Tumor de Wilms em adulto: relato de caso

O tumor de Wilms é a neoplasia renal de maior incidência na primeira infância e raramente acomete adultos. A incidência neste grupo situa-se em torno de 1% do número total dos casos, sendo o prognóstico bem pior. Neste trabalho é relatado um caso novo em paciente do sexo masculino, com 52 anos de idade, apresentando dor abdominal intensa associada à perda ponderal. A ultra-sonografia abdominal revelou lesão expansiva complexa, de contornos indefinidos em flanco esquerdo. A tomografia computadorizada de abdome demonstrou lesão sólida no pólo antero-superior do rim esquerdo invadindo os espaços peri-renal, para-renal e musculatura para-vertebral. Foi realizada nefrectomia total e posterior exame anatomo-patológico da peça cirúrgica que diagnosticou nefroblastoma (tumor de Wilms) em estádio II, sem anaplasia. O paciente recebeu radioterapia no leito tumoral. Diagnosticouse metástase pulmonar e hepática após o término da mesma, quando se iniciou a quimioterapia com adriamicina, actinomicina e vincristina. O tumor de Wilms em adulto apresenta geralmente um prognóstico pior, necessitando de uma terapêutica mais agressiva e de acompanhamento prolongado

The validity and reliability of the Portuguese versions of three tools used to diagnose delirium in critically ill patients

OBJECTIVES: The objectives of this study are to compare the sensitivity and specificity of three diagnostic tools for delirium (the Intensive Care Delirium Screening Checklist, the Confusion Assessment Method for Intensive Care Units and the Confusion Assessment Method for Intensive Care Units Flowsheet) in a mixed population of critically ill patients, and to validate the Brazilian Portuguese Confusion Assessment Method for Intensive Care Units. METHODS: The study was conducted in four intensive care units in Brazil. Patients were screened for delirium by a psychiatrist or neurologist using the Diagnostic and Statistical Manual of Mental Disorders. Patients were subsequently screened by an intensivist using Portuguese translations of the three tools. RESULTS: One hundred and nineteen patients were evaluated and 38.6% were diagnosed with delirium by the reference rater. The Confusion Assessment Method for Intensive Care Units had a sensitivity of 72.5% and a specificity of 96.2%; the Confusion Assessment Method for Intensive Care Units Flowsheet had a sensitivity of 72.5% and a specificity of 96.2%; the Intensive Care Delirium Screening Checklist had a sensitivity of 96.0% and a specificity of 72.4%. There was strong agreement between the Confusion Assessment Method for Intensive Care Units and the Confusion Assessment Method for Intensive Care Units Flowsheet (kappa coefficient = 0.96) CONCLUSION: All three instruments are effective diagnostic tools in critically ill intensive care unit patients. In addition, the Brazilian Portuguese version of the Confusion Assessment Method for Intensive Care Units is a valid and reliable instrument for the assessment of delirium among critically ill patients

Effect edible antimicrobial coating in the white blush and anaerobic psychrotrophs baby carrots

