Developing Handbook: South Central League Standard Operating Procedures

The purpose of this project was to explore existing standard operating procedures of athletic leagues from around the State of Washington and to develop a Standard Operating Procedures Manual for use by the South Central A League of District IV of the Washington Interscholastic Activities Association. The results showed that although all leagues are required to have such a manual, the governing body of high school athletics, Washington Interscholastic Activities Association (W.I.A.A.), recommended that only five league handbooks be used as models toward the development of this project

Tratamiento del cuerpo y control social entre los mayas itzaes, siglos XVII-XVIII

In many societies the human body is considered as a cosmological model and therefore of society. In the case of the Maya Itza we know that the human body is a reference to their territorial, social and religious organization. In this paper we analyze the importance of the human body as a symbolic model, focusing on aspects of Itza religion and rituals, related to the transformation and modification of the human body. We will analyze how Itza society controlled a population with the ability of transfiguration.En diversas sociedades el cuerpo humano se ha concebido como un modelo cosmológico y por lo tanto de la sociedad. En el caso de los mayas itzaes sabemos que el cuerpo humano es un referente para la organización territorial, social y religiosa. En este trabajo se analiza la importancia del cuerpo humano como modelo simbólico, centrándonos en aspectos de la religión y rituales itzaes relacionados con la transformación y modificación del cuerpo humano en el período colonial. Se examina el control que ejerció la sociedad itzá con respecto a una población con la capacidad de transfiguración

Zona incerta neurons projecting to the ventral tegmental area promote action initiation towards feeding

Key pointsThe zona incerta (ZI) and ventral tegmental area (VTA) are brain areas that are both implicated in feeding behaviour. The ZI projects to the VTA, although it has not yet been investigated whether this projection regulates feeding.We experimentally (in)activated the ZI to VTA projection by using dual viral vector technology, and studied the effects on feeding microstructure, the willingness to work for food, general activity and body temperature.Activity of the ZI to VTA projection promotes feeding by facilitating action initiation towards food, as reflected in meal frequency and the willingness to work for food reward, without affecting general activity or directly modulating body temperature.We show for the first time that activity of the ZI to VTA projection promotes feeding, which improves the understanding of the neurobiology of feeding behaviour and body weight regulation.Both the zona incerta (ZI) and the ventral tegmental area (VTA) have been implicated in feeding behaviour. The ZI provides prominent input to the VTA, although it has not yet been investigated whether this projection regulates feeding. Therefore, we investigated the role of ZI to VTA projection neurons in the regulation of several aspects of feeding behaviour. We determined the effects of (in)activation of ZI to VTA projection neurons on feeding microstructure, food-motivated behaviour under a progressive ratio schedule of reinforcement, locomotor activity and core body temperature. To activate or inactivate ZI neurons projecting to the VTA, we used a combination of canine adenovirus-2 in the VTA, as well as Cre-dependent designer receptors exclusively activated by designer drugs (DREADD) or tetanus toxin (TetTox) light chain in the ZI. TetTox-mediated inactivation of ZI to VTA projection neurons reduced food-motivated behaviour and feeding by reducing meal frequency. Conversely, DREADD-mediated chemogenetic activation of ZI to VTA projection neurons promoted food-motivated behaviour and feeding. (In)activation of ZI to VTA projection neurons did not affect locomotor activity or directly regulate core body temperature. Taken together, ZI neurons projecting to the VTA exert bidirectional control overfeeding behaviour. More specifically, activity of ZI to VTA projection neurons facilitate action initiation towards feeding, as reflected in both food-motivated behaviour and meal initiation, without affecting general activity

Akhet Khufu: Archaeo-astronomical Hints at a Common Project of the Two Main Pyramids of Giza, Egypt

Abstract. The architectural complexes composed by the two main pyramids of Giza together with their temples are investigated from an interdisciplinary point of view, taking into account their astronomical alignments as well as their relationships with the visible landscape. Combining already known facts together with new clues, the work strongly supports the idea that the two complexes were conceived as parts of a common project

ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complex

DNA double-strand breaks (DSBs) threaten genome stability in all kingdoms of life and are linked to cancerogenic chromosome aberrations in humans. The Mre11:Rad50 (MR) complex is an evolutionarily conserved complex of two Rad50 ATPases and a dimer of the Mre11 nuclease that senses and processes DSBs and tethers DNA for repair. ATP binding and hydrolysis by Rad50 is functionally coupled to DNA-binding and tethering, but also regulates Mre11's nuclease in processing DNA ends. To understand how ATP controls the interaction between Mre11 and Rad50, we determined the crystal structure of Thermotoga maritima (Tm) MR trapped in an ATP/ADP state. ATP binding to Rad50 induces a large structural change from an open form with accessible Mre11 nuclease sites into a closed form. Remarkably, the NBD dimer binds in the Mre11 DNA-binding cleft blocking Mre11's dsDNA-binding sites. An accompanying large swivel of the Rad50 coiled coil domains appears to prepare the coiled coils for DNA tethering. DNA-binding studies show that within the complex, Rad50 likely forms a dsDNA-binding site in response to ATP, while the Mre11 nuclease module retains a ssDNA-binding site. Our results suggest a possible mechanism for ATP-dependent DNA tethering and DSB processing by MR