AVALIAÇÃO DA ATIVIDADE ANTINEOPLÁSICA E ANTIPROLIFERATIVA DE EXTRATOS DE PLANTAS SOBRE TUMOR MAMÁRIO DE LINHAGEM 4T1

Uma alternativa terapêutica promissora, mas ainda incipiente, para o tratamento detumores mamários é a utilização de plantas medicinais e de extratos vegetaisbioativos. Nesse sentido, este projeto tem por objetivo avaliar o potencial terapêuticodos extratos de plantas pertencentes às famílias botânicas Fabaceae, Rubiaceae,Arecaceae. Para tanto, foram realizados testes in vitro para determinar acitotoxicidade, o potencial antineoplásico e antiproliferativo, além de possível atividadeantimicrobiana. Cultivos celulares de linhagem primária leucocítica, de linhagemcontínua HUT-78 e de células de tumor mamário da linhagem 4T1 (para os testes deeficácia e mecanismos efetores) foram submetidos a tratamento com 3 diferentesconcentrações de cada extrato de planta, em padrão de ensaio em triplicata. Acapacidade proliferativa foi avaliada através de contagens manuais com o uso dacâmara de Neubauer. A visualização morfológica se deu pelo uso da câmara deNeubauer e o corante Trypan blue. Foram realizadas análises estatísticas quanto aocrescimento padrão das células de linhagem primária e sua resposta mediante otratamento com os extratos. As células da linhagem 4T1 foram tripsinizadas, tratadase, posteriormente, as atividades citotóxicas foram analisadas com o uso do kit TheCytoTox-Glo™, um ensaio enzimático luminescente, que permite mensurar o númerode células mortas em uma população celular previamente cultivada, através daatividade de uma protease distinta, com um peptídeo luminescente que identifica esinaliza principalmente a integridade da membrana celular (determinando se a mortecelular ocorreu por necrose ou apoptose). Comprovou-se a atividadeantineoplásica/antiproliferativa e determinou-se a concentração ideal, qual seja, aconcentração minimamente citotóxica in vitro, mas completamente eficaz contra ascélulas neoplásicas. Foram realizados, outrossim, breves ensaios com culturasbacterianas para observar se os extratos, em diferentes concentrações, possuempossível atividade antimicrobiana em diferentes cepas bacteriana

Troponin in COVID-19: To Measure or Not to Measure? Insights from a Prospective Cohort Study

Myocardial injury (MI), defined by troponin elevation, has been associated with increased mortality and adverse outcomes in patients with coronavirus disease 2019 (COVID-19), but the role of this biomarker as a risk predictor remains unclear. Data from adult patients hospitalized with COVID-19 were recorded prospectively. A multiple logistic regression model was used to quantify associations of all variables with in-hospital mortality, including the calculation of odds ratios (ORs) and confidence intervals (CI). Troponin measurement was performed in 1476 of 4628 included patients, and MI was detected in 353 patients, with a prevalence of 23.9%; [95% CI, 21.8–26.1%]. The total in-hospital mortality rate was 10.9% [95% CI, 9.8–12.0%]. The mortality was much higher among patients with MI than among those without MI, with a prevalence of 22.7% [95% CI, 18.5–27.3%] vs. 5.5% [95% CI, 4.3–7.0%] and increased with each troponin level. After adjustment for age and comorbidities, the model revealed that the mortality risk was greater for patients with MI [OR = 2.99; 95% CI, 2.06–4.36%], and for those who did not undergo troponin measurement [OR = 2.2; 95% CI, 1.62–2.97%], compared to those without MI. Our data support the role of troponin as an important risk predictor for these patients, capable of discriminating between those with a low or increased mortality rate. In addition, our findings suggest that this biomarker has a remarkable negative predictive value in COVID-19

Relações de saúde e trabalho em assentamento rural do MST na região de fronteira Brasil-Paraguai Health and work relations in a rural settlement of the MST in the Brazil-Paraguay border region

Com o presente artigo, busca-se relatar e analisar as relações entre saúde e trabalho de pequenos produtores rurais do Movimento dos Trabalhadores Rurais Sem Terra (MST). Quanto à metodologia, adotou-se o aporte da investigação participativa e as bases da educação popular. O estudo realizado tornou possível uma compreensão de saúde e de trabalho mediante as narrativas dos trabalhadores e histórias relativas às lutas coletivas contra a exclusão e a violência no campo. Constataram-se importantes aspectos próprios a esse grupo populacional, como o sentido de conquista da propriedade da terra, os conflitos advindos da 'militância' e as diferenças de gênero e geração. Com base nos relatos, analisaram-se também os acidentes e os riscos relacionados à atividade de trabalho no assentamento rural, principalmente a exposição ao agrotóxico. Confirmaram-se, ainda, a solidariedade e a cooperação como valores importantes e como base de novas relações, um modo distinto de organização social coletiva do trabalho.<br>In this article, we aim to report on and analyze the relationship between health and work of small farmers of the Landless Workers Movement (MST). So far as the methodology is concerned, we used participatory research and the basics of popular education. The study allowed for an understanding of health and work based on the workers' narratives, stories relating to collective struggles against exclusion and violence in the countryside. Important aspects related to this population group as were discovered, such as, for example, the sense of conquest of land ownership, the conflicts arising from 'activism,' and the differences of gender and generations. From the reports that were provided, we also examined the accidents and risks related to work in the rural settlement, particularly exposure to pesticides. It was also found that solidarity and cooperation are important values, basic elements for new relations and a distinctive mode of social organization of collective

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved

NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics

Xenarthrans—anteaters, sloths, and armadillos—have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n = 5,941), and Cyclopes sp. have the fewest (n = 240). The armadillo species with the most data is Dasypus novemcinctus (n = 11,588), and the fewest data are recorded for Calyptophractus retusus (n = 33). With regard to sloth species, Bradypus variegatus has the most records (n = 962), and Bradypus pygmaeus has the fewest (n = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans data set. Please cite this data paper when using its data in publications. We also request that researchers and teachers inform us of how they are using these data