Coherent beam superposition of ten diode lasers with a Dammann grating

We demonstrate the use of a binary diffractive optical element in a very simple setup to convert the multilobed beam from a low fill factor array of coherent laser diodes into a quasi-Gaussian beam. The phase profile of the grating is determined with a phase retrieval algorithm. Experimentally, the conversion efficiency reaches more than 44%. We also establish that this setup can be used to make an effective measurement of the coherency of the laser array

Modélisation de l'impact de projectiles sur des installations voisines : couplages mécano-probabiliste

National audienceLes projectiles engendrés lors d'une explosion d'un réservoir sont des menaces pour d'autres équipements environnants et peuvent entraîner des explosions successives

On the numerical modelling of the Jet Erosion Test

International audienceEvaluating the erodibility of a soil, both in terms of erosion threshold (initiation) and erosion rate (progression), is critical for the evaluation of the safety of water retaining structures. Indeed different soils can erode at different rates. However, the relationship between the erosion parameters and the geotechnical and chemical properties of soils remains largely unknown. The jet erosion test appears to be an efficient and simple means for quantifying the two erosion parameters involved. The first parameter is the critical stress while the second parameter is the erosion coefficient. A simplified model of this test has been drawn up by G. Hanson et al. to interpret the experimental curves. Few attempts have been made so far to model the whole process, however. The aim of this study is to simulate the impinging jet and to take into account the erosion of the soil by means of computational fluid dynamics (CFD) numerical modelling. The key point was the time dependence of the problem, due to erosion processes, however the turbulent flow could be considered as steady because of the assumption of low kinetics erosion assumption. The results of the present modelling study are compared to the simplified model and to experimental data. This comparison is a first confirmation of the validity of the simplified model as a means of assessing the critical stress and the erosion coefficient with jet erosion tests

The domino effect and integrated probabilistic approaches for risk analysis

International audienceThe paper develops a probabilistic approach in order to deal with domino effects that may occur in industrial facilities : an explosion or accident may generate various sets of projectiles that may impact other existing facilities (tanks under high-pressure, etc) and may generate other sets of projectiles and so on. Three main parts are considered : 1- Source term : for the first set of generated projectiles, probabilistic distributions are considered for the number, masses, velocities, departure angles, geometrical form, dimensions, and constitutive materials properties. The authors have collected existing models from the literature. 2- Target term : for the set of impacted targets, probabilistic distributions are considered for the number of impacting projectiles, velocities, incidence angles and energy at impact, constitutive materials properties, dimensions of the impacted targets, and projectiles penetration depths into the targets. In this paper, new models for the impact are proposed to calculate the penetration depth after impact : case of cylindrical rods impacting rectangular plates, both are metal made. The theoretical results are compared to the experimental data (4 data sets) collected from the literature with the following features : projectiles mass ranging from 0.1g up to 250 kg, projectiles velocity ranging from 10 m/s up to 2100 m/s, projectiles diameters ranging from 1.5 mm up to 90 mm, target strength ranging from 300 MPa up to 1400 MPa and incidence angles ranging from 0 degree up to 70 degrees. 3- Domino effect term : evaluation of the risks of second set of explosions that may take place in the impacted components. Monte Carlo simulations are used in order to calculate the different probabilities : probability of impact, distribution of the penetration depth and probability of domino effect

Le matériau sol-chaux comme une matériau résistant à l'érosion pour les ouvrages hydrauliques : état de l'art et projet de R&D français DigueElite

International audienceThe treatment of silty and clayey soils with lime (calcium oxide or hydroxide) is a technique widely used for soils improvement and stabilization for construction of roads, highways, railways, platforms. However, the principles of lime treatment for hydraulic earthen structures remains barely applied or even forgotten (European case). If existing testimonials (levees, dams, mainly in US and Australia) are evidences that show effectiveness and durability of lime-treated structures, there was a necessity to evaluate a series of unknown characteristics and relevant properties of lime-treated soils for an application in hydraulic context, through laboratory studies and full-scale experiments

Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain

The ability of Lactococcus lactis to adhere to the intestinal mucosa can potentially prolong the contact with the host, and therefore favour its persistence in the gut. In the present study, the contribution of plasmid-encoded factors to the adhesive and transit properties of the L. lactis subsp. cremoris IBB477 strain was investigated. Plasmid-cured derivatives as well as deletion mutants were obtained and analysed. Adhesion tests were performed using non-coated polystyrene plates, plates coated with mucin or fibronectin and mucus-secreting HT29-MTX intestinal epithelial cells. The results indicate that two plasmids, pIBB477a and b, are involved in adhesion of the IBB477 strain. One of the genes localised on plasmid pIBB477b (AJ89_14230), which encodes cell wall-associated peptidase S8 (PrtP), mediates adhesion of the IBB477 strain to bare, mucin- and fibronectincoated polystyrene, as well as to HT29-MTX cells. Interactions between bacteria and mucus secreted by HT29- MTX cells were further investigated by fluorescent staining and confocal microscopy. Confocal images showed that IBB477 forms dense clusters embedded in secreted mucus. Finally, the ability of IBB477 strain and its ΔprtP deletion mutant to colonise the gastrointestinal tract of conventional C57Bl/6mice was determined. Both strains were present in the gut for up to 72 h. In summary, adhesion and persistence of IBB477 were analysed by in vitro and in vivo approaches, respectively. Our studies revealed that plasmidic genes encoding cell surface proteins are more involved in the adhesion of IBB477 strain than in the ability to confer a selective advantage in the gut

