La representación de las mujeres trabajadoras en las series de máxima audiencia emitidas en España (2010)

En este artículo, presentamos un análisis de contenido realizado sobre las 16 series de más audiencia emitidas en España y producidas en idioma español, durante el año 2010. En total se han analizado 143 personajes masculinos y 144 femeninos. Veremos que los primeros resultados del análisis nos muestran que no existe una equiparación de género en las representaciones de estas series. El dominio del espacio público sigue perteneciendo a los personajes masculinos y las mujeres siguen ocupando en muchos casos profesiones menos prestigiosas y tradicionalmente femeninas, centradas en el cuidado del prójimo y al servicio de los demás.In this article we present a content analysis conducted on the 16 highest-rated tv series broadcasted in Spain and produced in Spanish language in 2010. We have analyzed a total of 143 male and 144 female characters. We present the first results of the analysis demonstrating that there is no gender equality in the representations of these series. The dominance in the public space still belongs to the male characters and women are still often occupying less prestigious and traditionally female-centered professions related to serving and taking care of others

Análisis de la ficción iberoamericana de mayor audiencia en el mercado español

Este artículo analiza las características de las series de ficción de producción iberoamericana con más audiencia en España durante el año 2010 con el objetivo de determinar las preferencias de la audiencia de cara a los programadores y establecer pautas de producción con una perspectiva más diversa y educativa. Se presenta un análisis de audiencia y un estudio del perfil social y demográfico de los personajes de dichas series. Las conclusiones demuestran la necesidad de introducir una mayor diversidad en la construcción de los personajes para atender a un telespectador que demanda un producto de ficción más representativo respecto a la sociedad actual.Artikulu honek aztergai ditu Espainian 2010 urtean zehar audientzia handiena izan zuten produkzio iberoamerikarreko fikziozko telesailak. Helburua audientziaren zaletasunak zehaztea da, honela programatzaileek datu horiek erabili eta produkziojarraibideak ikuspuntu ezberdin eta hezitzaileagoan ezartzeko. Telesail horien audientzia-analisia eta fikzio horietan agertzen diren pertsonaien profil sozial eta demografikoaren azterketa aurkezten da. Ondorioek erakusten dute komenigarria litzatekeela pertsonaien eraketan aniztasun handiagoa egotea, ikus-entzuleak gaur egungo gizartea hobeto islatzen duten fikzio-produktuak eskatzen baititu.This article analyses the characteristics of the Ibero-American most watched TV series in Spain in 2010 with the aim of define the viewer's preferences and to establish guidelines for the producers that take into account a more diverse and educational perspective. We present an audience analysis, as well as, a content analysis of the demographic and social roles of the characters in these TV series. The article reflects on the need for greater diversity in the construction of the characters in order to satisfy viewers' expectations

The effects of irrigation modernization on the cost recovery of water in the Valencia Region (Spain)

The modernization of the irrigation systems has been the main strategy followed by the regional administration of the Valencia Region to cope with the structural water deficit of the region, which has been particularly severe during the last three decades. These policies have been oriented to the substitution of gravity irrigation systems for drip technology. The technological change has involved an important investment effort, developed by different public administrations and also the farmers and water users associations (WUAs). This transformation, has also involved a change in the structure of costs of the WUAs. This paper analyzes the changes in costs and tariffs of irrigation after the important investments made in the modernization of irrigation. The effects of subsidies on the percentage of the cost recovery in the services of water for irrigation are also considered. All of them have developed modernization projects in the recent years. It can be concluded that conflict between two objectives proposed by the Water Framework Directive may exist. On the one hand, significant reductions of water supply are observed; meanwhile, on the other hand, the cost recovery percentage diminishes significantly.García Molla, M.; Ortega Reig, MV.; Sanchis Ibor, C.; Avella Reus, LF. (2014). The effects of irrigation modernization on the cost recovery of water in the Valencia Region (Spain). Water Science and Technology: Water Supply. 14(3):414-420. doi:10.2166/ws.2013.215S41442014

Aplicación del programa "Mathematica" a las prácticas de cálculo en el primer año universitario

En este trabajo se describe una investigación acerca de las prácticas de Cálculo realizadas por estudiantes universitarios de primer curso en entornos informáticos en los que se utiliza el programa MATHEMATICA. Se analizan los errores y dificultades que aparecen en las contestaciones a las cuestiones planteadas en una prueba de evaluación. Todo esto dentro del marco teórico del enfoque ontosemiótico de la cognición matemática (Godino, 2002). Los análisis indican que el uso de dicho entorno informático no garantiza unos resultados satisfactorios, en cuanto a la enseñanza-aprendizaje de los conceptos de límite, continuidad y derivada de una función

