309 research outputs found
Effect of an Er,Cr:YSGG Laser on the Debonding of Lithium Disilicate Veneers With Four Different Thicknesses
Introduction: The objective of this study was to compare in vitro the resistance and type of failure in the debonding of lithium disilicate veneers with four different thicknesses using an erbium chromium yttrium-scandium gallium-garnet (Er, Cr: YSGG) laser.Methods: Sixty-eight bovine teeth were used to bond round lithium disilicate veneers with a 6-millimeter diameter and four different thicknesses: group 1 (0.4 mm), group 2 (0.8 mm), group 3 (1.2 mm), and group 4 (1.6 mm). Each sample was irradiated with an Er, Cr: YSGG laser with 4 W of power and a frequency of 50 Hz, during 60 seconds, scanning concentrically. The energy density per pulse or fluency applied was 5.33 J/cm2 for the four groups. The samples were subjected to a force in a universal testing machine and then observed under a microscope to determine the type of failure. Data were statistically analyzed with the non-parametric Kruskal-Wallis test.Results: The tendency in the results revealed that the thicker veneers showed more resistance to the debonding process. The debonding strength for group 3 was the highest (5.62 MPa), followed by group 4 (5.20 MPa), then group 2 (0.85 MPa), and finally group 1 (0.0 MPa). The most frequent type of failure was a cohesive failure in cement (CC) for all groups, with 73.53% (P ≤ 0.083).Conclusion: Er, Cr: YSGG laser irradiation influences the debonding of lithium disilicate veneers with different thicknesses: the smaller thickness showed the greater debonding. The thickness of veneers was not associated with the type of failure
Pine Pitch Canker and Insects: Relationships and Implications for Disease Spread in Europe
Producción CientíficaThe fungal pathogen Fusarium circinatum (Nirenberg and O’ Donnell) is the causal agent
of pine pitch canker (PPC) disease, which seriously affects conifer species in forests and nurseries
worldwide. In Europe, PPC is only established in the Iberian Peninsula; however, it is presumed that its
range could expand through the continent in the near future. Infection caused by this fungus requires
open wounds on the tree, including physical damage caused by insects. Therefore, a relationship
probably occurs between PPC and a wide variety of insects. The aim of this review is to outline the
taxonomic and ecological diversity of insect species with high potential association with F. circinatum
in Europe and elsewhere. The insects were classified as vectors, carriers and wounding agents
according to the association level with the PPC disease. In addition, we discuss the insect-mediated
spreading of PPC disease in relation to the different phases of forest stand development, from seeds
and seedlings in nurseries to mature stands. Lastly, to improve our predictive capacities and to
design appropriate intervention measures and strategies for controlling disease dissemination by
insects, variables such as geographic location, time of the year and host species should be considered,European Cooperation in Science and Technology (COST Action FP1406 PINESTRENGTH)Ministerio de Economía, Industria y Competitividad - Fondo Europeo de Desarrollo Regional (project AGL2015-69370-R)Centro de Estudios do Ambiente e do Mar (CESAM) (project UID/AMB/50017/2019)Portuguese Foundation for Science and Technology (projects PTDC/AGR-FOR/2768/2014 , POCI-01-0145-FEDER-016785 , SFRH/BPD/122928/2016)Russian Foundation for Basic Research (grant 17-04-01486)Saint Petersburg State Polytechnical University (project 2019-0420
Histological assessment of nanostructured fibrin-agarose skin substitutes grafted in burnt patients. A time-course study
A previously developed fibrin-agarose skin model—UGRSKIN—showed promising clinical results in severely burnt patients. To determine the histological parameters associated to the biocompatibility and therapeutic effects of this model, we carried out a comprehensive structural and ultrastructural study of UGRSKIN grafted in severely burnt patients after 3 months of follow-up. The grafted epidermis was analogue to native human skin from day 30th onward, revealing well-structured strata with well-differentiated keratinocytes expressing CK5, CK8, CK10, claudin, plakoglobin, filaggrin, and involucrin in a similar way to controls, suggesting that the epidermis was able to mature and differentiate very early. Melanocytes and Langerhans cells were found from day 30th onward, together with a basement membrane, abundant hemidesmosomes and lack of rete ridges. At the dermal layer, we found an interface between the grafted skin and the host tissue at day 30th, which tended to disappear with time. The grafted superficial dermis showed a progressive increase in properly-oriented collagen fibers, elastic fibers and proteoglycans, including decorin, similarly to control dermis at day 60-90th of in vivo follow-up. Blood vessels determined by CD31 and SMA expression were more abundant in grafted skin than controls, whereas lymphatic vessels were more abundant at day 90th. These results contribute to shed light on the histological parameters associated to biocompatibility and therapeutic effect of the UGRSKIN model grafted in patients and demonstrate that the bioengineered skin grafted in patients is able to mature and differentiate very early at the epithelial level and after 60–90 days at the dermal level.Consejería de Salud y Familias, Junta de
AndalucíaGrant/Award Numbers: PE-
