Peptide profiling by capillary separation techniques coupled to mass spectrometry

This thesis describes very sensitive methods for peptide detection, obtained by the coupling of high efficiency capillary chromatographic techniques to mass spectrometry. The application of novel data preprocessing methods to the analysis of such complex MS data generated is also described. The data analysis step was completed by the use of multivariate statistical analysis tools, such as principal component analysis (PCA). The developed platform could be applied to the study of complex peptide mixtures and of proteomic samples.TNO Quality of LifeUBL - phd migration 201

Thermal management of a Formula E electric motor: Analysis and optimization

The thermal analysis of a high performance brushless synchronous electric motor with permanent magnets and water jacket cooling is presented. The analysis is carried out following a lumped parameter thermal network approach which allows to identify the most important thermal paths in the motor and the main parameters influencing them. Thanks to its simplicity, the solution of such a thermal network model is very fast, allowing a large number of what-if scenarios to be computed over a short amount of time. For this reason, the model is coupled with external tools for performing systematic sensitivity analyses and optimizations. Goal of the investigation is the reduction of the windings temperature being this temperature inversely proportional to the efficiency and the power delivered by the motor. The sensitivity analysis, performed over a series of material, geometric, and operational factors, leads to the identification of the most relevant parameters influencing the thermal behaviour of the motor. A series of optimizations, focusing on these parameters and including suitable constraints granting the well-posedness of the problem and the feasibility of the solution, bring to the definition of an optimum layout of the water jacket and of the stator geometries. The optimized geometry allows a significant reduction of the windings temperature to be achieved

Migraciones, comunidades etnificadas y consumo de alcohol

Este trabajo analiza, desde una perspectiva socioantropológica, el papel que adquieren algunos patrones de consumo de alcohol asociados a la masculinidad entre la comunidad ecuatoriana en Génova, Italia. La práctica del consumo de alcohol, en contextos homosociales de migración y asociada a actividades deportivas, está (re)significada bajo parámetros culturales instituidos como “ritualidad alcohólica de la masculinidad”. Alejándonos de un enfoque de patologización del consumo, y adentrándonos en el estudio de las “culturas del alcohol”, migración y consumo, exploramos sobre los significados del tomar ecuatorianizado en tres escenarios de identidad cultural en tanto que estrategias de territorialización

Highly efficient human serum filtration with water-soluble nanoporous nanoparticles

Antonella Pujia1, Francesco De Angelis1,2, Domenica Scumaci3, Marco Gaspari3, Carlo Liberale1,2, Patrizio Candeloro1, Giovanni Cuda3, Enzo Di Fabrizio1,21BIONEM Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro “Magna Graecia”, Germaneto (CZ), Italy; 2IIT, Italian Institute of Technology, Genova, Italy; 3Proteomics and Mass Spectrometry Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro “Magna Graecia”, Germaneto (CZ), ItalyBackground: Human serum has the potential to become the most informative source of novel biomarkers, but its study is very difficult due to the incredible complexity of its molecular composition. We describe a novel tool based on biodegradable nanoporous nanoparticles (NPNPs) that allows the harvesting of low-molecular-weight fractions of crude human serum or other biofluids. NPNPs with a diameter of 200 nm and pore size of a few nm were obtained by ultrasonication of nanoporous silicon. When incubated with a solution, the NPNPs harvest only the molecules small enough to be absorbed into the nanopores. Then they can be recovered by centrifugation and dissolved in water, making the harvested molecules available for further analyses.Results: Fluorescence microscopy, gel electrophoresis, and mass spectrometry were used to show the enrichment of low-molecular-weight fraction of serum under physiological conditions, with a cut-off of 13 kDa and an enrichment factor >50.Conclusion: From these findings, we conclude that ability to tune pore size, combined with the availability of hundreds of biomolecule cross-linkers, opens up new perspectives on complex biofluid analysis, discovery of biomarkers, and in situ drug delivery.Keywords: nanoporous silicon, nanoparticle, biomarker discovery, human serum proteomics, harvestin

Chlamydia trachomatis and Neisseria gonorrhoeae rectal infections: Interplay between rectal microbiome, HPV infection and Torquetenovirus