Gene Expression Patterns Distinguish Mortality Risk in Patients with Postsurgical Shock

Producción CientíficaNowadays, mortality rates in intensive care units are the highest of all hospital units. However, there is not a reliable prognostic system to predict the likelihood of death in patients with postsurgical shock. Thus, the aim of the present work is to obtain a gene expression signature to distinguish the low and high risk of death in postsurgical shock patients. In this sense, mRNA levels were evaluated by microarray on a discovery cohort to select the most differentially expressed genes between surviving and non-surviving groups 30 days after the operation. Selected genes were evaluated by quantitative real-time polymerase chain reaction (qPCR) in a validation cohort to validate the reliability of data. A receiver-operating characteristic analysis with the area under the curve was performed to quantify the sensitivity and specificity for gene expression levels, which were compared with predictions by established risk scales, such as acute physiology and chronic health evaluation (APACHE) and sequential organ failure assessment (SOFA). IL1R2, CD177, RETN, and OLFM4 genes were upregulated in the non-surviving group of the discovery cohort, and their predictive power was confirmed in the validation cohort. This work offers new biomarkers based on transcriptional patterns to classify the postsurgical shock patients according to low and high risk of death. The results present more accuracy than other mortality risk scores.Instituto de Salud Carlos III (grant PI15/01451)Junta de Castilla y León (grant 1255/A/16)Universidad de Valladolid - Fondo Europeo de Desarrollo Regional (grant VA321P18

Enhanced mitochondrial activity reshapes a gut microbiota profile that delays NASH progression

[EN] Background and Aims: Recent studies suggest that mitochondrial dysfunction promotes progression to NASH by aggravating the gut-liver status. However, the underlying mechanism remains unclear. Herein, we hypothesized that enhanced mitochondrial activity might reshape a specific microbiota signature that, when transferred to germ-free (GF) mice, could delay NASH progression. Approach and Results: Wild-type and methylation-controlled J protein knockout (MCJ-KO) mice were fed for 6 weeks with either control or a choline-deficient, L-amino acid–defined, high-fat diet (CDA-HFD). One mouse of each group acted as a donor of cecal microbiota to GF mice, who also underwent the CDA-HFD model for 3 weeks. Hepatic injury, intestinal barrier, gut microbiome, and the associated fecal metabolome were then studied. Following 6 weeks of CDA-HFD, the absence of methylation-controlled J protein, an inhibitor of mitochondrial complex I activity, reduced hepatic injury and improved gut-liver axis in an aggressive NASH dietary model. This effect was transferred to GF mice through cecal microbiota transplantation. We suggest that the specific microbiota profile of MCJ-KO, characterized by an increase in the fecal relative abundance of Dorea and Oscillospira genera and a reduction in AF12, Allboaculum, and [Ruminococcus], exerted protective actions through enhancing short-chain fatty acids, nicotinamide adenine dinucleotide (NAD+) metabolism, and sirtuin activity, subsequently increasing fatty acid oxidation in GF mice. Importantly, we identified Dorea genus as one of the main modulators of this microbiota-dependent protective phenotype. Conclusions: Overall, we provide evidence for the relevance of mitochondria–microbiota interplay during NASH and that targeting it could be a valuable therapeutic approach.S

Genome-wide whole blood transcriptome profiling in a large European cohort of systemic sclerosis patients

Objectives The analysis of annotated transcripts from genome-wide expression studies may help to understand the pathogenesis of complex diseases, such as systemic sclerosis (SSc). We performed a whole blood (WB) transcriptome analysis on RNA collected in the context of the European PRECISESADS project, aiming at characterising the pathways that differentiate SSc from controls and that are reproducible in geographically diverse populations. Methods Samples from 162 patients and 252 controls were collected in RNA stabilisers. Cases and controls were divided into a discovery (n=79+163; Southern Europe) and validation cohort (n=83+89; Central-Western Europe). RNA sequencing was performed by an Illumina assay. Functional annotations of Reactome pathways were performed with the Functional Analysis of Individual Microarray Expression (FAIME) algorithm. In parallel, immunophenotyping of 28 circulating cell populations was performed. We tested the presence of differentially expressed genes/pathways and the correlation between absolute cell counts and RNA transcripts/FAIME scores in regression models. Results significant in both populations were considered as replicated. Results Overall, 15 224 genes and 1277 functional pathways were available; of these, 99 and 225 were significant in both sets. Among replicated pathways, we found a deregulation in type-I interferon, Toll-like receptor cascade, tumour suppressor p53 protein function, platelet degranulation and activation. RNA transcripts or FAIME scores were jointly correlated with cell subtypes with strong geographical differences; neutrophils were the major determinant of gene expression in SSc-WB samples. Conclusions We discovered a set of differentially expressed genes/pathways validated in two independent sets of patients with SSc, highlighting a number of deregulated processes that have relevance for the pathogenesis of autoimmunity and SSc.EU/EFPIA/Innovative Medicines Initiative Joint Undertaking PRECISESADS 115 56