Development of Temperature-Controlled Shear Tests to Reproduce White-Etching-Layer Formation in Pearlitic Rail Steel

The formation of a white etching layer (WEL), a very hard and brittle phase on the rail surface, is associated with a progressive transformation of the pearlitic grain to very fragmented grains due to the cumulative passage of trains. Its formation is associated with a complex thermomechanical coupling. To predict the exact conditions of WEL formation, a thermomechanical model previously proposed by the authors needs to be validated. In this study, monotonic and cyclic shear tests using hat-shaped specimens were conducted in the temperature range of 20 C to 400 C to reproduce the WEL formation. The tests showed a strong sensitivity of the material to temperature, which does not necessarily favor WEL formation. For the monotonic tests, no WELs were produced; however, a localization of the plastic deformation was observed for tests performed at 200 C and 300 C. In this temperature range, the material was less ductile than at room temperature, leading to failure before WEL formation. At 400 C, the material exhibited a much more ductile behavior, and nanograins close to WEL stages were visible. For the cyclic tests, a WEL zone was successfully reproduced at room temperature only and confirmed the effect of shear in WEL formation. The same cyclic tests conducted at 200 C and 300 C yielded results consistent with those of the monotonic tests; the deformation was much more localized and did not lead to WEL formation.This work is part of the multi-disciplinary project MOPHAB, which aims to improve our knowledge and understanding of the mechanisms leading to the formation of the white etching layer in the materials used to construct railways and to develop corresponding numerical models. This project was supported by IRT Railenium and other industrial partners (RATP: Régie Autonome des Transports Parisiens, France, SNCF: Société Nationale des Chemins de Fer Francais, France, SAARSTAHL rail)

Caveolin-1 protects B6129 mice against Helicobacter pylori gastritis.

Caveolin-1 (Cav1) is a scaffold protein and pathogen receptor in the mucosa of the gastrointestinal tract. Chronic infection of gastric epithelial cells by Helicobacter pylori (H. pylori) is a major risk factor for human gastric cancer (GC) where Cav1 is frequently down-regulated. However, the function of Cav1 in H. pylori infection and pathogenesis of GC remained unknown. We show here that Cav1-deficient mice, infected for 11 months with the CagA-delivery deficient H. pylori strain SS1, developed more severe gastritis and tissue damage, including loss of parietal cells and foveolar hyperplasia, and displayed lower colonisation of the gastric mucosa than wild-type B6129 littermates. Cav1-null mice showed enhanced infiltration of macrophages and B-cells and secretion of chemokines (RANTES) but had reduced levels of CD25+ regulatory T-cells. Cav1-deficient human GC cells (AGS), infected with the CagA-delivery proficient H. pylori strain G27, were more sensitive to CagA-related cytoskeletal stress morphologies ("humming bird") compared to AGS cells stably transfected with Cav1 (AGS/Cav1). Infection of AGS/Cav1 cells triggered the recruitment of p120 RhoGTPase-activating protein/deleted in liver cancer-1 (p120RhoGAP/DLC1) to Cav1 and counteracted CagA-induced cytoskeletal rearrangements. In human GC cell lines (MKN45, N87) and mouse stomach tissue, H. pylori down-regulated endogenous expression of Cav1 independently of CagA. Mechanistically, H. pylori activated sterol-responsive element-binding protein-1 (SREBP1) to repress transcription of the human Cav1 gene from sterol-responsive elements (SREs) in the proximal Cav1 promoter. These data suggested a protective role of Cav1 against H. pylori-induced inflammation and tissue damage. We propose that H. pylori exploits down-regulation of Cav1 to subvert the host's immune response and to promote signalling of its virulence factors in host cells

Search for the standard model Higgs boson in the H to ZZ to 2l 2nu channel in pp collisions at sqrt(s) = 7 TeV

A search for the standard model Higgs boson in the H to ZZ to 2l 2nu decay channel, where l = e or mu, in pp collisions at a center-of-mass energy of 7 TeV is presented. The data were collected at the LHC, with the CMS detector, and correspond to an integrated luminosity of 4.6 inverse femtobarns. No significant excess is observed above the background expectation, and upper limits are set on the Higgs boson production cross section. The presence of the standard model Higgs boson with a mass in the 270-440 GeV range is excluded at 95% confidence level.Comment: Submitted to JHE

Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV

A search for a Higgs boson decaying into two photons is described. The analysis is performed using a dataset recorded by the CMS experiment at the LHC from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross section of the standard model Higgs boson decaying to two photons. The expected exclusion limit at 95% confidence level is between 1.4 and 2.4 times the standard model cross section in the mass range between 110 and 150 GeV. The analysis of the data excludes, at 95% confidence level, the standard model Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The largest excess of events above the expected standard model background is observed for a Higgs boson mass hypothesis of 124 GeV with a local significance of 3.1 sigma. The global significance of observing an excess with a local significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is estimated to be 1.8 sigma. More data are required to ascertain the origin of this excess.Comment: Submitted to Physics Letters