Long-term impact of maternal high-fat diet on offspring cardiac health: role of micro-RNA biogenesis.

Heart failure is a worldwide leading cause of death. Diet and obesity are particularly of high concern in heart disease etiology. Gravely, altered nutrition during developmental windows of vulnerability can have long-term impact on heart health; however, the underlying mechanisms are poorly understood. In the understanding of the initiation of chronic diseases related to developmental exposure to environmental challenges, deregulations in epigenetic mechanisms including micro-RNAs have been proposed as key events. In this context, we aimed at delineating the role of micro-RNAs in the programming of cardiac alterations induced by early developmental exposure to nutritional imbalance. To reach our aim, we developed a human relevant model of developmental exposure to nutritional imbalance by maternally exposing rat to high-fat diet during gestation and lactation. In this model, offspring exposed to maternal high-fat diet developed cardiac hypertrophy and increased extracellular matrix depot compared to those exposed to chow diet. Microarray approach performed on cardiac tissue allowed the identification of a micro-RNA subset which was down-regulated in high-fat diet-exposed animals and which were predicted to regulate transforming growth factor-beta (TGFβ)-mediated remodeling. As indicated by in vitro approaches and gene expression measurement in the heart of our animals, decrease in DiGeorge critical region 8 (DGCR8) expression, involved in micro-RNA biogenesis, seems to be a critical point in the alterations of the micro-RNA profile and the TGFβ-mediated remodeling induced by maternal exposure to high-fat diet. Finally, increasing DGCR8 activity and/or expression through hemin treatment in vitro revealed its potential in the rescue of the pro-fibrotic phenotype in cardiomyocytes driven by DGCR8 decrease. These findings suggest that cardiac alterations induced by maternal exposure to high-fat diet is related to abnormalities in TGFβ pathway and associated with down-regulated micro-RNA processing. Our study highlighted DGCR8 as a potential therapeutic target for heart diseases related to early exposure to dietary challenge

The universal distribution of halo interlopers in projected phase space. Bias in galaxy cluster concentration and velocity anisotropy?

When clusters of galaxies are viewed in projection, one cannot avoid picking up foreground/background interlopers (FBIs), that lie within the virial cone (VC), but outside the virial sphere. Structural & kinematic deprojection equations are not known for an expanding Universe, where the Hubble flow (HF) stretches the line-of-sight (LOS) distribution of velocities. We analyze 93 mock relaxed clusters, built from a cosmological simulation. The stacked mock cluster is well fit by an m=5 Einasto DM density profile (but only out to 1.5 virial radii [r_v]), with velocity anisotropy (VA) close to the Mamon-Lokas model with VA radius equal to that of density slope -2. The surface density of FBIs is nearly flat out to r_v, while their LOS velocity distribution shows a dominant gaussian cluster-outskirts component and a flat field component. This distribution of FBIs in projected phase space is nearly universal in mass. A local k=2.7 sigma velocity cut returns the LOS velocity dispersion profile (LOSVDP) expected from the NFW density and VA profiles measured in 3D. The HF causes a shallower outer LOSVDP that cannot be well matched by the Einasto model for any k. After this velocity cut, FBIs still account for 23% of DM particles within the VC (close to the observed fraction of cluster galaxies lying off the Red Sequence). The best-fit projected NFW/Einasto models underestimate the 3D concentration by 6+/-6% (16+/-7%) after (before) the velocity cut, unless a constant background is included in the fit. Assuming the correct mass profile, the VA profile is well recovered from the measured LOSVDP, with a slight bias towards more radial orbits in the outer regions. These small biases are overshadowed by large cluster-cluster variations caused by cosmic variance. An appendix provides an analytical approximation to the surface density, projected mass and tangential shear profiles of the Einasto model.Comment: Version published in A&A, 23 pages, 22 figure

The Spitzer Extragalactic Representative Volume Survey (SERVS): The Environments of High-z SDSS Quasi-Stellar-Objects

