Recombinant Pseudomonas Vaccine: Technological Aspects of Obtaining and Evaluating Quality Indicators

For an aim to prevent Pseudomonas aeruginosa, a candidate recombinant vaccine has been developed. This vaccine – (RPV) was based on two protective proteins of P. aeruginosa: the outer membrane protein F (OprF) and the recombinant truncated form of the Exotoxin A (toxoid) that were adsorbed on the aluminum hydroxide. The optimal immunization schedule for mice included two intraperitoneal administrations with a two-week interval. RPV promoted to increase survival rates in challenged immunized mice and stimulated humoral and innate immune responses. During preclinical studies, we confirmed the immunogenicity of the vaccine that had not pyrogenicity, acute and chronic toxicity, allergenicity and immunotoxicity. Keywords: Pseudomonas aeruginosa, outer membrane protein F (OprF), toxoid, Pseudomonas Recombinant Vaccine (PRV

Photosynthetic Antenna Size Regulation as an Essential Mechanism of Higher Plants Acclimation to Biotic and Abiotic Factors: The Role of the Chloroplast Plastoquinone Pool and Hydrogen Peroxide

The present chapter describes the mechanisms of reactive oxygen species formation in photosynthetic reactions and the functional significance of reactive oxygen species as signal messengers in photosynthetic cells of plants. Attention is given to the acclimation mechanisms of higher plants to abiotic and biotic factors such as increased light, drought, soil salinity and colonization of plants by rhizosphere microorganisms. Special attention is paid to the reactions of reactive oxygen species with the components of the chloroplasts plastoquinone pool leading to production of hydrogen peroxide as a signal molecule, which is involved in acclimation of plants to these stress conditions. The chapter also presents the data demonstrating that regulation of the size of the light-harvesting antenna of photosystem II is one of the universal mechanisms of the structural and functional reorganization of the photosynthetic apparatus of higher plants exposed to the abiotic and biotic factors. These data were obtained for both model Arabidopsis (Arabidopsis thaliana) plants as well as for agricultural barley (Hordeum vulgare) plants. It is hypothesized that hydrogen peroxide, produced with involvement of the plastoquinone pool components, plays the role of a signaling molecule for regulation of the photosystem II antenna size in higher plants when environmental conditions change

Vasoprotective effect of effective lipid-lowering therapy in patients with <i>ST</i>-segment elevation myocardial infarction

Aim. To study the vasoprotective effects of atorvastatin depending on the achievement of the target level of low-density lipoprotein cholesterol (LDL-C) in patients with ST-segment elevation myocardial infarction (STEMI) within 48 weeks of follow-up. Materials and methods. Included were 112 STEMI patients who received atorvastatin 204080 mg. On days 79 from the onset of the disease, after 24 and 48 weeks, ultrasound examination of the carotid arteries with RF technology and applanation tonometry were performed, the lipid profile was determined. The patients were divided into groups: group 1 (n=41) of highly effective therapy (HET) who achieved the target LDL-C after 24 and 48 weeks; group 2 (n=29) in relatively effective therapy (RET) achieving target values at 24th or 48th week; group 3 (n=42) insufficiently effective therapy (IET) did not reach the target LDL-C. Results. When examining the carotid arteries in the HET group, the intima-media thickness (IMT) decreased by 10.713.1%, the b index by 14.926.3% after 2448 weeks. In the RET group, the IMT regression was 10.413.3%; b index 23.9% by the 48th week. In the IET group, the b index decreased by the 48th week by 14.3%. According to applanation tonometry in the HET group, the central pressure did not change. In the RET group, systolic pressure in the aorta increased by 1015.7% after 2448 weeks, pulse pressure by 33.9% by the end of observation. With IET, the increase was 8.66.8 and 19.825.9%, respectively. The odds ratio of developing endpoints in the RET group was 4.7 (95% CI 1.226.4; p=0.02), in the IET group 3.9 (95% CI 1.124.8; p=0.03) compared with HET. Conclusion. The most pronounced vasoprotective effect and a decrease in cardiovascular risk are associated with the achievement of the target LDL-C throughout the entire treatment period

