A comparison of substrate oxidation during prolonged exercise in men at terrestrial altitude and normobaric normoxia following the coingestion of 13C glucose and 13C fructose

This study compared the effects of co-ingesting glucose and fructose on exogenous and endogenous substrate oxidation during prolonged exercise at altitude and sea level, in men. Seven male British military personnel completed two bouts of cycling at the same relative workload (55% Wmax) for 120 minutes on acute exposure to altitude (3375m) and at sea level (~113m). In each trial, participants ingested 1.2 g.min-1 of glucose (enriched with 13C glucose) and 0.6 g.min-1 of fructose (enriched with 13C fructose) directly before and every 15 minutes during exercise. Indirect calorimetry and isotope ratio mass spectrometry were used to calculate fat oxidation, total and exogenous carbohydrate oxidation, plasma glucose oxidation and endogenous glucose oxidation derived from liver and muscle glycogen. Total carbohydrate oxidation during the exercise period was lower at altitude (157.7±56.3 grams) than sea level (286.5±56.2 grams, P=0.006, ES=2.28), whereas fat oxidation was higher at altitude (75.5±26.8 grams) than sea level (42.5±21.3 grams, P=0.024, ES=1.23). Peak exogenous carbohydrate oxidation was lower at altitude (1.13±0.2 g.min-1) than sea level (1.42±0.16 g.min-1, P=0.034, ES=1.33). There were no differences in rates, or absolute and relative contributions of plasma or liver glucose oxidation between conditions during the second hour of exercise. However, absolute and relative contributions of muscle glycogen during the second hour were lower at altitude (29.3±28.9 grams, 16.6±15.2%) than sea level (78.7±5.2 grams (P=0.008, ES=1.71), 37.7±13.0% (P=0.016, ES=1.45). Acute exposure to altitude reduces the reliance on muscle glycogen and increases fat oxidation during prolonged cycling in men, compared with sea level

Pl@ntNet, une plate-forme innovante d'agrégation et partage d'observations botaniques

Pl@ntNet est un réseau humain s'appuyant sur une infrastructure informatique, permettant l'identification, l'agrégation et le partage d'observations botaniques à très grande échelle. Cette initiative mobilise différentes institutions de recherche dans divers champs scientifiques (informatique, agronomie, écologie) et de larges réseaux associatifs de naturalistes ; elle a permis au cours des 20 derniers mois la collecte de plusieurs dizaines de milliers d'observations de plantes sur le territoire européen. Celles-ci sont collectées à travers un système web ou mobile d'aide à l'identification des plantes par l'image, au sein duquel chacun des participants peut partager des observations déterminées ou non, avec différents niveaux d'informations. Ces observations, sont ensuite révisées collaborativement à travers 2 applications web, l'une dédiée à la révision de la détermination des observations (IdentiPlante), l'autre à l'estimation de la qualité visuelle des images illustrant l'observation (PictoFlora). Les observations révisées et / ou validées, viennent alors enrichir la base d'observations publiques exploitée par le système d'aide à l'identification par l'image. Bien que cette infrastructure logicielle soit encore récente (lancement des applications mobiles en févier 2013), elle a été exploitée au cours des 20 derniers mois par près de 300 000 utilisateurs à travers le monde. Les modalités de fonctionnement de celle-ci, ainsi que les perspectives d'évolution sont présentées dans cet article, qui se conclut par une discussion sur les changements actuels que les nouvelles technologies de l'information permettent d'opérer en Botanique. (Résumé d'auteur

Identification expérimentale du comportement d'un fuselage composite : détection de défauts par mesures de champs