This paper presents a study of the environments of SDSS Quasi-Stellar-Objects (QSOs) in the Spitzer Extragalactic Representative Volume Survey (SERVS). We concentrate on the high-redshift QSOs as these have not been studied in large numbers with data of this depth before. We use the IRAC 3.6-4.5{\mu}m colour of objects and ancillary r-band data to filter out as much foreground contamination as possible. This technique allows us to find a significant (> 4-{\sigma}) over-density of galaxies around QSOs in a redshift bin centred on z ~ 2.0 and a (> 2-{\sigma}) over-density of galaxies around QSOs in a redshift bin centred on z ~ 3.3. We compare our findings to the predictions of a semi-analytic galaxy formation model, based on the {\Lambda}CDM millennium simulation, and find for both redshift bins that the model predictions match well the source-density we have measured from the SERVS data.Comment: 13 pages, 12 figures, Accepted by Ap

Suppressed radio emission in supercluster galaxies: enhanced ram pressure in merging clusters?

The environmental influence on the 1.4 GHz continuum radio emission of galaxies is analyzed in a 600 deg2 region of the local Universe containing the Shapley Supercluster (SSC). Galaxies in the FLASH and 6dFGS redshift surveys are cross-identified with NVSS radio sources, selected in a subsample doubly complete in volume and luminosity. Environmental effects are studied through a smoothed density field (normalized with random catalogs with the same survey edges and redshift selection function) and the distance to the nearest cluster (R/r200, where r200 is the virial radius, whose relation to the aperture velocity dispersion is quantified). The fraction of high radio loudness (R_K=L_radio/L_K) galaxies in the 10 Mpc Abell 3558 cluster complex at the core of the SSC (SSC-CR) is half as large than elsewhere. In the SSC-CR, R_K is anti-correlated with the density of the large-scale environment and correlated with R/r200: central brightest cluster galaxies (BCGs) in the SSC-CR are 10x less radio-loud than BCGs elsewhere, with signs of suppressed radio loudness in the SSC-CR also present beyond the BCGs, out to at least 0.3 r200. This correlation is nearly as strong as the tight correlation of L_K with R/r200 (K-luminosity segregation), inside the SSC-CR. The suppression of radio loudness in SSC-CR BCGs can be attributed to cluster-cluster mergers that destroy the cool core and thus the supply of gas to the central AGN. We analytically demonstrate that the low radio loudness of non-BCG galaxies within SSC-CR clusters cannot be explained by direct major galaxy mergers or rapid galaxy flyby collisions, but by the loss of gas supply through the enhanced ram pressure felt when these galaxies cross the shock front between the 2 merging clusters and are later subjected to the stronger wind from the 2nd cluster.Comment: Version consolidated with Erratum A&A 499, 4

Protective CD8+ T lymphocytes in Primates Immunized with Malaria Sporozoites

Live attenuated malaria vaccines are more potent than the recombinant protein, bacterial or viral platform vaccines that have been tested, and an attenuated sporozoite vaccine against falciparum malaria is being developed for humans. In mice, attenuated malaria sporozoite vaccines induce CD8+ T cells that kill parasites developing in the liver. We were curious to know if CD8+ T cells were also important in protecting primates against malaria. We immunized 9 rhesus monkeys with radiation attenuated Plasmodium knowlesi sporozoites, and found that 5 did not develop blood stage infections after challenge with live sporozoites. We then injected 4 of these protected monkeys with cM-T807, a monoclonal antibody to the CD8 molecule which depletes T cells. The fifth monkey received equivalent doses of normal IgG. In 3 of the 4 monkeys receiving cM-T807 circulating CD8+ T cells were profoundly depleted. When re-challenged with live sporozoites all 3 of these depleted animals developed blood stage malaria. The fourth monkey receiving cM-T807 retained many circulating CD8+ T cells. This monkey, and the vaccinated monkey receiving normal IgG, did not develop blood stage malaria at re-challenge with live sporozoites. Animals were treated with antimalarial drugs and rested for 4 months. During this interval CD8+ T cells re-appeared in the circulation of the depleted monkeys. When all vaccinated animals received a third challenge with live sporozoites, all 5 monkeys were once again protected and did not develop blood stage malaria infections. These data indicate that CD8+ T cells are important effector cells protecting monkeys against malaria sporozoite infection. We believe that malaria vaccines which induce effector CD8+ T cells in humans will have the best chance of protecting against malaria