[POR] Objetivou-se avaliar a qualidade visual e microbiológica de minicenouras submetidas a diferentes concentrações de amido de milho em suspensão utilizado para revestimento comestível. Cenouras cv. Esplanada colhidas aos 90 dias, minimamente processadas na forma de minicenouras, foram imersas em suspensão contendo 2, 3 ou 4% de amido de milho; 1,5 % de quitosana, 2 % de glicerol e 0,4 % de ácido acético glacial e mantidas sob ventilação. Minicenouras não imersas em suspensão, serviram de controle. Em seguida, as minicenouras foram embaladas em bandejas de polipropileno envoltas em filme de polivinilcloreto e mantidas a 5±1ºC, por 12 ou 15 dias. Minicenouras sem revestimento, apresentaram intenso aumento no índice de esbranquecimento, e no quarto dia, apresentaram entre 21 a 60 % de sua superfície esbranquecida. Independente da concentração de amido na suspensão, o índice de esbranquecimento não alterou. As minicenouras tratadas em suspensão contendo 4 % de amido, não apresentaram sintomas visuais de esbranquecimento durante todo o armazenamento. As minicenouras tratadas em suspensão contendo 2 ou 3% de amido, apresentaram até 20% da superfície esbranquecida, nas extremidades, sem comprometer sua qualidade visual. O tempo para secagem do revestimento foi de proximadamente 2,5; 3,5 e 5h para minicenoura tratadas em suspensão com 2, 3 e 4 %, respectivamente, dificultando o uso de suspensão mais concentrada. A velocidade de perda de massa fresca apresentou a mesma tendência para todos os tratamentos. O revestimento utilizado contendo quitosana, inibiu em 1,2 ciclos logs o crescimento de psicotróficos anaeróbicos após 15 dias, em relação ao controle. A suspensão contendo 2 ou 3 % de amido de milho, proporcionou manutenção da coloração característica de minicenoura e inibiu parcialmente o crescimento de psicotróficos anaeróbicos. Apoio financeiro: CAPES, CNPq e FAPEMIG. [ENG] This work aimed to develop an edible antimicrobial coating base on a starch- chitosan matrix to evaluate is effect on baby carrot by means of visual quality and anaerobic Psychrotrophs analyses. Carrots of, Esplanada cultivar were harvested at 90 days after planting, were minimally processed in form of baby carrots and immersed in the suspensión coating based on 2; 3 or 4 % maiz starch (w/v) + 2 % glycerol (v/v) + chitosan (1,5 %, w/v) and 0,4 % glacial acetic acid (v/v). All the samples were placed in extended polystyrene trays, wrapped in polyvinylchloride film and stored at 5±1 ºC for 12 or 15 days. Baby carrot no immersed, presented intense increase whiteness index, and 21 – 60 % at days four whitness surface. Independent of the starch concentration in the suspension, the whiteness index did not modify. The Baby carrot treated in suspension contend 4 % of starch, had not presented visual symptoms of whiteness during the storage. The baby carrots treated in suspension contend 2 or 3% of starch, had presented up to 20 % of the whiteness surface, in the extremities, without compromising its visual quality. The time for drying of the coatings was of approximately 2,5; 3,5 and 5h for baby carrot treated in suspension with 2, 3 and 4 %, respectively, making it difficult the use of more concentred suspension. The speed of mass loss presented the same trend for all the treatments. The use chitosan in the coating, inhibited in 1,2 cycles logs the growth of anaerobic psychrotrophs after 15 days, in relation to the control. The suspensión contend 2 or 3 % of maize starch, provided maintenance of the characteristic coloration of baby carrot and inhibited partially the anaerobic psychrotrophs growth.Os autores agradecem ao prof. Ricardo Henrique Silva Santos e estudantes pelo fornecimento da matéria-prima. À CAPES, CNPq e FAPEMIG pelo apoio financeiro

Microbial community profile and water quality in a protected area of the caatinga biome

The Caatinga is a semi-arid biome in northeast Brazil. The Paraguaçú River is located in the Caatinga biome, and part of its course is protected by the National Park of Chapada Diamantina (PNCD). In this study we evaluated the effect of PNCD protection on the water quality and microbial community diversity of this river by analyzing water samples obtained from points located inside and outside the PNCD in both wet and dry seasons. Results of water quality analysis showed higher levels of silicate, ammonia, particulate organic carbon, and nitrite in samples from the unprotected area compared with those from protected areas. Pyrosequencing of the 16S rRNA genes revealed that Burkholderiales was abundant in samples from all three sites during both seasons and was represented primarily by the genus Polynucleobacter and members of the Comamonadaceae family (e.g., genus Limnohabitans). During the dry season, the unprotected area showed a higher abundance of Flavobacterium sp. and Arthrobacter sp., which are frequently associated with the presence and/or degradation of arsenic and pesticide compounds. In addition, genes that appear to be related to agricultural impacts on the environment, as well as those involved in arsenic and cadmium resistance, copper homeostasis, and propanediol utilization, were detected in the unprotected areas by metagenomic sequencing. Although PNCD protection improves water quality, agricultural activities around the park may affect water quality within the park and may account for the presence of bacteria capable of pesticide degradation and assimilation, evidencing possible anthropogenic impacts on the Caatinga

APICULTURA E RESPONSABILIDADE SOCIAL: DESAFIOS DA PRODUÇÃO E DIFICULDADES EM ADOTAR PRÁTICAS SOCIAL E AMBIENTALMENTE RESPONSÁVEIS