Le contexte de ce travail concerne le process d'Infusion de Résine Liquide (LRI) développé dans le cadre du projet « FUSelage COMPosite » par DAHER SOCATA. Ce process de fabrication permet de réaliser des pièces de formes complexes et des panneaux entiers de fuselage en composites, ce qui réduit considérablement les étapes d'assemblages et donc les temps de production. Les travaux de thèse portent sur l'identification expérimentale du comportement d'un fuselage composite. Ce travail se divise en deux parties qui sont la qualification du contrôle non destructif (CND) par rapport à une taille de défaut critique et l'identification du comportement d'une structure composite orthotrope en présence de ce défaut. Le premier volet consiste à évaluer les techniques de CND basées sur des mesures de champs (acoustiques, thermiques et densimétriques), capables de détecter des défauts internes de types délaminage et porosité au sein de structures composites monolithiques et sandwichs, et fournissant une visualisation des résultats par une cartographie de défauts 2D ou 3D. Le choix de ces méthodes a été motivé par la volonté de DAHER SOCATA d'acquérir de nouvelles compétences en matière de CND. Le deuxième volet consiste à évaluer les paramètres élastiques d'une structure composite orthotrope (structure comprenant une zone saine et une zone localement dégradée) via une procédure d'identification, à partir de mesures de champs, globale et locale par recalage de modèles éléments finis. Cette procédure se décompose en quatre parties avec tout d'abord l'identification des propriétés de la structure saine, ensuite la localisation de la zone dégradée, l'intégration de celle-ci dans le modèle éléments finis, et l'identification des propriétés de cette dernière. ABSTRACT : The context of this work concerns the process of Liquid Resin Infusion (LRI) developed under the project "composite fuselage" by DAHER SOCATA. This manufacturing process can produce parts with complex shapes and entire panels of composite fuselage, reducing assembly steps and therefore the production time. The thesis work focused on the experimental identification of the behavior of a composite fuselage. This work is divided into two parts which are the qualification of non destructive testing (NDT) compared to a critical defect size and the identification of the behavior of an orthotropic composite structure with defect. The first part is to evaluate the NDT techniques based on full field measurements (acoustic, thermal and densimetric), capable of detecting internal defects as porosities and delaminations in monolithic and sandwich composite structures, and providing a results visualization by a 2D or 3D defects map. The choice of these methods was motivated by DAHER SOCATA which wants to learn new NDT skills. The second part is to evaluate the elastic parameters of an orthotropic composite structure (structure composed by a virgin zone and a damaged zone) through an identification process from field measurements, by global and local step. This procedure is divided into four parts with two identification steps and a image processing step

High dietary fat intake increases fat oxidation and reduces skeletal muscle mitochondrial respiration in trained humans.

High-fat, low-carbohydrate (CHO) diets increase whole-body rates of fat oxidation and down-regulate CHO metabolism. We measured substrate utilization and skeletal muscle mitochondrial respiration to determine whether these adaptations are driven by high fat or low CHO availability. In a randomized crossover design, 8 male cyclists consumed 5 d of a high-CHO diet [>70% energy intake (EI)], followed by 5 d of either an isoenergetic high-fat (HFAT; >65% EI) or high-protein diet (HPRO; >65% EI) with CHO intake clamped at <20% EI. During the intervention, participants undertook daily exercise training. On d 6, participants consumed a high-CHO diet before performing 100 min of submaximal steady-state cycling plus an ∼30-min time trial. After 5 d of HFAT, skeletal muscle mitochondrial respiration supported by octanoylcarnitine and pyruvate, as well as uncoupled respiration, was decreased at rest, and rates of whole-body fat oxidation were higher during exercise compared with HPRO. After 1 d of high-CHO diet intake, mitochondrial respiration returned to baseline values in HFAT, whereas rates of substrate oxidation returned toward baseline in both conditions. These findings demonstrate that high dietary fat intake, rather than low-CHO intake, contributes to reductions in mitochondrial respiration and increases in whole-body rates of fat oxidation after a consuming a high-fat, low-CHO diet.-Leckey, J. J., Hoffman, N. J., Parr, E. B., Devlin, B. L., Trewin, A. J., Stepto, N. K., Morton, J. P., Burke, L. M., Hawley, J. A. High dietary fat intake increases fat oxidation and reduces skeletal muscle mitochondrial respiration in trained humans

Vers une approche panlectale de la variation du français, de Robert Chaudenson, Raymond Mougeon et Édouard Beniak.