On lattice profile of the elliptic curve linear congruential generators

Lattice tests are quality measures for assessing the intrinsic structure of pseudorandom number generators. Recently a new lattice test has been introduced by Niederreiter and Winterhof. In this paper, we present a general inequality that is satisfied by any periodic sequence. Then, we analyze the behavior of the linear congruential generators on elliptic curves (EC-LCG) under this new lattice test and prove that the EC-LCG passes it up to very high dimensions. We also use a result of Brandstätter and Winterhof on the linear complexity profile related to the correlation measure of order k to present lower bounds on the linear complexity profile of some binary sequences derived from the EC-LCG

The Properties of the Heterogeneous Shakhbazyan Groups of Galaxies in the SDSS

We present a systematic study of the sub-sample of Shakhbazyan groups (SHKs) covered by the Sloan Digital Sky Survey Data Release--5 (SDSS-5). SHKs probe an environment with characteristics which are intermediate between those of loose and very compact groups. Surprisingly, we found that several groups identifying algorithms (e.g. Berlind et al. 2006, Tago et al. 2008) miss this type of structures. Using the SDSS-5 spectroscopic data and the photometric redshifts derived in D'Abrusco et al. 2007, we identified possible group members in photometric redshift space and derived, for each group, several individual properties. We also combined pointed and stacked Rosat All Sky Survey data to investigate the X-ray luminosities of these systems. Our study confirms that the majority of groups are physical entities with richness in the range 3--13 galaxies, and properties ranging between those of loose and compact groups. We confirm that SHK groups are richer in early-type galaxies than the surrounding environment and the field, as expected from the morphology-density relation and from the selection of groups of red galaxies. Furthermore, our work supports the existence of two sub-classes of structures, the first one being formed by compact and isolated groups and the second formed by extended structures. We suggest that while the first class of objects dwells in less dense regions like the outer parts of clusters or the field, possibly sharing the properties of Hickson Compact Groups, the more extended structures represent a mixture of [core+halo] configurations and cores of rich clusters. X-ray luminosities for SHKs are generally consistent with these results and with the expectations for the L_X-sigma_v relation, but also suggest the velocity dispersions reported in literature are underestimated for some of the richest systems.Comment: 20 pages, 14 figures, 4 tables. Accepted for publication by MNRA

NEOWISE Observations of Near-Earth Objects: Preliminary Results

With the NEOWISE portion of the \emph{Wide-field Infrared Survey Explorer} (WISE) project, we have carried out a highly uniform survey of the near-Earth object (NEO) population at thermal infrared wavelengths ranging from 3 to 22 $\mu$m, allowing us to refine estimates of their numbers, sizes, and albedos. The NEOWISE survey detected NEOs the same way whether they were previously known or not, subject to the availability of ground-based follow-up observations, resulting in the discovery of more than 130 new NEOs. The survey's uniformity in sensitivity, observing cadence, and image quality have permitted extrapolation of the 428 near-Earth asteroids (NEAs) detected by NEOWISE during the fully cryogenic portion of the WISE mission to the larger population. We find that there are 981$\pm$19 NEAs larger than 1 km and 20,500$\pm$3000 NEAs larger than 100 m. We show that the Spaceguard goal of detecting 90% of all 1 km NEAs has been met, and that the cumulative size distribution is best represented by a broken power law with a slope of 1.32$\pm$0.14 below 1.5 km. This power law slope produces $\sim13,200\pm$1,900 NEAs with $D>$140 m. Although previous studies predict another break in the cumulative size distribution below $D\sim$50-100 m, resulting in an increase in the number of NEOs in this size range and smaller, we did not detect enough objects to comment on this increase. The overall number for the NEA population between 100-1000 m are lower than previous estimates. The numbers of near-Earth comets will be the subject of future work.Comment: Accepted to Ap