Maternal prenatal depression is associated with decreased placental expression of the imprinted gene PEG3.

BACKGROUND: Maternal prenatal stress during pregnancy is associated with fetal growth restriction and adverse neurodevelopmental outcomes, which may be mediated by impaired placental function. Imprinted genes control fetal growth, placental development, adult behaviour (including maternal behaviour) and placental lactogen production. This study examined whether maternal prenatal depression was associated with aberrant placental expression of the imprinted genes paternally expressed gene 3 (PEG3), paternally expressed gene 10 (PEG10), pleckstrin homology-like domain family a member 2 (PHLDA2) and cyclin-dependent kinase inhibitor 1C (CDKN1C), and resulting impaired placental human placental lactogen (hPL) expression. METHOD: A diagnosis of depression during pregnancy was recorded from Manchester cohort participants' medical notes (n = 75). Queen Charlotte's (n = 40) and My Baby and Me study (MBAM) (n = 81) cohort participants completed the Edinburgh Postnatal Depression Scale self-rating psychometric questionnaire. Villous trophoblast tissue samples were analysed for gene expression. RESULTS: In a pilot study, diagnosed depression during pregnancy was associated with a significant reduction in placental PEG3 expression (41%, p = 0.02). In two further independent cohorts, the Queen Charlotte's and MBAM cohorts, placental PEG3 expression was also inversely associated with maternal depression scores, an association that was significant in male but not female placentas. Finally, hPL expression was significantly decreased in women with clinically diagnosed depression (44%, p < 0.05) and in those with high depression scores (31% and 21%, respectively). CONCLUSIONS: This study provides the first evidence that maternal prenatal depression is associated with changes in the placental expression of PEG3, co-incident with decreased expression of hPL. This aberrant placental gene expression could provide a possible mechanistic explanation for the co-occurrence of maternal depression, fetal growth restriction, impaired maternal behaviour and poorer offspring outcomes.The Manchester cohort was supported by Manchester National Institute for Health Research (NIHR) Biomedical Research. The Queen Charlotte’s cohort was supported by the Medical Research Council (MRC) (Eurostress), National Institutes of Health (R01MH073842) and the Genesis Research Trust. The MBAM cohort was supported by the Genesis Research Trust. A.B.J. was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) Doctoral Training Grants (DTG) studentship and subsequently MRC project grant MR/M013960/1. S.J.T. was supported by BBSRC project grant BB/J015156/1. L.E.C. was supported by an Imperial College London Ph.D. studentship and both L.E.C. and P.G.R were supported by the NIHR Imperial Biomedical Research Centre

Understanding the nature of Ds(2317)D_s(2317) and Ds(2460)D_s(2460) through nonleptonic B Decays

We consider the nonleptonic B decays $B \to D^{(*)} D_s(2317)$ and $B \to D^{(*)} D_s(2460)$, involving the newly discovered $D_s(2317)$ and the $D_s(2460)$ states. We find that experiments indicate disagreement with model calculations of their properties and/or breakdown of the factorization assumption for these decays . We point out that decays involving $B_s$ mesons where the $D_s$ resonances can be produced via the weak decay of the $b$ quark can provide further information about the nature of these newly discovered states. We also propose a model to calculate the two body nonleptonic decays $B \to D^{(*)} D_s(2317)(D_s(2460))$, if the $D_s(2317)$ and $D_s(2460)$ are interpreted as $DK$ and $D^*K$ molecules.Comment: 14 pages LaTeX; additional reference, notational corrections and minor clarifications of tex

Helping behaviour during cooperative learning and learning gains

Is helping behaviour (i.e., solicited help and peer tutoring) during cooperative learning (CL) related to subsequent learning gains? And can teachers influence pupils’ helping behaviour? One hundred one 5th grade pupils from multiethnic schools, 10-12 years old, participated in the study. Forty two pupils (31 immigrant) worked in an experimental condition, characterized by the stimulation of solicited high quality help and 59 (24 immigrant) worked in a control condition. It was found that learning gains were predicted positively by pupils’ unsolicited helping behaviour (i.e., peer tutoring) and negatively by solicited help. Furthermore, teachers were able to affect pupils’ low quality solicited help only. Lastly, immigrant pupils used less helping behaviour than local pupils, irrespective of CL setting

A Novel Deep Clustering Framework for Fine-Scale Parcellation of Amygdala Using dMRI Tractography

