Fibre types in skeletal muscles of chronic obstructive pulmonary disease patients related to respiratory function and exercise tolerance.

Abstract This study aimed to investigate the relationship between skeletal muscle, fibre type composition, functional respiratory impairment and exercise tolerance in patients with moderate to severe chronic obstructive pulmonary disease (COPD). A group of 22 COPD patients and 10 healthy control subjects were studied. In COPD patients, vital capacity (VC) and forced expiratory volume in one second (FEV1) were reduced to 79% and 51%, respectively. Diffusion indices (transfer factor of the lung for carbon monoxide (TL,CO) and carbon monoxide transfer coefficient (KCO)) were also reduced. Arterial oxygen tension (Pa,O2) was normal or slightly altered. A maximal exercise test was performed and anaerobic threshold was calculated. Muscle samples from vastus lateralis were obtained by needle biopsy. Myosin heavy chain (MHC) and light chain (MLC) isoforms were separated by gel electrophoresis and quantified by densitometry. MHC isoforms were considered as molecular markers of fibre types. The proportion of the fast MHC-2B isoform was increased in COPD patients. TL,CO, KCO, VC and FEV1 were positively correlated with slow MHC isoform content. TL,CO and KCO were also negatively correlated with the content of the fast MHC-2B isoform. No correlation was found between exercise parameters and MHC isoform composition. The co-ordinated expression between MHC and MLC isoforms was altered in COPD patients. We conclude that reduced oxygen availability, probably in combination with muscle disuse, may determine muscle alterations in chronic obstructive pulmonary disease patients. The altered correlations between myosin heavy chain and light chain isoforms suggest that co-ordinated protein expression is lost in chronic obstructive pulmonary disease muscles

Effect of the temperature and relative humidity in stored sotol (Dasylirion cedrosanum Trel.) seeds on fungi biodiversity

The objective of the research was to identify the fungi in sotol seeds at different conditions of temperature and relative humidity. Seeds were collected at Buñuelos, municipality, and taken to the Laboratory of the Center for Training and Development in Seed Technology (CCDTS) at Universidad Autonoma Agraria Antonio Narro. The seed was stored for a period of 90 days, whit conditions of 60, 75, 80 and 85% of relative humidity kept at 5, 15 and 25 °C. Fungi identifying by morphological criteria. A completely randomized experiment using R software, with factorial arrangement whit two replications. Pathogens identified were: Aspergillus glaucus, Aspergillus niger, Fusarium sp., Penicillium sp., Aspergillus candidus, Cladosporiun sp., Alternaria sp. and Aspergillus chraceus, the results showed that the higher the humidity, temperature and storage time, the incidence of fungi tends to be higher. Fungi with a higher presence in sotol seeds were: Aspergillus glaucus and Penicillium sp. Safe storage environments for sotol seeds reported in this work are 5 °C and a relative humidity of 60-75%. Sotol seeds tolerates conditions of 15 °C and a relative humidity up to 75%.&nbsp

Gas turbulence modulation in a two-fluid model for gas-solid flows

Recent rapid progress in the theoretical and experimental study of turbulence modulation has led to greater understanding of the physics of particle-gas turbulence interactions. A new two-fluid model incorporating these advances for relatively dilute gas-solid flows containing high-inertia particles is established. The effect of aerodynamic forces upon the particulate stresses is considered in this kinetic theory-based model, and the influence of the particles on the turbulent gas is addressed: the work associated with drag forces contributes to the gas turbulent energy, and the space occupied by particles restricts the turbulent length scale. The interparticle length scale, which is usually ignored, has been incorporated into a new model for determining the turbulent length scale. This model also considers the transport effect on the turbulent length scale. Simulation results for fully developed steady flows in vertical pipes are compared with a wide range of published experimental data and, generally, good agreement is shown. This comprehensive and validated model accounts for many of the interphase interactions that have been shown to be important

Multi-organ point-of-care ultrasound for COVID-19 (PoCUS4COVID): international expert consensus.

