First Record of \u3ci\u3eOchlerotatus Japonicus\u3c/i\u3e (Diptera: Culicidae) in St. Joseph County, Indiana

A single female specimen of Ochlerotatus japonicus (Theobald)(formerly Aedes japonicus), the Asian bush mosquito, was captured in St. Joseph County, IN on 29 July 2004. This is the first report of that species in northern Indiana. Additional specimens were subsequently collected, indicating probable establishment throughout the county

[CI] and CO in the center of M 51

We present J=2-1, J=3-2, J=4-3 12CO maps as well as J=2-1, J=3-2 13CO and 492 GHz [CI] measurements of the central region in M51. The distribution of CO is strongly concentrated towards the spiral arms. The center itself is poor in, though not devoid of, CO emission. The observed line intensities require modelling with a multi-component molecular gas. A dense component must be present with n(H2) = 1000 cm-3 and kinetic temperature T(kin)= 100 K, combined with either a less dense (about 100 cm-3) component of the same temperature, or a more dense (n(H2) = 3000 cm-3) and much cooler (T(kin) = 10-30 K) component. Atomic carbon amounts are between 5 and 10 times those of CO. Much of the molecular gas mass is associated with the hot PDR phase. The center of M 51 has a face-on gas mass density of about 40+/-20 M(O) pc-2, and a well-established CO-to-H2 conversion ratio X four to five times lower than the standard Galactic value.Comment: Accepted for publication by A&

Cold dust in a selected sample of nearby galaxies. I. The interacting galaxy NGC4631

We have observed the continuum emission of the interacting galaxy NGC4631 at 0.87 and 1.23mm using the Heinrich-Hertz-Telescope on Mt. Graham and the IRAM 30-m telescope on Pico Veleta. We have obtained fully sampled maps which cover the optical emission out to a radius of about 7' at both wavelengths. For a detailed analysis, we carefully subtracted the line contributions and synchrotron and free-free emission from the data, which added up to 6% at 1.23mm and 10% at 0.87mm. We combined the flux densities with FIR data to obtain dust spectra and calculate dust temperatures, absorption cross sections, and masses. Assuming a ``standard'' dust model, which consists of two populations of big grains at moderate and warm temperatures, we obtained temperatures of 18K and 50K for the both components. However, such a model suffers from an excess of the radiation at 1.23mm, and the dust absorption cross section seems to be enhanced by a factor 3 compared to previous results and theoretical expectations. At large galactocentric radii, where the galaxy shows disturbances as a result of gravitational interaction, this effect seems to be even stronger. Some possibilities to resolve these problems are discussed. The data could be explained by a very cold dust component at a temperature of 4-6K, an increased abundance of very small grains, or a component of grains with unusual optical properties. We favour the latter possibility, since the first two lead to inconsistencies.Comment: 12 pages, 6 figures, accepted for publication in Astronomy & Astrophysics. Updated version with minor errors corrected (typos, LaTeX formatting, missing citation

Molecular clouds in the center of M81

We investigate the molecular gas content and the excitation and fragmentation properties in the central region of the spiral galaxy Messier 81 in both the ^{12}CO(1-0) and ^{12}CO(2-1) transitions. We have recently observed the two transitions of CO in the M~81 center with A, B, and HERA receivers of the IRAM 30-m telescope. We find no CO emission in the inner $\sim$ 300 pc and a weak molecular gas clump structure at a distance of around 460 pc from the nucleus. Observations of the first two CO transitions allowed us to compute the line ratio, and the average I_{21}/I_{10} ratio is 0.68 for the M~81 center. This low value, atypical both of the galactic nuclei of spiral galaxies and of interacting systems, is probably associated to diffuse gas with molecular hydrogen density that is not high enough to excite the CO molecules. After analyzing the clumping properties of the molecular gas in detail, we identify very massive giant molecular associations (GMAs) in CO(2-1) emission with masses of $\sim$ 10$^{5}$ M$_\odot$ and diameters of $\sim$ 250 pc. The deduced N(H_{2})/I_{CO} ratio for the individually resolved GMAs, assumed to be virialized, is a factor of $\sim$ 15 higher than the \textit{standard} Galactic value, showing - as suspected - that the X ratio departs significantly from the mean for galaxies with an unusual physics of the molecular gas.Comment: 12 pages, 7 figures, 6 tables. Accepted for pubblication in A&

Molecular gas in the galaxy M83. I - The molecular gas distribution

We present CO(1-0) and CO(2-1) Swedish-ESO Submillimetre Telescope (SEST) observations of the barred spiral galaxy M83 (NGC5236). The maps cover the entire optical disk. The CO emission is strongly peaked toward the nucleus, which breaks up into two separate components in the CO(2-1) data due to the higher spatial resolution. Emission from the bar is strong, in particular on the leading edges of the bar. The molecular gas arms are clearly resolved and can be traced for more than 360\degr . Emission in the inter-arm regions is detected. The average CO CO(2-1)/CO(1-0) line ratio is 0.77. The ratio is lower than this on the spiral arms and higher in the inter-arm regions. The arms show regularly spaced concentrations of molecular gas, Giant Molecular Associations (GMA's), whose masses are of the order 10^7 Msun. The total molecular gas mass is estimated to be 3.9*10^9 Msun. This mass is comparable to the total HI mass, but H_2 dominates in the optical disk. In the disk, H_2 and HI show very similar distributions, including small scale clumping. We compare the molecular gas distribution with those of other star formation tracers, such as B and H_alpha images.Comment: 20 pages, 15 figures, A&A accepted. A higher resolution version available at http://www.astro.su.se/~andreas/publications

