Lifetimes of Confined Acoustic Phonons in Ultra-Thin Silicon Membranes

We study the relaxation of coherent acoustic phonon modes with frequencies up to 500 GHz in ultra-thin free-standing silicon membranes. Using an ultrafast pump-probe technique of asynchronous optical sampling, we observe that the decay time of the first-order dilatational mode decreases significantly from \sim 4.7 ns to 5 ps with decreasing membrane thickness from \sim 194 to 8 nm. The experimental results are compared with theories considering both intrinsic phonon-phonon interactions and extrinsic surface roughness scattering including a wavelength-dependent specularity. Our results provide insight to understand some of the limits of nanomechanical resonators and thermal transport in nanostructures

Quantitative 3w-scanning thermal microscopy : Modelling the AC/DC coupling and the sample heat conduction

A way to increase the Scanning Thermal Microscope (SThM) sensitivity in the harmonic 3w mode is to heat the probe with an AC current sufficiently high to generate a coupling between the AC and the DC signals. We detail in this paper how to properly take into account this coupling with a Wollaston-probe SThM. We also show how to link correctly the thermal conductivity to the thermal conductance measured by the SThM

Temperature Measurement of Microsystems by Scanning Thermal Microscopy

Submitted on behalf of EDA Publishing Association (http://irevues.inist.fr/handle/2042/5920)International audienceSurface temperature measurements were performed with a Scanning Thermal Microscope. We aim at proving an eventual sub-micrometric resolution of this metrology when using a wollaston wire probe of micrometric size. A dedicated CMOS device was designed with arrays of lines 0.35mm in size with 0.8 mm and 10mm periods. Integrated Circuits with or without a passivition layer were tested. To enhance sensitivity, the IC heat source was excited with an AC current. We show that the passivation layer spreads heat so that the lines are not distinguishable. Removing this layer allows us to distinguish the lines in the case of the 10mm period

Immunogenicity and safety of yellow fever vaccination for 102 HIV-infected patients

BACKGROUND: Yellow fever vaccine (17DV) has been investigated incompletely in human immunodeficiency virus (HIV)-infected patients, and adequate immunogenicity and safety are of concern in this population. METHODS: In the Swiss HIV Cohort Study, we identified 102 patients who received 17DV while they were HIV infected. We analyzed neutralization titers (NTs) after 17DV administration using the plaque reduction neutralization test. NTs of 1:>or=10 were defined as reactive, and those of 1:<10 were defined as nonreactive, which was considered to be nonprotective. The results were compared with data for HIV-uninfected individuals. Serious adverse events were defined as hospitalization or death within 6 weeks after receipt of 17DV. RESULTS: At the time of 17DV administration, the median CD4 cell count was 537 cells/mm(3) (range, 11-1730 cells/mm(3)), and the HIV RNA level was undetectable in 41 of 102 HIV-infected patients. During the first year after vaccination, fewer HIV-infected patients (65 [83%] of 78; P = .01) than HIV-uninfected patients revealed reactive NTs, and their NTs were significantly lower (P < .001) than in HIV-uninfected individuals. Eleven patients with initially reactive NTs lost these reactive NTs <or= 5 years after vaccination. Higher NTs during the first year after vaccination were associated with undetectable HIV RNA levels, increasing CD4 cell count, and female sex. We found no serious adverse events after 17DV administration among HIV-infected patients. CONCLUSION: Compared with HIV-uninfected individuals, HIV-infected patients respond to 17DV with lower reactive NTs, more often demonstrate nonprotective NTs, and may experience a more rapid decline in NTs during follow-up. Vaccination with 17DV appears to be safe in HIV-infected individuals who have high CD4 cell counts, although rate of serious adverse events of up to 3% cannot be exclude

Multiwavelength observation of a new black hole candidate: EXS 1737.9-2952

We report a multiwavelength analysis of an unusual high-energy transient: EXS 1737.9-2952. Due to the features this source exhibited in the hard X-ray domain similar to another source in the Galactic center region (1E1740.9 - 2952), and in order to study the molecular gas toward this X-ray source, we performed an observation of the EXS region in 1993 August, using the Swedish-ESO Submillimeter Telescope (SEST) located in La Silla (Chilean Andes). We observed a cloud, at the "forbidden" velocity of 135 km s-1, using 12CO (1-0) transitions, giving a maximum column density of 8 &#215; 1021 cm-2. In 1994 we conducted other observations to search for higher density regions inside the cloud, using HCO+ and CS lines, but they were unsuccessful: we concluded that the cloud could be associated with the X-ray source and its mean density is of the order of &#8776;103 cm-3. In 1994 April, we performed a set of observations of the field containing EXS, at 20 cm and 6 cm, using the VLA in its A configuration, and found four possible radio candidates for an association with the EXS X-ray source, one of them (source 3) being extended, exhibiting a head-tail morphology, and a having a thermal spectrum with a spectral index &#8776;-0.7. We reproduced our observation in 1994 November and December, using the C configuration at 6 cm, in order to investigate on possible variability and extension of these sources and found a marginal indication in the 20 cm image that source 3 may have a weak second component displaced about 15". Nevertheless, this indication is too faint to associate this source definitely with EXS since no significant variation was detected. In addition, during the 1994 November-December observation, two more extended sources were detected but their association with EXS is unlikely. We also analyzed the Einstein/IPC image of the 5 &#963; EXS error box which does not exhibit, at the time of the observation, any significant low-energy X-ray counterpart to EXS. A nearby pulsar PSR 1737-30 in the ROSAT catalog is outside this error box. Finally, IRAS maps of the EXS region do not show any IR contribution at the location of the radio sources. We conclude that (1) EXS 1737.9-2952 is a high-energy transient, (2) a persistent counterpart at other wavelengths is not demonstrated, and (3) EXS, when flaring, as well as other GC gamma-ray sources, could possibly contribute to the 511 keV bulge emission

