Cobertura da “Marcha da Família” (1964) 50 anos depois, uma análise do texto da folha de S. Paulo

Este trabalho faz uma análise textual da cobertura jornalística realizada pelo Jornal Folha de S.Paulo sobre a “Marcha da Família com Deus Pela Liberdade” manifestação popular ocorrida em março de 1964 contra as reformas de base assinadas pelo então presidente, João Goulart. A análise tem como objetivo detectar qual foi à posição do jornal (contra ou a favor) sobre a realização da marcha. Para isso foram analisadas as matérias e os editoriais do jornal entre os dias 16 de março de 1964 e 25 de março de 1964. A pesquisa consiste em verificar através das notícias veiculadas nesse jornal, a representação que ele construiu sobre esse período. Procuro mostrar como a Folha de S. Paulo retratou o contexto da “Marcha da Família” e qual a sua posição em relação a ela

Different Teaching Approaches and Use of Active Learning Strategies as Tools for Inter- and Transdisciplinary Education

This work reports a review on some of the ways in which education and research can be used to solve today’s complex problems by taking into account teaching and learning strategies that go beyond traditional teaching strategies. It revisits different teaching approaches and connects them directly to uni-, pluri-, multi-, inter-, and transdisciplinary attitudes, and how educational professionals think of the subject of teaching. This discussion reflects on how teachers tend to reproduce procedures they observed in their own graduation programs and why experienced teachers opt for inter- and transdisciplinary attitudes. Using a qualitative approach, this work illustrates the results from a Project-Based Learning strategy applied to a group of Major Engineering students to solve problems at the University’s Campus. Participants included 25 students engaged to Physics Engineering program supervised by different faculty members, experienced undergraduate and graduate students, and technicians, who had mentored the undergraduate students’ teams, all working in subjects closely related to the projects. The products developed by the teams show evidence that the students were motivated and engaged in the projects; this supports the premise that inter- and transdisciplinary approaches drive collaboration in the execution of projects, develop soft skills and permit knowledge development in an articulate and complex way thereby leading to a broader education of the students

A parallel approach to pca based malicious activitydetection in distributed honeypot data

Model order selection (MOS) schemes, which are frequently employed inseveral signal processing applications, are shown to be effective tools for the detectionof malicious activities in honeypot data. In this paper, we extend previous results byproposing an efficient and parallel MOS method for blind automatic malicious activitydetection in distributed honeypots. Our proposed scheme does not require any previousinformation on attacks or human intervention. We model network traffic data as signalsand noise and then apply modified signal processing methods. However, differently fromthe previous centralized solutions, we propose that the data colected by each honeypotnode be processed by nodes in a cluster (that may consist of the collection nodesthemselves) and then grouped to obtain the final results. This is achieved by having eachnode locally compute the Eigenvalue Decomposition (EVD) to its own sample correlationmatrix (obtained from the honeypot data) and transmit the resulting eigenvalues to acentral node, where the global eigenvalues and final model order are computed. Themodel order computed from the global eigenvalues through RADOI represents the numberof malicious activities detected in the analysed data. The feasibility of the proposedapproach is demonstrated through simulation experiments

Hormonal induction of ovulation and early weaning in postpartum fertility of homozigous and heterozigous beef cows for the microsatellite BMS3004

