VARIAÇÃO DOS CONSTITUINTES BIOQUÍMICOS SANGUÍNEOS DE TARTARUGAS-DA-AMAZÔNIA (Podocnemis expansa, Schweigger 1812) (TESTUDINATA) MANTIDAS EM CRIATÓRIO COMERCIAL

The aim of the present research was to evaluate the biochemistry of blood constituents from the South American River Turtle Podocnemis expansa, Schweigger 1912. For this experiment, 46 animals from Moenda do Lago Farm, a commercial farm from São José dos Bandeirantes, Goiás State, Brazil, were used, the blood being collected from the vertebral sinus, trsnferred to vacuntainer tubes without anticoagulant, spun down, the serum collected in eppendorfs and storaged at 20C and the respective analysis carried out by colorimetric techniques and fast glucose sensor Precision Plus MedsenseÅ at the College of Veterinary Medicine Latobatories at Federal University of Uberlandia, Minas Gerais State, Brazil. The mean values found were as follows: Total proteins, 4.42 ± 0.55 g/dL, albumin, 2.50 ±0.2 g/dL, globulins, 1.92 ± 0.35 mg/dL, albumin/globulin ratio (A/G) 1.55 ±1.46, glucose, 122.9 ± 35.2 mg/dL, urea, 61.13 ± 28.35 mg/dL, creatinine, 0.098 ± 0.021 mg/dL, uric acid, 2.08 ± 0.53 mg/dL, cholesterol, 106.93 ± 21.03 mg/dL, total calcium,7.08 ± 1.04 mg/dL, ionic calcium, 4.41 ± 0.60 mg/dL, phosphorus, 5.21 ± 1.22 mg/dL, calcium/phosphorus ratio, 1.58 mg/dL ± 0.66, iron, 390.35 ± 116,65 µg/dL, magnesium, 1.59 ± 0.14, chlorides, 86.40 ± 7.63 mEq/ L, aspartate aminotransferase (AST) 194.56 ± 154.27 U/L, alanineaminotransferase (ALT) 4.04 ± 3.00 U/L, alkaline phosphatase, 131.13 ± 59.96 U/L, ¡- glutamiltransferase (GGT) 0.60 ± 0.84 U/L and tryglicerides, 127.65 ± 100.86 mg/dL. In conclusion, mean values of blood constituents of Podocnemis expansa did not show great differences from other chelonian species. The albumin/globulin ratio, and uric acid and glucose values were inversely related to body weight. Thus, the results of this research bring data on South America River Turtle blood biochemistry raised in captivity, although new research studies will be necessary to establish the species reference values.O objetivo desta pesquisa foi avaliar as variações dos constituintes bioquímicos sanguíneos da tartaruga Podocnemis expansa de um criatório comercial. Para isto, foram colhidas, por venipuntura do seio vertebral caudal, amostras de sangue de 46 animais mantidos em tanques na Fazenda Moenda do Lago, distrito de São José dos Bandeirantes, município de Nova Crixás GO. Após colhidas, as amostras de sangue foram transferidas rapidamente para frascos vacutainer® sem anticoagulante e levados à centrífuga para obtenção do soro, logo após transferidos para eppendorf e armazenados a 200C. O material foi transportado para o laboratório da Faculdade de Medicina Veterinária (FAMEV) da Universidade Federal de Uberlândia (UFU) para o seu processamento. As análises foram processadas colorimetricamente e em sensor rápido de glicose (Precision Plus Medsense®), os valores médios foram: proteínas totais 4,42±0,55 g/ dL, albumina 2,50±0,2 g/dL, globulinas 1,92±0,35 g/dL, relação albumina/globulinas (A/G) 1,55±1,46, glicose 122,9±35,2 mg/dL, uréia 61,13±28,35 mg/dL, creatinina 0,098±0,021 mg/dL, ácido úrico 2,08±0,53 mg/dL, cálcio total 7,08±1,04 mg/dL, cálcio ionizado 4,41±0,60 mg/ dL, colesterol 106,93±21,03 mg/dL, fósforo 5,21±1,22 mg/dL, relação cálcio/fósforo (Ca++/P) 1,58±0,66, ferro 390,35±116,65 ì g/dL, magnésio 1,59±0,14 mg/dL, cloretos 86,40±7,63 mEq/L, aspartato aminotransferase (AST) 194,56±154,27 U/L, alanino aminotransferase (ALT) 4,04±3,00 U/L, fosfatase alcalina 131,13±59,96 U/L, gama glutamiltransferase (GGT) 0,60±0,84 U/L e triglicérides 127,65±100,86 mg/dL. Conclui-se que os valores apresentam grandes diferenças dos valores de outras espécies de quelônios e que a relação albumina:globulina, as concentrações plasmáticas de ácido úrico e glicose são inversamente proporcionais às massas corporais dos animais. A espécie estudada apresenta escassos valores de bioquímica sanguínea na literatura, desta maneira os dados que foram apresentados auxiliam no estabelecimento de um padrão de bioquímica sanguínea para Podocnemis expansa criadas em cativeiro, mas também pode ser base para novos estudos

Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV

A search for a Higgs boson decaying into two photons is described. The analysis is performed using a dataset recorded by the CMS experiment at the LHC from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross section of the standard model Higgs boson decaying to two photons. The expected exclusion limit at 95% confidence level is between 1.4 and 2.4 times the standard model cross section in the mass range between 110 and 150 GeV. The analysis of the data excludes, at 95% confidence level, the standard model Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The largest excess of events above the expected standard model background is observed for a Higgs boson mass hypothesis of 124 GeV with a local significance of 3.1 sigma. The global significance of observing an excess with a local significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is estimated to be 1.8 sigma. More data are required to ascertain the origin of this excess.Comment: Submitted to Physics Letters

Measurement of isolated photon production in pp and PbPb collisions at sqrt(sNN) = 2.76 TeV

Isolated photon production is measured in proton-proton and lead-lead collisions at nucleon-nucleon centre-of-mass energies of 2.76 TeV in the pseudorapidity range |eta|<1.44 and transverse energies ET between 20 and 80 GeV with the CMS detector at the LHC. The measured ET spectra are found to be in good agreement with next-to-leading-order perturbative QCD predictions. The ratio of PbPb to pp isolated photon ET-differential yields, scaled by the number of incoherent nucleon-nucleon collisions, is consistent with unity for all PbPb reaction centralities.Comment: Submitted to Physics Letters