Characterizing a new class of variability in GRS 1915+105 with simultaneous INTEGRAL/RXTE observations

We report on the analysis of 100 ks INTEGRAL observations of the Galactic microquasar GRS 1915+105. We focus on INTEGRAL Revolution number 48 when the source was found to exhibit a new type of variability as preliminarily reported in Hannikainen et al. (2003). The variability pattern, which we name $\xi$, is characterized by a pulsing behaviour, consisting of a main pulse and a shorter, softer, and smaller amplitude precursor pulse, on a timescale of 5 minutes in the JEM-X 3-35 keV lightcurve. We also present simultaneous RXTE data. From a study of the individual RXTE/PCA pulse profiles we find that the rising phase is shorter and harder than the declining phase, which is opposite to what has been observed in other otherwise similar variability classes in this source. The position in the colour-colour diagram throughout the revolution corresponds to State A (Belloni et al. 2000) but not to any previously known variability class. We separated the INTEGRAL data into two subsets covering the maxima and minima of the pulses and fitted the resulting two broadband spectra with a hybrid thermal--non-thermal Comptonization model. The fits show the source to be in a soft state characterized by a strong disc component below ~6 keV and Comptonization by both thermal and non-thermal electrons at higher energies.Comment: Accepted for publication in A&A. 11 pages, 10 figures, 4 in colour. Original figures can be found at http://www.astro.helsinki.fi/~diana/grs1915_rev48. Author affiliations correcte

A human coronavirus responsible for the common cold massively kills dendritic cells but not monocytes

Copyright @ 2012, American Society for Microbiology.Human coronaviruses are associated with upper respiratory tract infections that occasionally spread to the lungs and other organs. Although airway epithelial cells represent an important target for infection, the respiratory epithelium is also composed of an elaborate network of dendritic cells (DCs) that are essential sentinels of the immune system, sensing pathogens and presenting foreign antigens to T lymphocytes. In this report, we show that in vitro infection by human coronavirus 229E (HCoV-229E) induces massive cytopathic effects in DCs, including the formation of large syncytia and cell death within only few hours. In contrast, monocytes are much more resistant to infection and cytopathic effects despite similar expression levels of CD13, the membrane receptor for HCoV-229E. While the differentiation of monocytes into DCs in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4 requires 5 days, only 24 h are sufficient for these cytokines to sensitize monocytes to cell death and cytopathic effects when infected by HCoV-229E. Cell death induced by HCoV-229E is independent of TRAIL, FasL, tumor necrosis factor alpha, and caspase activity, indicating that viral replication is directly responsible for the observed cytopathic effects. The consequence of DC death at the early stage of HCoV-229E infection may have an impact on the early control of viral dissemination and on the establishment of long-lasting immune memory, since people can be reinfected multiple times by HCoV-229E

Virulence and Pathogen Multiplication: A Serial Passage Experiment in the Hypervirulent Bacterial Insect-Pathogen Xenorhabdus nematophila

The trade-off hypothesis proposes that the evolution of pathogens' virulence is shaped by a link between virulence and contagiousness. This link is often assumed to come from the fact that pathogens are contagious only if they can reach high parasitic load in the infected host. In this paper we present an experimental test of the hypothesis that selection on fast replication can affect virulence. In a serial passage experiment, we selected 80 lines of the bacterial insect-pathogen Xenorhabdus nematophila to multiply fast in an artificial culture medium. This selection resulted in shortened lag phase in our selected bacteria. We then injected these bacteria into insects and observed an increase in virulence. This could be taken as a sign that virulence in Xenorhabdus is linked to fast multiplication. But we found, among the selected lineages, either no link or a positive correlation between lag duration and virulence: the most virulent bacteria were the last to start multiplying. We then surveyed phenotypes that are under the control of the flhDC super regulon, which has been shown to be involved in Xenorhabdus virulence. We found that, in one treatment, the flhDC regulon has evolved rapidly, but that the changes we observed were not connected to virulence. All together, these results indicate that virulence is, in Xenorhabdus as in many other pathogens, a multifactorial trait. Being able to grow fast is one way to be virulent. But other ways exist which renders the evolution of virulence hard to predict

Effects of interspecific gene flow on the phenotypic variance–covariance matrix in Lake Victoria Cichlids

Quantitative genetics theory predicts adaptive evolution to be constrained along evolutionary lines of least resistance. In theory, hybridization and subsequent interspecific gene flow may, however, rapidly change the evolutionary constraints of a population and eventually change its evolutionary potential, but empirical evidence is still scarce. Using closely related species pairs of Lake Victoria cichlids sampled from four different islands with different levels of interspecific gene flow, we tested for potential effects of introgressive hybridization on phenotypic evolution in wild populations. We found that these effects differed among our study species. Constraints measured as the eccentricity of phenotypic variance–covariance matrices declined significantly with increasing gene flow in the less abundant species for matrices that have a diverged line of least resistance. In contrast, we find no such decline for the more abundant species. Overall our results suggest that hybridization can change the underlying phenotypic variance–covariance matrix, potentially increasing the adaptive potential of such populations