The amygdala plays a vital role in emotional processing and exhibits structural diversity that necessitates fine-scale parcellation for a comprehensive understanding of its anatomico-functional correlations. Diffusion MRI tractography is an advanced imaging technique that can estimate the brain's white matter structural connectivity to potentially reveal the topography of the amygdala for studying its subdivisions. In this work, we present a deep clustering pipeline to perform automated, fine-scale parcellation of the amygdala using diffusion MRI tractography. First, we incorporate a newly proposed deep learning approach to enable accurate segmentation of the amygdala directly on the dMRI data. Next, we design a novel streamline clustering-based structural connectivity feature for a robust representation of voxels within the amygdala. Finally, we improve the popular joint dimensionality reduction and k-means clustering approach to enable amygdala parcellation at a finer scale. With the proposed method, we obtain nine unique amygdala parcels. Experiments show that these parcels can be consistently identified across subjects and have good correspondence to the widely used coarse-scale amygdala parcellation

On the measurement of horizontal inequity in the delivery of health care

This paper offers a critical appraisal of the various methods used to date to investigate inequity in the delivery of health care. It concludes that none of the methods used to date is particularly well equipped to provide unbiassed estimates of the extent of inequity. It also concludes that Le Grand's (1978) approach is likely to point towards inequity favouring the rich even when none exists. The paper offers an alternative approach, which builds on the approaches to date but seeks to overcome their deficiencies

First identification and characterization of Borrobol-type tephra in the Greenland ice cores::new deposits and improved age estimates

Contiguous sampling of ice spanning key intervals of the deglaciation from the Greenland ice cores of NGRIP, GRIP and NEEM has revealed three new silicic cryptotephra deposits that are geochemically similar to the well-known Borrobol Tephra (BT). The BT is complex and confounded by the younger closely timed and compositionally similar Penifiler Tephra (PT). Two of the deposits found in the ice are in Greenland Interstadial 1e (GI-1e) and an older deposit is found in Greenland Stadial 2.1 (GS-2.1). Until now, the BT was confined to GI-1-equivalent lacustrine sequences in the British Isles, Sweden and Germany, and our discovery in Greenland ice extends its distribution and geochemical composition. However, the two cryptotephras that fall within GI-1e ice cannot be separated on the basis of geochemistry and are dated to 14358 ± 177 a b2k and 14252 ± 173 a b2k, just 106 ± 3 years apart. The older deposit is consistent with BT age estimates derived from Scottish sites, while the younger deposit overlaps with both BT and PT age estimates. We suggest that either the BT in Northern European terrestrial sequences represents an amalgamation of tephra from both of the GI-1e events identified in the ice-cores or that it relates to just one of the ice-core events. A firm correlation cannot be established at present due to their strong geochemical similarities. The older tephra horizon, found within all three ice-cores and dated to 17326 ± 319 a b2k, can be correlated to a known layer within marine sediment cores from the North Iceland Shelf (ca. 17179-16754 cal a BP). Despite showing similarities to the BT, this deposit can be distinguished on the basis of lower CaO and TiO2 and is a valuable new tie-point that could eventually be used in high-resolution marine records to compare the climate signals from the ocean and atmosphere

Ultraluminous X-ray sources out to z~0.3 in the COSMOS field

Using Chandra observations we have identified a sample of seven off-nuclear X-ray sources, in the redshift range z=0.072-0.283, located within optically bright galaxies in the COSMOS Survey. Using the multi-wavelength coverage available in the COSMOS field, we study the properties of the host galaxies of these ULXs. In detail, we derived their star formation rate from H_alpha measurements and their stellar masses using SED fitting techniques with the aim to compute the probability to have an off-nuclear source based on the host galaxy properties. We divide the host galaxies in different morphological classes using the available ACS/HST imaging. We find that our ULXs candidates are located in regions of the SFR versus M$_star$ plane where one or more off-nuclear detectable sources are expected. From a morphological analysis of the ACS imaging and the use of rest-frame colours, we find that our ULXs are hosted both in late and early type galaxies. Finally, we find that the fraction of galaxies hosting a ULX ranges from ~0.5% to ~0.2% going from L[0.5-2 keV]=3 x 10^39 erg s^-1 to L[0.5-2 keV]= 2 x 10^40 erg s^-1.Comment: 10 pages, 9 figures, accepted for publication in Astronomy & Astrophysic

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti.