COVID-19 has caused great devastation in the past year. Multi-organ point-of-care ultrasound (PoCUS) including lung ultrasound (LUS) and focused cardiac ultrasound (FoCUS) as a clinical adjunct has played a significant role in triaging, diagnosis and medical management of COVID-19 patients. The expert panel from 27 countries and 6 continents with considerable experience of direct application of PoCUS on COVID-19 patients presents evidence-based consensus using GRADE methodology for the quality of evidence and an expedited, modified-Delphi process for the strength of expert consensus. The use of ultrasound is suggested in many clinical situations related to respiratory, cardiovascular and thromboembolic aspects of COVID-19, comparing well with other imaging modalities. The limitations due to insufficient data are highlighted as opportunities for future research.post-print2.282 K

Early impact of aortic wrapping on patients undergoing aortic valve replacement with mild to moderate ascending aorta dilatation

<p>Abstract</p> <p>Background</p> <p>The management of mild to moderate dilatation of the ascending aorta of less than 5 cm is controversial, particularly when concomitant surgical correction of aortic valve is required. We investigate the impact of a simple method of aorta reduction using Dacron graft wrapping during aortic valve replacement on the rest of the aorta.</p> <p>Methods</p> <p>We studied 14 patients who had ascending aorta dilatation of 4-5 cm before undergoing aortic wrapping during their aortic valve replacement and compared with their post-operative imaging within a month.</p> <p>Results</p> <p>The diameters of the ascending aorta wrapped with the Dacron graft were significantly reduced within 4 weeks after surgery from 44.7 ± 2.6 to 33.6 ± 3.9 mm (p < 0.001). This was associated with significant reduction in the diameter of rest of ascending aorta: coronary sinuses (from 37.9 ± 4.9 mm to 33.3 ± 6.1 mm; p < 0.001), sinotubular junction (from 33.2 ± 4.7 mm to 30.6 ± 4.4 mm, p = 0.02), and aortic arch (from 34.7 ± 4.3 mm to 32.6 ± 4.1 mm, p = 0.03).</p> <p>Conclusions</p> <p>Reduction of ascending aortic dilatation by wrapping with a Dacron graft in this preliminary study is associated with favourable early reversed aortic remodelling. This supports the hypothesis that correction of mild-moderate dilatation of the ascending aorta with Dacron wrapping at the time of aortic valve surgery may prevent the progression of the dilatation, although the long-term study on a larger population is needed to confirm its benefits.</p

Percepção de idosos sobre o papel do psicólogo em instituições de longa permanência

Este trabalho se propôs a descrever a percepção de idosos institucionalizados sobre o papel do profissional psicólogo junto à população abrigada das Instituições de Longa Permanência para Idosos (ILPI) da cidade de Juiz de Fora-MG. O universo da pesquisa abrangeu as instituições cuidadoras e cadastradas junto ao Conselho Municipal do Idoso da referida localidade. Quanto à metodologia, procedeu-se a estudo qualitativo exploratório utilizando a Análise de Conteúdo na perspectiva de Bardin como ferramenta para o tratamento dos dados. Os critérios de inclusão utilizados foram: 1) ser residente em ILPI; 2) ter 60 ou mais anos de idade; 3) aceitar participar; e 4) ser capaz de responder às perguntas formuladas. Constitui-se amostra por conveniência de 34 idosos que apresentaram as seguintes representações acerca do papel do psicólogo: relevância e pertinência de seu trabalho, não percebendo a presença de pontos negativos no trabalho deste profissional nas ILPIs. Identificou-se ainda que as áreas de atuação psicológica mais apontadas foram as relacionadas à prevenção, diagnóstico e tratamento dos residentes naqueles locais

Progressive Preference Articulation for Decision Making in Multi-Objective Optimisation Problems

This paper proposes a novel algorithm for addressing multi-objective optimisation problems, by employing a progressive preference articu- lation approach to decision making. This enables the interactive incorporation of problem knowledge and decision maker preferences during the optimisation process. A novel progressive preference articulation mechanism, derived from a statistical technique, is herein proposed and implemented within a multi-objective framework based on evolution strategy search and hypervolume indicator selection. The proposed algo- rithm is named the Weighted Z-score Covariance Matrix Adaptation Pareto Archived Evolution Strategy with Hypervolume-sorted Adaptive Grid Algorithm (WZ-HAGA). WZ-HAGA is based on a framework that makes use of evolution strategy logic with covariance matrix adaptation to perturb the solutions, and a hypervolume indicator driven algorithm to select successful solutions for the subsequent generation. In order to guide the search towards interesting regions, a preference articulation procedure composed of four phases and based on the weighted z-score approach is employed. The latter procedure cascades into the hypervolume driven algorithm to perform the selection of the solutions at each generation. Numerical results against five modern algorithms representing the state-of-the-art in multi-objective optimisation demonstrate that the pro- posed WZ-HAGA outperforms its competitors in terms of both the hypervolume indicator and pertinence to the regions of interest