Non

Low carbohydrate, high fat diet impairs exercise economy and negates the performance benefit from intensified training in elite race walkers

We investigated the effects of adaptation to a ketogenic low carbohydrate (CHO), high fat diet (LCHF) during 3 weeks of intensified training on metabolism and performance of world-class endurance athletes. We controlled three isoenergetic diets in elite race walkers: high CHO availability (g kg−1 day−1: 8.6 CHO, 2.1 protein, 1.2 fat) consumed before, during and after training (HCHO, n = 9); identical macronutrient intake, periodised within or between days to alternate between low and high CHO availability (PCHO, n = 10); LCHF (< 50 g day−1 CHO; 78% energy as fat; 2.1 g kg−1 day−1 protein; LCHF, n = 10). Post-intervention, ˙V O2peak during race walking increased in all groups (P < 0.001, 90% CI: 2.55, 5.20%). LCHF was associated with markedly increased rates of whole-body fat oxidation, attaining peak rates of 1.57±0.32 gmin−1 during 2 h of walking at 80% ˙V O2peak.However, LCHFalso increased the oxygen (O2) cost of race walking at velocities relevant to real-life race performance: O2 uptake (expressed as a percentage of new ˙V O2peak) at a speed approximating 20 km race pace was reduced in HCHO and PCHO (90% CI:−7.047,−2.55 and−5.18,−0.86, respectively), but was maintained at pre-intervention levels in LCHF. HCHO and PCHO groups improved times for 10 km race walk: 6.6% (90% CI: 4.1, 9.1%) and 5.3% (3.4, 7.2%), with no improvement (−1.6% (−8.5, 5.3%)) for the LCHF group. In contrast to training with diets providing chronic or periodised high-CHO availability, and despite a significant improvement in ˙V O2peak, adaptation to the topical LCHF diet negated performance benefits in elite endurance athletes, in part due to reduced exercise economy

Pattern de pigmentation et contraintes développementales: les sites d'attachement des muscles du vol délimitent le trident thoracique de Drosophila melanogaster

International audienceIn their seminal paper published in 1979, Gould and Lewontin argued that some traits arise as by-products of the development of other structures and not for direct utility in themselves. We show here that this applies to the trident, a pigmentation pattern observed on the thorax of Drosophila melanogaster. Using reporter constructs, we show that the expression domain of several genes encoding pigmentation enzymes follows the trident shape. This domain is complementary to the expression pattern of stripe (sr), which encodes an essential transcription factor specifying flight muscle attachment sites. We demonstrate that sr limits the expression of these pigmentation enzyme genes to the trident by repressing them in its own expression domain, i.e. at the flight muscle attachment sites. We give evidence that repression of not only yellow but also other pigmentation genes, notably tan, is involved in the trident shape. The flight muscle attachment sites and sr expression patterns are remarkably conserved in dipterans reflecting the essential role of sr. Our data suggest that the trident is a by-product of flight muscle attachment site patterning that arose when sr was co-opted for the regulation of pigmentation enzyme coding genes

Prolonged constant load cycling exercise is associated with reduced gross efficiency and increased muscle oxygen uptake

This study investigated the effects of prolonged constant load cycling exercise on cycling efficiency and local muscle oxygen uptake responses. Fourteen well-trained cyclists each completed a 2-h steady-state cycling bout at 60% of their maximal minute power output to assess changes in gross cycling efficiency (GE) and muscle oxygen uptake (mVO2) at time points 5, 30, 60, 90, and 120 min. Near-infrared spatially resolved spectroscopy (NIRS) was used to continually monitor tissue oxygenation of the Vastus Lateralis muscle, with arterial occlusions (OCC) applied to assess mVO2 . The half-recovery time of oxygenated hemoglobin (HbO2 ) was also assessed pre and post the 2-h cycling exercise by measuring the hyperemic response following a 5-min OCC. GE significantly declined during the 2-h cycling bout (18.4 ± 1.6 to 17.4 ± 1.4%; P < 0.01). Conversely, mVO2 increased, being significantly higher after 90 and 120 min than at min 5 (+0.04 mlO2 /min/100 g; P = 0.03). The half-recovery time for HbO2 was increased comparing pre and post the 2-h cycling exercise (+7.1 ± 19s), albeit not significantly (d: 0.48; P = 0.27). This study demonstrates that GE decreases during prolonged constant load cycling exercise and provides evidence of an increased mVO2 , suggestive of progressive mitochondrial or contractile inefficiency

The MAP kinase ERK and its scaffold protein MP1 interact with the chromatin regulator Corto during Drosophila wing tissue development

<p>Abstract</p> <p>Background</p> <p>Mitogen-activated protein kinase (MAPK) cascades (p38, JNK, ERK pathways) are involved in cell fate acquisition during development. These kinase modules are associated with scaffold proteins that control their activity. In <it>Drosophila</it>, <it>dMP1</it>, that encodes an ERK scaffold protein, regulates ERK signaling during wing development and contributes to intervein and vein cell differentiation. Functional relationships during wing development between a chromatin regulator, the Enhancer of Trithorax and Polycomb Corto, ERK and its scaffold protein dMP1, are examined here.</p> <p>Results</p> <p>Genetic interactions show that <it>corto </it>and <it>dMP1 </it>act together to antagonize <it>rolled </it>(which encodes ERK) in the future intervein cells, thus promoting intervein fate. Although Corto, ERK and dMP1 are present in both cytoplasmic and nucleus compartments, they interact exclusively in nucleus extracts. Furthermore, Corto, ERK and dMP1 co-localize on several sites on polytene chromosomes, suggesting that they regulate gene expression directly on chromatin. Finally, Corto is phosphorylated. Interestingly, its phosphorylation pattern differs between cytoplasm and nucleus and changes upon ERK activation.</p> <p>Conclusions</p> <p>Our data therefore suggest that the Enhancer of Trithorax and Polycomb Corto could participate in regulating vein and intervein genes during wing tissue development in response to ERK signaling.</p

Phenotypic Plasticity in Drosophila Pigmentation Caused by Temperature Sensitivity of a Chromatin Regulator Network

Phenotypic plasticity is the ability of a genotype to produce contrasting phenotypes in different environments. Although many examples have been described, the responsible mechanisms are poorly understood. In particular, it is not clear how phenotypic plasticity is related to buffering, the maintenance of a constant phenotype against genetic or environmental variation. We investigate here the genetic basis of a particularly well described plastic phenotype: the abdominal pigmentation in female Drosophila melanogaster. Cold temperature induces a dark pigmentation, in particular in posterior segments, while higher temperature has the opposite effect. We show that the homeotic gene Abdominal-B (Abd-B) has a major role in the plasticity of pigmentation in the abdomen. Abd-B plays opposite roles on melanin production through the regulation of several pigmentation enzymes. This makes the control of pigmentation very unstable in the posterior abdomen, and we show that the relative spatio-temporal expression of limiting pigmentation enzymes in this region of the body is thermosensitive. Temperature acts on melanin production by modulating a chromatin regulator network, interacting genetically with the transcription factor bric-à-brac (bab), a target of Abd-B and Hsp83, encoding the chaperone Hsp90. Genetic disruption of this chromatin regulator network increases the effect of temperature and the instability of the pigmentation pattern in the posterior abdomen. Colocalizations on polytene chromosomes suggest that BAB and these chromatin regulators cooperate in the regulation of many targets, including several pigmentation enzymes. We show that they are also involved in sex comb development in males and that genetic destabilization of this network is also strongly modulated by temperature for this phenotype. Thus, we propose that phenotypic plasticity of pigmentation is a side effect reflecting a global impact of temperature on epigenetic mechanisms. Furthermore, the thermosensitivity of this network may be related to the high evolvability of several secondary sexual characters in the genus Drosophila