0395-2019, PI-0458-2016Consejería de
Transformación Económica, Industria,Conocimiento y Universidades, Grant/Award
Number: B-CTS-450-UGR20Instituto de
Salud Carlos III, Grant/Award Number:
AC17/0001
Expanded Differentiation Capability of Human Wharton's Jelly Stem Cells Toward Pluripotency: A Systematic Review
This study was supported by the Spanish Plan Nacional de
Investigación Científica, Desarrollo e Innovación Tecnológica
(I+D+I) of the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), grants FIS PI17/0391,
PI18/331 and PI18/332 (co-financed by ERDF-FEDER, European Union).Human Wharton’s jelly stem cells (HWJSC) can be efficiently isolated from the umbilical cord, and numerous reports have demonstrated that these cells can differentiate into several cell lineages. This fact, coupled with the high proliferation potential of HWJSC, make them a promising source of stem cells for use in tissue engineering and regenerative medicine. However, their real potentiality has not been established to date. In the present study we carried out a systematic review to determine the multilineage differentiation potential of HWJSC. After a systematic literature search we selected 32 publications focused on the differentiation potential of these cells. Analysis of these studies showed that HWJSC display expanded differentiation potential towards some cell types corresponding to all three embryonic cell layers (ectodermal, mesodermal and endodermal), which is consistent with their constitutive expression of key pluripotency markers such as OCT4, SOX2 and NANOG, and the embryonic marker SSEA4. We conclude that HWJSC can be considered cells in an intermediate state between multipotentiality and pluripotentiality, since their proliferation capability is not unlimited and differentiation to all cell types has not been demonstrated thus far. These findings support the clinical use of HWJSC for the treatment of diseases affecting not only mesoderm-type tissues, but also other cell lineages.Spanish Ministry of Economy and Competitiveness FIS PI17/0391, PI18/331, PI18/332ERDF-FEDER, European Unio
Magnetization reversal signatures in the magnetoresistance of magnetic multilayers
The simultaneous determination of magnetoresistance and vectorial-resolved magnetization hysteresis curves in a spin valve structure reveals distinct magnetoresistive features for different magnetic field orientations, which are directly related to the magnetization reversal processes. Measurements performed in the whole angular range demonstrate that the magnetoresistive response originates from the intrinsic anisotropic angular dependence of the magnetization orientation between the two ferromagnetic layers. This also provides direct proof that the spin-dependent scattering in the bulk of the magnetic layers is at the origin of the magnetoresistive signal
Fibrin and Marine-Derived Agaroses for the Generation of Human Bioartificial Tissues: An Ex Vivo and In Vivo Study
Development of an ideal biomaterial for clinical use is one of the main objectives of current
research in tissue engineering. Marine-origin polysaccharides, in particular agaroses, have been
widely explored as scaffolds for tissue engineering. We previously developed a biomaterial based on
a combination of agarose with fibrin, that was successfully translated to clinical practice. However,
in search of novel biomaterials with improved physical and biological properties, we have now
generated new fibrin-agarose (FA) biomaterials using 5 different types of agaroses at 4 different
concentrations. First, we evaluated the cytotoxic effects and the biomechanical properties of these
biomaterials. Then, each bioartificial tissue was grafted in vivo and histological, histochemical and
immunohistochemical analyses were performed after 30 days. Ex vivo evaluation showed high
biocompatibility and differences in their biomechanical properties. In vivo, FA tissues were biocompatible
at the systemic and local levels, and histological analyses showed that biointegration
was associated to a pro-regenerative process with M2-type CD206-positive macrophages. These
results confirm the biocompatibility of FA biomaterials and support their clinical use for the generation
of human tissues by tissue engineering, with the possibility of selecting specific agarose
types and concentrations for applications requiring precise biomechanical properties and in vivo
reabsorption times.Hispanagar SA, Burgos, Spain, through CDTI, Ministry of Science and Innovation, Spain, Programa Operativo Plurirregional de Crecimiento Inteligente (CRIN) IDI-20180052Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I + D + I) of the Spanish Ministry of Science and Innovation (Instituto de Salud Carlos III) FIS PI20/0317
FIS PI20/0318
FIS PI21/0980
ICI19/00024
ICI21/00010Junta de Andalucia PE-0395-2019
PI-0442-2019
PI-0086-2020Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades B-CTS-504-UGR2
Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis
The structure of the human skin is directly dependent on its location and the mechanical forces to which it is subjected. In
the present work, we have performed a comprehensive analysis of the human ridged and non-ridged skin to identify the
differences and similarities between both skin types. For this purpose, human skin samples were obtained from dorsal hand
skin (DHS), palmar hand skin (PHS), dorsal foot skin (DFS) and plantar foot skin (PFS) from the same cadaveric donors.