Endothelial dysfunction is an early indicator of sepsis and neutrophil degranulation of septic shock in surgical patients

Producción CientíficaBackground: Stratification of the severity of infection is currently based on the Sequential Organ Failure Assessment (SOFA) score, which is difficult to calculate outside the ICU. Biomarkers could help to stratify the severity of infection in surgical patients. Methods: Levels of ten biomarkers indicating endothelial dysfunction, 22 indicating emergency granulopoiesis, and six denoting neutrophil degranulation were compared in three groups of patients in the first 12 h after diagnosis at three Spanish hospitals. Results: There were 100 patients with infection, 95 with sepsis and 57 with septic shock. Seven biomarkers indicating endothelial dysfunction (mid-regional proadrenomedullin (MR-ProADM), syndecan 1, thrombomodulin, angiopoietin 2, endothelial cell-specific molecule 1, vascular cell adhesion molecule 1 and E-selectin) had stronger associations with sepsis than infection alone. MR-ProADM had the highest odds ratio (OR) in multivariable analysis (OR 11·53, 95 per cent c.i. 4·15 to 32·08; P = 0·006) and the best area under the curve (AUC) for detecting sepsis (0·86, 95 per cent c.i. 0·80 to 0·91; P < 0·001). In a comparison of sepsis with septic shock, two biomarkers of neutrophil degranulation, proteinase 3 (OR 8·09, 1·34 to 48·91; P = 0·028) and lipocalin 2 (OR 6·62, 2·47 to 17·77; P = 0·002), had the strongest association with septic shock, but lipocalin 2 exhibited the highest AUC (0·81, 0·73 to 0·90; P < 0·001). Conclusion: MR-ProADM and lipocalin 2 could be alternatives to the SOFA score in the detection of sepsis and septic shock respectively in surgical patients with infection.Instituto de Salud Carlos III (grants PI15/01959, PI15/01451 and PI16/01156

The challenge of the digital divide and elderly in Spanish rural areas. The case of Castilla y León.

This article includes some reflections about the tandem between new technologies, particularly the Internet, and elderly people living in rural areas. This article combines a qualitative (personal interviews and focus groups) andquantitative methodology (analysis of demographic data), aiming to deep about the relationship between relationshipbetween the digital divide and geographical gap for elderly people living in rural areas in Castilla y León. Results obtained from this research show the existence of structural elements in these rural areas but also symbolic aspects often overlooked, which must take into account when implementing intervention projects. This study confirms theneed to attend to the territory as a variable but also the characteristics of those who have greater difficulties in useand access to ICT, and progress towards equal opportunities.El presente artículo versa sobre el binomio Nuevas Tecnologías (en especial Internet) y las personas mayores que habitan en áreas rurales. A partir de una metodología tanto cualitativa (entrevistas personales y grupos de discusión) como cuantitativa (análisis de datos demográficos), se pretende profundizar en la relación entre la brecha digital y la brecha geográfica para las personas mayores residentes en el medio rural de Castilla y León. Los resultados obtenidos muestran la existencia de elementos estructurales diferenciados en estas zonas rurales, así como otros aspectos simbólicos con frecuencia olvidados, que han de ser tenidos en cuenta a la hora de implementar proyectos de intervención. El trabajo confirma la necesidad de atender al territorio como variable, pero también a las características de unos destinatarios que tienen mayores dificultades de uso y acceso a las TIC, y avanzar así en pro de la igualdad de oportunidades

The outcome of boosting mitochondrial activity in alcohol-associated liver disease is organ-dependent.

