17 differentiation. The central finding of this study is the demonstration that Th17 cell fate during autoimmune inflammation is shaped by TGF-β extrinsic pathway via DCs. First, we provide evidence that TGF-β limits at the site of inflammation the differentiation of highly mature DCs as a means of restricting Th17 cell differentiation and controlling autoimmunity. Second, we demonstrate that TGF-β controls DC differentiation in the inflammatory site but not in the priming site. Third, we show that TGF-β controls DC numbers at a precursor level but not at a mature stage. While it is undisputable that TGF-β intrinsic pathway drives Th17 differentiation, our data provide the first evidence that TGF-β can restrict Th17 differentiation via DC suppression but such a control occurs in the site of inflammation, not at the site of priming. Such a demarcation of the role of TGF-β in DC lineage is unprecedented and holds serious implications vis-à-vis future DC-based therapeutic targets

Formulation and evaluation of prazosin hydrochloride loaded solid lipid nanoparticles

Solid Lipid Nanoparticles (SLN) are rapidly developing field of nanotechnology with several potential application in drug delivery and research. Drugs having low aqueous solubility not only give low oral bioavailability but provide high inter-and intra subject variability. The purpose of the present study was to investigate the bioavailability enhancement of Prazosin Hydrochloride drug by formulating solid lipid nanoparticle. Prazosin Hydrochloride Drug is an antihypertensive drug with limited bioavailability so that solid lipid nanoparticle (SLN) is one of the approaches to improve bioavailability. SLN were prepared using glyceryl monostearate by hot homogenization followed by Solvent emulsification-ultrasonication. Prazosin Hydrochloride loaded SLN were characterized and optimized by parameters like particle size, zeta potential, XRD, DSC. Proposing Hydrochloride loaded SLN having the particle size 263.8±1.88 and entrapment efficiency 89.29±0.65% shows better bioavailability and optimum stability in studies. The SLN studies prepared using glyceryl mono stearate   as a lipid and Polaxamer 407 as a polymer leads to improve bioavailability of the drug. Keywords: Prazosin Hydrochloride, Solid Lipid Nanoparticles, Entrapment efficiency, DS

The role of plant growth promoting bacteria on arsenic removal: a review of existing perspectives

Phytobial remediation is an innovative tool that uses plants and microbes to mitigate Arsenic (As) contamination of the environment. Recently, plant growth-promoting bacteria (PGPB) that assists phytoremediation has been highly touted for both improving plant metal tolerance and promoting plant growth while achieving the goal of large-scale removal of As. This review focuses on the PGPB characteristics influencing plants and the mechanisms in which they function to overcome/lessen As-induced adversities. Several recent examples of mechanisms responsible for increasing the availability of As to plants and coping with As stresses facilitated by PGPB will be reviewed. Although drawbacks to phytoremediation have been reported, encouraging results have been developed with regular monitoring. Introducing PGPB-assisted phytoremediation of As in a field requires an assessment of the environmental effects of PGPB, especially with respect to the impacts on indigenous bacteria

Performance of the CMS High Granularity Calorimeter prototype to charged pion beams of 20−-300 GeV/c

The upgrade of the CMS experiment for the high luminosity operation of the LHC comprises the replacement of the current endcap calorimeter by a high granularity sampling calorimeter (HGCAL). The electromagnetic section of the HGCAL is based on silicon sensors interspersed between lead and copper (or copper tungsten) absorbers. The hadronic section uses layers of stainless steel as an absorbing medium and silicon sensors as an active medium in the regions of high radiation exposure, and scintillator tiles directly readout by silicon photomultipliers in the remaining regions. As part of the development of the detector and its readout electronic components, a section of a silicon-based HGCAL prototype detector along with a section of the CALICE AHCAL prototype was exposed to muons, electrons and charged pions in beam test experiments at the H2 beamline at the CERN SPS in October 2018. The AHCAL uses the same technology as foreseen for the HGCAL but with much finer longitudinal segmentation. The performance of the calorimeters in terms of energy response and resolution, longitudinal and transverse shower profiles is studied using negatively charged pions, and is compared to GEANT4 predictions. This is the first report summarizing results of hadronic showers measured by the HGCAL prototype using beam test data.Comment: To be submitted to JINS

In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in Escherichia coli (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their Components

Rapid assessment of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-based genome editing (GE) tools and their components is a critical aspect for successful GE applications in different organisms. In many bacteria, double-strand breaks (DSBs) generated by CRISPR/Cas tool generally cause cell death due to the lack of an efficient nonhomologous end-joining pathway and restricts its use. CRISPR-based DSB-free base editors (BEs) have been applied for precise nucleotide (nt) editing in bacteria, which does not need to make DSBs. However, optimization of newer BE tools in bacteria is challenging owing to the toxic effects of BE reagents expressed using strong promoters. Improved variants of two main BEs, cytidine base editor (CBE) and adenine base editor (ABE), capable of converting C to T and A to G, respectively, have been recently developed but yet to be tested for editing characteristics in bacteria. Here, we report a platform for in vivo rapid investigation of CRISPR-BE components in Escherichia coli (IRI-CCE) comprising a combination of promoters and terminators enabling the expression of nCas9-based BE and sgRNA to nontoxic levels, eventually leading to successful base editing. We demonstrate the use of IRI-CCE to characterize different variants of CBEs (PmCDA1, evoCDA1, APOBEC3A) and ABEs (ABE8e, ABE9e) for bacteria, exhibiting that each independent BE has its specific editing pattern for a given target site depending on protospacer length. In summary, CRISPR-BE components expressed without lethal effects on cell survival in the IRI-CCE allow an analysis of various BE tools, including cloned biopart modules and sgRNAs

Quality assurance of locally produced HGCAL baseplates

To cope with severe radiation dosage and increased event pileup in a high luminosity environment, the existing endcap calorimeters of the CMS experiment will be replaced by a high granularity calorimeter (HGCAL). To make the most of the increased granularity, a precise placement of detector modules into the sampling planes is of utmost importance. This requires the module components' physical dimensions and other features to be within strict bounds. We have developed a suite of programs for an optical coordinate measuring machine, used to perform various measurements on HGCAL baseplates, and have subsequently extracted quality assurance results from the recorded data. Our key objective is to verify the baseplates produced in India for their quality, such as flatness, thickness, and accuracy in fiducial placement, and to provide feedback to the manufacturers for better quality control