RESUMOO artigo objetiva identificar a percepção e as dificuldades que os produtores têm em adotar práticas mais responsáveis social e ambientalmente. Utilizou-se de método qualitativo, com entrevista semiestruturada com cinco das seis pessoas que participam da Associação de Apicultores. Nesse sentido, a pesquisa utilizou a análise categorial temática, com o desmembramento em categorias conforme os temas que emergem do texto identificando o que eles têm em comum, dividida em quatro categorias temáticas: 3.1 - Perfil dos entrevistados; 3.2 - Determinação da criação de abelhas e da produção do mel; 3.3 - Conhecimento da atividade e importância da associação; 3.4 - Comercialização e responsabilidade social e ambiental. A responsabilidade social em relação ao mel foi evidenciada como um diferencial no produto concentrado nas preocupações com o meio ambiente, influenciando as tomadas de decisões dos apicultores em seus negócios, bem como no comprometimento para a melhoria da qualidade de vida e bem-estar social. Os resultados da pesquisa também demonstraram que a apicultura é uma atividade lucrativa. No entanto, se percebeu que, em decorrência dos associados não estarem bem organizados, têm dificuldades em comercializar seus produtos

Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & Nemésio 2007; Donegan 2008, 2009; Nemésio 2009a–b; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on 18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based researchers who signed it in the short time span from 20 September to 6 October 2016

Educomunicação e suas áreas de intervenção: Novos paradigmas para o diálogo intercultural

oai:omp.abpeducom.org.br:publicationFormat/1O material aqui divulgado representa, em essência, a contribuição do VII Encontro Brasileiro de Educomunicação ao V Global MIL Week, da UNESCO, ocorrido na ECA/USP, entre 3 e 5 de novembro de 2016. Estamos diante de um conjunto de 104 papers executivos, com uma média de entre 7 e 10 páginas, cada um. Com este rico e abundante material, chegamos ao sétimo e-book publicado pela ABPEducom, em seus seis primeiros anos de existência. A especificidade desta obra é a de trazer as “Áreas de Intervenção” do campo da Educomunicação, colocando-as a serviço de uma meta essencial ao agir educomunicativo: o diálogo intercultural, trabalhado na linha do tema geral do evento internacional: Media and Information Literacy: New Paradigms for Intercultural Dialogue

Interaction between saliva’s adenosine and tick parasitism: effects on feeding and reproduction

Abstract\ud \ud Background\ud It has recently been demonstrated that saliva from Rhipicephalus sanguineus ticks contains adenosine (ADO) and prostaglandin E2 (PGE2), two non-protein molecules that have significant immunomodulatory properties. These molecules can inhibit cytokine production by dendritic cells (DCs), while also reducing the expression of CD40 in these cells. However, more studies are needed for a better understanding of their participation in the feeding of ticks in vivo. This work, therefore, evaluated the importance of ADO during tick infestations. Mice were infested with adult ticks (3 couples/mouse), and their skin was collected at the tick-infested site (3rd and 7th day), and mRNA for receptors of ADO was quantified by real-time PCR.\ud \ud \ud Results\ud Tick infestation increased by four and two times the expression of the A2b and A3v1 receptors on day 3, respectively, while expression of other ADO receptors was unaltered. In addition, we treated mice (n = 10/group) daily with 8-(p-Sulfophenyl)theophylline, 8-pSPT, 20 mg/kg, i.p.), a non-selective antagonist of ADO receptors, and evaluated the performance of ticks during infestations. Female ticks fed on 8-pSPT-treated mice presented a reduction in their engorgement, weight and hatching rates of egg masses, and survival times of larvae compared to the same parameters presented by ticks in the control group. To investigate if these 8-pSPT-treated mice presented altered immune responses, we performed three tick infestations and collected their lymph node cells to determine the percentages and activation state of DCs and cytokine production by lymphocytes by flow cytometry (Cytometric Bead Array technique, CBA). Our data showed that 8-pSPT-treated mice presented an increase in the percentage of DCs as well as of their stimulatory and co-stimulatory molecules (CD40, CD80 and MHCII). Regarding production of T cell cytokines, we observed a significant increase in the levels of IL-2 and a significant decrease in IL-10, IL-17, TNF-α and IFN-γ cytokines.\ud \ud \ud Conclusions\ud These results suggest that ADO produced by ticks helps them feed and reproduce and that this effect may be due to modulation of host DCs and T cells.This work was supported by the Conselho Nacional de Desenvolvimento\ud Científico e Tecnológico (CNPq), fellowship numbers 301,663/2007–6,\ud 308,815/2010–6 and 308,280/2013–0 and São Paulo Research Foundation\ud (FAPESP) grant agreement number 2011/00905–8 and fellowship number\ud 2010/11285–8. The funders had no role in study design, data collection and\ud analysis, decision to publish or preparation of the paper

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