Men having sex with men (MSM) represent a key population, in which sexually transmitted rectal infections (STIs) caused by Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and high-risk HPV (HR-HPV) are very common and linked to significant morbidity. Investigating the anorectal microbiome associated with rectal STIs holds potential for deeper insights into the pathogenesis of these infections and the development of innovative control strategies. In this study, we explored the interplay at the rectal site between C. trachomatis, N. gonorrhoeae, HR-HPV infection, and the anorectal microbiome in a cohort of 92 MSM (47 infected by CT and/or NG vs 45 controls). Moreover, we assessed the presence of Torquetenovirus (TTV), a non-pathogenic endogenous virus, considered as a possible predictor of immune system activation. We found a high prevalence of HR-HPV rectal infections (61%), especially in subjects with a concurrent CT/NG rectal infection (70.2%) and in people living with HIV (84%). In addition, we observed that TTV was more prevalent in subjects with CT/NG rectal infections than in non-infected ones (70.2% vs 46.7%, respectively). The anorectal microbiome of patients infected by CT and/or NG exhibited a reduction in Escherichia, while the presence of TTV was significantly associated with higher levels of Bacteroides. We observed a positive correlation of HR-HPV types with Escherichia and Corynebacterium, and a negative correlation with the Firmicutes phylum, and with Prevotella, Oscillospira, Sutterella. Our findings shed light on some of the dynamics occurring within the rectal environment involving chlamydial/gonococcal infections, HPV, TTV, and the anorectal microbiome. These data could open new perspectives for the control and prevention of STIs in MSM

Natural humoral immune response to ribosomal P0 protein in colorectal cancer patients

Tumor associated antigens are useful in colorectal cancer (CRC) management. The ribosomal P proteins (P0, P1, P2) play an important role in protein synthesis and tumor formation. The immunogenicity of the ribosomal P0 protein in head and neck, in breast and prostate cancer patients and the overexpression of the carboxyl-terminal P0 epitope (C-22 P0) in some tumors were reported

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]

Alignment of the ALICE Inner Tracking System with cosmic-ray tracks

37 pages, 15 figures, revised version, accepted by JINSTALICE (A Large Ion Collider Experiment) is the LHC (Large Hadron Collider) experiment devoted to investigating the strongly interacting matter created in nucleus-nucleus collisions at the LHC energies. The ALICE ITS, Inner Tracking System, consists of six cylindrical layers of silicon detectors with three different technologies; in the outward direction: two layers of pixel detectors, two layers each of drift, and strip detectors. The number of parameters to be determined in the spatial alignment of the 2198 sensor modules of the ITS is about 13,000. The target alignment precision is well below 10 micron in some cases (pixels). The sources of alignment information include survey measurements, and the reconstructed tracks from cosmic rays and from proton-proton collisions. The main track-based alignment method uses the Millepede global approach. An iterative local method was developed and used as well. We present the results obtained for the ITS alignment using about 10^5 charged tracks from cosmic rays that have been collected during summer 2008, with the ALICE solenoidal magnet switched off.Peer reviewe

Supermassive black hole winds in X-rays: SUBWAYS: II. HST UV spectroscopy of winds at intermediate redshifts

We present a UV spectroscopic study of ionized outflows in 21 active galactic nuclei (AGN), observed with the Hubble Space Telescope (HST). The targets of the Supermassive Black Hole Winds in X-rays (SUBWAYS) sample were selected with the aim to probe the parameter space of the underexplored AGN between the local Seyfert galaxies and the luminous quasars at high redshifts. Our targets, spanning redshifts of 0.1–0.4 and bolometric luminosities (Lbol) of 1045–1046 erg s-1, have been observed with a large multi-wavelength campaign using XMM-Newton, NuSTAR, and HST. Here, we model the UV spectra and look for different types of AGN outflows that may produce either narrow or broad UV absorption features. We examine the relations between the observed UV outflows and other properties of the AGN. We find that 60% of our targets show a presence of outflowing H¿I absorption, while 40% exhibit ionized outflows seen as absorption by either C¿IV, N¿V, or O¿VI. This is comparable to the occurrence of ionized outflows seen in the local Seyfert galaxies. All UV absorption lines in the sample are relatively narrow, with outflow velocities reaching up to -3300 km s-1. We did not detect any UV counterparts to the X-ray ultra-fast outflows (UFOs), most likely due to their being too highly ionized to produce significant UV absorption. However, all SUBWAYS targets with an X-ray UFO that have HST data demonstrate the presence of UV outflows at lower velocities. We find significant correlations between the column density (N) of the UV ions and Lbol of the AGN, with NH I decreasing with Lbol, while NO VI is increasing with Lbol. This is likely to be a photoionization effect, where toward higher AGN luminosities, the wind becomes more ionized, resulting in less absorption by neutral or low-ionization ions and more absorption by high-ionization ions. In addition, we find that N of the UV ions decreases as their outflow velocity increases. This may be explained by a mechanical power that is evacuating the UV-absorbing medium. Our observed relations are consistent with multiphase AGN feeding and feedback simulations indicating that a combination of both radiative and mechanical processes are in play.Peer ReviewedPostprint (published version