The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachia-infected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbB-infected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential

EC-Earth3-AerChem: A global climate model with interactive aerosols and atmospheric chemistry participating in CMIP6

This paper documents the global climate model EC-Earth3-AerChem, one of the members of the EC-Earth3 family of models participating in the Coupled Model Intercomparison Project Phase 6 (CMIP6). EC-Earth3-AerChem has interactive aerosols and atmospheric chemistry and contributes to the Aerosols and Chemistry Model Intercomparison Project (AerChemMIP). In this paper, we give an overview of the model, describe in detail how it differs from the other EC-Earth3 configurations, and outline the new features compared with the previously documented version of the model (EC-Earth 2.4). We explain how the model was tuned and spun up under preindustrial conditions and characterize the model's general performance on the basis of a selection of coupled simulations conducted for CMIP6. The net energy imbalance at the top of the atmosphere in the preindustrial control simulation is on average -0.09 W m-2 with a standard deviation due to interannual variability of 0.25 W m-2, showing no significant drift. The global surface air temperature in the simulation is on average 14.08 ∼ C with an interannual standard deviation of 0.17 ∼ C, exhibiting a small drift of 0.015 ± 0.005 ∼ C per century. The model's effective equilibrium climate sensitivity is estimated at 3.9 ∼ C, and its transient climate response is estimated at 2.1 ∼ C. The CMIP6 historical simulation displays spurious interdecadal variability in Northern Hemisphere temperatures, resulting in a large spread across ensemble members and a tendency to underestimate observed annual surface temperature anomalies from the early 20th century onwards. The observed warming of the Southern Hemisphere is well reproduced by the model. Compared with the ECMWF (European Centre for Medium-Range Weather Forecasts) Reanalysis version 5 (ERA5), the surface air temperature climatology for 1995-2014 has an average bias of -0.86 ± 0.05 ∼ C with a standard deviation across ensemble members of 0.35 ∼ C in the Northern Hemisphere and 1.29 ± 0.02 ∼ C with a corresponding standard deviation of 0.05 ∼ C in the Southern Hemisphere. The Southern Hemisphere warm bias is largely caused by errors in shortwave cloud radiative effects over the Southern Ocean, a deficiency of many climate models. Changes in the emissions of near-term climate forcers (NTCFs) have significant effects on the global climate from the second half of the 20th century onwards. For the SSP3-7.0 Shared Socioeconomic Pathway, the model gives a global warming at the end of the 21st century (2091-2100) of 4.9 ∼ C above the preindustrial mean. A 0.5 ∼ C stronger warming is obtained for the AerChemMIP scenario with reduced emissions of NTCFs. With concurrent reductions of future methane concentrations, the warming is projected to be reduced by 0.5 ∼ C

EC-Earth3-AerChem : a global climate model with interactive aerosols and atmospheric chemistry participating in CMIP6

This paper documents the global climate model EC-Earth3-AerChem, one of the members of the EC-Earth3 family of models participating in the Coupled Model Intercomparison Project Phase 6 (CMIP6). EC-Earth3-AerChem has interactive aerosols and atmospheric chemistry and contributes to the Aerosols and Chemistry Model Intercomparison Project (AerChemMIP). In this paper, we give an overview of the model, describe in detail how it differs from the other EC-Earth3 configurations, and outline the new features compared with the previously documented version of the model (EC-Earth 2.4). We explain how the model was tuned and spun up under preindustrial conditions and characterize the model's general performance on the basis of a selection of coupled simulations conducted for CMIP6. The net energy imbalance at the top of the atmosphere in the preindustrial control simulation is on average 0.09 Wm(-2) with a standard deviation due to interannual variability of 0.25 Wm(-2), showing no significant drift. The global surface air temperature in the simulation is on average 14.08 degrees C with an interannual standard deviation of 0.17 degrees C, exhibiting a small drift of 0.015 +/- 0.005 degrees C per century. The model's effective equilibrium climate sensitivity is estimated at 3.9 degrees C, and its transient climate response is estimated at 2.1 degrees C. The CMIP6 historical simulation displays spurious interdecadal variability in Northern Hemisphere temperatures, resulting in a large spread across ensemble members and a tendency to underestimate observed annual surface temperature anomalies from the early 20th century onwards. The observed warming of the Southern Hemisphere is well reproduced by the model. Compared with the ECMWF (European Centre for Medium-Range Weather Forecasts) Reanalysis version 5 (ERA5), the surface air temperature climatology for 1995-2014 has an average bias of -0.86 +/- 0.05 degrees C with a standard deviation across ensemble members of 0.35 degrees C in the North-ern Hemisphere and 1.29 +/- 0.02 degrees C with a corresponding standard deviation of 0.05 degrees C in the Southern Hemisphere. The Southern Hemisphere warm bias is largely caused by errors in shortwave cloud radiative effects over the Southern Ocean, a deficiency of many climate models. Changes in the emissions of near-term climate forcers (NTCFs) have significant effects on the global climate from the second half of the 20th century onwards. For the SSP3-7.0 Shared Socioeconomic Pathway, the model gives a global warming at the end of the 21st century (2091-2100) of 4.9 degrees C above the preindustrial mean. A 0.5 degrees C stronger warming is obtained for the AerChemMIP scenario with reduced emissions of NTCFs. With concurrent reductions of future methane concentrations, the warming is projected to be reduced by 0.5 degrees C.Peer reviewe