Holocene tree-line variability in the Kauner Valley, Central Eastern Alps, indicated by dendrochronological analysis of living trees and subfossil logs

The altitude of the Alpine tree-line has often been used as proxy for the climatic conditions in the Holocene epoch. The usual approach for establishing a record for this proxy is the analysis of pollen and macro remains. We analysed living trees and subfossil logs from the timberline ecotone in the innermost Kauner valley in the Central Eastern Alps in order to assemble a Holocene dendrochronological tree-line record. Data sets comprising age and height of living Stone Pines (Pinus cembra L.) were collected at one site. Sections of 170 subfossil Stone Pine logs from five other sites were dendrochronologically analysed and dated. Besides using dendrochronological analyses, radiocarbon dating served as a means of obtaining the age of some logs. For most of the samples we could provide dendrochronological dates (1-year dating precision, back to 5125 B.C.) or wiggle matched dates (between approx. 7100 and 5040 B.C., dating precision with 95% probability: +/- 7 years). In the first half of the 19th century the tree-line was located at about 2180 m a.s.l. in the innermost Kauner valley. After approximately A.D. 1860 the altitude of the upper limit of the occurrence of Pinus cembra individuals (tree-species-line) and, being closely linked, also that of the tree-line both rose. The current tree-line (trees > 2 m) is located at 2245 m a.s.l. due to climatic conditions around 1980. Additionally we observed saplings up to a present (A.D. 2000) tree-species-line at approx. 2370 m a.s.l. The dendrochronologically analysed subfossil logs found at up to 2410 m a.s.l. date from within the last 9000 years (between approx. 7100 B.C. and A.D. 1700). In the space of the last 4000 years the dendrochronological tree-line record is not continuous, probably due to human impact. Tree-line positions similar to or slightly above the 1980 tree-line are established for the time periods approx. 1000 to 640 B.C. and A.D. 1 to 330 respectively. For the time period between approx. 7100 and 2100 B.C. the dendrochronologically analysed logs show nearly continuous evidence of a tree-line above the 1980s limit. Very high elevation of the tree-line, between 120 and 165 m above the 1980s level (2245 m a.s.l.) and even higher than the A.D. 2000 tree-species-line (2370 m a.s.l.), are recorded for the periods 7090-6570, 6040-5850, 5720-5620, 5500-4370 B.C., approx. 3510-3350 B.C. and 2790-2590 B.C. Additionally, a tree-line which was located at least 50 m above the 1980s limit can be shown for the periods 6700-5430, 4920-3350 and 3280-2110 B.C. The dendrochronological record from the Kauner valley, showing high and very high tree-line positions between approx. 7100 and 2100 B.C. with only two gaps (around 6490 B.C. and from 3350 to 3280 B.C.), suggests that summer temperatures as observed in the late 20th century were at the normal or the lower limit of the temperature range which can be assumed for long periods of the early and middle Holocene epoch

Assessment of penetration of Ascorbyl Tetraisopalmitate into biological membranes by molecular dynamics

The present work, involves the simulation of the transport of a vitamin C derivative, Ascorbyl Tetraisopalmitate (ATI), through human skin by molecular dynamics. Percutaneous absorption of the ATI molecule through the infundibulum, an important route of absorption into the hair follicle of the human skin, has been modeled and compared with the stratum corneum membrane. The comparative study was done, using molecular dynamics with Martini force field. In infundibulum, a single ATI molecule require more time to penetrate, and the data obtained suggested that a high concentration of ATI molecule accelerated the process of penetration. In conclusion, the ATI molecule was found to have more affinity towards the stratum corneum as compared towards the infundibulum and it followed a straight pathway to penetrate (until 600 ns of simulation). In infundibulum, it showed less affinity, more mobility and followed a lateral pathway. Thus, this work contributes to a better understanding of the different molecular interactions during percutaneous absorption of active molecules in these two different types of biological membranes.The authors acknowledge financial support from the Brazilian agencies CAPES, Finep and Fapesp (Project FINEP 01.10.0661-00, FAPESP 2011/13250-0, FAPESP 2013/17247-9, FAPESP 2014/05975-2, CAPES 88887068264/2014-00), of Institute of Research and Development, University of Vale Paraíba

The Business Model: Recent Developments and Future Research

This article provides a broad and multifaceted review of the received literature on business models in which the authors examine the business model concept through multiple subject-matter lenses. The review reveals that scholars do not agree on what a business model is and that the literature is developing largely in silos, according to the phenomena of interest of the respective researchers. However, the authors also found emerging common themes among scholars of business models. Specifically, (1) the business model is emerging as a new unit of analysis; (2) business models emphasize a system-level, holistic approach to explaining how firms “do business”; (3) firm activities play an important role in the various conceptualizations of business models that have been proposed; and (4) business models seek to explain how value is created, not just how it is captured. These emerging themes could serve as catalysts for a more unified study of business models

Targeting and translocation of the two lipoproteins in Escherichia coli via the SRP/Sec/YidC pathway.

In Escherichia coli, two main protein targeting pathways to the inner membrane exist: the SecB pathway for the essentially posttranslational targeting of secretory proteins and the SRP pathway for cotranslational targeting of inner membrane proteins (IMPs). At the inner membrane both pathways converge at the Sec translocase, which is capable of both linear transport into the periplasm and lateral transport into the lipid bilayer. The Sec-associated YidC appears to assist the lateral transport of IMPs from the Sec translocase into the lipid bilayer. It should be noted that targeting and translocation of only a handful of secretory proteins and IMPs have been studied. These model proteins do not include lipoproteins. Here, we have studied the targeting and translocation of two secretory lipoproteins, the murein lipoprotein and the bacteriocin release protein, using a combined in vivo and in vitro approach. The data indicate that both murein lipoprotein and bacteriocin release protein require the SRP pathway for efficient targeting to the Sec translocase. Furthermore, we show that YidC plays an important role in the targeting/translocation of both lipoproteins