O objetivo deste experimento foi comparar a eficiência de um programa hormonal associado ao desmame temporário por 96 horas na indução do estro e ovulação com o desmame definitivo aos 60 dias em vacas de corte. Foram utilizadas 183 vacas de corte amamentando, das raças Charolês (C), Nelore (N) e suas cruzas recíprocas, as quais foram genotipadas como homozigotas (HOM) ou heterozigotas (HET) para o microssatélite (STR) BMS3004, que está localizado no mesmo cromossomo do gene da cadeia do LH. Entre 60 e 80 dias pós-parto (dia 0), as vacas foram distribuídas em dois grupos. No grupo indução hormonal (IH), as vacas (n=87) receberam (dia 0) 250 mg de acetato de medroxiprogesterona por 8 dias, 2,5 mg de benzoato de estradiol (dia 1) e 500 UI de gonadotrofina coriônica eqüina (dia 7). No dia 8, os bezerros foram desmamados por 96 horas. No mesmo dia (dia 8), as vacas (n=96) do outro grupo apenas foram submetidas ao desmame definitivo (grupo DP). Após, procedeu-se 4 dias de inseminação artificial (IA) e, passado esse período, foram entouradas. O primeiro diagnóstico de gestação (DG) foi realizado 60 dias após o período de IA e, o segundo, 60 dias após o final do entoure. As taxas de estro foram maiores nas vacas do grupo IH em relação as do grupo DP. As vacas com condição corporal 2,5 e 3,0 apresentaram menores percentuais de prenhez ao 1ºDG no grupo IH (29,6 e 46,4%) em relação ao grupo DP (56,0 e 72,2%). Os percentuais de prenhez das vacas com índice corporal 65-73 não diferiram entre os grupos IH e DP. As vacas N do grupo IH, apresentaram menor percentual de prenhez ao 1ºDG que as F1 (27,7 vs. 64,2%), mas não diferiram em relação às C (40,0%). No grupo IH, o percentual de prenhez ao 2ºDG foi menor nas vacas HOM do que nas HET. O desmame definitivo precoce mostrou-se mais eficaz no incremento dos percentuais de prenhez em vacas de corte. ___________________________________________________________________________________ ABSTRACTThe aim of this experiment was to compare the efficiency of a hormonal protocol, associated to the temporary weaning for 96 hours, with the definitive weaning at 60 days in beef cows, for the induction of estrus and ovulation. One hundred and eightythree suckled beef cows were used. The breeds of the cows were Charolais (C) and Nellore (N) and their crosses. The animals were genotyped as homozygous (HOM) and heterozigous (HET) for the microsatellite BMS3004, that is localized in the same chromossome of the LH chain gene. The cows were distributed in two groups between 60 and 80 days postpartum (day 0). In the hormonal induction group (HI), the cows (n=87) received (day 0) 250 mg of medroxiprogesteron acetate for 8 days, 2.5 mg of estradiol benzoate (day 1) and 500 UI of eCG (day 7). On day 8, the calves were weaned for 96 hours. In the same day (day 8), the cows (n=96) of the other group were just submitted to early weaning (group EW). Twelve hours after weaning, artificial insemination (AI) was done during four days. After this period, they were mated. The first diagnosis of pregnancy (DP) was performed 60 days after the AI period and, the second, 60 days after the end of mating. The estrus rates were higher in cows from HI group than in those of EW group. In the HI group, the cows with body condition 2.5 and 3.0 presented lower pregnancy rates at the 1st DP (29.6 and 46.4%) than in the EW group (56.0 and 72.2%). The rates of pregnancy in cows with body index 65-73 did not differ between the HI and EW groups. The N cows of HI group presented lower pregnancy rate at 1st DP than the F1 (27.7 vs. 64.2%), but was not different than the C cows (40.0%). In the HI group, the pregnancy rate at the 2nd DP was lower in HOM cows than in the HET ones. The cows in the early definitive weaning group showed to be more efficient than in the hormonal induction group to improve the pregnancy rate

Diversidade e potencial biotecnológico da comunidade bacteriana endofítica de sementes de soja

The objectives of this work were to isolate, characterize and identify the endophytic bacterial community of soybean seeds, and to test the biotechnological potential of this community. Seeds from 12 soybean cultivars were used. The endophytic bacterial isolates were evaluated for in vitro antagonism against phytopathogenic fungi, synthesis of indoleacetic acid (IAA), and capacity to solubilize phosphate. Isolation techniques, (ARDRA) grouping, and identification by means of partially sequencing the 16S rDNA were used in community characterization. The isolates with best biotechnological potential were inoculated in seeds to evaluate their ability to promote plant growth. Twelve ribotypes were identify by means of ARDRA and classified as: Acinetobacter, Bacillus, Brevibacterium, Chryseobacterium, Citrobacter, Curtobacterium, Enterobacter, Methylobacterium, Microbacterium, Micromonospora, Pantoea, Paenibacillus, Pseudomonas, Ochrobactrum, Streptomyces and Tsukamurella. As for the biotechnological potential of the community, 18% of the isolates were able to antagonize phytopathogenic fungi, 100% to synthesize IAA, and 39% to solubilize phosphate. The strain 67A(57) of Enterobacter sp. increased significantly the dry root biomass. Inoculation of promising isolates did not promote growth in soybean plants in most of the cases.O objetivo deste trabalho foi isolar, caracterizar e identificar a comunidade bacteriana endofítica de sementes de soja e avaliar o seu potencial biotecnológico. Foram utilizadas sementes de 12 cultivares de soja. Os isolados bacterianos endofíticos obtidos foram avaliados in vitro quanto ao antagonismo a fungos fitopatogênicos, síntese de ácido indolacético (AIA) e solubilização de fosfato. A caracterização foi realizada com técnicas de isolamento, análise de restrição do DNA ribossomal amplificado (ARDRA) e sequenciamento parcial do gene 16S rDNA. Os isolados com maior potencial biotecnológico foram inoculados em sementes de soja, para se avaliar a capacidade de promoção de crescimento de plantas. Foi possível identificar 12 ribótipos por meio da ARDRA, que foram classificados como: Acinetobacter, Bacillus, Brevibacterium, Chryseobacterium, Citrobacter, Curtobacterium, Enterobacter, Methylobacterium, Microbacterium, Micromonospora, Pantoea, Paenibacillus, Pseudomonas, Ochrobactrum, Streptomyces e Tsukamurella. Quanto ao potencial biotecnológico da comunidade, 18% dos isolados controlaram o crescimento de fungos fitopatogênicos, 100% produziram AIA, e 39% solubilizaram fosfato. O isolado 67A(57) de Enterobacter sp. aumentou significativamente a massa de matéria seca da raiz. A inoculação de isolados com elevado potencial biotecnológico em avaliações in vitro não promoveu o crescimento de plantas de soja na maioria dos casos

