6 research outputs found
The third international hackathon for applying insights into large-scale genomic composition to use cases in a wide range of organisms
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Induced-expression of osmolyte biosynthesis pathway genes improves salt and oxidative stress tolerance in <em>Brassica</em> species
711-721Osmolytes and other compatible solutes help to improve abiotic stress tolerance by modulating different physiological and metabolic processes in plants. In the present study, we assessed the relative role of antioxidant enzyme mediated defence system and osmotic adjustment mechanism for salinity stress tolerance in Brassica species. For this, a pot experiment was carried out with four Brassica genotypes from two species (B. juncea and B. campestris) and three different levels of salinity treatment. Salinity stress resulted in significant build-up of oxidative stress level (H2O2 and superoxide radical content, and lipid peroxidation) with the progressive increase in soil salinity. Relatively tolerant cultivars, CS 52 and CS 54 showed lesser oxidative stress and higher antioxidant enzymes activities (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and glutathione reductase) under the highest level of salt stress. Accumulation of organic osmolyte viz. glycine betaine and trehalose increased sharply under salinity stress especially in B. juncea cultivars. Gene expressions of BADH and T6PS, which regulates the biosynthesis of glycine betaine and trehalose were higher in B. juncea cultivars (CS 52 and CS 54) than T 9 (B. campestris). The results of the present study clearly showed a definite role of both osmoprotection and enzyme driven antioxidant defence mechanism as the basis of salt-tolerance in these Brassica genotypes and their simultaneous induction possibly brought more favourable cellular environment and salt tolerance in CS 52 and CS 54
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Not AvailableOsmolytes and other compatible solutes help to improve abiotic stress tolerant by modulation different psychological and metabolic process in plants. in the present study , we assessd the relative role of antioxidant enzyme mediated defence system and osmotic adjustment mechanism for salinity stress tolerant in Brassica species.Not Availabl