Histological, histochemical and semiquantitative and quantitative immunohistochemical analyses were carried out to evaluate
the epidermis, dermis and basement membrane. Results show that the epithelial layer of ridged skin had larger cell number
and size than non-ridged skin for most strata. Melanocytes and Langerhans cells were more abundant in non-ridged skin,
whereas Merkel cells were preferentially found in ridged skin. The expression pattern of CK5/6 was slightly differed between
non-ridged and ridged skin. Involucrin expression was slightly more intense in non-ridged skin than in ridged skin. Collagen
was more abundant in foot skin dermis than in hand skin, and in ridged skin as compared to non-ridged skin. Elastic fibers
were more abundant in DHS. Biglycan was more abundant in foot skin than in hand skin. No differences were found for
blood and lymphatic vessels. The basement membrane laminin was preferentially found in foot skin. These results revealed
important differences at the epithelial, dermal and basement membrane levels that could contribute to a better knowledge
of the human skin histology.This work was partially supported by Award no.
AC17/00013 (NanoGSkin) by ISCIII thorough AES 2017 and within
the EuroNanoMed framework
The Fourteenth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the extended Baryon Oscillation Spectroscopic Survey and from the second phase of the Apache Point Observatory Galactic Evolution Experiment
The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in
operation since July 2014. This paper describes the second data release from
this phase, and the fourteenth from SDSS overall (making this, Data Release
Fourteen or DR14). This release makes public data taken by SDSS-IV in its first
two years of operation (July 2014-2016). Like all previous SDSS releases, DR14
is cumulative, including the most recent reductions and calibrations of all
data taken by SDSS since the first phase began operations in 2000. New in DR14
is the first public release of data from the extended Baryon Oscillation
Spectroscopic Survey (eBOSS); the first data from the second phase of the
Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2),
including stellar parameter estimates from an innovative data driven machine
learning algorithm known as "The Cannon"; and almost twice as many data cubes
from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous
release (N = 2812 in total). This paper describes the location and format of
the publicly available data from SDSS-IV surveys. We provide references to the
important technical papers describing how these data have been taken (both
targeting and observation details) and processed for scientific use. The SDSS
website (www.sdss.org) has been updated for this release, and provides links to
data downloads, as well as tutorials and examples of data use. SDSS-IV is
planning to continue to collect astronomical data until 2020, and will be
followed by SDSS-V.Comment: SDSS-IV collaboration alphabetical author data release paper. DR14
happened on 31st July 2017. 19 pages, 5 figures. Accepted by ApJS on 28th Nov
2017 (this is the "post-print" and "post-proofs" version; minor corrections
only from v1, and most of errors found in proofs corrected
La experiencia de formación en intervención terapéutica: reflexiones suscitadas durante el entrenamiento de la Especialización en Terapia Familiar de la Universidad Católica Luis Amigó
This paper establishes some reflections as result of a training exercise in the family therapeutic intervention carried out with families, during the first semester of the Family Therapy specialization program at Universidad Católica Luis Amigó. This exercise brings about meaning issues such as: the understanding of one´s self, communication as a possibility, the use of techniques and observation experiences in early sessions with families. The analysis of this training experience, is based on conceptual and life elements that are fundamental in family therapy that try influence the professional exercise under a systematic epistemology with a theoretical basis and a true reflective process.El presente artículo plantea reflexiones resultado de la experiencia de entrenamiento en intervención terapéutica con familias, llevada a cabo durante el primer semestre de formación como especialistas en terapia familiar de la Universidad Católica Luis Amigó. En el ejercicio se identifican asuntos significativos como la comprensión del sí mismo (self), la comunicación como posibilidad, el uso de técnicas y la experiencia de observación en las primeras sesiones con familias. Al plantear reflexiones suscitadas durante la práctica de entrenamiento, se retoman elementos conceptuales y vivenciales que articulados se consideran eje central del terapeuta familiar que busca conducir el ejercicio profesional bajo una epistemología sistémica con asiento conceptual y un verdadero proceso reflexivo
La comunicación como espacio de resistencia
Actores sociales, medios comunitarios e interculturalidad son fundamentales para entender a la comunicación como espacio de resistencia. Esta publicación reúne una serie de de investigaciones en distintos contextos en los cuales la comunicación cumple un papel fundamental en términos del derechos a la resistencia. Plantea una discusión teórica y aborda la comunicación desde el ejercicio de participación democrática
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