Cross-cultural adaptation of the City Birth Trauma Scale for the Brazilian context

Background: Posttraumatic stress disorder consists of a set of symptoms that occurs in response to one or more traumatic events and can occur in postpartum, from traumatic situations related to the birth or to the baby’s health in the first days of life. It is important tracking the presence of birth trauma, but there is not available instruments in the Brazilian context for this purpose. Objectives: To present the cross-cultural adaptation of City Birth Trauma Scale (BiTS) into Brazilian portuguese. Methods: Cross-cultural adaptation involved independent translations, synthesis,back-translation, and submission to the original author’s appreciation. After the scale was subjected to face validity, followed by a pilot study with postpartum mothers. Results: All steps were performed for the cross-cultural adaptation. Regarding face validity, items evaluated concerning different types of equivalence, presented satisfactory agreement values (≥4.20). Most of the expert’s suggestions were followed, being the main ones related to adjustments in prepositions, pronouns and verbal subjects. Pilot study showed that the mothers had been able to understand and respond to the instrument without adjustments. Discussion: BiTS’s Brazilian version proved to be cross-culturally adapted, ensuring the possibility of intercultural data comparison from the semantic, idiomatic, cultural, and conceptual perspectives. New studies are being conducted to attest its psychometric adequac

Portuguese Football Federation consensus statement 2020: nutrition and performance in football

Nutrition is an undeniable part of promoting health and performance among football (soccer) players. Nevertheless, nutritional strategies adopted in elite football can vary significantly depending on culture, habit and practical constraints and might not always be supported by scientific evidence. Therefore, a group of 28 Portuguese experts on sports nutrition, sports science and sports medicine sought to discuss current practices in the elite football landscape and review the existing evidence on nutritional strategies to be applied when supporting football players. Starting from understanding football's physical and physiological demands, five different moments were identified: preparing to play, match-day, recovery after matches, between matches and during injury or rehabilitation periods. When applicable, specificities of nutritional support to young athletes and female players were also addressed. The result is a set of practical recommendations that gathered consensus among involved experts, highlighting carbohydrates periodisation, hydration and conscious use of dietary supplements.info:eu-repo/semantics/publishedVersio

Comparative Effectiveness of COVID-19 Vaccines in Preventing Infections and Disease Progression from SARS-CoV-2 Omicron BA.5 and BA.2, Portugal

We estimated comparative primary and booster vaccine effectiveness (VE) of SARS-CoV-2 Omicron BA.5 and BA.2 lineages against infection and disease progression. During April-June 2022, we implemented a case-case and cohort study and classified lineages using whole-genome sequencing or spike gene target failure. For the case-case study, we estimated the adjusted odds ratios (aORs) of vaccination using a logistic regression. For the cohort study, we estimated VE against disease progression using a penalized logistic regression. We observed no reduced VE for primary (aOR 1.07 [95% CI 0.93-1.23]) or booster (aOR 0.96 [95% CI 0.84-1.09]) vaccination against BA.5 infection. Among BA.5 case-patients, booster VE against progression to hospitalization was lower than that among BA.2 case-patients (VE 77% [95% CI 49%-90%] vs. VE 93% [95% CI 86%-97%]). Although booster vaccination is less effective against BA.5 than against BA.2, it offers substantial protection against progression from BA.5 infection to severe disease.The acquisition of sequencing equipment and reagents used in this study by the Instituto Nacional de Saúde Doutor Ricardo Jorge was partially funded by the HERA project (grant no. 2021/PHF/23776) supported by the European Commission through the European Centre for Disease Control, and also partially funded by the GenomePT project (grant no. POCI-01-0145-FEDER-022184), supported by COMPETE 2020–Operational Programme for Competitiveness and Internationalisation, Lisboa Portugal Regional Operational Programme, Algarve Portugal Regional Operational, under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund, and by the Portuguese Science and Technology Foundation. Algarve Biomedical Center Laboratory received public funding through the Project ALG-D2-2021-06 Variants Screen in Southern Portugal– Monitoring Variants of Concern in Southern Portugal and the Portuguese Science and Technology Foundation national support through the Comprehensive Health Research Center (grant no. UIDP/04923/2020)info:eu-repo/semantics/publishedVersio

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio