31 research outputs found

    The microbial ferrous wheel in a neutral pH groundwater seep

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    Evidence for microbial Fe redox cycling was documented in a circumneutral pH ground-water seep near Bloomington, Indiana. Geochemical and microbiological analyses were conducted at two sites, a semi-consolidated microbial mat and a floating puffball structure. In situ voltammetric microelectrode measurements revealed steep opposing gradients of o2 and Fe(I I) at both sites, similar to other groundwater seep and sedimentary environments known to support microbial Fe redox cycling. The puffball structure showed an abrupt increase in dissolved Fed I) just at its surface (~5cm depth), suggesting an internal Fe(I I) source coupled to active Fed 1I) reduction. Most probable number enumerations detected microaerophilic Fe(II)-oxidizing bacteria (FeoB) and dissimilatory Fe(III)-reducing bacteria (FeRB) at densities of 102 to 105 cells ml_~1 in samples from both sites. In vitro Fed 1I) reduction experiments revealed the potential for immediate reduction (no lag period) of native Fe(III) oxides. Conventional full-length 16S rRNA gene clone libraries were compared with high throughput barcode sequencing of theV1, V4, orV6 variable regions of 16S rRNA genes in order to evaluate the extent to which new sequencing approaches could provide enhanced insight into the composition of Fe redox cycling microbial community structure. The composition of the clone libraries suggested a lithotroph-dominated microbial community centered around taxa related to known FeoB (e.g., Gallionella, Sideroxydans, Aquabacterium). Sequences related to recognized FeRB (e.g., Rhodoferax, Aeromonas, Geobacter, Desulfovibrio) were also well-represented. overall, sequences related to known FeoB and FeRB accounted for 88 and 59% of total clone sequences in the mat and puffball libraries, respectively. Taxa identified in the barcode libraries showed partial overlap with the clone libraries, but were not always consistent across different variable regions and sequencing platforms. However, the barcode libraries provided confirmation of key clone library results (e.g., the predominance of Betaproteobacteria) and an expanded view of lithotrophic microbial community composition

    Uranium mobility in organic matter-rich sediments: A review of geological and geochemical processes

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    Uranium (U) is of enormous global importance because of its use in energy generation, albeit with potential environmental legacies. While naturally occurring U is widespread in the Earth's crust at concentrations of ~1 to 3 ppm, higher concentrations can be found, includingwithin organicmatter (OM)-rich sediments, leading to economic extraction opportunities. The primary determinants of U behaviour in ore systems are pH, Eh, U oxidation state (U(IV), U(VI)) and the abundance of CO3 2– ions. The concentration/availability and interrelationships among such determinants vary, and the solubility and mobility of ions (e.g. OH-, CO3 2–, PO4 3-, SiO4 4-, SO4 2-) that compete for U (primarily as U(VI)) will also influence the mobility of U. In addition, the presence of OM can influence U mobility and fate by the degree of OMsorption to mineral surfaces (e.g. Fe- and Si- oxides and hydroxides). Within solid-phase OM, microbes can influence U oxidation state and U stability through direct enzymatic reduction, biosorption, biomineralisation and bioaccumulation. The biogenic UO2 product is, however, reported to be readily susceptible to reoxidation and therefore more likely remobilised over longer time periods. Thus several areas of uncertainty remain with respect to factors contributing to U accumulation, stability and/or (re)mobilisation. To address these uncertainties, this paper reviews U dynamics at both geological and molecular scales. Here we identify U-OMbond values that are in agreement, relatively strong, independent from ionic strength and which may facilitate either U mobilisation or immobilisation, depending on environmental conditions. We also examine knowledge gaps in the literature, with U-OM solubility data generally lacking in comparison to data for U sorption and dissolution, and little information available on multi-component relationships, such as UOM-V (V as vanadate). Furthermore, the capability ofOMto influence the oxidation state of U at near surface conditions remains unclear, as it can be postulated that electron shuttling by OM may contribute to changes in U redox state otherwise mediated by bacteria. Geochemical modelling of the environmental mobility of U will require incorporation of data from multi-corporation studies, as well as from studies of U-OM microbial interactions, all of which are considered in this review

    Iron isotope geochemistry of biogenic magnetite-bearing sediments from the Bay of Vidy, Lake Geneva

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    Dissimilatory microbial iron oxide reduction (DIR) has been hypothesized to be an important respiratory pathway on early Earth, potentially generating significant quantities of Fe(II) that have been preserved in Proterozoic and Archean sedimentary rocks. In particular, DIR has been implicated in the formation of magnetite in Precambrian marine sediments. To date, however, only one modern sedimentary environment existswhere in situ magnetite formation has been linked to DIR: the Bay of Vidy in Lake Geneva, Switzerland. Previous work at this locality has characterized a magnetic susceptibility anomaly that reflects the presence of fine-grained magnetite produced via microbial reduction of amorphous Fe(III) oxides that enter the Bay of Vidy froma nearby sewage treatment plant. In this study, we report on the Fe isotope composition of aqueous and solid-phase Fe in the Bay of Vidy sediments. Extensive Fe(III) reduction has occurred, resulting in the conversion of nearly all reactive (non-silicate) Fe(III) to a variety of Fe(II)-bearing phases, with mixed Fe valence magnetite being a minor but easily detectable component (0.5–8wt.%). Very little Fe isotope variation was observed in any solid phase Fe components, including magnetite, although significant fractionation was observed between aqueous and solid-phase Fe(II). Because Fe mass-balance was dominated by the solid phase, little net change in δ56Fe values for Fe(II)-bearing components was produced despite clear evidence for DIR. This study provides a basis for interpreting instances in the rock recordwhere DIR was the driving force for Fe(II) production and magnetite formation, yet no significant deviations in δ56Fe valueswere preserved. A key implication of the results is that Fe isotope homogeneity is not sufficient to rule out a biological mechanismfor magnetite formation, and this should be taken into account when examining the Precambrian rock record

    The microbial ferrous wheel in a neutral pH groundwater seep

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    Evidence for microbial Fe redox cycling was documented in a circumneutral pH groundwater seep near Bloomington, Indiana. Geochemical and microbiological analyses were conducted at two sites, a semi-consolidated microbial mat and a floating puffball structure. In situ voltammetric microelectrode measurements revealed steep opposing gradients of O2 and Fe(II) at both sites, similar to other groundwater seep and sedimentary environments known to support microbial Fe redox cycling. The puffball structure showed an abrupt increase in dissolved Fe(II) just at its surface (~ 5 cm depth), suggesting an internal Fe(II) source coupled to active Fe(III) reduction. MPN enumerations detected microaerophilic Fe(II)-oxidizing bacteria (FeOB) and dissimilatory Fe(III)-reducing bacteria (FeRB) at densities of 102-105 cells mL-1 in samples from both sites. In vitro Fe(III) reduction experiments revealed the potential for immediate reduction (no lag period) of native Fe(III) oxides. Conventional full-length 16S rRNA gene clone libraries were compared withhigh throughput barcode sequencing of the V1, V4 or V6 variable regions of 16S rRNA genes in order to evaluate the extent to which new sequencing approaches could provide enhanced insight into the composition of Fe redox cycling microbial community structure. The composition of the clone libraries suggested a lithotroph-dominated microbial community centered around taxa related to known FeOB (e.g. Gallionella, Sideroxydans, Aquabacterium). Sequences related to recognized FeRB (e.g. Rhodoferax, Aeromonas, Geobacter, Desulfovibrio) were also well represented. Overall, sequences related to known FeOB and FeRB accounted for 88 and 59% of total clone sequences in the mat and puffball libraries, respectively. Taxa identified in the barcode libraries showed partial overlap with the clone libraries, but were not always consistent across different variable regions and sequencing platforms. However, the barcode libraries provided confirmati
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