The rs738409 (I148M) Variant of the PNPLA3 Gene and Type 2 Diabetes in Yakutia

The purpose of our research was to study the association of the PNPLA3 SNP rs738409 (C>G) with type 2 diabetes (T2D) in the Yakuts. The frequency distribution of alleles and genotypes of the PNPLA3 rs738409 SNP was in accordance with HWE. There were no statistically significant differences in the distribution of alleles and genotypes of the PNPLA3 SNP rs738409 (C>G) between T2D patients and non-T2D patients (P>0.05); the G allele and homozygous GG genotype prevailed in both groups. In T2D patients, a high frequency of the G allele (74.1%) was found, with a predominance of the GG genotype (58.5%). We also found that the mutant allele frequency is higher than in the studied populations of the world. Further studies with larger sample size are required to achieve sufficient statistical power to detect the association of the PNPLA3 SNP (rs738409 with the development of T2D in Yakut patients

The Eurasian Modern Pollen Database (EMPD), version 2

The Eurasian (née European) Modern Pollen Database (EMPD) was established in 2013 to provide a public database of high-quality modern pollen surface samples to help support studies of past climate, land cover, and land use using fossil pollen. The EMPD is part of, and complementary to, the European Pollen Database (EPD) which contains data on fossil pollen found in Late Quaternary sedimentary archives throughout the Eurasian region. The EPD is in turn part of the rapidly growing Neotoma database, which is now the primary home for global palaeoecological data. This paper describes version 2 of the EMPD in which the number of samples held in the database has been increased by 60 % from 4826 to 8134. Much of the improvement in data coverage has come from northern Asia, and the database has consequently been renamed the Eurasian Modern Pollen Database to reflect this geographical enlargement. The EMPD can be viewed online using a dedicated map-based viewer at https://empd2.github.io and downloaded in a variety of file formats at https://doi.pangaea.de/10.1594/PANGAEA.909130 (Chevalier et al., 2019)Swiss National Science Foundation | Ref. 200021_16959

The Eurasian Modern Pollen Database (EMPD), version 2

The Eurasian (nee European) Modern Pollen Database (EMPD) was established in 2013 to provide a public database of high-quality modern pollen surface samples to help support studies of past climate, land cover, and land use using fossil pollen. The EMPD is part of, and complementary to, the European Pollen Database (EPD) which contains data on fossil pollen found in Late Quaternary sedimentary archives throughout the Eurasian region. The EPD is in turn part of the rapidly growing Neotoma database, which is now the primary home for global palaeoecological data. This paper describes version 2 of the EMPD in which the number of samples held in the database has been increased by 60% from 4826 to 8134. Much of the improvement in data coverage has come from northern Asia, and the database has consequently been renamed the Eurasian Modern Pollen Database to reflect this geographical enlargement. The EMPD can be viewed online using a dedicated map-based viewer at https://empd2.github.io and downloaded in a variety of file formats at https://doi.pangaea.de/10.1594/PANGAEA.909130 (Chevalier et al., 2019).Peer reviewe

Risk Analysis and Producer Level Impact of the SAS-based

Using producer level field trials, collected over three-year period from nine farms in Florida, we conduct profitability and risk analysis of the new fungi management system under a rand of weather conditions and price levels. We utilize stochastic and NPV methodologies and find that the new system increases both profits and risk for a typical farmer in Florida

THE ROLE OF MODERN EDUCATIONAL TECHNOLOGIES IN TEACHING MEDICAL STUDENTS

The article discusses of the role of modern educational technologies in pedagogical activity of the medical university department, perspectives of formation students' clinical thinking on the basis of competence-oriented approach. The authors analyze innovations accompanying the modern concept of modular education, and propose variants of modern technology usage as a part of education of future doctors. The authors present possibilities of context and problem education when teaching senior students. The article contains the information on the most advanced educational technologies imitating the future occupational activity of medical students and implemented at the department of internal diseases No. 1 of Kursk State Medical University. It is concluded that innovative educational technologies should be certainly used as they enable to organize an educational process so that it is possible to achieve the main purpose - formation of students' professional competences