Extra-planar gas in the spiral galaxy NGC 4559

We present 21-cm line observations of the spiral galaxy NGC 4559, made with the Westerbork Synthesis Radio Telescope. We have used them to study the HI distribution and kinematics, the relative amount and distribution of luminous and dark matter in this galaxy and, in particular, the presence of extra-planar gas. Our data do reveal the presence of such a component, in the form of a thick disk, with a mass of 5.9 x 10^8 Mo (one tenth of the total HI mass) and a mean rotation velocity 25-50 km/s lower than that of the thin disk. The extra-planar gas may be the result of galactic fountains but accretion from the IGM cannot be ruled out. With this study we confirm that lagging, thick HI layers are likely to be common in spiral galaxies.Comment: 17 pages, 10 figures. Accepted for publication in A&

The Swift/UVOT catalogue of NGC4321 star forming sources: A case against density wave theory

We study the star forming regions in the spiral galaxy NGC4321, taking advantage of the spatial resolution (2.5 arcsec FWHM) of the Swift/UVOT camera and the availability of three UV passbands in the region 1600-3000 A, in combination with optical and IR imaging from SDSS, KPNO/Ha and Spitzer/IRAC, to obtain a catalogue of 787 star forming regions out to three disc scale lengths. We determine the properties of the young stellar component and its relationship with the spiral arms. The Ha luminosities of the sources have a strong decreasing radial trend, suggesting more massive star forming regions in the central part of the galaxy. When segregated with respect to NUV-optical colour, blue sources have a significant excess of flux in the IR at 8 micron, revealing the contribution from PAHs, although the overall reddening of these sources stays below E(B-V)=0.2 mag. The distribution of distances to the spiral arms is compared for subsamples selected according to Ha luminosity, NUV-optical colour, or ages derived from a population synthesis model. An offset is expected between these subsamples as a function of radius if the pattern speed of the spiral arm were constant - as predicted by classic density wave theory. No significant offsets are found, favouring instead a mechanism where the pattern speed has a radial dependence.Comment: 12 pages, 11 figures, 4 tables. MNRAS, in pres

Sensitive and selective amplification of methylated DNA sequences using helper-dependent chain reaction in combination with a methylation-dependent restriction enzyme

We have developed a novel technique for specific amplification of rare methylated DNA fragments in a high background of unmethylated sequences that avoids the need of bisulphite conversion. The methylation-dependent restriction enzyme GlaI is used to selectively cut methylated DNA. Then targeted fragments are tagged using specially designed ‘helper’ oligonucleotides that are also used to maintain selection in subsequent amplification cycles in a process called ‘helper-dependent chain reaction’. The process uses disabled primers called ‘drivers’ that can only prime on each cycle if the helpers recognize specific sequences within the target amplicon. In this way, selection for the sequence of interest is maintained throughout the amplification, preventing amplification of unwanted sequences. Here we show how the method can be applied to methylated Septin 9, a promising biomarker for early diagnosis of colorectal cancer. The GlaI digestion and subsequent amplification can all be done in a single tube. A detection sensitivity of 0.1% methylated DNA in a background of unmethylated DNA was achieved, which was similar to the well-established Heavy Methyl method that requires bisulphite-treated DNA.Funding for open access charge: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia. National Health & Medical Research Counci

Molecular gas in late-type galaxies

We present $^{12}$CO(J=1--0) line observations of 22 low-luminosity spiral galaxies in the Virgo cluster. These data, together with 244 others available in the literature, allow us to build a large sample that we use to study the molecular gas properties of galaxies spanning a large range of morphological types and luminosities and belonging to different environments (clusters - field). The molecular gas content of the target galaxies is estimated using a luminosity-dependent X = $N(H_2)/I(CO)$ conversion factor that has been calibrated on a sample of nearby galaxies. $X$ spans from $\sim$ 10$^{20}$ mol cm$^{-2}$ (K km s$^{-1})^{-1}$ in giant spirals to $\sim$ 10$^{21}$ mol cm$^{-2}$ (K km s$^{-1})^{-1}$ in dwarf irregulars. The value of the $X$ conversion factor is found consistent with a value derived independently from dust masses estimated from FIR fluxes, with a metallicity-dependent dust to gas ratio. The relationships between X and the UV radiation field (as traced by the $H\alpha+[NII] E.W.$), the metallicity and the H band luminosity are analysed. We show that the molecular gas contained in molecular clouds or complexes is of the order of 15% of the total gas on average whatever the luminosity or the Hubble type of the galaxies. We discuss the relation between the star formation rate and the molecular gas content and estimate the average star formation efficiency of late-type galaxies.Comment: accepted for publication on Astronomy and Astrophysic