Influence of Carbon Source on the Production of Extracellular Ligninolytic Enzymes by Phanerochaete chrysosporium

The effect of altering the carbon source in the growing environment was investigated relative to the production of ligninolytic enzymes by Phanerochaete chrysosporium. Glucose, cellobiose, and cellulose (or mixtures thereof) were used as the carbon sources. Glucose oxidase and glyoxal oxidase activities in all carbon sources were produced during cultivation. High peak levels (0.17 to 0.24 IU/mL) of manganese peroxidase activity were observed only in mediums containing oligosaccharides. Lignin peroxidase activity was high in glucose medium (0.21 IU/mL of peak value); however, minimal amounts were formed in the cellulose medium (0.01 IU/mL of peak value). High amounts of cellobiose:quinone oxidoreductase (3.33-3.99 IU/mL of peak value) and cellobiose dehydrogenase (0.04-0.2 IU/mL of peak value) were measured when P. chrysosporium was grown on a medium containing cellulose. This work discovered that the mixture of glucose and cellulose as a carbon source favored high co-production of ligninolytic enzymes by P. chrysosporium

Biomechanical Pulping of Corn Stalk Rind with a White Rot Fungus – Trametes hirsuta – and the Use of Delignified Corn Stalk Pith as a Pulp Additive

Corn stalk rind (CSR) was treated with Trametes hirsuta lg-9 and then refined into pulp. The biotreatment resulted in loss of paper strength and brightness, but energy consumption during refining (ECR) was reduced. Meanwhile, multiple linear regression was carried out, for which ECR served as the dependent variable, and the yield and infrared relative absorbance intensities at 3414 cm-1 and 1653 cm-1 of the biotreated CSR served as independent variables. Results showed that the determining parameters of the biotreated CSR may be used to predict the ECR. In this work, delignified corn stalk pith (CSP) was added to aspen alkaline hydrogen peroxide mechanical pulp (APMP). The CSP enhanced the strength properties of the aspen APMP and inhibited yellowing. The biomechanical pulping of CSR has the potential to produce a low-cost green pulp, and the delignified CSP can serve as a pulp additive

Research methods for reduced-impact logging: workshop results

In July 1996, with assistance from FAO, USAID, and the USDA Forest Service, CIFOR offered its first International Research Training Seminar on Reduced-Impact Timber Harvesting and Natural Forest Management. Participants were drawn from ten countries and represented disciplines ranging from silviculture and hydrology to wildlife biology. Throughout the workshop, field exercises were integrated with classroom activities, initially in the experimental forest plantation near CIFOR's headquarters, and later at an industrial timber concession in East Kalimantan. Participants also carried out a one-day research project on some aspect of forest management. Their written reports constitute the bulk of this publication and have been grouped by subject matter. The first group comprises seven papers concerned with soil compaction and erosion; the second consists of ten papers, one on tree damage and the remainder on the density or growth of trees occurring in different situations and under different conditions

Additional file 4: of Transcriptome analysis of different growth stages of Aspergillus oryzae reveals dynamic changes of distinct classes of genes during growth

Table S3. The expression levels and functional annotation of DEGs between Ao_24 and Ao_72. (XLS 1836 kb

Additional file 5: of Transcriptome analysis of different growth stages of Aspergillus oryzae reveals dynamic changes of distinct classes of genes during growth

Table S4. The expression levels and functional annotation of DEGs between Ao_48 and Ao_72. (XLS 215 kb

Additional file 6: of Transcriptome analysis of different growth stages of Aspergillus oryzae reveals dynamic changes of distinct classes of genes during growth

Table S1. qRT-PCR primers used in this study. (DOCX 16 kb