BACKGROUND AND AIMS Alcohol-associated liver disease (ALD) accounts for 70% of liver-related deaths in Europe, with no effective approved therapies. Although mitochondrial dysfunction is one of the earliest manifestations of alcohol-induced injury, restoring mitochondrial activity remains a problematic strategy due to oxidative stress. Here, we identify methylation-controlled J protein (MCJ) as a mediator for ALD progression and hypothesize that targeting MCJ may help in recovering mitochondrial fitness without collateral oxidative damage. APPROACH AND RESULTS C57BL/6 mice [wild-type (Wt)] Mcj knockout and Mcj liver-specific silencing (MCJ-LSS) underwent the NIAAA dietary protocol (Lieber-DeCarli diet containing 5% (vol/vol) ethanol for 10 days, plus a single binge ethanol feeding at day 11). To evaluate the impact of a restored mitochondrial activity in ALD, the liver, gut, and pancreas were characterized, focusing on lipid metabolism, glucose homeostasis, intestinal permeability, and microbiota composition. MCJ, a protein acting as an endogenous negative regulator of mitochondrial respiration, is downregulated in the early stages of ALD and increases with the severity of the disease. Whole-body deficiency of MCJ is detrimental during ALD because it exacerbates the systemic effects of alcohol abuse through altered intestinal permeability, increased endotoxemia, and dysregulation of pancreatic function, which overall worsens liver injury. On the other hand, liver-specific Mcj silencing prevents main ALD hallmarks, that is, mitochondrial dysfunction, steatosis, inflammation, and oxidative stress, as it restores the NAD + /NADH ratio and SIRT1 function, hence preventing de novo lipogenesis and improving lipid oxidation. CONCLUSIONS Improving mitochondrial respiration by liver-specific Mcj silencing might become a novel therapeutic approach for treating ALD.This work was supported by grants from Ministerio de Ciencia e Innovación, Programa Retos-Colaboración RTC2019-007125-1 (for Jorge Simon and Maria Luz Martinez-Chantar); Ministerio de Economía, Industria y Competitividad, Retos a la Sociedad AGL2017- 86927R (for F.M.); Instituto de Salud Carlos III, Proyectos de Investigación en Salud DTS20/00138 and DTS21/00094 (for Jorge Simon and Maria Luz Martinez-Chantar, and Asis Palazon. respectively); Instituto de Salud Carlos III, Fondo de Investigaciones Sanitarias co-founded by European Regional Development Fund/European Social Fund, “Investing in your future” PI19/00819, “Una manera de hacer Europa” FIS PI20/00765, and PI21/01067 (for Jose J. G. Marin., Pau Sancho-Bru,. and Mario F. Fraga respectively); Departamento de Industria del Gobierno Vasco (for Maria Luz Martinez-Chantar); Asturias Government (PCTI) co-funding 2018-2023/ FEDER IDI/2021/000077 (for Mario F. Fraga.); Ministerio de Ciencia, Innovación y Universidades MICINN: PID2020-117116RB-I00, CEX2021-001136-S PID2020-117941RB-I00, PID2020-11827RB-I00 and PID2019-107956RA-100 integrado en el Plan Estatal de Investigación Científica y Técnica y Innovación, cofinanciado con Fondos FEDER (for Maria Luz Martinez-Chantar, Francisco J Cubero., Yulia A Nevzorova and Asis Palazon); Ayudas Ramón y Cajal de la Agencia Estatal de Investigación RY2013-13666 and RYC2018- 024183-I (for Leticia Abecia and Asis Palazon); European Research Council Starting Grant 804236 NEXTGEN-IO (for Asis Palazon); The German Research Foundation SFB/TRR57/P04, SFB1382-403224013/ A02 and DFG NE 2128/2-1 (for Francisco J Cubero and Yulia A Nevzorova); National Institute of Health (NIH)/National Institute of Alcohol Abuse and Alcoholism (NIAAA) 1U01AA026972-01 (For Pau Sancho-Bru); Junta de Castilla y León SA074P20 (for Jose J. G. Marin); Junta de Andalucía, Grupo PAIDI BIO311 (for Franz Martin); CIBERER Acciones Cooperativas y Complementarias Intramurales ACCI20-35 (for Mario F. Fraga); Ministerio de Educación, Cultura y Deporte FPU17/04992 (for Silvia Ariño); Fundació Marato TV3 201916-31 (for Jose J. G. Marin.); Ainize Pena-Cearra is a fellow of the University of the Basque Country (UPV/ EHU); BIOEF (Basque Foundation for Innovation and Health Research); Asociación Española contra el Cáncer (Maria Luz Martinez-Chantar and Teresa C. Delgado.); Fundación Científica de la Asociación Española Contra el Cáncer (AECC Scientific Foundation) Rare Tumor Calls 2017 (for Maria Luz Martinez-Chantar); La Caixa Foundation Program (for Maria Luz Martinez-Chantar); Proyecto Desarrollo Tecnologico CIBERehd (for Maria Luz Martinez-Chantar); Ciberehd_ISCIII_MINECO is funded by the Instituto de Salud Carlos III.S